{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:41:28Z","timestamp":1784734888837,"version":"3.55.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2018,7,7]],"date-time":"2018-07-07T00:00:00Z","timestamp":1530921600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Int Ophthalmol"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1007\/s10792-018-0980-5","type":"journal-article","created":{"date-parts":[[2018,7,7]],"date-time":"2018-07-07T04:47:02Z","timestamp":1530938822000},"page":"1581-1587","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Genetic features of Pseudomonas aeruginosa isolates associated with eye infections referred to Farabi Hospital, Tehran, Iran"],"prefix":"10.1007","volume":"39","author":[{"given":"Farahnoosh","family":"Doustdar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Karimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zohreh","family":"Abedinyfar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahimeh Asadi","family":"Amoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Goudarzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,7]]},"reference":[{"key":"980_CR1","doi-asserted-by":"publisher","first-page":"4453","DOI":"10.1167\/iovs.06-0288","volume":"47","author":"MB Bandara","year":"2006","unstructured":"Bandara MB, Zhu H, Sankaridurg PR et al (2006) Salicylic acid reduces the production of several potential virulence factors of Pseudomonas aeruginosa associated with microbial keratitis. Investig Ophthalmol Vis Sci 47:4453\u20134460","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"980_CR2","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1080\/17469899.2016.1203254","volume":"11","author":"L Hazlett","year":"2016","unstructured":"Hazlett L, Suvas S, McClellan S et al (2016) Challenges of corneal infections. Expert Rev Ophthalmol 11:285\u2013297","journal-title":"Expert Rev Ophthalmol"},{"key":"980_CR3","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1099\/jmm.0.2008\/003723-0","volume":"57","author":"MH Choy","year":"2008","unstructured":"Choy MH, Stapleton F, Willcox MD et al (2008) Comparison of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens-and non-contact lens-related keratitis. J Med Microbiol 57:1539\u20131546","journal-title":"J Med Microbiol"},{"key":"980_CR4","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.clae.2016.01.004","volume":"39","author":"JJ Hoffman","year":"2016","unstructured":"Hoffman JJ, Pelosini L (2016) What happens when one leaves soft contact lenses in for three weeks? A case of bilateral, severe pseudomonas keratitis from contact lenses purchased over the internet. Contact Lens Anterior Eye 39:318\u2013320","journal-title":"Contact Lens Anterior Eye"},{"key":"980_CR5","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/j.exer.2016.01.013","volume":"145","author":"L Kautto","year":"2016","unstructured":"Kautto L, Nguyen-Khuong T, Everest-Dass A et al (2016) Glycan involvement in the adhesion of Pseudomonas aeruginosa to tears. Exp Eye Res 145:278\u2013288","journal-title":"Exp Eye Res"},{"key":"980_CR6","doi-asserted-by":"publisher","first-page":"e20130","DOI":"10.5812\/jjm.20130v2","volume":"8","author":"H Vaez","year":"2015","unstructured":"Vaez H, Faghri J, Esfahani BN et al (2015) Antibiotic resistance patterns and genetic diversity in clinical isolates of Pseudomonas aeruginosa isolated from patients of a referral hospital, Isfahan, Iran. Jundishapur J Microbiol 8:e20130","journal-title":"Jundishapur J Microbiol"},{"key":"980_CR7","first-page":"167","volume":"4","author":"A Yousefi-Avarvand","year":"2015","unstructured":"Yousefi-Avarvand A, Khashei R, Ebrahim-Saraie HS et al (2015) The frequency of exotoxin A and exoenzymes S and U genes among clinical isolates of Pseudomonas aeruginosa in Shiraz, Iran. Int J Mol Cell Med 4:167","journal-title":"Int J Mol Cell Med"},{"issue":"961\u2013970","key":"980_CR8","first-page":"e2","volume":"155","author":"DJ Evans","year":"2013","unstructured":"Evans DJ, Fleiszig SM (2013) Why does the healthy cornea resist Pseudomonas aeruginosa infection? Am J Ophthalmol 155(961\u2013970):e2","journal-title":"Am