{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T12:09:25Z","timestamp":1779451765436,"version":"3.53.1"},"reference-count":68,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"University Grants Commission","award":["F. No. 82-7\/2022(SA-III)."],"award-info":[{"award-number":["F. No. 82-7\/2022(SA-III)."]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Environ Monit Assess"],"DOI":"10.1007\/s10661-026-15375-1","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:58:03Z","timestamp":1777543083000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a modified protocol for extraction of environmental DNA from water samples to assess the presence of fish species in wetland ecosystems"],"prefix":"10.1007","volume":"198","author":[{"given":"Antareepa","family":"Saikia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Manu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grishma","family":"Tewari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuj","family":"Tyagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surjya Narayan","family":"Datta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarbjeet","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"15375_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rsma.2025.104557","volume":"91","author":"SI Ahmed","year":"2025","unstructured":"Ahmed, S. I., Yu, Z., Higuchi, T., Inoue, J., Wong, M.-S., Yoshizawa, S., et al. (2025). Fish species detection by environmental DNA metabarcoding by three surface sampling methods. Regional Studies in Marine Science, 91, Article 104557. https:\/\/doi.org\/10.1016\/j.rsma.2025.104557","journal-title":"Regional Studies in Marine Science"},{"issue":"2","key":"15375_CR2","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1111\/faf.12725","volume":"24","author":"RF Ainsworth","year":"2023","unstructured":"Ainsworth, R. F., Cowx, I. G., & Funge\u2010Smith, S. J. (2023). Putting the fish into inland fisheries \u2013 A global allocation of historic inland fish catch. Fish and Fisheries, 24(2), 263\u2013278. https:\/\/doi.org\/10.1111\/faf.12725","journal-title":"Fish and Fisheries"},{"issue":"3","key":"15375_CR3","doi-asserted-by":"publisher","first-page":"1819","DOI":"10.1021\/es404734p","volume":"48","author":"MA Barnes","year":"2014","unstructured":"Barnes, M. A., Turner, C. R., Jerde, C. L., Renshaw, M. A., Chadderton, W. L., & Lodge, D. M. (2014). Environmental conditions influence eDNA persistence in aquatic systems. Environmental Science & Technology, 48(3), 1819\u20131827. https:\/\/doi.org\/10.1021\/es404734p","journal-title":"Environmental Science & Technology"},{"key":"15375_CR4","doi-asserted-by":"publisher","unstructured":"Canene-Adams, K. (2013). General PCR. In Methods in Enzymology (Vol. 529, pp. 291\u2013298). Elsevier. https:\/\/doi.org\/10.1016\/B978-0-12-418687-3.00024-0","DOI":"10.1016\/B978-0-12-418687-3.00024-0"},{"issue":"Nov,11","key":"15375_CR5","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.23910\/1.2022.3238a","volume":"13","author":"A Chaudhari","year":"2022","unstructured":"Chaudhari, A., Felix, S., Swain, S. K., & Uma, A. (2022). Breeding of Indian spiny loach, Lepidocephalichthys thermalis (Valenciennes, 1846) under captive conditions. International Journal of Bio-Resource and Stress Management, 13(Nov,11), 1341\u20131347.","journal-title":"International Journal of Bio-Resource and Stress Management"},{"issue":"19","key":"15375_CR6","doi-asserted-by":"publisher","first-page":"4067","DOI":"10.1007\/s10750-023-05297-1","volume":"850","author":"R Cheng","year":"2023","unstructured":"Cheng, R., Luo, Y., Li, Q., Zhang, Y., Liu, Z., Chen, Q., et al. (2023). Application of eDNA metabarcoding for monitoring the fish diversity of the Jiangjin to Fuling section of the upper reaches of the Yangtze River. Hydrobiologia, 850(19), 4067\u20134088. https:\/\/doi.org\/10.1007\/s10750-023-05297-1","journal-title":"Hydrobiologia"},{"issue":"11","key":"15375_CR7","doi-asserted-by":"publisher","first-page":"1985","DOI":"10.1111\/2041-210X.13276","volume":"10","author":"RA