{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T14:11:28Z","timestamp":1768399888060,"version":"3.49.0"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2012,10,6]],"date-time":"2012-10-06T00:00:00Z","timestamp":1349481600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glycoconj J"],"published-print":{"date-parts":[[2013,7]]},"DOI":"10.1007\/s10719-012-9451-9","type":"journal-article","created":{"date-parts":[[2012,10,5]],"date-time":"2012-10-05T16:36:10Z","timestamp":1349454970000},"page":"511-522","source":"Crossref","is-referenced-by-count":41,"title":["Engineering, conjugation, and immunogenicity assessment of Escherichia coli O121 O antigen for its potential use as a typhoid vaccine component"],"prefix":"10.1007","volume":"30","author":[{"given":"Michael","family":"Wetter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Kowarik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Steffen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Carranza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giampietro","family":"Corradin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Wacker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,10,6]]},"reference":[{"issue":"5","key":"9451_CR1","first-page":"346","volume":"82","author":"JA Crump","year":"2004","unstructured":"Crump, J.A., Luby, S.P., Mintz, E.D.: The global burden of typhoid fever. Bull. World Health Organ 82(5), 346\u2013353 (2004)","journal-title":"Bull. World Health Organ"},{"issue":"9487","key":"9451_CR2","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/S0140-6736(05)67181-4","volume":"366","author":"MK Bhan","year":"2005","unstructured":"Bhan, M.K., Bahl, R., Bhatnagar, S.: Typhoid and paratyphoid fever. Lancet 366(9487), 749\u2013762 (2005)","journal-title":"Lancet"},{"issue":"5","key":"9451_CR3","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1099\/00222615-44-5-317","volume":"44","author":"SH Mirza","year":"1996","unstructured":"Mirza, S.H., Beeching, N.J., Hart, C.A.: Multi-drug resistant typhoid: a global problem. J. Med. Microbiol. 44(5), 317\u2013319 (1996)","journal-title":"J. Med. Microbiol."},{"issue":"5","key":"9451_CR4","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1128\/IAI.56.5.1326-1333.1988","volume":"56","author":"DM Hone","year":"1988","unstructured":"Hone, D.M., Attridge, S.R., Forrest, B., Morona, R., Daniels, D., LaBrooy, J.T., Bartholomeusz, R.C., Shearman, D.J., Hackett, J.: A galE via (Vi antigen-negative) mutant of Salmonella typhi Ty2 retains virulence in humans. Infect. Immun. 56(5), 1326\u20131333 (1988)","journal-title":"Infect. Immun."},{"issue":"8541","key":"9451_CR5","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/S0140-6736(87)90480-6","volume":"1","author":"MM Levine","year":"1987","unstructured":"Levine, M.M., Ferreccio, C., Black, R.E., Germanier, R.: Large-scale field trial of Ty21a live oral typhoid vaccine in enteric-coated capsule formulation. Lancet 1(8541), 1049\u20131052 (1987)","journal-title":"Lancet"},{"issue":"1","key":"9451_CR6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0264-410X(90)90183-M","volume":"8","author":"RE Black","year":"1990","unstructured":"Black, R.E., Levine, M.M., Ferreccio, C., Clements, M.L., Lanata, C., Rooney, J., Germanier, R.: Efficacy of one or two doses of Ty21a Salmonella typhi vaccine in enteric-coated capsules in a controlled field trial. Chilean Typhoid Committee. Vaccine 8(1), 81\u201384 (1990)","journal-title":"Vaccine"},{"issue":"11","key":"9451_CR7","doi-asserted-by":"crossref","first-page":"4291","DOI":"10.1128\/IAI.59.11.4291-4293.1991","volume":"59","author":"JR Murphy","year":"1991","unstructured":"Murphy, J.R., Grez, L., Schlesinger, L., Ferreccio, C., Baqar, S., Munoz, C., Wasserman, S.S., Losonsky, G., Olson, J.G., Levine, M.M.: Immunogenicity of Salmonella typhi Ty21a vaccine for young children. Infect. Immun. 