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The presence of\n                      <jats:italic>icaAD<\/jats:italic>\n                      genes and\n                      <jats:italic>ica<\/jats:italic>\n                      operon was determined by the PCR assay. Phenotypic biofilm formation was verified using the microtitre plate assay. Antibiotic resistance was performed using the disc diffusion method. Staphylococcal ability to attach to host cells was assessed by flow cytometry. Cytotoxicity on epithelial cells was evaluated by LDH assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>ica<\/jats:italic>\n                      genes were detected in 26.9% of SE and in 42.3% of SA isolates. Only 15.3% of isolates (SE) were positive for both the\n                      <jats:italic>icaAD<\/jats:italic>\n                      and the\n                      <jats:italic>ica<\/jats:italic>\n                      operon. Phenotypically, 19.2% of SE isolates were strong biofilm producers, among which three were both\n                      <jats:italic>icaAD<\/jats:italic>\n                      - and\n                      <jats:italic>ica<\/jats:italic>\n                      operon-positive. About 26.9% of SA isolates were strong biofilm producers. Methicillin resistance (MR) was detected in 34.6% of SE and 26.9% of SA isolates. About 75% of MR isolates were multidrug resistant. SA isolates adhered to host cells more extensively than SE. SA isolates released higher level of LDH than SE.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Adherence abilities were commonly observed in staphylococci associated with conjunctivitis. However, low prevalence of isolates positive for a complete and functional\n                      <jats:italic>ica<\/jats:italic>\n                      locus and low prevalence of strong biofilm producers was detected. SA adhered to a greater extent to eukaryotic cells than SE and were more cytotoxic.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s10792-021-01818-w","type":"journal-article","created":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T18:02:35Z","timestamp":1616954555000},"page":"2585-2600","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the role of staphylococci in the pathomechanism of conjunctivitis"],"prefix":"10.1007","volume":"41","author":[{"given":"Ewa","family":"Jasi\u0144ska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Bogut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Magry\u015b","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina","family":"Olender","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,28]]},"reference":[{"issue":"8","key":"1818_CR1","doi-asserted-by":"publisher","first-page":"e0237603","DOI":"10.1371\/journal.pone.0237603","volume":"15","author":"HH DeCory","year":"2020","unstructured":"DeCory HH, Sanfilippo CM, Proskin HM, Blondeau JM (2020) Characterization of baseline polybacterial versus monobacterial infections in three randomized controlled bacterial conjunctivitis trials and microbial outcomes with besifloxacin ophthalmic suspension 0.6%. 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