Collins","year":"2019","unstructured":"Collins, R. A., Bakker, J., Wangensteen, O. S., Soto, A. Z., Corrigan, L., Sims, D. W., Genner, M. J., & Mariani, S. (2019). Non\u2010specific amplification compromises environmental DNA metabarcoding with COI. Methods in Ecology and Evolution, 10(11), 1985\u20132001. https:\/\/doi.org\/10.1111\/2041-210X.13276","journal-title":"Methods in Ecology and Evolution"},{"issue":"8","key":"15375_CR8","doi-asserted-by":"publisher","first-page":"2131","DOI":"10.1002\/aqc.3591","volume":"31","author":"MK Cooper","year":"2021","unstructured":"Cooper, M. K., Huerlimann, R., Edmunds, R. C., Budd, A. M., Le Port, A., Kyne, P. M., Jerry, D. R., & Simpfendorfer, C. A. (2021). Improved detection sensitivity using an optimal eDNA preservation and extraction workflow and its application to threatened sawfishes. Aquatic Conservation: Marine and Freshwater Ecosystems, 31(8), 2131\u20132148. https:\/\/doi.org\/10.1002\/aqc.3591","journal-title":"Aquatic Conservation: Marine and Freshwater Ecosystems"},{"key":"15375_CR9","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.biocon.2014.11.018","volume":"183","author":"K Deiner","year":"2015","unstructured":"Deiner, K., Walser, J.-C., M\u00e4chler, E., & Altermatt, F. (2015). Choice of capture and extraction methods affect detection of freshwater biodiversity from environmental DNA. Biological Conservation, 183, 53\u201363. https:\/\/doi.org\/10.1016\/j.biocon.2014.11.018","journal-title":"Biological Conservation"},{"issue":"4","key":"15375_CR10","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1111\/j.1365-2664.2012.02171.x","volume":"49","author":"T Dejean","year":"2012","unstructured":"Dejean, T., Valentini, A., Miquel, C., Taberlet, P., Bellemain, E., & Miaud, C. (2012). Improved detection of an alien invasive species through environmental DNA barcoding: The example of the American bullfrog Lithobates catesbeianus. Journal of Applied Ecology, 49(4), 953\u2013959. https:\/\/doi.org\/10.1111\/j.1365-2664.2012.02171.x","journal-title":"Journal of Applied Ecology"},{"issue":"1","key":"15375_CR11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-80907-z","volume":"14","author":"J Deng","year":"2024","unstructured":"Deng, J., Zhang, X., Yao, X., Rao, J., Dai, F., Wang, H., et al. (2024). eDNA metabarcoding reveals differences in fish diversity and community structure in Danjiang River. Scientific Reports, 14(1), Article 29460. https:\/\/doi.org\/10.1038\/s41598-024-80907-z","journal-title":"Scientific Reports"},{"issue":"2","key":"15375_CR12","first-page":"247","volume":"30","author":"A Dua","year":"2009","unstructured":"Dua, A., & Parkash, C. (2009). Distribution and abundance of fish populations in Harike Wetland-a Ramsar Site in India. Journal of Environmental Biology, 30(2), 247\u2013251.","journal-title":"Journal of Environmental Biology"},{"issue":"4","key":"15375_CR13","doi-asserted-by":"publisher","first-page":"1859","DOI":"10.1021\/acs.est.5b05672","volume":"50","author":"JJ Eichmiller","year":"2016","unstructured":"Eichmiller, J. J., Best, S. E., & Sorensen, P. W. (2016). Effects of temperature and trophic state on degradation of environmental DNA in lake water. Environmental Science & Technology, 50(4), 1859\u20131867. https:\/\/doi.org\/10.1021\/acs.est.5b05672","journal-title":"Environmental Science & Technology"},{"issue":"1","key":"15375_CR14","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1111\/1755-0998.12433","volume":"16","author":"NT Evans","year":"2016","unstructured":"Evans, N. T., Olds, B. P., Renshaw, M. A., Turner, C. R., Li, Y., Jerde, C. L., et al. (2016). Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding. Molecular Ecology Resources, 16(1), 29\u201341. https:\/\/doi.org\/10.1111\/1755-0998.12433","journal-title":"Molecular Ecology Resources"},{"key":"15375_CR15","doi-asserted-by":"publisher","unstructured":"Evans, N. T., & Lamberti, G. A. (2018). Freshwater fisheries assessment using environmental DNA: A primer on the method, its potential, and shortcomings as a conservation tool. Fisheries Research, 197, 60\u201366. https:\/\/doi.org\/10.1016\/j.fishres.2017.09.013","DOI":"10.1016\/j.fishres.2017.09.013"},{"issue":"1","key":"15375_CR16","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1002\/(SICI)1520-6017(200001)89:1<76::AID-JPS8>3.0.CO;2-U","volume":"89","author":"RK Evans","year":"2000","unstructured":"Evans, R. K., Xu, Z., Bohannon, K. E., Wang, B., Bruner, M. W., & Volkin, D. B. (2000). Evaluation of degradation pathways for plasmid DNA in pharmaceutical formulations via accelerated stability studies. Journal of Pharmaceutical Sciences, 89(1), 76\u201387. https:\/\/doi.org\/10.1002\/(SICI)1520-6017(200001)89:1%3C76::AID-JPS8%3E3.0.CO;2-U","journal-title":"Journal of Pharmaceutical Sciences"},{"issue":"1","key":"15375_CR17","doi-asserted-by":"publisher","DOI":"10.1016\/j.watbs.2022.100089","volume":"2","author":"X Feng","year":"2023","unstructured":"Feng, X., Li, B., Chen, Y., Zhu, R., Jia, Y., & Sui, X. (2023). Species-level monitoring of rare and invasive fishes using eDNA metabarcoding in the middle and upper Yarlung Zangbo River, Tibet. Water Biology and Security, 2(1), Article 100089.","journal-title":"Water Biology and Security"},{"issue":"1","key":"15375_CR18","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-60917-7","volume":"14","author":"ME Green","year":"2024","unstructured":"Green, M. E., Hardesty, B. D., Deagle, B. E., & Wilcox, C. (2024). Environmental DNA as a tool to reconstruct catch composition for longline fisheries vessels. Scientific Reports, 14(1), Article 10188. https:\/\/doi.org\/10.1038\/s41598-024-60917-7","journal-title":"Scientific Reports"},{"key":"15375_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolind.2023.111222","volume":"157","author":"S Gu","year":"2023","unstructured":"Gu, S., Deng, Y., Wang, P., Li, C., Shi, D., & Wang, S. (2023). Assessing riverine fish community diversity and stability by eDNA metabarcoding. Ecological Indicators, 157, Article 111222.","journal-title":"Ecological Indicators"},{"key":"15375_CR20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolind.2024.112538","volume":"166","author":"L Hao","year":"2024","unstructured":"Hao, L., Gu, K., Zhou, Y., An, J., Hu, W., Wu, Z., Shao, J., Pan, J., He, G., Liu, Q., & Hu, Z. (2024). Comparing diversity and structure of freshwater fish assemblages using environmental DNA and gillnetting methods: A case study of a large deep reservoir in East China. Ecological Indicators, 166, Article 112538. https:\/\/doi.org\/10.1016\/j.ecolind.2024.112538","journal-title":"Ecological Indicators"},{"key":"15375_CR21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolind.2022.108968","volume":"139","author":"W He","year":"2022","unstructured":"He, W., Xu, D., Liang, Y., Ren, L., & Fang, D. (2022). Using eDNA to assess the fish diversity and spatial characteristics in the Changjiang River-Shijiu Lake connected system. Ecological Indicators, 139, Article 108968. https:\/\/doi.org\/10.1016\/j.ecolind.2022.108968","journal-title":"Ecological Indicators"},{"issue":"6","key":"15375_CR22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179251","volume":"12","author":"R Hinlo","year":"2017","unstructured":"Hinlo, R., Gleeson, D., Lintermans, M., & Furlan, E. (2017). Methods to maximise recovery of environmental DNA from water samples. PLoS ONE, 12(6), Article e0179251. https:\/\/doi.org\/10.1371\/journal.pone.0179251","journal-title":"PLoS ONE"},{"issue":"1","key":"15375_CR23","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40977-w","volume":"9","author":"ME Hunter","year":"2019","unstructured":"Hunter, M. E., Ferrante, J. A., Meigs-Friend, G., & Ulmer, A. (2019). Improving eDNA yield and inhibitor reduction through increased water volumes and multi-filter isolation techniques. Scientific Reports, 9(1), Article 5259. https:\/\/doi.org\/10.1038\/s41598-019-40977-w","journal-title":"Scientific Reports"},{"key":"15375_CR24","doi-asserted-by":"publisher","unstructured":"Jayasankar, P., Pradeep, M. A., Mini, K. G., & Arunkumar, T. V. (2017). Environmental DNA (eDNA) metabarcoding approach in fisheries research in India. Marine Fisheries Information Service, 