{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:53:22Z","timestamp":1790628802219,"version":"4.1.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100008385","name":"Fondazione per la Ricerca sulla Fibrosi Cistica","doi-asserted-by":"crossref","award":["FFC#2\/2018, Delegazione FFC di Cuneo Alba"],"award-info":[{"award-number":["FFC#2\/2018, Delegazione FFC di Cuneo Alba"]}],"id":[{"id":"10.13039\/501100008385","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Glycoconj J"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human primary bronchial epithelial cells differentiated <jats:italic>in vitro<\/jats:italic> represent a valuable tool to study lung diseases such as cystic fibrosis (CF), an inherited disorder caused by mutations in the gene coding for the Cystic Fibrosis Transmembrane Conductance Regulator. In CF, sphingolipids, a ubiquitous class of bioactive lipids mainly associated with the outer layer of the plasma membrane, seem to play a crucial role in the establishment of the severe lung complications. Nevertheless, no information on the involvement of sphingolipids and their metabolism in the differentiation of primary bronchial epithelial cells are available so far. Here we show that ceramide and globotriaosylceramide increased during cell differentiation, whereas glucosylceramide and gangliosides content decreased. In addition, we found that apical plasma membrane of differentiated bronchial cells is characterized by a higher content of sphingolipids in comparison to the other cell membranes and that activity of sphingolipids catabolic enzymes associated with this membrane results altered with respect to the total cell activities. In particular, the apical membrane of CF cells was characterized by high levels of ceramide and glucosylceramide, known to have proinflammatory activity. On this basis, our data further support the role of sphingolipids in the onset of CF lung pathology.<\/jats:p>","DOI":"10.1007\/s10719-020-09935-x","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T08:02:42Z","timestamp":1594713762000},"page":"623-633","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Sphingolipids and plasma membrane hydrolases in human primary bronchial cells during differentiation and their altered patterns in cystic fibrosis"],"prefix":"10.1007","volume":"37","author":[{"given":"Nicoletta","family":"Loberto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Mancini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaria","family":"Bassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma Veronica","family":"Carsana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Tamanini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoletta","family":"Pedemonte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Dechecchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro","family":"Sonnino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Aureli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"key":"9935_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(85)90106-5","author":"K Uemura","year":"1985","unstructured":"Uemura, K., Macher, B.A., DeGregorio, M., Scudder, P., Buehler, J., Knapp, W., Feizi, T.: Glycosphingolipid carriers of carbohydrate antigens of human myeloid cells recognized by monoclonal antibodies. Biochim Biophys Acta (1985). https:\/\/doi.org\/10.1016\/0167-4889(85)90106-5","journal-title":"Biochim Biophys Acta ("},{"key":"9935_CR2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.09.022","author":"S Tivodar","year":"2006","unstructured":"Tivodar, S., Paladino, S., Pillich, R., Prinetti, A., Chigorno, V., van Meer, G., Sonnino, S., Zurzolo, C.: Analysis of detergent-resistant membranes associated with apical and basolateral GPI-anchored proteins in polarized epithelial cells. FEBS Lett. (2006). https:\/\/doi.org\/10.1016\/j.febslet.2006.09.022","journal-title":"FEBS Lett."