59(11), 4291\u20134293 (1991)","journal-title":"Infect. Immun."},{"issue":"8720","key":"9451_CR8","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/0140-6736(90)92266-K","volume":"336","author":"MM Levine","year":"1990","unstructured":"Levine, M.M., Ferreccio, C., Cryz, S., Ortiz, E.: Comparison of enteric-coated capsules and liquid formulation of Ty21a typhoid vaccine in randomised controlled field trial. Lancet 336(8720), 891\u2013894 (1990)","journal-title":"Lancet"},{"issue":"Suppl 2","key":"9451_CR9","doi-asserted-by":"crossref","first-page":"S22","DOI":"10.1016\/S0264-410X(99)00231-5","volume":"17","author":"MM Levine","year":"1999","unstructured":"Levine, M.M., Ferreccio, C., Abrego, P., Martin, O.S., Ortiz, E., Cryz, S.: Duration of efficacy of Ty21a, attenuated Salmonella typhi live oral vaccine. Vaccine 17(Suppl 2), S22\u2013S27 (1999)","journal-title":"Vaccine"},{"issue":"23","key":"9451_CR10","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1016\/j.carres.2003.07.008","volume":"338","author":"A Weintraub","year":"2003","unstructured":"Weintraub, A.: Immunology of bacterial polysaccharide antigens. Carbohydr. Res. 338(23), 2539\u20132547 (2003)","journal-title":"Carbohydr. Res."},{"issue":"1","key":"9451_CR11","first-page":"52","volume":"60","author":"M Landy","year":"1954","unstructured":"Landy, M.: Studies on Vi antigen. VI. Immunization of human beings with purified Vi antigen. Am. J. Hyg. 60(1), 52\u201362 (1954)","journal-title":"Am. J. Hyg."},{"issue":"10","key":"9451_CR12","doi-asserted-by":"crossref","first-page":"4440","DOI":"10.1128\/IAI.62.10.4440-4444.1994","volume":"62","author":"SC Szu","year":"1994","unstructured":"Szu, S.C., Taylor, D.N., Trofa, A.C., Clements, J.D., Shiloach, J., Sadoff, J.C., Bryla, D.A., Robbins, J.B.: Laboratory and preliminary clinical characterization of Vi capsular polysaccharide-protein conjugate vaccines. Infect. Immun. 62(10), 4440\u20134444 (1994)","journal-title":"Infect. Immun."},{"issue":"17","key":"9451_CR13","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1056\/NEJM200104263441701","volume":"344","author":"FY Lin","year":"2001","unstructured":"Lin, F.Y., Ho, V.A., Khiem, H.B., Trach, D.D., Bay, P.V., Thanh, T.C., Kossaczka, Z., Bryla, D.A., Shiloach, J., Robbins, J.B., Schneerson, R., Szu, S.C.: The efficacy of a Salmonella typhi Vi conjugate vaccine in two-to-five-year-old children. N. Engl. J. Med. 344(17), 1263\u20131269 (2001)","journal-title":"N. Engl. J. Med."},{"issue":"45","key":"9451_CR14","doi-asserted-by":"crossref","first-page":"42530","DOI":"10.1074\/jbc.M206114200","volume":"277","author":"NM Young","year":"2002","unstructured":"Young, N.M., Brisson, J.R., Kelly, J., Watson, D.C., Tessier, L., Lanthier, P.H., Jarrell, H.C., Cadotte, N., St Michael, F., Aberg, E., Szymanski, C.M.: Structure of the N-linked glycan present on multiple glycoproteins in the Gram-negative bacterium. Campylobacter jejuni. J. Biol. Chem. 277(45), 42530\u201342539 (2002)","journal-title":"J. Biol. Chem."