J Ophthalmol"},{"key":"980_CR9","doi-asserted-by":"publisher","first-page":"25","DOI":"10.18869\/modares.iem.2.1.25","volume":"2","author":"K Streeter","year":"2016","unstructured":"Streeter K, Katouli M (2016) Pseudomonas aeruginosa: a review of their pathogenesis and prevalence in clinical settings and the environment. Infect Epidemiol Med 2:25\u201332","journal-title":"Infect Epidemiol Med"},{"key":"980_CR10","doi-asserted-by":"publisher","first-page":"e86829","DOI":"10.1371\/journal.pone.0086829","volume":"9","author":"J Toska","year":"2014","unstructured":"Toska J, Sun Y, Carbonell DA et al (2014) Diversity of virulence phenotypes among type III secretion negative Pseudomonas aeruginosa clinical isolates. PLoS ONE 9:e86829","journal-title":"PLoS ONE"},{"key":"980_CR11","doi-asserted-by":"publisher","first-page":"6892","DOI":"10.1167\/iovs.15-17556","volume":"56","author":"N Oka","year":"2015","unstructured":"Oka N, Suzuki T, Ishikawa E et al (2015) Relationship of virulence factors and clinical features in keratitis caused by Pseudomonas aeruginosa virulence factors in P. aeruginosa keratitis. Investig Ophthalmol Vis Sci 56:6892\u20136898","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"980_CR12","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.micpath.2016.12.013","volume":"104","author":"S Lakhundi","year":"2017","unstructured":"Lakhundi S, Siddiqui R, Khan NA (2017) Pathogenesis of microbial keratitis. Microb Pathog 104:97\u2013109","journal-title":"Microb Pathog"},{"key":"980_CR13","doi-asserted-by":"publisher","first-page":"3892","DOI":"10.1167\/iovs.02-1302","volume":"44","author":"EJ Lee","year":"2003","unstructured":"Lee EJ, Cowell BA, Evans DJ et al (2003) Contribution of ExsA\u2013regulated factors to corneal infection by cytotoxic and invasive Pseudomonas aeruginosa in a murine scarification model. Investig Ophthalmol Vis Sci 44:3892\u20133898","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"980_CR14","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1186\/s12864-015-2069-0","volume":"16","author":"JJ Varga","year":"2015","unstructured":"Varga JJ, Barbier M, Mulet X et al (2015) Genotypic and phenotypic analyses of a Pseudomonas aeruginosa chronic bronchiectasis isolate reveal differences from cystic fibrosis and laboratory strains. BMC Genom 16:883","journal-title":"BMC Genom"},{"key":"980_CR15","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1080\/02713680500536746","volume":"31","author":"H Zhu","year":"2006","unstructured":"Zhu H, Conibear TC, Bandara R et al (2006) Type III secretion system\u2013associated toxins, proteases, serotypes, and antibiotic resistance of Pseudomonas aeruginosa isolates associated with keratitis. Curr Eye Res 31:297\u2013306","journal-title":"Curr Eye Res"},{"key":"980_CR16","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1016\/j.exer.2007.08.015","volume":"85","author":"C Tam","year":"2007","unstructured":"Tam C, Lewis S, Li W et al (2007) Mutation of the phospholipase catalytic domain of the Pseudomonas aeruginosa cytotoxin ExoU abolishes colonization promoting activity and reduces corneal disease severity. Exp Eye Res 85:799\u2013805","journal-title":"Exp Eye Res"},{"key":"980_CR17","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1167\/iovs.08-2358","volume":"50","author":"EC Ledbetter","year":"2009","unstructured":"Ledbetter EC, Mun JJ, Kowbel D et al (2009) Pathogenic phenotype and genotype of Pseudomonas aeruginosa isolates from spontaneous canine ocular infections. Investig Ophthalmol Vis Sci 50:729\u2013736","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"980_CR18","unstructured":"Wayne P (2007) Clinical and laboratory standards institute. Perform Stand Antimicrobial Susceptibility Test vol 17"},{"key":"980_CR19","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1097\/ICO.0b013e31815c5a3f","volume":"27","author":"A Pinna","year":"2008","unstructured":"Pinna A, Usai D, Sechi LA et al (2008) Detection of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens-associated corneal ulcers. Cornea 