},{"key":"9935_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2019.183145","author":"SCD van IJzendoorn","year":"2020","unstructured":"van IJzendoorn, S.C.D., Agnetti, J., Gassama-Diagne, A.: Mechanisms behind the polarized distribution of lipids in epithelial cells. Biochim Biophys Acta Biomembr (2020). https:\/\/doi.org\/10.1016\/j.bbamem.2019.183145","journal-title":"Biochim Biophys Acta Biomembr"},{"key":"9935_CR4","doi-asserted-by":"publisher","DOI":"10.1038\/35023524","author":"K R\u00f6per","year":"2000","unstructured":"R\u00f6per, K., Corbeil, D., Huttner, W.B.: Retention of prominin in microvilli reveals distinct cholesterol-based lipid micro-domains in the apical plasma membrane. Nat. Cell Biol. (2000). https:\/\/doi.org\/10.1038\/35023524","journal-title":"Nat. Cell Biol."},{"key":"9935_CR5","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20030235","author":"GH Hansen","year":"2003","unstructured":"Hansen, G.H., Pedersen, J., Niels-Christiansen, L.L., Immerdal, L., Danielsen, E.M.: Deep-apical tubules: dynamic lipid-raft microdomains in the brush-border region of enterocytes. Biochem. J. (2003). https:\/\/doi.org\/10.1042\/BJ20030235","journal-title":"Biochem. J."},{"key":"9935_CR6","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R800028-JLR200","author":"RK Yu","year":"2009","unstructured":"Yu, R.K., Nakatani, Y., Yanagisawa, M.: The role of glycosphingolipid metabolism in the developing brain. J Lipid Res (2009). https:\/\/doi.org\/10.1194\/jlr.R800028-JLR200","journal-title":"J Lipid Res"},{"key":"9935_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(86)90244-4","author":"M Hanqing","year":"1986","unstructured":"Hanqing, M., Avrova, N., M\u00e5nsson, J.E., Molin, K., Svennerholm, L.: Gangliosides and neutral glycosphingolipids of normal tissue and oat cell carcinoma of human lung. Biochim. Biophys. Acta. (1986). https:\/\/doi.org\/10.1016\/0005-2760(86)90244-4","journal-title":"Biochim. Biophys. Acta"},{"key":"9935_CR8","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29362","author":"JW Lee","year":"2015","unstructured":"Lee, J.W., Ryu, J.Y., Yoon, G., Jeon, H.K., Cho, Y.J., Choi, J.J., Song, S.Y., Do, I.G., Lee, Y.Y., Kim, T.J., Choi, C.H., Kim, B.G., Bae, D.S.: Sphingosine kinase 1 as a potential therapeutic target in epithelial ovarian cancer. Int J Cancer (2015). https:\/\/doi.org\/10.1002\/ijc.29362","journal-title":"Int J Cancer"},{"key":"9935_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/nm1238","author":"I Petrache","year":"2005","unstructured":"Petrache, I., Natarajan, V., Zhen, L., Medler, T.R., Richter, A.T., Cho, C., Hubbard, W.C., Berdyshev, E.V., Tuder, R.M.: Ceramide upregulation causes pulmonary cell apoptosis and emphysema-like disease in mice. Nat Med (2005). https:\/\/doi.org\/10.1038\/nm1238","journal-title":"Nat Med"},{"key":"9935_CR10","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0274OC","author":"TR Medler","year":"2008","unstructured":"Medler, T.R., Petrusca, D.N., Lee, P.J., Hubbard, W.C., Berdyshev, E.V., Skirball, J., Kamocki, K., Schuchman, E., Tuder, R.M.: Petrache, I. Apoptotic sphingolipid signaling by ceramides in lung endothelial cells. Am J Respir Cell Mol Biol (2008). https:\/\/doi.org\/10.1165\/rcmb.2007-0274OC","journal-title":"Am J Respir Cell Mol Biol"},{"key":"9935_CR11","doi-asserted-by":"crossref","unstructured":"Riordan, J.R., Rommens, J.M., Kerem, B., Alon, N., Rozmahel, R., Grzelczak, Z., Zielenski, J., Lok, S., Plavsic, N., Chou, J.L., et al.: Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245(4922), 1066\u201373 (1989)","DOI":"10.1126\/science.2475911"},{"key":"9935_CR12","doi-asserted-by":"publisher","DOI":"10.1002\/humu.10041","author":"JL Bobadilla","year":"2002","unstructured":"Bobadilla, J.L., Macek, M. Jr., Fine, J.P., Farrell, P.M.: Cystic fibrosis: a worldwide analysis of CFTR mutations\u2013correlation with incidence data and application to screening. Hum. Mutat. (2002) https:\/\/doi.org\/10.1002\/humu.10041","journal-title":"Hum. Mutat."