{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T02:37:59Z","timestamp":1792895879727,"version":"4.5.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Izmir Katip Celebi University, Scientific Research and Project Coordinatorship","award":["2024-GAP-TIPF-0008"],"award-info":[{"award-number":["2024-GAP-TIPF-0008"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Mol Histol"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1007\/s10735-026-10896-y","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T07:17:51Z","timestamp":1784013471000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cell-free MSC secretome therapy ameliorates diabetic testicular injury via antioxidant mechanisms"],"prefix":"10.1007","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7854-4735","authenticated-orcid":false,"given":"Serbay","family":"Ozkan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7557-7611","authenticated-orcid":false,"given":"Basak","family":"Isildar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7899-280X","authenticated-orcid":false,"given":"Asl\u0131 Erdogan","family":"Oner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8664-3298","authenticated-orcid":false,"given":"Ismail","family":"Unal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2419-8587","authenticated-orcid":false,"given":"Ebru","family":"Emekli-Alturfan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2455-9605","authenticated-orcid":false,"given":"Meltem","family":"Kurus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0270-5069","authenticated-orcid":false,"given":"Meral","family":"Koyuturk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"10896_CR1","doi-asserted-by":"crossref","unstructured":"Atkinson MA, Eisenbarth GS, Michels AW (2014) Type 1 diabetes. Lancet","DOI":"10.1016\/S0140-6736(13)60591-7"},{"key":"10896_CR2","unstructured":"Bancroft J, Layton C, Suvarna S (2013) Bancroft\u2019s theory and practice of histological techniques content strategist. Michael Houston Content Development Specialists"},{"key":"10896_CR3","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28936","author":"S Barsiah","year":"2019","unstructured":"Barsiah S, Behnam-Rassouli M, Shahabipour F et al (2019) Evaluation of testis hormonal and histopathological alterations in type I and type II diabetic rats. J Cell Biochem. https:\/\/doi.org\/10.1002\/jcb.28936","journal-title":"J Cell Biochem"},{"key":"10896_CR4","doi-asserted-by":"crossref","unstructured":"Burrack AL, Martinov T, Fife BT (2017) T cell-mediated beta cell destruction: Autoimmunity and alloimmunity in the context of type 1 diabetes. Front Endocrinol (Lausanne).","DOI":"10.3389\/fendo.2017.00343"},{"key":"10896_CR5","doi-asserted-by":"publisher","first-page":"6651","DOI":"10.3390\/cimb45080420","volume":"45","author":"A Caturano","year":"2023","unstructured":"Caturano A, D\u2019Angelo M, Mormone A et al (2023) Oxidative Stress in Type 2 Diabetes: Impacts from Pathogenesis to Lifestyle Modifications. Curr Issues Mol Biol 45:6651\u20136666. https:\/\/doi.org\/10.3390\/cimb45080420","journal-title":"Curr Issues Mol Biol"},{"key":"10896_CR6","doi-asserted-by":"publisher","unstructured":"Chang H-H, Hsu S-P, Chien C-T (2019) Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy. Antioxid (Basel Switzerland) 9. https:\/\/doi.org\/10.3390\/antiox9010002","DOI":"10.3390\/antiox9010002"},{"key":"10896_CR7","doi-asserted-by":"crossref","unstructured":"Endlich K, Kliewe F, Endlich N (2017) Stressed podocytes\u2014mechanical forces, sensors, signaling and response. Pflugers Arch Eur J Physiol","DOI":"10.1007\/s00424-017-2025-8"},{"key":"10896_CR8","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1186\/s13287-023-03563-6","volume":"14","author":"J Faria","year":"2023","unstructured":"Faria J, Calcat-I-Cervera S, Skovronova R et al (2023) Mesenchymal stromal cells secretome restores bioenergetic and redox homeostasis in human proximal tubule cells after ischemic injury. Stem Cell Res Ther 