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Interactions between Piezo1 and ZnT1-mediated zinc homeostasis and effects on barrier function during colitis were investigated in this study. Inflammation-induced barrier disruption was modeled in vivo using dextran sulfate-treated mice and in vitro using lipopolysaccharide-treated Caco-2 cells. Intestinal epithelial cell (IEC)-specific\n                    <jats:italic>Piezo1<\/jats:italic>\n                    knockout in mice, small interfering RNA-mediated silencing, Piezo1 agonist and antagonist, zinc ion (Zn\n                    <jats:sup>2+<\/jats:sup>\n                    ) supplement and chelator in Caco-2 cells were utilized to manipulate activities of Piezo1 and ZnT1. Suppression of Piezo1 in IECs alleviated inflammation-induced barrier damage\u00a0in vitro\u00a0and\u00a0in vivo. Bioinformatic analysis of UC patient datasets revealed an association between Piezo1 and zinc homeostasis and verified by elevated Piezo1 and ZnT1 expressions observed in both human UC and murine colitis samples. Suppression of Piezo1 led to ZnT1 downregulation in vitro\u00a0and\u00a0in vivo\n                    <jats:italic>.<\/jats:italic>\n                    Piezo1 activation caused enhanced Zn\n                    <jats:sup>2+<\/jats:sup>\n                    efflux and intracellular Zn\n                    <jats:sup>2+<\/jats:sup>\n                    depletion while Piezo1 inhibition led to Zn\n                    <jats:sup>2+<\/jats:sup>\n                    accumulation. Effects of zinc supplementation and chelation were consistent with the view that ZnT1 inhibition\u00a0alleviated\u00a0barrier disintegration. Our findings revealed that Piezo1 affected ZnT1-mediated zinc homeostasis in IECs. Piezo1 suppression downregulated ZnT1 expression, attenuating intracellular zinc depletion caused by excessive Zn\n                    <jats:sup>2+<\/jats:sup>\n                    efflux and relieved intestinal barrier disruption, indicating that Targeting the Piezo1-ZnT1-zinc signaling axis presents a promising therapeutic strategy for UC.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical Abstract<\/jats:bold>\n                  <\/jats:p>\n                  <jats:p>\n                    Piezo1-activation exacerbates inflammation-induced intestinal barrier disruption by triggering Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx which upregulates ZnT1-mediated Zn\n                    <jats:sup>2+<\/jats:sup>\n                    efflux resulting in IECs Zn\n                    <jats:sup>2+<\/jats:sup>\n                    dyshomeostasis, and this disruption can be rescued by Piezo1 suppression. The figure was generated by adobe illustrator.\n                  <\/jats:p>","DOI":"10.1007\/s10753-025-02448-5","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T07:55:36Z","timestamp":1769414136000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Piezo1 Regulates ZnT1-Mediated Zinc Homeostasis in Ulcerative Colitis"],"prefix":"10.1007","volume":"49","author":[{"given":"Weizhen","family":"Xiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyuan","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyao","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,26]]},"reference":[{"issue":"10","key":"2448_CR1","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1016\/j.tips.2024.08.003","volume":"45","author":"P Wangchuk","year":"2024","unstructured":"Wangchuk, P., K. 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