{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:59:29Z","timestamp":1785517169876,"version":"3.56.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Centre of Excellence for Reproductive and Regenerative Medicine","award":["KK.01.1.1.01.0008"],"award-info":[{"award-number":["KK.01.1.1.01.0008"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Int Ophthalmol"],"DOI":"10.1007\/s10792-026-04193-6","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T08:19:03Z","timestamp":1785313143000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The impact of umbilical cord blood platelet lysate on human corneal endothelium during organ culture"],"prefix":"10.1007","volume":"46","author":[{"given":"Ivana","family":"Vidovi\u0107","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Himelreich-Peri\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branka","family":"Golubi\u0107 \u0106epuli\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirna","family":"Golemovi\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davor","family":"Je\u017eek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Kralik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Bojani\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"4193_CR1","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1136\/bjo.85.9.1075","volume":"85","author":"T M\u00f8ller-Pedersen","year":"2001","unstructured":"M\u00f8ller-Pedersen T, Hartmann U, M\u00f8ller H, Ehler N, Engelmann K (2001) Evaluation of potential organ culture media for eye banking using human corneas. Br J Ophthalmol 85:1075\u20131079. https:\/\/doi.org\/10.1136\/bjo.85.9.1075","journal-title":"Br J Ophthalmol"},{"issue":"3","key":"4193_CR2","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1034\/j.1600-0420.1999.770306.x","volume":"77","author":"H Ehlers","year":"1999","unstructured":"Ehlers H, Ehlers N, Hjortdal JO (1999) Corneal transplantation with donor tissue kept in organ culture for 7 weeks. Acta Ophthalmol Scand 77(3):277\u2013278. https:\/\/doi.org\/10.1034\/j.1600-0420.1999.770306.x","journal-title":"Acta Ophthalmol Scand"},{"issue":"1","key":"4193_CR3","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/s12929-024-01095-6","volume":"32","author":"RE Ghebosu","year":"2025","unstructured":"Ghebosu RE, Hui L, Wolfram J (2025) Increasing biomolecular relevance of cell culture practice. J Biomed Sci 32(1):3. https:\/\/doi.org\/10.1186\/s12929-024-01095-6","journal-title":"J Biomed Sci"},{"key":"4193_CR4","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.biomaterials.2015.10.065","volume":"76","author":"T Burnouf","year":"2016","unstructured":"Burnouf T, Strunk D, Koh M, Schallmoser K (2016) Human platelet lysate: replacing fetal bovine serum as a gold standard for human cell propagation? Biomaterials 76:371\u2013387","journal-title":"Biomaterials"},{"issue":"11","key":"4193_CR5","doi-asserted-by":"publisher","first-page":"6022","DOI":"10.3390\/ijms23116022","volume":"23","author":"PK Chaudhary","year":"2022","unstructured":"Chaudhary PK, Kim K, Kim S (2022) An insight into recent advances on platelet function in health and disease. Int J Mol Sci 23(11):6022. https:\/\/doi.org\/10.3390\/ijms23116022","journal-title":"Int J Mol Sci"},{"issue":"1","key":"4193_CR6","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.tibtech.2019.06.002","volume":"38","author":"K Schallmoser","year":"2020","unstructured":"Schallmoser K, Henschler R, Gabriel C, Koh MBC, Burnouf T (2020) Production and quality requirements of human platelet lysate: a position statement from the Working Party on Cellular Therapies of the International Society of Blood Transfusion. Trends Biotechnol 38(1):13\u201323. https:\/\/doi.org\/10.1016\/j.tibtech.2019.06.002","journal-title":"Trends Biotechnol"},{"key":"4193_CR7","doi-asserted-by":"publisher","DOI":"10.18332\/ejm\/192930","author":"A Bie\u0144","year":"2024","unstructured":"Bie\u0144 A, Vermeulen J, B\u0105czek B, Pi\u0119ta M, Pi\u0119ta B (2024) Cord blood banking: balancing hype and hope in stem cell therapy. Eur J Midwifery. https:\/\/doi.org\/10.18332\/ejm\/192930","journal-title":"Eur J Midwifery"},{"issue":"3","key":"4193_CR8","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1007\/s10561-024-10138-z","volume":"25","author":"Q Wang","year":"2024","unstructured":"Wang Q, Liu Y, Gao Y, Zhang M, Yang L, Hu J, Ren Y, Zhou Q, Zhu M (2024) Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C. Cell Tissue Bank 