14:353. https:\/\/doi.org\/10.1186\/s13287-023-03563-6","journal-title":"Stem Cell Res Ther"},{"key":"10896_CR9","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2014.03.004","author":"X Gao","year":"2014","unstructured":"Gao X, Song L, Shen K et al (2014) Bone marrow mesenchymal stem cells promote the repair of islets from diabetic mice through paracrine actions. Mol Cell Endocrinol. https:\/\/doi.org\/10.1016\/j.mce.2014.03.004","journal-title":"Mol Cell Endocrinol"},{"key":"10896_CR10","first-page":"453","volume":"57","author":"S Gawe\u0142","year":"2004","unstructured":"Gawe\u0142 S, Wardas M, Niedworok E, Wardas P (2004) [Malondialdehyde (MDA) as a lipid peroxidation marker]. Wiad Lek 57:453\u2013455","journal-title":"Wiad Lek"},{"key":"10896_CR11","doi-asserted-by":"publisher","first-page":"2503","DOI":"10.21873\/invivo.12531","volume":"35","author":"Z He","year":"2021","unstructured":"He Z, Yin G, Li QQ et al (2021) Diabetes mellitus causes male reproductive dysfunction: a review of the evidence and mechanisms. Vivo 35:2503\u20132511. https:\/\/doi.org\/10.21873\/invivo.12531","journal-title":"Vivo"},{"key":"10896_CR12","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/s41232-022-00241-7","volume":"42","author":"B Isildar","year":"2022","unstructured":"Isildar B, Ozkan S, Ercin M et al (2022) 2D and 3D cultured human umbilical cord-derived mesenchymal stem cell-conditioned medium has a dual effect in type 1 diabetes model in rats: immunomodulation and beta-cell regeneration. Inflamm Regen 42:55. https:\/\/doi.org\/10.1186\/s41232-022-00241-7","journal-title":"Inflamm Regen"},{"key":"10896_CR13","doi-asserted-by":"publisher","unstructured":"Isildar B, Ozkan S, Koyuturk M (2023) Therapeutic potential of mesenchymal stem cell-derived conditioned medium for diabetes mellitus and related complications. https:\/\/doi.org\/10.1002\/adtp.202300216. Adv Ther n\/a:2300216","DOI":"10.1002\/adtp.202300216"},{"key":"10896_CR15","doi-asserted-by":"publisher","first-page":"111662","DOI":"10.1016\/j.intimp.2024.111662","volume":"129","author":"B Isildar","year":"2024","unstructured":"Isildar B, Ozkan S, Sahin H et al (2024) Preconditioning of human umbilical cord mesenchymal stem cells with deferoxamine potentiates the capacity of the secretome released from the cells and promotes immunomodulation and beta cell regeneration in a rat model of type 1 diabetes. Int Immunopharmacol 129:111662. https:\/\/doi.org\/10.1016\/j.intimp.2024.111662","journal-title":"Int Immunopharmacol"},{"key":"10896_CR14","doi-asserted-by":"publisher","first-page":"177574","DOI":"10.1016\/j.ejphar.2025.177574","volume":"996","author":"B Isildar","year":"2025","unstructured":"Isildar B, Ozkan S, Neccar D, Koyuturk M (2025) Preconditioning and post-preconditioning states of mesenchymal stem cells with deferoxamine: a comprehensive analysis. Eur J Pharmacol 996:177574. https:\/\/doi.org\/10.1016\/j.ejphar.2025.177574","journal-title":"Eur J Pharmacol"},{"key":"10896_CR16","doi-asserted-by":"publisher","first-page":"3159","DOI":"10.3390\/ijms19103159","volume":"19","author":"F Kerans","year":"2018","unstructured":"Kerans F, Lungaro L, Azfer A, Salter D (2018) The Potential of intrinsically magnetic mesenchymal stem cells for tissue engineering. Int J Mol Sci 19:3159. https:\/\/doi.org\/10.3390\/ijms19103159","journal-title":"Int J Mol Sci"},{"key":"10896_CR17","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02173-4","author":"R Khatri","year":"2021","unstructured":"Khatri R, Petry SF, Linn T (2021) Intrapancreatic MSC transplantation facilitates pancreatic islet regeneration. Stem Cell Res Ther. https:\/\/doi.org\/10.1186\/s13287-021-02173-4","journal-title":"Stem Cell Res Ther"},{"key":"10896_CR18","doi-asserted-by":"publisher","first-page":"101071","DOI":"10.1016\/j.reth.2026.101071","volume":"31","author":"NE Lee","year":"2026","unstructured":"Lee NE, Hwang JI, Bang CY et al (2026) Wharton\u2019s jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1. Regen Ther 