{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T16:16:11Z","timestamp":1781712971092,"version":"3.54.5"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the General Projects of Medical and Health Science and Technology Plan in Zhejiang Province","award":["No. 2020KY035"],"award-info":[{"award-number":["No. 2020KY035"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Mol Histol"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Accumulating evidence has suggests that women with advanced endometriosis exhibit alterations in the expression of genes in the endometrium compared to healthy controls. Furthermore, replication stress is a characteristic feature of cancer cells, which results from sustained proliferative signaling induced by either the activation of oncogenes or the loss of tumor suppressors. In the present study, we propose that DNA replication ATP-dependent helicase\/nuclease 2 (DNA2) might be upregulated in endometriosis. Immunohistochemical staining results confirmed the hypothesis that DNA2 is overexpressed in the eutopic\/ectopic endometrium compared to that in a control endometrium from a healthy donor. Subsequently, ectopic endometrium-derived endometrial mesenchymal stem cells (EMSCs) showed the highest level of DNA2 and checkpoint kinase 1 (CHK1), as well as the strongest proliferation and migration capabilities, followed by eutopic endometrium-derived EMSCs, and then control EMSCs. To further analyze the function of DNA2, we knocked-down DNA2 expression in KLE cells. As expected, proliferation and migration declined when cells were transfected with DNA2 small interfering RNA. Taken together, our study demonstrated the overexpression of DNA2 in human endometriosis, which might be responsible for the upregulated cell proliferation and migration. This study provides insights into the mechanisms underlying human endometriosis.<\/jats:p>","DOI":"10.1007\/s10735-021-09983-z","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T09:11:28Z","timestamp":1622193088000},"page":"741-749","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Up-regulation of DNA2 results in cell proliferation and migration in endometriosis"],"prefix":"10.1007","volume":"52","author":[{"given":"Xinyan","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingya","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucheng","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0723-5876","authenticated-orcid":false,"given":"Yan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"9983_CR1","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1210\/me.2009-0468","volume":"24","author":"JA Aka","year":"2010","unstructured":"Aka JA, Mazumdar M, Chen CQ, Poirier D, Lin LSX (2010) 17\u03b2-Hydroxysteroid dehydrogenase type 1 stimulates breast cancer by dihydrotestosterone inactivation in addition to estradiol production. Mol Endocrinol 24:832\u2013845. https:\/\/doi.org\/10.1210\/me.2009-0468","journal-title":"Mol Endocrinol"},{"key":"9983_CR2","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.fertnstert","volume":"108","author":"CM Becker","year":"2017","unstructured":"Becker CM, Gattrell WT, Gude K, Singh SS (2017) Reevaluating response and failure of medical treatment of endometriosis: a systematic review. Fertil Steril 108:125\u2013136. https:\/\/doi.org\/10.1016\/j.fertnstert","journal-title":"Fertil Steril"},{"key":"9983_CR3","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1111\/j.1749-6632","volume":"955","author":"SE Bulun","year":"2002","unstructured":"Bulun SE, Yang S, Fang Z, Gurates B, Tamura M, Sebastian S (2002) Estrogen production and metabolism in endometriosis. Ann N Y Acad Sci 955:75\u201385. https:\/\/doi.org\/10.1111\/j.1749-6632","journal-title":"Ann N Y Acad Sci"},{"key":"9983_CR4","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/j.fertnstert","volume":"98","author":"RO Burney","year":"2012","unstructured":"Burney RO, Giudice