29\u201331. https:\/\/doi.org\/10.13140\/RG.2.2.16740.83845","DOI":"10.13140\/RG.2.2.16740.83845"},{"issue":"1","key":"15375_CR25","first-page":"232","volume":"3","author":"R Jindal","year":"2014","unstructured":"Jindal, R., Singh, H., & Sharma, C. (2014). Fish diversity of Pong Dam Reservoir and Harike Wetland. International Journal of Applied Science and Engineering Research, 3(1), 232\u2013240.","journal-title":"International Journal of Applied Science and Engineering Research"},{"issue":"1","key":"15375_CR26","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1007\/s42974-021-00073-4","volume":"23","author":"KD Joshi","year":"2022","unstructured":"Joshi, K. D., Pathak, A. K., Kumar, S., Dayal, R., Singh, A. K., Kumar, R., et al. (2022). Fish community diversity assessment of protected Saraiyaman Wetland in the Ganga River basin, India. Community Ecology, 23(1), 63\u201373. https:\/\/doi.org\/10.1007\/s42974-021-00073-4","journal-title":"Community Ecology"},{"key":"15375_CR27","doi-asserted-by":"publisher","unstructured":"Kaller, M. D., Kelso, W. E., & Trexler, J. C. (2013). Wetland fish monitoring and assessment. In J. T. Anderson & C. A. Davis (Eds.), Wetland Techniques: Volume 2: Organisms (pp. 197\u2013263). Springer Netherlands. https:\/\/doi.org\/10.1007\/978-94-007-6931-1_6","DOI":"10.1007\/978-94-007-6931-1_6"},{"issue":"5","key":"15375_CR28","doi-asserted-by":"publisher","first-page":"935","DOI":"10.5958\/2277-940x.2017.00142.5","volume":"7","author":"H Kaur","year":"2017","unstructured":"Kaur, H., Datta, S. N., & Singh, A. (2017). Fish catch composition and biodiversity indices at Harike Wetland-a Ramsar site in India. Journal of Animal Research, 7(5), 935\u2013941. https:\/\/doi.org\/10.5958\/2277-940x.2017.00142.5","journal-title":"Journal of Animal Research"},{"key":"15375_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2021.101238","volume":"8","author":"M Kawato","year":"2021","unstructured":"Kawato, M., Yoshida, T., Miya, M., Tsuchida, S., Nagano, Y., Nomura, M., Yabuki, A., Fujiwara, Y., & Fujikura, K. (2021). Optimization of environmental DNA extraction and amplification methods for metabarcoding of deep-sea fish. MethodsX, 8, Article 101238. https:\/\/doi.org\/10.1016\/j.mex.2021.101238","journal-title":"MethodsX"},{"issue":"6","key":"15375_CR30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0266720","volume":"17","author":"G Kumar","year":"2022","unstructured":"Kumar, G., Reaume, A. M., Farrell, E., & Gaither, M. R. (2022). Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary. PLoS One, 17(6), Article e0266720. https:\/\/doi.org\/10.1371\/journal.pone.0266720","journal-title":"PLoS One"},{"issue":"4","key":"15375_CR31","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1111\/1365-2664.12598","volume":"53","author":"A Lacoursi\u00e8re-Roussel","year":"2016","unstructured":"Lacoursi\u00e8re-Roussel, A., C\u00f4t\u00e9, G., Leclerc, V., & Bernatchez, L. (2016). Quantifying relative fish abundance with eDNA: A promising tool for fisheries management. Journal of Applied Ecology, 53(4), 1148\u20131157. https:\/\/doi.org\/10.1111\/1365-2664.12598","journal-title":"Journal of Applied Ecology"},{"issue":"1","key":"15375_CR32","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1139\/cjfas-2018-0263","volume":"77","author":"RF Lance","year":"2020","unstructured":"Lance, R. F., & Guan, X. (2020). Variation in inhibitor effects on qPCR assays and implications for eDNA surveys. Canadian Journal of Fisheries and Aquatic Sciences, 77(1), 23\u201333. https:\/\/doi.org\/10.1139\/cjfas-2018-0263","journal-title":"Canadian Journal of Fisheries and Aquatic Sciences"},{"issue":"3","key":"15375_CR33","doi-asserted-by":"publisher","first-page":"343","DOI":"10.3391\/mbi.2017.8.3.08","volume":"8","author":"R Lance","year":"2017","unstructured":"Lance, R., Klymus, K., Richter, C., Guan, X., Farrington, H., Carr, M., Thompson, N., Chapman, D., & Baerwaldt, K. (2017). Experimental observations on the decay of environmental DNA from bighead and silver carps. Management of Biological Invasions, 8(3), 343\u2013359. https:\/\/doi.org\/10.3391\/mbi.2017.8.3.08","journal-title":"Management of Biological