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Large amounts of CuONPs cause organ dysfunction in animals. Our study aim to compare between the toxic effects of CuONanSphere (CuONSp) and CuONanoFlower (CuONF) as new nano-pesticides, determine a less toxic form when used in agricultural applications. To characterize CuONSp and CuONF, we used X-ray diffraction (XRD), Field emission scanning electron microscopy (SEM), and High resolution transmission electron microscopy (HRTEM) and Zeta-sizer device.18 adult male albino rats were divided into three groups (n\u2009=\u20096), (I) control group, (II) and (III) groups were given orally 50\u00a0mg\/kg\/day of CuONSp and CuONF 30 days respectively. CuONSp induced oxidant-antioxidant abnormalities, including an increase in malondialdhyde (MDA) and a decrease in glutathione (GSH) in comparison to CuONF-treated one. CuONSp induced an increase in liver enzymes activities compared to CuONF. Tumour necrosis factor-alfa (TNF-\u03b1) detected an increased in liver and lung compared to CuONF. However, histological examinations revealed changes in CuONSp group than CuONF group. Changes in immune-expressions of TNF-\u03b1, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-k\u03b2) and tumour suppressor gene (p53) were also more identified in CuONSp group than CuONF group. Ultrastructural studies of liver and lung tissues marked alternations were observed in CuONSp group than CuONF group. In conclusion, CuONSp induced biological alternation in liver and lung more than CuONF. So, CuONF is less toxic compared to CuONSp when used as nano-pesticide in agricultural applications.<\/jats:p>\n                <jats:p><jats:bold>Graphical abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s10735-023-10115-y","type":"journal-article","created":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T11:41:54Z","timestamp":1680608514000},"page":"157-172","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative toxicological evaluations of novel forms nano-pesticides in liver and lung of albino rats"],"prefix":"10.1007","volume":"54","author":[{"given":"Abeer M.","family":"Abdel-Azeem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman S.","family":"Abdel-Rehiem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Farghali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma K.","family":"Khidr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0877-3097","authenticated-orcid":false,"given":"Manal","family":"Abdul-Hamid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"10115_CR1","doi-asserted-by":"publisher","first-page":"10812","DOI":"10.1038\/s41598-020-67784-y","volume":"10","author":"SA Abdelazeim","year":"2020","unstructured":"Abdelazeim SA, Shehata NI, Aly HF et al (2020) Amelioration of oxidative stressmediated apoptosis in copper oxide nanoparticlesinduced liver injury in rats by potent antioxidants. 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