LC (2012) Pathogenesis and pathophysiology of endometriosis. Fertil Steril 98:511\u2013519. https:\/\/doi.org\/10.1016\/j.fertnstert","journal-title":"Fertil Steril"},{"key":"9983_CR5","doi-asserted-by":"publisher","first-page":"3148","DOI":"10.1093\/humrep\/dem310","volume":"22","author":"H Dassen","year":"2007","unstructured":"Dassen H, Punyadeera C, Kamps R, Delvoux B, Van Langendonckt A, Donnez J, Husen B, Thole H, Dunselman G, Groothuis P (2007) Estrogen metabolizing enzymes in endometrium and endometriosis. Hum Reprod 22:3148\u20133158. https:\/\/doi.org\/10.1093\/humrep\/dem310","journal-title":"Hum Reprod"},{"key":"9983_CR6","doi-asserted-by":"publisher","first-page":"1020","DOI":"10.1016\/j.ajog","volume":"190","author":"L Fedele","year":"2004","unstructured":"Fedele L, Bianchi S, Zanconato G, Bettoni G, Gotsch F (2004) Long-term follow-up after conservative surgery for rectovaginal endometriosis. Am J Obstet Gynecol 190:1020\u20131024. https:\/\/doi.org\/10.1016\/j.ajog","journal-title":"Am J Obstet Gynecol"},{"key":"9983_CR7","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.1093\/humrep\/dev067","volume":"30","author":"DA Gibson","year":"2015","unstructured":"Gibson DA, Greaves E, Critchley HOD, Saunders PTK (2015) Estrogen-dependent regulation of human uterine natural killer cells promotes vascular remodelling via secretion of CCL2. Hum Reprod 30:1290\u20131301. https:\/\/doi.org\/10.1093\/humrep\/dev067","journal-title":"Hum Reprod"},{"key":"9983_CR8","doi-asserted-by":"publisher","first-page":"2389","DOI":"10.1056\/NEJMcp1000274","volume":"362","author":"LC Giudice","year":"2010","unstructured":"Giudice LC (2010) Endometriosis. N Engl J Med 362:2389\u20132398. https:\/\/doi.org\/10.1056\/NEJMcp1000274","journal-title":"N Engl J Med"},{"key":"9983_CR9","doi-asserted-by":"publisher","first-page":"960","DOI":"10.1016\/j.cell.2015.10.034","volume":"163","author":"SJ Han","year":"2015","unstructured":"Han SJ, Jung SY, Wu SP, Hawkins SM, Park MJ, Kyo S, Qin J, Lydon JP, Tsai SY, Tsai MJ (2015) Estrogen receptor \u03b2 modulates apoptosis complexes and the inflammasome to drive the pathogenesis of endometriosis. Cell 163:960\u2013974","journal-title":"Cell"},{"key":"9983_CR10","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1177\/1933719107309120","volume":"14","author":"G Hudelist","year":"2007","unstructured":"Hudelist G, Czerwenka K, Keckstein J, Haas C, Fink-Retter A, Gschwantler-Kaulich D, Kubista E, Singer CF (2007) Expression of aromatase and estrogen sulfotransferase in eutopic and ectopic endometrium: evidence for unbalanced estradiol production in endometriosis. Reprod Sci 14:798\u2013805. https:\/\/doi.org\/10.1177\/1933719107309120","journal-title":"Reprod Sci"},{"key":"9983_CR11","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/s0303-7207(00)00421-4","volume":"171","author":"B Husen","year":"2001","unstructured":"Husen B, Adamski J, Rune GM, Einspanier A (2001) Mechanisms of estradiol inactivation in primate endometrium. Mol Cell Endocrinol 171:179\u2013185. https:\/\/doi.org\/10.1016\/s0303-7207(00)00421-4","journal-title":"Mol Cell Endocrinol"},{"key":"9983_CR12","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1080\/14017430510035916","volume":"14","author":"J Keckstein","year":"2005","unstructured":"Keckstein J, Wiesinger H (2005) Deep endometriosis, including intestinal involvement\u2014the interdisciplinary approach. Minim Invasive Ther Allied Technol 14:160\u2013166. https:\/\/doi.org\/10.1080\/14017430510035916","journal-title":"Minim Invasive Ther Allied Technol"},{"key":"9983_CR13","doi-asserted-by":"publisher","first-page":"1080","DOI":"10.1093\/humrep\/14.4.1080","volume":"14","author":"K Kinkel","year":"1999","unstructured":"Kinkel K, Chapron C, Balleyguier C, Fritel X, Dubuisson JB, Moreau JF (1999) Magnetic resonance imaging characteristics of deep endometriosis. Hum Reprod 