27:320\u2013326","journal-title":"Cornea"},{"key":"980_CR20","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1046\/j.1365-2958.1998.00797.x","volume":"28","author":"GA O\u2019toole","year":"1998","unstructured":"O\u2019toole GA, Kolter R (1998) Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol 28:449\u2013461","journal-title":"Mol Microbiol"},{"key":"980_CR21","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/j.jiac.2014.02.007","volume":"20","author":"S Yamaguchi","year":"2014","unstructured":"Yamaguchi S, Suzuki T, Kobayashi T et al (2014) Genotypic analysis of Pseudomonas aeruginosa isolated from ocular infection. J Infect Chemother 20:407\u2013411","journal-title":"J Infect Chemother"},{"key":"980_CR22","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1038\/nrmicro3422","volume":"13","author":"SJ Salter","year":"2015","unstructured":"Salter SJ (2015) Keeping an eye on P. aeruginosa. Nat Rev Microbiol 13:69","journal-title":"Nat Rev Microbiol"},{"key":"980_CR23","first-page":"47","volume-title":"Common diseases of the conjunctiva and cornea, common eye diseases and their management","author":"NR Galloway","year":"2016","unstructured":"Galloway NR, Amoaku WM, Galloway PH et al (2016) Common diseases of the conjunctiva and cornea, common eye diseases and their management. Springer, Berlin, pp 47\u201363"},{"key":"980_CR24","doi-asserted-by":"crossref","unstructured":"Hedayati H, Ghaderpanah M, Rasoulinejad SA et al (2015) Clinical presentation and antibiotic susceptibility of contact lens associated microbial keratitis. J Pathog 2015:152767","DOI":"10.1155\/2015\/152767"},{"key":"980_CR25","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.micpath.2014.11.014","volume":"78","author":"JL Priya","year":"2015","unstructured":"Priya JL, Prajna L, Mohankumar V (2015) Genotypic and phenotypic characterization of Pseudomonas aeruginosa isolates from post-cataract endophthalmitis patients. Microb Pathog 78:67\u201373","journal-title":"Microb Pathog"},{"key":"980_CR26","doi-asserted-by":"publisher","first-page":"3526","DOI":"10.1128\/JCM.41.8.3526-3531.2003","volume":"41","author":"T Ajayi","year":"2003","unstructured":"Ajayi T, Allmond LR, Sawa T et al (2003) Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system. J Clin Microbiol 41:3526\u20133531","journal-title":"J Clin Microbiol"},{"key":"980_CR27","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1038\/eye.2011.288","volume":"26","author":"F Stapleton","year":"2012","unstructured":"Stapleton F, Carnt N (2012) Contact lens-related microbial keratitis: how have epidemiology and genetics helped us with pathogenesis and prophylaxis. Eye 26:185\u2013193","journal-title":"Eye"},{"key":"980_CR28","first-page":"50","volume":"10","author":"N Jain","year":"2016","unstructured":"Jain N, Bhosale P, Tale V (2016) Biofilm formation on contact lenses by bacterial pathogens. J Pharm Res 10:50\u201353","journal-title":"J Pharm Res"},{"key":"980_CR29","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1099\/jmm.0.46005-0","volume":"54","author":"C Winstanley","year":"2005","unstructured":"Winstanley C, Kaye SB, Neal TJ et al (2005) Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis. J Med Microbiol 54:519\u2013526","journal-title":"J Med Microbiol"},{"key":"980_CR30","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1167\/iovs.14-15241","volume":"56","author":"EP Shen","year":"2015","unstructured":"Shen EP, Hsieh Y-T, Chu H-S et al (2015) Correlation of Pseudomonas aeruginosa genotype with antibiotic susceptibility and clinical features of induced central keratitis association of T3SS with pseudomonal keratitis. Investig Ophthalmol Vis Sci 56:365\u2013371","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"980_CR31","doi-asserted-by":"publisher","first-page":"6284","DOI":"10.1128\/IAI.69.10.6284-6295.2001","volume":"69","author":"JA Lomholt","year":"2001","unstructured":"Lomholt JA, Poulsen K, Kilian M (2001) Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors. Infect Immun 69:6284\u20136295","journal-title":"Infect Immun"},{"key":"980_CR32","doi-asserted-by":"publisher","first-page":"e64867","DOI":"10.1371\/journal.pone.0064867","volume":"8","author":"RS