},{"key":"9935_CR13","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2010-0457OC","author":"MC Dechecchi","year":"2011","unstructured":"Dechecchi, M.C., Nicolis, E., Mazzi, P., Cioffi, F., Bezzerri, V., Lampronti, I., Huang, S., Wiszniewski, L., Gambari, R., Scupoli, M.T., Berton, G., Cabrini, G.: Modulators of sphingolipid metabolism reduce lung inflammation. Am J Respir Cell Mol Biol (2011) https:\/\/doi.org\/10.1165\/rcmb.2010-0457OC","journal-title":"Am J Respir Cell Mol Biol"},{"key":"9935_CR14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104763","author":"N Loberto","year":"2014","unstructured":"Loberto, N., Tebon, M., Lampronti, I., Marchetti, N., Aureli, M., Bassi, R., Giri, M.G., Bezzerri, V., Lovato, V., Cant\u00f9, C., Munari, S., Cheng, S.H., Cavazzini, A., Gambari, R., Sonnino, S., Cabrini, G., Dechecchi, M.C.: GBA2-encoded \u03b2-glucosidase activity is involved in the inflammatory response to Pseudomonas aeruginosa. PLoS One (2014). https:\/\/doi.org\/10.1371\/journal.pone.0104763","journal-title":"PLoS One"},{"key":"9935_CR15","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1730245","author":"D Schiumarini","year":"2017","unstructured":"Schiumarini, D., Loberto, N., Mancini, G., Bassi, R., Giussani, P., Chiricozzi, E., Samarani, M., Munari, S., Tamanini, A., Cabrini, G., Lippi, G., Dechecchi, M.C., Sonnino, S.: Aureli, M. Evidence for the Involvement of Lipid Rafts and Plasma Membrane Sphingolipid Hydrolases in Pseudomonas aeruginosa Infection of Cystic Fibrosis Bronchial Epithelial Cells. Mediators Inflamm. (2017) https:\/\/doi.org\/10.1155\/2017\/1730245","journal-title":"Mediators Inflamm."},{"key":"9935_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.04.015","author":"KA Becker","year":"2018","unstructured":"Becker, K.A., Riethm\u00fcller, J., Seitz, A.P., Gardner, A., Boudreau, R., Kamler, M., Kleuser, B., Schuchman, E., Caldwell, C.C., Edwards, M.J., Grassm\u00e9, H., Brodlie, M., Gulbins, E.: Sphingolipids as targets for inhalation treatment of cystic fibrosis. Adv Drug Deliv Rev (2018) https:\/\/doi.org\/10.1016\/j.addr.2018.04.015","journal-title":"Adv Drug Deliv Rev"},{"key":"9935_CR17","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2016.08.002","author":"M Aureli","year":"2016","unstructured":"Aureli, M., Schiumarini, D., Loberto, N., Bassi, R., Tamanini, A., Mancini, G., Tironi, M., Munari, S., Cabrini, G., Dechecchi, M.C., Sonnino, S.: Unravelling the role of sphingolipids in cystic fibrosis lung disease. Chem Phys Lipids (2016). https:\/\/doi.org\/10.1016\/j.chemphyslip.2016.08.002","journal-title":"Chem Phys Lipids"},{"key":"9935_CR18","doi-asserted-by":"publisher","DOI":"10.33594\/000000208","author":"A Mingione","year":"2020","unstructured":"Mingione, A., Dei Cas, M., Bonezzi, F., Caretti, A., Piccoli, M., Anastasia, L., Ghidoni, R., Paroni, R., Signorelli, P.: Inhibition of Sphingolipid Synthesis as a Phenotype-Modifying Therapy in Cystic Fibrosis. Cell Physiol Biochem (2020) https:\/\/doi.org\/10.33594\/000000208","journal-title":"Cell Physiol Biochem"},{"key":"9935_CR19","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.240838","author":"P Scudieri","year":"2012","unstructured":"Scudieri, P., Caci, E., Bruno, S., Ferrera, L., Schiavon, M., Sondo, E., Tomati, V., Gianotti, A., Zegarra-Moran, O., Pedemonte, N., Rea, F., Ravazzolo, R., Galietta, L.J.: Association of TMEM16A chloride channel overexpression with airway goblet cell metaplasia. J. Physiol. (2012). https:\/\/doi.org\/10.1113\/jphysiol.2012.240838","journal-title":"J. Physiol."