31:101071. https:\/\/doi.org\/10.1016\/j.reth.2026.101071","journal-title":"Regen Ther"},{"key":"10896_CR19","doi-asserted-by":"publisher","first-page":"8894","DOI":"10.1038\/srep08894","volume":"5","author":"Y Ma","year":"2015","unstructured":"Ma Y, Yang H-Z, Xu L-M et al (2015) Testosterone regulates the autophagic clearance of androgen binding protein in rat Sertoli cells. Sci Rep 5:8894. https:\/\/doi.org\/10.1038\/srep08894","journal-title":"Sci Rep"},{"key":"10896_CR20","doi-asserted-by":"publisher","first-page":"1613","DOI":"10.1007\/s11011-024-01393-w","volume":"39","author":"NE Mbiydzenyuy","year":"2024","unstructured":"Mbiydzenyuy NE, Qulu L-A (2024) Stress, hypothalamic-pituitary-adrenal axis, hypothalamic-pituitary-gonadal axis, and aggression. Metab Brain Dis 39:1613\u20131636. https:\/\/doi.org\/10.1007\/s11011-024-01393-w","journal-title":"Metab Brain Dis"},{"key":"10896_CR21","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1080\/01913123.2017.1397075","volume":"42","author":"EG Meydanli","year":"2018","unstructured":"Meydanli EG, Gumusel A, Ozkan S et al (2018) Effects of resveratrol on high-fructose-induced testis injury in rats. Ultrastruct Pathol 42:65\u201373. https:\/\/doi.org\/10.1080\/01913123.2017.1397075","journal-title":"Ultrastruct Pathol"},{"key":"10896_CR22","doi-asserted-by":"publisher","first-page":"34842","DOI":"10.1038\/srep34842","volume":"6","author":"K Nagaishi","year":"2016","unstructured":"Nagaishi K, Mizue Y, Chikenji T et al (2016) Mesenchymal stem cell therapy ameliorates diabetic nephropathy via the paracrine effect of renal trophic factors including exosomes. Sci Rep 6:34842. https:\/\/doi.org\/10.1038\/srep34842","journal-title":"Sci Rep"},{"key":"10896_CR23","doi-asserted-by":"publisher","first-page":"8484","DOI":"10.1038\/s41598-017-08921-y","volume":"7","author":"K Nagaishi","year":"2017","unstructured":"Nagaishi K, Mizue Y, Chikenji T et al (2017) Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy. Sci Rep 7:8484. https:\/\/doi.org\/10.1038\/s41598-017-08921-y","journal-title":"Sci Rep"},{"key":"10896_CR24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-13321-y","author":"SO Olojede","year":"2022","unstructured":"Olojede SO, Lawal SK, Faborode OS et al (2022) Testicular ultrastructure and hormonal changes following administration of tenofovir disoproxil fumarate-loaded silver nanoparticle in type-2 diabetic rats. Sci Rep. https:\/\/doi.org\/10.1038\/s41598-022-13321-y","journal-title":"Sci Rep"},{"key":"10896_CR25","doi-asserted-by":"publisher","first-page":"e0178011","DOI":"10.1371\/journal.pone.0178011","volume":"12","author":"C Oses","year":"2017","unstructured":"Oses C, Olivares B, Ezquer M et al (2017a) Preconditioning of adipose tissue-derived mesenchymal stem cells with deferoxamine increases the production of pro-angiogenic, neuroprotective and anti-inflammatory factors: Potential application in the treatment of diabetic neuropathy. PLoS ONE 12:e0178011. https:\/\/doi.org\/10.1371\/journal.pone.0178011","journal-title":"PLoS ONE"},{"key":"10896_CR26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178011","author":"C Oses","year":"2017","unstructured":"Oses C, Olivares B, Ezquer M et al (2017b) Preconditioning of adipose tissue-derived mesenchymal stem cells with deferoxamine increases the production of pro-angiogenic, neuroprotective and anti-inflammatory factors: Potential application in the treatment of diabetic neuropathy. PLoS ONE. https:\/\/doi.org\/10.1371\/journal.pone.0178011","journal-title":"PLoS ONE"},{"key":"10896_CR27","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1186\/s13287-022-03121-6","volume":"13","author":"S Ozkan","year":"2022","unstructured":"Ozkan S, Isildar B, Ercin M et al (2022) Therapeutic potential of conditioned medium obtained from deferoxamine preconditioned umbilical cord mesenchymal stem cells on diabetic nephropathy model. Stem Cell Res Ther 13:438. https:\/\/doi.org\/10.1186\/s13287-022-03121-6","journal-title":"Stem Cell Res