14:1080\u20131086. https:\/\/doi.org\/10.1093\/humrep\/14.4.1080","journal-title":"Hum Reprod"},{"key":"9983_CR14","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.fertnstert.2018.10.013","volume":"111","author":"PR Koninckx","year":"2019","unstructured":"Koninckx PR, Ussia A, Adamyan L, Wattiez A, Gomel V, Martin DC (2019) Pathogenesis of endometriosis: the genetic\/epigenetic theory. Fertil Steril 111:327\u2013340. https:\/\/doi.org\/10.1016\/j.fertnstert.2018.10.013","journal-title":"Fertil Steril"},{"key":"9983_CR15","first-page":"515","volume":"5","author":"C Kulkarni","year":"2016","unstructured":"Kulkarni C (2016) Study of endometriosis in women of reproductive age, laparoscopic management and its outcome. Int J Reprod Contracept Obstet Gynecol 5:515","journal-title":"Int J Reprod Contracept Obstet Gynecol"},{"key":"9983_CR16","doi-asserted-by":"publisher","first-page":"2783","DOI":"10.7150\/ijbs.35128","volume":"15","author":"ZZ Lai","year":"2019","unstructured":"Lai ZZ, Yang HL, Ha SY, Chang KK, Mei J, Zhou WJ, Qiu XM, Wang XQ, Zhu R, Li DJ (2019) Cyclooxygenase-2 in endometriosis. Int J Biol Sci 15:2783. https:\/\/doi.org\/10.7150\/ijbs.35128","journal-title":"Int J Biol Sci"},{"key":"9983_CR17","doi-asserted-by":"publisher","first-page":"3822","DOI":"10.3390\/ijms20153822","volume":"20","author":"RM Marquardt","year":"2019","unstructured":"Marquardt RM, Kim TH, Shin JH, Jeong JW (2019) Progesterone and estrogen signaling in the endometrium: what goes wrong in endometriosis? Int J Mol Sci 20:3822. https:\/\/doi.org\/10.3390\/ijms20153822","journal-title":"Int J Mol Sci"},{"key":"9983_CR18","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1067\/mob.2003.408","volume":"189","author":"RB Ness","year":"2003","unstructured":"Ness RB (2003) Endometriosis and ovarian cancer: thoughts on shared pathophysiology. Am J Obstet Gynecol 189:280\u2013294. https:\/\/doi.org\/10.1067\/mob.2003.408","journal-title":"Am J Obstet Gynecol"},{"key":"9983_CR19","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/j.fertnstert.2011.05.090","volume":"96","author":"Nnoaham KE, Hummelshoj L, Webster P, d\u2019Hooghe T, de Cicco Nardone F, de Cicco Nardone C, Jenkinson C, Kennedy SH, Zondervan KT, World Endometriosis Research Foundation Global Study","year":"2011","unstructured":"Nnoaham KE, Hummelshoj L, Webster P, d\u2019Hooghe T, de Cicco Nardone F, de Cicco Nardone C, Jenkinson C, Kennedy SH, Zondervan KT, World Endometriosis Research Foundation Global Study (2011) Impact of endometriosis on quality of life and work productivity: a multicenter study across ten countries. Fertil Steril 96:366-373.e8. https:\/\/doi.org\/10.1016\/j.fertnstert.2011.05.090","journal-title":"Fertil Steril"},{"key":"9983_CR20","doi-asserted-by":"publisher","first-page":"1562","DOI":"10.3390\/ijms18071562","volume":"18","author":"E Paw\u0142owska","year":"2017","unstructured":"Paw\u0142owska E, Szczepanska J, Blasiak J (2017) DNA2\u2014an important player in DNA damage response or just another DNA maintenance protein? Int J Mol Sci 18:1562. https:\/\/doi.org\/10.3390\/ijms18071562","journal-title":"Int J Mol Sci"},{"key":"9983_CR21","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1016\/s0015-0282(02)03329-0","volume":"78","author":"DB Redwine","year":"2002","unstructured":"Redwine DB (2002) Was Sampson wrong? Fertil Steril 78:686\u2013693. https:\/\/doi.org\/10.1016\/s0015-0282(02)03329-0","journal-title":"Fertil Steril"},{"key":"9983_CR22","doi-asserted-by":"publisher","first-page":"1893","DOI":"10.1002\/acn3.50888","volume":"6","author":"D Ronchi","year":"2019","unstructured":"Ronchi D, Liu C, Caporali L, Piga D, Li H, Tagliavini F, Valentino ML, Ferr\u00f2 MT, Bini P, Zheng L (2019) Novel mutations in DNA2 associated with myopathy and mtDNA instability. Ann Clin Transl Neurol 6:1893\u20131899. https:\/\/doi.org\/10.1002\/acn3.50888","journal-title":"Ann Clin Transl