},{"key":"9935_CR20","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a027912","author":"MD Garcia-Castillo","year":"2017","unstructured":"Garcia-Castillo, M.D., Chinnapen, D.J., Lencer, W.I.: Membrane Transport across Polarized Epithelia. Cold Spring Harb. Perspect. Biol. (2017). https:\/\/doi.org\/10.1101\/cshperspect.a027912","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"9935_CR21","doi-asserted-by":"publisher","DOI":"10.1159\/000362983","author":"H Grassm\u00e9","year":"2014","unstructured":"Grassm\u00e9, H., Carpinteiro, A., Edwards, M.J., Gulbins, E., Becker, K.A.: Regulation of the inflammasome by ceramide in cystic fibrosis lungs. Cell Physiol Biochem (2014) https:\/\/doi.org\/10.1159\/000362983","journal-title":"Cell Physiol Biochem"},{"key":"9935_CR22","doi-asserted-by":"publisher","DOI":"10.1159\/000464382","author":"B Kovacic","year":"2017","unstructured":"Kovacic, B., Sehl, C., Wilker, B., Kamler, M., Gulbins, E., Becker, K.A.: Glucosylceramide Critically Contributes to the Host Defense of Cystic Fibrosis Lungs. Cell Physiol Biochem (2017) https:\/\/doi.org\/10.1159\/000464382","journal-title":"Cell Physiol Biochem"},{"key":"9935_CR23","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0288","author":"R Hajj","year":"2007","unstructured":"Hajj, R., Baranek, T., Le Naour, R., Lesimple, P., Puchelle, E., Coraux, C.: Basal cells of the human adult airway surface epithelium retain transit-amplifying cell properties. Stem Cells (2007) https:\/\/doi.org\/10.1634\/stemcells.2006-0288","journal-title":"Stem Cells"},{"key":"9935_CR24","doi-asserted-by":"publisher","unstructured":"Nitta, T., Kanoh, H., Inamori, K.I., Suzuki, A., Takahashi, T., Inokuchi, J.I. Globo-series glycosphingolipids enhance Toll-like receptor 4-mediated inflammation and play a pathophysiological role in diabetic nephropathy. Glycobiology: (2019) https:\/\/doi.org\/10.1093\/glycob\/cwy105","DOI":"10.1093\/glycob\/cwy105"},{"key":"9935_CR25","doi-asserted-by":"publisher","DOI":"10.1021\/cr0100446","author":"S Sonnino","year":"2006","unstructured":"Sonnino, S., Prinetti, A., Mauri, L., Chigorno, V., Tettamanti, G.: Dynamic and structural properties of sphingolipids as driving forces for the formation of membrane domains. Chem. Rev. (2006). https:\/\/doi.org\/10.1021\/cr0100446","journal-title":"Chem. Rev."},{"key":"9935_CR26","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3170-5_10","author":"M Aureli","year":"2016","unstructured":"Aureli, M., Grassi, S., Sonnino, S., Prinetti, A.: Isolation and Analysis of Detergent-Resistant Membrane Fractions. Methods Mol. Biol. (2016). https:\/\/doi.org\/10.1007\/978-1-4939-3170-5_10","journal-title":"Methods Mol. Biol."},{"key":"9935_CR27","doi-asserted-by":"publisher","DOI":"10.3390\/v3122462","author":"E Delorme-Axford","year":"2011","unstructured":"Delorme-Axford, E., Coyne, C.B.: The actin cytoskeleton as a barrier to virus infection of polarized epithelial cells. Viruses (2011). https:\/\/doi.org\/10.3390\/v3122462","journal-title":"Viruses"},{"key":"9935_CR28","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-21162-2_6","author":"AE Ventura","year":"2019","unstructured":"Ventura, A.E., Mestre, B., Silva, L.C.: Ceramide Domains in Health and Disease: A Biophysical Perspective. Adv. Exp. Med. Biol. (2019). https:\/\/doi.org\/10.1007\/978-3-030-21162-2_6","journal-title":"Adv. Exp. Med. Biol."},{"key":"9935_CR29","doi-asserted-by":"publisher","DOI":"10.1039\/c6cp07227e","author":"AR Varela","year":"2016","unstructured":"Varela, A.R., Ventura, A.E., Carreira, A.C., Fedorov, A., Futerman, A.H., Prieto, M., Silva, L.C.: Pathological levels of glucosylceramide change the biophysical properties of artificial and cell membranes. Phys. Chem. Chem. Phys. (2016). https:\/\/doi.org\/10.1039\/c6cp07227e","journal-title":"Phys. Chem. Chem. Phys."