Invasions"},{"issue":"5","key":"15375_CR34","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1111\/1755-0998.12899","volume":"18","author":"J Li","year":"2018","unstructured":"Li, J., Lawson Handley, L., Read, D. S., & H\u00e4nfling, B. (2018). The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding. Molecular Ecology Resources, 18(5), 1102\u20131114. https:\/\/doi.org\/10.1111\/1755-0998.12899","journal-title":"Molecular Ecology Resources"},{"issue":"1","key":"15375_CR35","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1046\/j.1472-765X.2003.01345.x","volume":"37","author":"C-H Liao","year":"2003","unstructured":"Liao, C.-H., & Shollenberger, L. M. (2003). Survivability and long-term preservation of bacteria in water and in phosphate-buffered saline*. Letters in Applied Microbiology, 37(1), 45\u201350. https:\/\/doi.org\/10.1046\/j.1472-765X.2003.01345.x","journal-title":"Letters in Applied Microbiology"},{"key":"15375_CR36","doi-asserted-by":"publisher","DOI":"10.3389\/fenvs.2024.1422269","volume":"12","author":"Q Liu","year":"2024","unstructured":"Liu, Q., Tan, J., Wang, M., Xin, N., Qi, R., & Wang, H. (2024). Optimization of pore size and filter material for better enrichment of environmental DNA. Frontiers in Environmental Science, 12, Article 1422269. https:\/\/doi.org\/10.3389\/fenvs.2024.1422269","journal-title":"Frontiers in Environmental Science"},{"issue":"3","key":"15375_CR37","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/S1355-0306(06)71591-X","volume":"46","author":"NC Martin","year":"2006","unstructured":"Martin, N. C., Pirie, A. A., Ford, L. V., Callaghan, C. L., McTurk, K., Lucy, D., & Scrimger, D. G. (2006). The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs. Science & Justice, 46(3), 179\u2013184. https:\/\/doi.org\/10.1016\/S1355-0306(06)71591-X","journal-title":"Science & Justice"},{"issue":"4","key":"15375_CR38","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1002\/edn3.188","volume":"3","author":"Q Mauvisseau","year":"2021","unstructured":"Mauvisseau, Q., Halfmaerten, D., Neyrinck, S., Burian, A., & Brys, R. (2021). Effects of preservation strategies on environmental DNA detection and quantification using ddPCR. Environmental DNA, 3(4), 815\u2013822. https:\/\/doi.org\/10.1002\/edn3.188","journal-title":"Environmental DNA"},{"issue":"2","key":"15375_CR39","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/s10201-011-0362-4","volume":"13","author":"T Minamoto","year":"2012","unstructured":"Minamoto, T., Yamanaka, H., Takahara, T., Honjo, M. N., & Kawabata, Z. (2012). Surveillance of fish species composition using environmental DNA. Limnology, 13(2), 193\u2013197. https:\/\/doi.org\/10.1007\/s10201-011-0362-4","journal-title":"Limnology"},{"issue":"7","key":"15375_CR40","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.150088","volume":"2","author":"M Miya","year":"2015","unstructured":"Miya, M., Sato, Y., Fukunaga, T., Sado, T., Poulsen, J. Y., Sato, K., et al. (2015). MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: Detection of more than 230 subtropical marine species. Royal Society Open Science, 2(7), Article 150088. https:\/\/doi.org\/10.1098\/rsos.150088","journal-title":"Royal Society Open Science"},{"key":"15375_CR41","unstructured":"Morgan, A. C., & Burgess, G. H. (2005). Fishery-development sampling: Total catch, effort and catch composition. In J. A. Musick & R. Bonfil (Eds.), Management techniques for elasmobranch fisheries (pp. 182\u2013200). Food and Agriculture Organization of the United Nations."},{"key":"15375_CR42","unstructured":"Moza, U., & Mishra, D. N. (2003). Harike wetland. Central Inland Fisheries Research Institute, Barrackpore.\u00a0Bulletin, (122)."},{"issue":"8","key":"15375_CR43","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.1139\/cjfas-2013-0047","volume":"70","author":"DS Pilliod","year":"2013","unstructured":"Pilliod, D. S., Goldberg, C. S., Arkle, R. S., & Waits, L. P. (2013). Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples. Canadian Journal of Fisheries and Aquatic Sciences, 70(8), 1123\u20131130. https:\/\/doi.org\/10.1139\/cjfas-2013-0047","journal-title":"Canadian Journal of Fisheries and Aquatic Sciences"},{"issue":"6","key":"15375_CR44","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1002\/edn3.232","volume":"3","author":"A Polanco F.","year":"2021","unstructured":"Polanco F., A., Richards, E., Fl\u00fcck, B., Valentini, A., Altermatt, F., Brosse, S., et al. (2021). Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems. Environmental DNA, 3(6), 1113\u20131127. https:\/\/doi.org\/10.1002\/edn3.232","journal-title":"Environmental DNA"},{"key":"15375_CR45","doi-asserted-by":"publisher","unstructured":"Qian, J., Lin, S., Wang, X., & Wang, H. (2023). First record of the non-native fish Rhinogobius cliffordpopei (Nichols, 1925) (Gobiiformes: Gobiidae) in Tibet, China. BioInvasions Records, 12(4), 1139\u20131146. https:\/\/doi.org\/10.3391\/bir.2023.12.4.25","DOI":"10.3391\/bir.2023.12.4.25"},{"issue":"5","key":"15375_CR46","doi-asserted-by":"publisher","first-page":"1450","DOI":"10.1111\/1365-2664.12306","volume":"51","author":"HC Rees","year":"2014","unstructured":"Rees, H. C., Maddison, B. C., Middleditch, D. J., Patmore, J. R. M., & Gough, K. C. (2014). REVIEW: The detection of aquatic animal species using environmental DNA \u2013 A review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51(5), 1450\u20131459. https:\/\/doi.org\/10.1111\/1365-2664.12306","journal-title":"Journal of Applied Ecology"},{"issue":"1","key":"15375_CR47","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1111\/1755-0998.12281","volume":"15","author":"MA Renshaw","year":"2015","unstructured":"Renshaw, M. A., Olds, B. P., Jerde, C. L., McVeigh, M. M., & Lodge, D. M. (2015). The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol\u2013chloroform\u2013isoamyl alcohol DNA extraction. Molecular Ecology Resources, 15(1), 168\u2013176. https:\/\/doi.org\/10.1111\/1755-0998.12281","journal-title":"Molecular Ecology Resources"},{"issue":"2","key":"15375_CR48","doi-asserted-by":"publisher","DOI":"10.1002\/eap.2273","volume":"31","author":"T Russo","year":"2021","unstructured":"Russo, T., Maiello, G., Talarico, L., Baillie, C., Colosimo, G., D\u2019Andrea, L., et al. (2021). All is fish that comes to the net: Metabarcoding for rapid fisheries catch assessment. Ecological Applications, 31(2), Article e02273. https:\/\/doi.org\/10.1002\/eap.2273","journal-title":"Ecological Applications"},{"key":"15375_CR49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnc.2022.126325","volume":"71","author":"A Sahu","year":"2023","unstructured":"Sahu, A., Kumar, N., Pal Singh, C., & Singh, M. (2023). Environmental DNA (eDNA): Powerful technique for biodiversity conservation. Journal for Nature Conservation, 71, Article 126325. https:\/\/doi.org\/10.1016\/j.jnc.2022.126325","journal-title":"Journal for Nature Conservation"},{"key":"15375_CR50","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2021.623831","volume":"9","author":"T Saito","year":"2021","unstructured":"Saito, T., & Doi, H. (2021). A model and simulation of the influence of temperature and amplicon length on environmental DNA degradation rates: A meta-analysis approach. Frontiers in Ecology and Evolution, 9, Article 623831. https:\/\/doi.org\/10.3389\/fevo.2021.623831","journal-title":"Frontiers in Ecology and Evolution"},{"issue":"5","key":"15375_CR51","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0233522","volume":"15","author":"TM Sanches","year":"2020","unstructured":"Sanches, T. M., & Schreier, A. D. (2020). Optimizing an eDNA protocol for estuarine environments: Balancing sensitivity, cost and time. PLoS ONE, 15(5), Article e0233522. https:\/\/doi.org\/10.1371\/journal.pone.0233522","journal-title":"PLoS ONE"},{"issue":"24","key":"15375_CR52","doi-asserted-by":"publisher","first-page":"14244","DOI":"10.1021\/acs.est.7b05199","volume":"51","author":"BJ Sansom","year":"2017","unstructured":"Sansom, B. J., & Sassoubre, L. M. (2017). Environmental DNA (eDNA) shedding and decay rates to model freshwater mussel eDNA transport in a river. Environmental Science & Technology, 51(24), 