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Although MCP exhibits notable immunomodulatory effects, its role in drug-induced splenic and systemic toxicity remains unexplored. This study investigated the effects of MCP modulation on splenic immune remodeling and galectin expression in a rat model of cisplatin-induced toxicity. Wistar rats were divided into four groups (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095 animals\/group): control (SHAM), MCP (100\u00a0mg\/kg\/day for 7 days), cisplatin (CIS, 10\u00a0mg\/kg\/day for 3 days), and MCP\u2009+\u2009CIS. Spleens were collected 6\u00a0h after the final cisplatin dose. Cisplatin induced splenic structural disorganization and selectively reduced nitric oxide levels without broadly affecting antioxidant enzymes. Cisplatin treatment was associated with increased CD3\u207a T cell labeling and enhanced tissue expression of Gal-1, -3, and \u2212\u20099. MCP administration did not restore splenic architecture but promoted increased hemosiderin deposition in the red pulp, suggestive of enhanced erythrophagocytosis and altered iron handling, and markedly reduced CD3\u207a T cell immunoreactivity. MCP associated with cisplatin also reduced Gal-1 and Gal-3 levels and altered the relationship between galectins and splenic immune cell populations. Correlation analyses revealed positive associations between Gal-1, -3, and \u2212\u20099 and CD68\u207a macrophages, as well as a selective association between Gal-3 and CD3\u207a T cells, exclusively in MCP\u2009+\u2009CIS animals. MCP does not mitigate cisplatin-induced splenic damage but alters immune cell distribution and galectin-related responses during cisplatin exposure.\n                  <\/jats:p>","DOI":"10.1007\/s10735-026-10828-w","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:33:35Z","timestamp":1778654015000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Modified citrus pectin modulates splenic immune responses and galectin expression following cisplatin treatment in Wistar rats"],"prefix":"10.1007","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1796-9577","authenticated-orcid":false,"given":"Diego","family":"Dias dos Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3182-7425","authenticated-orcid":false,"given":"Artur Francisco","family":"da Silva Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3015-6295","authenticated-orcid":false,"given":"Laura Santana","family":"de Chiara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6583-1329","authenticated-orcid":false,"given":"Mab Pereira","family":"Corr\u00eaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela Rodrigues","family":"da Silva-Sasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7025-7421","authenticated-orcid":false,"given":"Jos\u00e9 Marcos","family":"Sanches","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2956-6230","authenticated-orcid":false,"given":"Rinaldo","family":"Florencio-Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7078-6526","authenticated-orcid":false,"given":"Lila Missae","family":"Oyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6979-4126","authenticated-orcid":false,"given":"Cristiane Damas","family":"Gil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"key":"10828_CR1","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1038\/nri1669","volume":"5","author":"RE Mebius","year":"2005","unstructured":"Mebius RE, Kraal G (2005) Structure and function of the spleen. 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