},{"issue":"5599","key":"9451_CR15","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1126\/science.298.5599.1790","volume":"298","author":"M Wacker","year":"2002","unstructured":"Wacker, M., Linton, D., Hitchen, P.G., Nita-Lazar, M., Haslam, S.M., North, S.J., Panico, M., Morris, H.R., Dell, A., Wren, B.W., Aebi, M.: N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science (New York, N.Y) 298(5599), 1790\u20131793 (2002)","journal-title":"Science (New York, N.Y)"},{"issue":"8","key":"9451_CR16","doi-asserted-by":"crossref","first-page":"3016","DOI":"10.1073\/pnas.0500044102","volume":"102","author":"MF Feldman","year":"2005","unstructured":"Feldman, M.F., Wacker, M., Hernandez, M., Hitchen, P.G., Marolda, C.L., Kowarik, M., Morris, H.R., Dell, A., Valvano, M.A., Aebi, M.: Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 102(8), 3016\u20133021 (2005)","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"18","key":"9451_CR17","doi-asserted-by":"crossref","first-page":"7088","DOI":"10.1073\/pnas.0509207103","volume":"103","author":"M Wacker","year":"2006","unstructured":"Wacker, M., Feldman, M.F., Callewaert, N., Kowarik, M., Clarke, B.R., Pohl, N.L., Hernandez, M., Vines, E.D., Valvano, M.A., Whitfield, C., Aebi, M.: Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems. Proc. Natl. Acad. Sci. U. S. A. 103(18), 7088\u20137093 (2006)","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"9","key":"9451_CR18","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1038\/sj.emboj.7601087","volume":"25","author":"M Kowarik","year":"2006","unstructured":"Kowarik, M., Young, N.M., Numao, S., Schulz, B.L., Hug, I., Callewaert, N., Mills, D.C., Watson, D.C., Hernandez, M., Kelly, J.F., Wacker, M., Aebi, M.: Definition of the bacterial N-glycosylation site consensus sequence. EMBO J. 25(9), 1957\u20131966 (2006)","journal-title":"EMBO J."},{"issue":"12","key":"9451_CR19","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1128\/IAI.59.12.4555-4561.1991","volume":"59","author":"SC Szu","year":"1991","unstructured":"Szu, S.C., Li, X.R., Stone, A.L., Robbins, J.B.: Relation between structure and immunologic properties of the Vi capsular polysaccharide. Infect. Immun. 59(12), 4555\u20134561 (1991)","journal-title":"Infect. Immun."},{"key":"9451_CR20","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/S0076-6879(03)01079-6","volume":"363","author":"SC Szu","year":"2003","unstructured":"Szu, S.C., Bystricky, S.: Physical, chemical, antigenic, and immunologic characterization of polygalacturonan, its derivatives, and Vi antigen from Salmonella typhi. Methods Enzymol. 363, 552\u2013567 (2003)","journal-title":"Methods Enzymol."},{"key":"9451_CR21","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev.biochem.75.103004.142545","volume":"75","author":"C Whitfield","year":"2006","unstructured":"Whitfield, C.: Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. Annu. Rev. Biochem. 75, 39\u201368 (2006)","journal-title":"Annu. Rev. Biochem."},{"issue":"3","key":"9451_CR22","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0008-6215(97)00178-X","volume":"303","author":"H Parolis","year":"1997","unstructured":"Parolis, H., Parolis, L.A., Olivieri, G.: Structural studies on the Shigella-like Escherichia coli O121 O-specific polysaccharide. Carbohydr. Res. 303(3), 319\u2013325 (1997)","journal-title":"Carbohydr. Res."},{"issue":"6","key":"9451_CR23","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1111\/j.1462-2920.2010.02182.x","volume":"12","author":"JD King","year":"2010","unstructured":"King, J.D., Vinogradov, E., Tran, V., Lam, J.S.: Biosynthesis of uronamide sugars in Pseudomonas aeruginosa O6 and Escherichia coli O121 O antigens. Environ. Microbiol. 12(6), 1531\u20131544 (2010)","journal-title":"Environ. Microbiol."