{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:06:59Z","timestamp":1789110419855,"version":"build-2803163510"},"reference-count":57,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100018802","name":"Universidade Federal Da Para\u00edba","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100018802","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Environ Monit Assess"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Carbapenemase-producing\n                    <jats:italic>Enterobacterales<\/jats:italic>\n                    (CPE) pose a significant public health concern due to the limited therapeutic options and increasing dissemination outside clinical settings. Wastewater treatment plants (WWTPs) have been proposed as relevant environmental reservoirs for antimicrobial-resistant bacteria and mobile genetic elements. In this study, we performed genomic characterization of CPE strains recovered from raw wastewater samples from the influents of different WWTPs. Antimicrobial susceptibility testing revealed multidrug resistance, including coresistance to carbapenems and polymyxins, among\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter asburiae<\/jats:italic>\n                    , and\n                    <jats:italic>Enterobacter kobei<\/jats:italic>\n                    strains. Whole-genome sequencing identified the convergence of the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>GES-16<\/jats:sub>\n                    genes, as well as the presence of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>GES-5<\/jats:sub>\n                    in\n                    <jats:italic>E. kobei<\/jats:italic>\n                    sequence type (ST) 540 strains. Moreover, the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    gene was detected in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strains belonging to high-risk clones ST11 (capsular types KL64 and KL15) and ST307 (capsular type KL102), and\n                    <jats:italic>E. asburiae<\/jats:italic>\n                    ST384. The\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>GES-5<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>GES-16<\/jats:sub>\n                    genes were associated with class 1 integrons, while the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    gene was embedded within transposons (Tn\n                    <jats:italic>4401a<\/jats:italic>\n                    , Tn\n                    <jats:italic>4401i<\/jats:italic>\n                    , and Tn\n                    <jats:italic>3<\/jats:italic>\n                    -like) and insertion sequences (IS\n                    <jats:italic>Kpn27<\/jats:italic>\n                    and IS\n                    <jats:italic>Kpn6<\/jats:italic>\n                    ). Notably, genomic analyses and literature review demonstrated that the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>GES-16<\/jats:sub>\n                    gene remains unique to Brazil. The putative pathogenic potential of carbapenem-resistant\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    ST11 was also assessed. These findings support the environmental circulation of clinically relevant\n                    <jats:italic>Enterobacterales<\/jats:italic>\n                    genotypes and emphasize the potential role of WWTPs as conduits for CPE dissemination, if not adequately operated. Therefore, genomic surveillance in extra-hospital settings may contribute to a better understanding of antimicrobial resistance ecology and inform One Health mitigation strategies.\n                  <\/jats:p>","DOI":"10.1007\/s10661-026-15867-0","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T16:17:18Z","timestamp":1788193038000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic characterization of carbapenemase-producing Enterobacterales from wastewater reveals the convergence of KPC-2 and GES-16 in Brazil"],"prefix":"10.1007","volume":"198","author":[{"given":"Jo\u00e3o Pedro Rueda","family":"Furlan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna Carrasco","family":"Bueno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubens Renato","family":"Sousa-Carmo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renan Louren\u00e7o Oliveira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikaela Renata Funada","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ines Zanoli","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Schenkman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"issue":"5","key":"15867_CR1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21051847","volume":"21","author":"A Amaretti","year":"2020","unstructured":"Amaretti, A., Righini, L., Candeliere, F., Musmeci, E., Bonvicini, F., Gentilomi, G. 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