Neurol"},{"key":"9983_CR23","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1080\/15287390600974924","volume":"10","author":"D Roy","year":"2007","unstructured":"Roy D, Cai Q, Felty Q, Narayan S (2007) Estrogen-induced generation of reactive oxygen and nitrogen species, gene damage, and estrogen-dependent cancers. J Toxicol Environ Health B 10:235\u2013257. https:\/\/doi.org\/10.1080\/15287390600974924","journal-title":"J Toxicol Environ Health B"},{"key":"9983_CR24","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.canlet.2014.04.018","volume":"99","author":"RJ Santen","year":"2015","unstructured":"Santen RJ, Yue W, Wang JP (2015) Estrogen metabolites and breast cancer. Steroids 99:61\u201366. https:\/\/doi.org\/10.1016\/j.canlet.2014.04.018","journal-title":"Steroids"},{"key":"9983_CR25","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1016\/j.biocel.2010.03.008","volume":"42","author":"PG Signorile","year":"2010","unstructured":"Signorile PG, Baldi A (2010) Endometriosis: new concepts in the pathogenesis. Int J Biochem Cell Biol 42:778\u2013780. https:\/\/doi.org\/10.1016\/j.biocel.2010.03.008","journal-title":"Int J Biochem Cell Biol"},{"key":"9983_CR26","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.01.018","author":"DJ Skarzynski","year":"2020","unstructured":"Skarzynski DJ, Sz\u00f3stek-Mioduchowska AZ, Rebord\u00e3o MR, Jalali BM, Piotrowska-Tomala KK, Leciejewska N, \u0141azarczyk M, Ferreira-Dias GM (2020) Neutrophils, monocytes and other immune components in the equine endometrium: friends or foes? Theriogenology. https:\/\/doi.org\/10.1016\/j.theriogenology.2020.01.018","journal-title":"Theriogenology"},{"key":"9983_CR27","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/179515","author":"S Sourial","year":"2014","unstructured":"Sourial S, Tempest N, Hapangama DK (2014) Theories on the pathogenesis of endometriosis. Int J Reprod Med. https:\/\/doi.org\/10.1155\/2014\/179515","journal-title":"Int J Reprod Med"},{"key":"9983_CR28","doi-asserted-by":"publisher","first-page":"9396","DOI":"10.18632\/oncotarget.2414","volume":"5","author":"C Strauss","year":"2014","unstructured":"Strauss C, Kornowski M, Benvenisty A, Shahar A, Masury H, Ben-Porath I, Ravid T, Arbel-Eden A, Goldberg M (2014) The DNA2 nuclease\/helicase is an estrogen-dependent gene mutated in breast and ovarian cancers. Oncotarget 5:9396. https:\/\/doi.org\/10.18632\/oncotarget.2414","journal-title":"Oncotarget"},{"key":"9983_CR29","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1016\/j.fertnstert.2003.09.076","volume":"81","author":"M Tamura","year":"2004","unstructured":"Tamura M, Deb S, Sebastian S, Okamura K, Bulun SE (2004) Estrogen up-regulates cyclooxygenase-2 via estrogen receptor in human uterine microvascular endothelial cells. Fertil Steril 81:1351\u20131356. https:\/\/doi.org\/10.1016\/j.fertnstert.2003.09.076","journal-title":"Fertil Steril"},{"key":"9983_CR30","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.jpag.2011.06.012","volume":"24","author":"I Tandoi","year":"2011","unstructured":"Tandoi I, Somigliana E, Riparini J, Ronzoni S, Candiani M (2011) High rate of endometriosis recurrence in young women. J Pediatr Adolesc Gynecol 24:376\u2013379. https:\/\/doi.org\/10.1016\/j.jpag.2011.06.012","journal-title":"J Pediatr Adolesc Gynecol"},{"key":"9983_CR31","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.3390\/cells8101123","volume":"8","author":"ZR Tang","year":"2019","unstructured":"Tang ZR, Zhang R, Lian ZX, Deng SL, Yu K (2019) Estrogen-receptor expression and function in female reproductive disease. Cells 8:1123. https:\/\/doi.org\/10.3390\/cells8101123","journal-title":"Cells"},{"key":"9983_CR32","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1002\/mnfr.200500215","volume":"50","author":"J Wagner","year":"2006","unstructured":"Wagner J, Lehmann L (2006) Estrogens modulate the gene expression of Wnt-7a in cultured endometrial adenocarcinoma cells. Mol