},{"issue":"Pt 1","key":"9451_CR24","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1099\/13500872-140-1-49","volume":"140","author":"D Liu","year":"1994","unstructured":"Liu, D., Reeves, P.R.: Escherichia coli K12 regains its O antigen. Microbiology 140(Pt 1), 49\u201357 (1994)","journal-title":"Microbiology"},{"issue":"49","key":"9451_CR25","doi-asserted-by":"crossref","first-page":"35129","DOI":"10.1074\/jbc.274.49.35129","volume":"274","author":"MF Feldman","year":"1999","unstructured":"Feldman, M.F., Marolda, C.L., Monteiro, M.A., Perry, M.B., Parodi, A.J., Valvano, M.A.: The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat. J. Biol. Chem. 274(49), 35129\u201335138 (1999)","journal-title":"J. Biol. Chem."},{"key":"9451_CR26","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1475-2859-9-61","volume":"9","author":"J Ihssen","year":"2010","unstructured":"Ihssen, J., Kowarik, M., Dilettoso, S., Tanner, C., Wacker, M., Thony-Meyer, L.: Production of glycoprotein vaccines in Escherichia coli. Microb. Cell. Fact. 9, 61 (2010)","journal-title":"Microb. Cell. Fact."},{"issue":"20","key":"9451_CR27","doi-asserted-by":"crossref","first-page":"6891","DOI":"10.1128\/JB.186.20.6891-6901.2004","volume":"186","author":"J Kubler-Kielb","year":"2004","unstructured":"Kubler-Kielb, J., Coxon, B., Schneerson, R.: Chemical structure, conjugation, and cross-reactivity of Bacillus pumilus Sh18 cell wall polysaccharide. J. Bacteriol. 186(20), 6891\u20136901 (2004)","journal-title":"J. Bacteriol."},{"issue":"25","key":"9451_CR28","doi-asserted-by":"crossref","first-page":"8709","DOI":"10.1073\/pnas.0803897105","volume":"105","author":"J Kubler-Kielb","year":"2008","unstructured":"Kubler-Kielb, J., Vinogradov, E., Hu, H., Leppla, S.H., Robbins, J.B., Schneerson, R.: Saccharides cross-reactive with Bacillus anthracis spore glycoprotein as an anthrax vaccine component. Proc. Natl. Acad. Sci. U. S. A. 105(25), 8709\u20138712 (2008)","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"9451_CR29","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0378-1119(82)90167-6","volume":"18","author":"AM Friedman","year":"1982","unstructured":"Friedman, A.M., Long, S.R., Brown, S.E., Buikema, W.J., Ausubel, F.M.: Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18(3), 289\u2013296 (1982)","journal-title":"Gene"},{"issue":"4","key":"9451_CR30","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/0022-2836(73)90198-8","volume":"80","author":"UK Laemmli","year":"1973","unstructured":"Laemmli, U.K., Favre, M.: Maturation of the head of bacteriophage T4. I. DNA packaging events. J. Mol. Biol. 80(4), 575\u2013599 (1973)","journal-title":"J. Mol. Biol."},{"issue":"1","key":"9451_CR31","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","volume":"119","author":"CM Tsai","year":"1982","unstructured":"Tsai, C.M., Frasch, C.E.: A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119(1), 115\u2013119 (1982)","journal-title":"Anal. Biochem."},{"issue":"40","key":"9451_CR32","doi-asserted-by":"crossref","first-page":"14255","DOI":"10.1073\/pnas.0507311102","volume":"102","author":"KJ Glover","year":"2005","unstructured":"Glover, K.J., Weerapana, E., Imperiali, B.: In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation. Proc. Natl. Acad. Sci. U. S. A. 102(40), 14255\u201314259 (2005)","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"2","key":"9451_CR33","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1006\/abio.1995.1468","volume":"230","author":"JC Bigge","year":"1995","unstructured":"Bigge, J.C., Patel, T.P., Bruce, J.A., Goulding, P.N., Charles, S.M., Parekh, R.B.: Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. Anal. Biochem. 