14244\u201314253. https:\/\/doi.org\/10.1021\/acs.est.7b05199","journal-title":"Environmental Science & Technology"},{"issue":"4","key":"15375_CR53","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.13942","volume":"24","author":"T Schenekar","year":"2024","unstructured":"Schenekar, T., Baxter, J., Phukuntsi, M. A., Sedlmayr, I., Weckworth, B., & Mwale, M. (2024). Optimizing waterborne eDNA capture from waterholes in savanna systems under remote field conditions. Molecular Ecology Resources, 24(4), Article e13942. https:\/\/doi.org\/10.1111\/1755-0998.13942","journal-title":"Molecular Ecology Resources"},{"issue":"5","key":"15375_CR54","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.1111\/j.1365-2672.2012.05384.x","volume":"113","author":"C Schrader","year":"2012","unstructured":"Schrader, C., Schielke, A., Ellerbroek, L., & Johne, R. (2012). PCR inhibitors\u2014Occurrence, properties and removal. Journal of Applied Microbiology, 113(5), 1014\u20131026. https:\/\/doi.org\/10.1111\/j.1365-2672.2012.05384.x","journal-title":"Journal of Applied Microbiology"},{"issue":"1","key":"15375_CR55","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.505","volume":"6","author":"SN Tabatabaei","year":"2024","unstructured":"Tabatabaei, S. N., Hashemzadeh Segherloo, I., Abdoli, A., Afzali, S. F., Normandeau, E., Laporte, M., et al. (2024). eDNA metabarcoding as a means to track distributions of different fish species in a protected area. Environmental DNA, 6(1), Article e505. https:\/\/doi.org\/10.1002\/edn3.505","journal-title":"Environmental DNA"},{"key":"15375_CR56","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780198767220.001.0001","volume-title":"Environmental DNA: For biodiversity research and monitoring","author":"P Taberlet","year":"2018","unstructured":"Taberlet, P., Bonin, A., Zinger, L., & Coissac, E. (2018). Environmental DNA: For biodiversity research and monitoring (1st ed.). Oxford University Press. https:\/\/doi.org\/10.1093\/oso\/9780198767220.001.0001","edition":"1st ed."},{"issue":"7","key":"15375_CR57","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1111\/2041-210X.12206","volume":"5","author":"CR Turner","year":"2014","unstructured":"Turner, C. R., Barnes, M. A., Xu, C. C., Jones, S. E., Jerde, C. L., & Lodge, D. M. (2014). Particle size distribution and optimal capture of aqueous macrobial eDNA. Methods in Ecology and Evolution, 5(7), 676\u2013684.","journal-title":"Methods in Ecology and Evolution"},{"issue":"4","key":"15375_CR58","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1111\/mec.13428","volume":"25","author":"A Valentini","year":"2016","unstructured":"Valentini, A., Taberlet, P., Miaud, C., Civade, R., Herder, J., Thomsen, P. F., et al. (2016). Next\u2010generation monitoring of aquatic biodiversity using environmental DNA metabarcoding. Molecular Ecology, 25(4), 929\u2013942. https:\/\/doi.org\/10.1111\/mec.13428","journal-title":"Molecular Ecology"},{"key":"15375_CR59","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2025.107859","volume":"278","author":"A Velmurugan","year":"2025","unstructured":"Velmurugan, A., Chidambaram, P., Velmurugan, P., Gokul, S., & Somu Sunder Lingam, R. (2025). Influence of water pH and temperature on breeding and embryo development of Indian spiny loach (Lepidocephalichthys thermalis). Animal Reproduction Science, 278, Article 107859. https:\/\/doi.org\/10.1016\/j.anireprosci.2025.107859","journal-title":"Animal Reproduction Science"},{"issue":"1462","key":"15375_CR60","doi-asserted-by":"publisher","first-page":"1847","DOI":"10.1098\/rstb.2005.1716","volume":"360","author":"RD Ward","year":"2005","unstructured":"Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R., & Hebert, P. D. N. (2005). DNA barcoding Australia\u2019s fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847\u20131857. https:\/\/doi.org\/10.1098\/rstb.2005.1716","journal-title":"Philosophical Transactions of the Royal Society B: Biological Sciences"},{"issue":"1","key":"15375_CR61","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-016-2104-5","volume":"9","author":"KE