Nutr Food Res 50:368\u2013372. https:\/\/doi.org\/10.1002\/mnfr.200500215","journal-title":"Mol Nutr Food Res"},{"key":"9983_CR33","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1093\/molehr\/gaz040","volume":"25","author":"Z Xu","year":"2019","unstructured":"Xu Z, Zhang L, Yu Q, Zhang Y, Yan L, Chen ZJ (2019) The estrogen-regulated lncRNA H19\/miR-216a-5p axis alters stromal cell invasion and migration via ACTA2 in endometriosis. Mol Hum Reprod 25:550\u2013561. https:\/\/doi.org\/10.1093\/molehr\/gaz040","journal-title":"Mol Hum Reprod"},{"key":"9983_CR34","doi-asserted-by":"publisher","first-page":"4410","DOI":"10.3892\/mmr.2018.9428","volume":"18","author":"HL Yang","year":"2018","unstructured":"Yang HL, Chang KK, Mei J, Zhou WJ, Liu LB, Yao L, Meng Y, Wang MY, Ha SY, Lai ZZ (2018) Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion. Mol Med Rep 18:4410\u20134416. https:\/\/doi.org\/10.3892\/mmr.2018.9428","journal-title":"Mol Med Rep"},{"key":"9983_CR35","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1093\/humupd\/dmt024","volume":"19","author":"VJ Young","year":"2013","unstructured":"Young VJ, Brown JK, Saunders PT, Horne AW (2013) The role of the peritoneum in the pathogenesis of endometriosis. Hum Reprod Update 19:558\u2013569. https:\/\/doi.org\/10.1093\/humupd\/dmt024","journal-title":"Hum Reprod Update"},{"key":"9983_CR36","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1038\/s41586-018-0769-8","volume":"564","author":"Y Yu","year":"2018","unstructured":"Yu Y, Pham N, Xia B, Papusha A, Wang G, Yan Z, Peng G, Chen K, Ira G (2018) Dna2 nuclease deficiency results in large and complex DNA insertions at chromosomal breaks. Nature 564:287\u2013290. https:\/\/doi.org\/10.1038\/s41586-018-0769-8","journal-title":"Nature"},{"key":"9983_CR37","doi-asserted-by":"publisher","first-page":"4474","DOI":"10.1210\/jcem.83.12.5301","volume":"83","author":"K Zeitoun","year":"1998","unstructured":"Zeitoun K, Takayama K, Sasano H, Suzuki T, Moghrabi N, Andersson S, Johns A, Meng L, Putman M, Carr B (1998) Deficient 17\u03b2-hydroxysteroid dehydrogenase type 2 expression in endometriosis: failure to metabolize 17\u03b2-estradiol. J Clin Endocrinol Metab 83:4474\u20134480. https:\/\/doi.org\/10.1210\/jcem.83.12.5301","journal-title":"J Clin Endocrinol Metab"},{"key":"9983_CR38","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1093\/nar\/gkz1101","volume":"48","author":"L Zheng","year":"2020","unstructured":"Zheng L, Meng Y, Campbell JL, Shen B (2020) Multiple roles of DNA2 nuclease\/helicase in DNA metabolism, genome stability and human diseases. Nucleic Acids Res 48:16\u201335. https:\/\/doi.org\/10.1093\/nar\/gkz1101","journal-title":"Nucleic Acids Res"}],"container-title":["Journal of Molecular Histology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10735-021-09983-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10735-021-09983-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10735-021-09983-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T05:12:17Z","timestamp":1627621937000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10735-021-09983-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,28]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["9983"],"URL":"https:\/\/doi.org\/10.1007\/s10735-021-09983-z","relation":{},"ISSN":["1567-2379","1567-2387"],"issn-type":[{"value":"1567-2379","type":"print"},{"value":"1567-2387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,28]]},"assertion":[{"value":"25 December 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 May 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"We declare that there are no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was performed in accordance with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Zhejiang Provincial People\u2019s Hospital (November 9, 2019\/No. 2019KY204).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}