230(2), 229\u2013238 (1995)","journal-title":"Anal. Biochem."},{"issue":"1","key":"9451_CR34","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1006\/abio.2002.5620","volume":"304","author":"AH Merry","year":"2002","unstructured":"Merry, A.H., Neville, D.C., Royle, L., Matthews, B., Harvey, D.J., Dwek, R.A., Rudd, P.M.: Recovery of intact 2-aminobenzamide-labeled O-glycans released from glycoproteins by hydrazinolysis. Anal. Biochem. 304(1), 91\u201399 (2002)","journal-title":"Anal. Biochem."},{"issue":"1","key":"9451_CR35","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.2002.5619","volume":"304","author":"L Royle","year":"2002","unstructured":"Royle, L., Mattu, T.S., Hart, E., Langridge, J.I., Merry, A.H., Murphy, N., Harvey, D.J., Dwek, R.A., Rudd, P.M.: An analytical and structural database provides a strategy for sequencing O-glycans from microgram quantities of glycoproteins. Anal. Biochem. 304(1), 70\u201390 (2002)","journal-title":"Anal. Biochem."},{"key":"9451_CR36","first-page":"3","volume":"431","author":"MA Apicella","year":"2008","unstructured":"Apicella, M.A.: Isolation and characterization of lipopolysaccharides. Methods Mol. Biol. 431, 3\u201313 (2008)","journal-title":"Methods Mol. Biol."},{"key":"9451_CR37","unstructured":"Demil, P., D\u2019Hondt, E., Hoecke, C.V.: Salmonella Typhi vaccine compositions. European Patent EP1107787 (2003)"},{"issue":"1","key":"9451_CR38","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0021-9258(18)56740-5","volume":"180","author":"S Hestrin","year":"1949","unstructured":"Hestrin, S.: The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application. J. Biol. Chem. 180(1), 249\u2013261 (1949)","journal-title":"J. Biol. Chem."},{"issue":"1\u20132","key":"9451_CR39","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0022-1759(98)00170-7","volume":"221","author":"A Frey","year":"1998","unstructured":"Frey, A., Di Canzio, J., Zurakowski, D.: A statistically defined endpoint titer determination method for immunoassays. J. Immunol. Methods 221(1\u20132), 35\u201341 (1998)","journal-title":"J. Immunol. Methods"},{"issue":"11","key":"9451_CR40","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/0264-410X(91)90218-U","volume":"9","author":"DM Hone","year":"1991","unstructured":"Hone, D.M., Harris, A.M., Chatfield, S., Dougan, G., Levine, M.M.: Construction of genetically defined double aro mutants of Salmonella typhi. Vaccine 9(11), 810\u2013816 (1991)","journal-title":"Vaccine"},{"issue":"1\u20132","key":"9451_CR41","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0378-1119(96)00289-2","volume":"177","author":"DM Dykxhoorn","year":"1996","unstructured":"Dykxhoorn, D.M., St Pierre, R., Linn, T.: A set of compatible tac promoter expression vectors. Gene 177(1\u20132), 133\u2013136 (1996)","journal-title":"Gene"}],"container-title":["Glycoconjugate Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10719-012-9451-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10719-012-9451-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10719-012-9451-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T01:36:26Z","timestamp":1714440986000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10719-012-9451-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,6]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["9451"],"URL":"https:\/\/doi.org\/10.1007\/s10719-012-9451-9","relation":{},"ISSN":["0282-0080","1573-4986"],"issn-type":[{"value":"0282-0080","type":"print"},{"value":"1573-4986","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,6]]}}}