Williams","year":"2016","unstructured":"Williams, K. E., Huyvaert, K. P., & Piaggio, A. J. (2016). No filters, no fridges: A method for preservation of water samples for eDNA analysis. BMC Research Notes, 9(1), Article 298. https:\/\/doi.org\/10.1186\/s13104-016-2104-5","journal-title":"BMC Research Notes"},{"issue":"5","key":"15375_CR62","doi-asserted-by":"publisher","first-page":"1372","DOI":"10.1002\/nafm.10937","volume":"43","author":"D Williford","year":"2023","unstructured":"Williford, D., Hajovsky, P., & Anderson, J. (2023). Environmental DNA compliments traditional sampling for monitoring fish communities in a Texas estuary. North American Journal of Fisheries Management, 43(5), 1372\u20131394. https:\/\/doi.org\/10.1002\/nafm.10937","journal-title":"North American Journal of Fisheries Management"},{"key":"15375_CR63","doi-asserted-by":"publisher","unstructured":"Wong, M. K.-S., Nakao, M., & Hyodo, S. (2020). Field application of an improved protocol for environmental DNA extraction, purification, and measurement using Sterivex filter. Scientific Reports, 10(1), 21531. https:\/\/doi.org\/10.1038\/s41598-020-77304-7","DOI":"10.1038\/s41598-020-77304-7"},{"key":"15375_CR64","doi-asserted-by":"publisher","unstructured":"Wu, Q., & Minamoto, T. (2023). Improvement of recovery yield of macro-organismal environmental DNA from seawater samples. Analytical Sciences, 39(5), 713\u2013720. https:\/\/doi.org\/10.1007\/s44211-023-00280-1","DOI":"10.1007\/s44211-023-00280-1"},{"key":"15375_CR65","doi-asserted-by":"publisher","DOI":"10.3389\/fenvs.2024.1415338","volume":"12","author":"P Wu","year":"2024","unstructured":"Wu, P., Feng, J., Ju, M., Wu, S., Han, W., Wang, M., et al. (2024). Water filter: A rapid water environmental DNA collector in the field. Frontiers in Environmental Science, 12, Article 1415338. https:\/\/doi.org\/10.3389\/fenvs.2024.1415338","journal-title":"Frontiers in Environmental Science"},{"key":"15375_CR66","doi-asserted-by":"publisher","DOI":"10.3389\/fenvs.2022.725360","volume":"10","author":"Y Xing","year":"2022","unstructured":"Xing, Y., Gao, W., Shen, Z., Zhang, Y., Bai, J., Cai, X., et al. (2022). A review of environmental DNA field and laboratory protocols applied in fish ecology and environmental health. Frontiers in Environmental Science, 10, Article 725360. https:\/\/doi.org\/10.3389\/fenvs.2022.725360","journal-title":"Frontiers in Environmental Science"},{"issue":"1","key":"15375_CR67","doi-asserted-by":"publisher","DOI":"10.1016\/j.watbs.2022.100007","volume":"1","author":"F Xiong","year":"2022","unstructured":"Xiong, F., Shu, L., Zeng, H., Gan, X., He, S., & Peng, Z. (2022). Methodology for fish biodiversity monitoring with environmental DNA metabarcoding: The primers, databases and bioinformatic pipelines. Water Biology and Security, 1(1), Article 100007. https:\/\/doi.org\/10.1016\/j.watbs.2022.100007","journal-title":"Water Biology and Security"},{"issue":"12","key":"15375_CR68","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1111\/2041-210X.13485","volume":"11","author":"S Zhang","year":"2020","unstructured":"Zhang, S., Zhao, J., & Yao, M. (2020). A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish. Methods in Ecology and Evolution, 11(12), 1609\u20131625. https:\/\/doi.org\/10.1111\/2041-210X.13485","journal-title":"Methods in Ecology and Evolution"}],"container-title":["Environmental Monitoring and Assessment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10661-026-15375-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10661-026-15375-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10661-026-15375-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T11:37:07Z","timestamp":1779449827000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10661-026-15375-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":68,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["15375"],"URL":"https:\/\/doi.org\/10.1007\/s10661-026-15375-1","relation":{},"ISSN":["1573-2959"],"issn-type":[{"value":"1573-2959","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]},"assertion":[{"value":"8 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"533"}}