Karthikeyan","year":"2013","unstructured":"Karthikeyan RS, Priya JL, Leal SM Jr et al (2013) Host response and bacterial virulence factor expression in Pseudomonas aeruginosa and Streptococcus pneumoniae corneal ulcers. PLoS ONE 8:e64867","journal-title":"PLoS ONE"},{"key":"980_CR33","first-page":"e60116","volume":"10","author":"FB Bejestani","year":"2015","unstructured":"Bejestani FB, Hakemi-Vala M, Momtaheni R et al (2015) The frequency of imp and vim genes among pseudomonas aeruginosa isolates from children\u2019s medical center of tehran. Arch Clin Infect Dis 10:e60116","journal-title":"Arch Clin Infect Dis"},{"key":"980_CR34","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1111\/j.1469-0691.2007.01939.x","volume":"14","author":"A Wong-Beringer","year":"2008","unstructured":"Wong-Beringer A, Wiener-Kronish J, Lynch S et al (2008) Comparison of type III secretion system virulence among fluoroquinolone-susceptible and- resistant clinical isolates of Pseudomonas aeruginosa. Clin Microbiol Infect 14:330\u2013336","journal-title":"Clin Microbiol Infect"},{"key":"980_CR35","doi-asserted-by":"publisher","first-page":"111","DOI":"10.3390\/pathogens4010111","volume":"4","author":"PJ Bispo","year":"2015","unstructured":"Bispo PJ, Haas W, Gilmore MS (2015) Biofilms in infections of the eye. Pathogens 4:111\u2013136","journal-title":"Pathogens"},{"key":"980_CR36","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1167\/iovs.03-1050","volume":"45","author":"A Caballero","year":"2004","unstructured":"Caballero A, Thibodeaux B, Marquart M et al (2004) Pseudomonas keratitis: protease IV gene conservation, distribution, and production relative to virulence and other Pseudomonas proteases. Investig Ophthalmol Vis Sci 45:522\u2013530","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"980_CR37","doi-asserted-by":"publisher","first-page":"1831","DOI":"10.3390\/ijms18091831","volume":"18","author":"K Fukuda","year":"2017","unstructured":"Fukuda K, Ishida W, Fukushima A et al (2017) Corneal fibroblasts as sentinel cells and local immune modulators in infectious keratitis. Int J Mol Sci 18:1831","journal-title":"Int J Mol Sci"},{"key":"980_CR38","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1076\/0271-3683(200009)2131-RFT730","volume":"21","author":"C Pillar","year":"2000","unstructured":"Pillar C, Hazlett L, Hobden J (2000) Alkaline protease-deficient mutants of Pseudomonas aeruginosa are virulent in the eye. Curr Eye Res 21:730\u2013739","journal-title":"Curr Eye Res"},{"key":"980_CR39","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1128\/JCM.02036-10","volume":"49","author":"RM Stewart","year":"2011","unstructured":"Stewart RM, Wiehlmann L, Ashelford KE et al (2011) Genetic characterization indicates that a specific subpopulation of Pseudomonas aeruginosa is associated with keratitis infections. J Clin Microbiol 49:993\u20131003","journal-title":"J Clin Microbiol"}],"container-title":["International Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10792-018-0980-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10792-018-0980-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10792-018-0980-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,31]],"date-time":"2019-08-31T07:03:47Z","timestamp":1567235027000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10792-018-0980-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,7]]},"references-count":39,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["980"],"URL":"https:\/\/doi.org\/10.1007\/s10792-018-0980-5","relation":{},"ISSN":["0165-5701","1573-2630"],"issn-type":[{"value":"0165-5701","type":"print"},{"value":"1573-2630","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,7]]},"assertion":[{"value":"18 July 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 June 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 July 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors report no conflicts of interest and have no proprietary interest in any of the materials mentioned in this article.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}