},{"key":"9935_CR30","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0725-1","author":"M Aureli","year":"2012","unstructured":"Aureli, M., Loberto, N., Bassi, R., Ferraretto, A., Perego, S., Lanteri, P., Chigorno, V., Sonnino, S., Prinetti, A.: Plasma membrane-associated glycohydrolases activation by extracellular acidification due to proton exchangers. Neurochem Res (2012). https:\/\/doi.org\/10.1007\/s11064-012-0725-1","journal-title":"Neurochem Res"},{"key":"9935_CR31","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0719-z","author":"M Aureli","year":"2012","unstructured":"Aureli, M., Gritti, A., Bassi, R., Loberto, N., Ricca, A., Chigorno, V., Prinetti, A., Sonnino, S.: Plasma membrane-associated glycohydrolases along differentiation of murine neural stem cells. Neurochem Res (2012). https:\/\/doi.org\/10.1007\/s11064-012-0719-z","journal-title":"Neurochem Res"},{"key":"9935_CR32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03397.x","author":"N Loberto","year":"2005","unstructured":"Loberto, N., Prioni, S., Bettiga, A., Chigorno, V., Prinetti, A., Sonnino, S.: The membrane environment of endogenous cellular prion protein in primary rat cerebellar neurons. J. Neurochem. (2005). https:\/\/doi.org\/10.1111\/j.1471-4159.2005.03397.x","journal-title":"J. Neurochem."},{"key":"9935_CR33","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.06.048","author":"M Aureli","year":"2009","unstructured":"Aureli, M., Masilamani, A.P., Illuzzi, G., Loberto, N., Scandroglio, F., Prinetti, A., Chigorno, V., Sonnino, S.: Activity of plasma membrane beta-galactosidase and beta-glucosidase. FEBS Lett. (2009) https:\/\/doi.org\/10.1016\/j.febslet.2009.06.048","journal-title":"FEBS Lett."},{"key":"9935_CR34","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.41.26522","author":"HS Overkleeft","year":"1998","unstructured":"Overkleeft, H.S., Renkema, G.H., Neele, J., Vianello, P., Hung, I.O., Strijland, A., van der Burg, A.M., Koomen, G.J., Pandit, U.K., Aerts, J.M.: Generation of specific deoxynojirimycin-type inhibitors of the non-lysosomal glucosylceramidase. J. Biol. Chem. (1998). https:\/\/doi.org\/10.1074\/jbc.273.41.26522","journal-title":"J. Biol. Chem."},{"key":"9935_CR35","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.463562","author":"CM Ridley","year":"2013","unstructured":"Ridley, C.M., Thur, K.E., Shanahan, J., Thillaiappan, N.B., Shen, A., Uhl, K., Walden, C.M., Rahim, A.A., Waddington, S.N., Platt, F.M., van der Spoel, A.C.: \u03b2-Glucosidase 2 (GBA2) activity and imino sugar pharmacology. J. Biol. Chem. (2013) https:\/\/doi.org\/10.1074\/jbc.M113.463562","journal-title":"J. Biol. Chem."},{"key":"9935_CR36","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M001974","author":"M Aureli","year":"2010","unstructured":"Aureli, M., Prioni, S., Mauri, L., Loberto, N., Casellato, R., Ciampa, M.G., Chigorno, V., Prinetti, A., Sonnino, S.: Photoactivable sphingosine as a tool to study membrane microenvironments in cultured cells. J Lipid Res (2010). https:\/\/doi.org\/10.1194\/jlr.M001974","journal-title":". J Lipid Res"},{"key":"9935_CR37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05591.x","author":"F Scandroglio","year":"2008","unstructured":"Scandroglio, F., Venkata, J.K., Loberto, N., Prioni, S., Schuchman, E.H., Chigorno, V., Prinetti, A., Sonnino, S.: Lipid content of brain, brain membrane lipid domains, and neurons from acid sphingomyelinase deficient mice. J. Neurochem. (2008) https:\/\/doi.org\/10.1111\/j.1471-4159.2008.05591.x","journal-title":"J. Neurochem."