Ther"},{"key":"10896_CR28","doi-asserted-by":"publisher","first-page":"122543","DOI":"10.1016\/j.lfs.2024.122543","volume":"343","author":"S Ozkan","year":"2024","unstructured":"Ozkan S, Isildar B, Sahin H et al (2024) Comparative analysis of effects of conditioned mediums obtained from 2D or 3D cultured mesenchymal stem cells on kidney functions of diabetic rats: Early intervention could potentiate transdifferentiation of parietal epithelial cell into podocyte precurso. Life Sci 343:122543. https:\/\/doi.org\/10.1016\/j.lfs.2024.122543","journal-title":"Life Sci"},{"key":"10896_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2019.01.003","author":"E \u00d6zta\u015f","year":"2019","unstructured":"\u00d6zta\u015f E, Y\u0131lmaz TE, G\u00fczel E et al (2019) Gliclazide alone or in combination with atorvastatin ameliorated reproductive damage in streptozotocin-induced type 2 diabetic male rats. Saudi Pharm J. https:\/\/doi.org\/10.1016\/j.jsps.2019.01.003","journal-title":"Saudi Pharm J"},{"key":"10896_CR30","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda-Carreras I, Frise E et al (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676\u2013682. https:\/\/doi.org\/10.1038\/nmeth.2019","journal-title":"Nat Methods"},{"key":"10896_CR31","doi-asserted-by":"publisher","first-page":"113880","DOI":"10.1016\/j.intimp.2024.113880","volume":"146","author":"J Sun","year":"2025","unstructured":"Sun J, Jia Y, Chen S et al (2025) Adipose mesenchymal stem cell-derived extracellular vesicles alleviate renal fibrosis by reducing epithelial-mesenchymal transition via the FOXS1\/Wnt\/\u03b2-catenin signaling pathway. Int Immunopharmacol 146:113880. https:\/\/doi.org\/10.1016\/j.intimp.2024.113880","journal-title":"Int Immunopharmacol"},{"key":"10896_CR32","unstructured":"Wu H, Mahato RI (2014) Mesenchymal stem cell-based therapy for type 1 diabetes. Discov Med"},{"key":"10896_CR33","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1007\/s00441-016-2499-2","volume":"368","author":"J Xv","year":"2017","unstructured":"Xv J, Ming Q, Wang X et al (2017) Mesenchymal stem cells moderate immune response of type 1 diabetes. Cell Tissue Res 368:239\u2013248. https:\/\/doi.org\/10.1007\/s00441-016-2499-2","journal-title":"Cell Tissue Res"},{"key":"10896_CR34","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/s40360-025-00847-w","volume":"26","author":"I Zangiabadi","year":"2025","unstructured":"Zangiabadi I, Askaripour M, Rajizadeh MA et al (2025) Conditioned medium from human adipose-derived mesenchymal stem cells attenuates cardiac injury induced by Movento in male rats: role of oxidative stress and inflammation. BMC Pharmacol Toxicol 26:13. https:\/\/doi.org\/10.1186\/s40360-025-00847-w","journal-title":"BMC Pharmacol Toxicol"}],"container-title":["Journal of Molecular Histology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10735-026-10896-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10735-026-10896-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10735-026-10896-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:49:24Z","timestamp":1786949364000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10735-026-10896-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,14]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10896"],"URL":"https:\/\/doi.org\/10.1007\/s10735-026-10896-y","relation":{},"ISSN":["1567-2379","1567-2387"],"issn-type":[{"value":"1567-2379","type":"print"},{"value":"1567-2387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,14]]},"assertion":[{"value":"16 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors have no competing interests to declare that are relevant to the content of this article.The authors declare no competing interests.","order":1,"name":"Ethics","label":"Conflict of Interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were conducted with the approval of the Experimental Animal Ethics Committee of Bezmi\u00e2lem Vak\u0131f University (approval no. E.106223; approved on 10 May 2023).","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"233"}}