},{"key":"9935_CR38","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1002\/jlcr.2580290508","volume":"29","author":"T Toyokuni","year":"1991","unstructured":"Toyokuni, T., Nisar, M., Dean, B., Hakomori, S.-I.: A facile and regiospecific tritiation of sphingosine: synthesis of (2S,3R,4E)-2-amino-4-octadecene-1,3-diol-1-3H. J. Labelled Comp. Radiopharm 29, 567\u2013574 (1991)","journal-title":"J. Labelled Comp. Radiopharm"},{"key":"9935_CR39","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1054","author":"JG Altin","year":"1995","unstructured":"Altin, J.G., Pagler, E.B.: A one-step procedure for biotinylation and chemical cross-linking of lymphocyte surface and intracellular membrane-associated molecules. Anal. Biochem. (1995) https:\/\/doi.org\/10.1006\/abio.1995.1054","journal-title":"Anal. Biochem."},{"key":"9935_CR40","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(87)90051-4","author":"SR Cole","year":"1987","unstructured":"Cole, S.R., Ashman, L.K., Ey, P.L.: Biotinylation: an alternative to radioiodination for the identification of cell surface antigens in immunoprecipitates. Mol Immunol (1987), https:\/\/doi.org\/10.1016\/0161-5890(87)90051-4","journal-title":"Mol Immunol"},{"key":"9935_CR41","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00515.x","author":"A Prinetti","year":"2001","unstructured":"Prinetti, A., Prioni, S., Chigorno, V., Karagogeos, D., Tettamanti, G., Sonnino, S.: Immunoseparation of sphingolipid-enriched membrane domains enriched in Src family protein tyrosine kinases and in the neuronal adhesion molecule TAG-1 by anti-GD3 ganglioside monoclonal antibody. J. Neurochem. (2001). https:\/\/doi.org\/10.1046\/j.1471-4159.2001.00515.x","journal-title":"J. Neurochem."},{"key":"9935_CR42","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90189-j","author":"DA Brown","year":"1992","unstructured":"Brown, D.A., Rose, J.K.: Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell (1992). https:\/\/doi.org\/10.1016\/0092-8674(92)90189-j","journal-title":"Cell"},{"key":"9935_CR43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.07019.x","author":"M Aureli","year":"2011","unstructured":"Aureli, M., Loberto, N., Lanteri, P., Chigorno, V., Prinetti, A., Sonnino, S.: Cell surface sphingolipid glycohydrolases in neuronal differentiation and aging in culture. J Neurochem (2011). https:\/\/doi.org\/10.1111\/j.1471-4159.2010.07019.x","journal-title":". J Neurochem"}],"container-title":["Glycoconjugate Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10719-020-09935-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10719-020-09935-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10719-020-09935-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T23:54:41Z","timestamp":1626220481000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10719-020-09935-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,14]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["9935"],"URL":"https:\/\/doi.org\/10.1007\/s10719-020-09935-x","relation":{},"ISSN":["0282-0080","1573-4986"],"issn-type":[{"value":"0282-0080","type":"print"},{"value":"1573-4986","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,14]]},"assertion":[{"value":"23 May 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 June 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 July 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"all the authors consent to the publication of the manuscript.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The softwares used for: writing the paper (Microsoft Word), statistical analyses and graphs (GaphPad Prism 8), and for the figures formation (Microsoft PowerPoint) were licensed to the Host Institution: Department of Medical Biotechnology and Translational Medicine.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Code availability"}}]}}