25(3):839\u2013850. https:\/\/doi.org\/10.1007\/s10561-024-10138-z","journal-title":"Cell Tissue Bank"},{"issue":"1","key":"4193_CR9","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3390\/bioengineering5010019","volume":"5","author":"I Christou","year":"2018","unstructured":"Christou I, Mallis P, Michalopoulos E, Chatzistamatiou T, Mermelekas G et al (2018) Evaluation of peripheral blood and cord blood platelet lysates in isolation and expansion of multipotent mesenchymal stromal cells. Bioengineering (Basel) 5(1):19. https:\/\/doi.org\/10.3390\/bioengineering5010019","journal-title":"Bioengineering (Basel)"},{"issue":"3","key":"4193_CR10","doi-asserted-by":"publisher","first-page":"208","DOI":"10.2450\/2020.0305-19","volume":"18","author":"D Samarkanova","year":"2020","unstructured":"Samarkanova D, Rodr\u00edguez L, Vives J, Coll R, Tahull E, Azqueta C et al (2020) Cord blood-derived platelet concentrates as starting material for new therapeutic blood components prepared in a public cord blood bank: from product development to clinical application. Blood Transfus 18(3):208\u2013216. https:\/\/doi.org\/10.2450\/2020.0305-19","journal-title":"Blood Transfus"},{"key":"4193_CR11","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1007\/s40123-020-00239-9","volume":"9","author":"G Giannaccare","year":"2020","unstructured":"Giannaccare G, Carnevali A, Senni C, Logozzo L, Scorcia V (2020) Umbilical cord blood and serum for the treatment of ocular diseases: a review. Ophthalmol Ther 9:235\u2013248. https:\/\/doi.org\/10.1007\/s40123-020-00239-9","journal-title":"Ophthalmol Ther"},{"issue":"1","key":"4193_CR12","doi-asserted-by":"publisher","first-page":"e2019021","DOI":"10.4084\/MJHID.2019.021","volume":"11","author":"CG Valentini","year":"2019","unstructured":"Valentini CG, Nuzzolo ER, Bianchi M, Orlando N, Iachininoto MG, Pinci P et al (2019) Cord blood platelet lysate: in vitro evaluation to support the use in regenerative medicine. Mediterr J Hematol Infect Dis 11(1):e2019021. https:\/\/doi.org\/10.4084\/MJHID.2019.021","journal-title":"Mediterr J Hematol Infect Dis"},{"issue":"2","key":"4193_CR13","doi-asserted-by":"publisher","first-page":"12","DOI":"10.3390\/medicines8020012","volume":"8","author":"A Tovar","year":"2021","unstructured":"Tovar A, White I, Sabater A (2021) Use of acellular umbilical cord-derived tissues in corneal and ocular surface diseases. Medicines (Basel) 8(2):12. https:\/\/doi.org\/10.3390\/medicines8020012","journal-title":"Medicines (Basel)"},{"key":"4193_CR14","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-48845-5","volume-title":"Light and specular microscopy of the cornea","author":"K Jirsova","year":"2017","unstructured":"Jirsova K (2017) Light and specular microscopy of the cornea. Springer, Cham. https:\/\/doi.org\/10.1007\/978-3-319-48845-5"},{"issue":"3","key":"4193_CR15","first-page":"245","volume":"63","author":"B Golubi\u0107 \u0106epuli\u0107","year":"2009","unstructured":"Golubi\u0107 \u0106epuli\u0107 B, Bojani\u0107 I, Mazi\u0107 S (2009) Banke krvi iz pupkovine. Acta Med Croatica 63(3):245\u2013250","journal-title":"Acta Med Croatica"},{"key":"4193_CR16","volume-title":"Guide to the preparation, use and quality assurance of blood components","author":"European Directorate for the Quality of Medicines & HealthCare (EDQM)","year":"2020","unstructured":"European Directorate for the Quality of Medicines & HealthCare (EDQM) (2020) Guide to the preparation, use and quality assurance of blood components, 5th ed. Council of Europe Publishing, Strasbourg","edition":"5th ed"},{"key":"4193_CR17","volume-title":"Technical manual","author":"ME Brecher","year":"2005","unstructured":"Brecher ME et al (2005) Technical manual, 15th edn. AABB, Bethesda (MD)","edition":"15"},{"issue":"2","key":"4193_CR18","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1167\/iovs.13-13386","volume":"55","author":"W Armitage","year":"2014","unstructured":"Armitage W, Jones M, Zambrano I, Carley F, Tole D (2014) The suitability of corneas stored by organ culture for penetrating keratoplasty and influence of donor and recipient factors on 5-year graft survival. Invest Ophthalmol Vis Sci 55(2):784\u2013791. https:\/\/doi.org\/10.1167\/iovs.13-13386","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"7","key":"4193_CR19","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1097\/01.ico.0000230253.62730.85","volume":"25","author":"N Builles","year":"2006","unstructured":"Builles N, Kodjikian L, Burillon C, Damour O (2006) Major endothelial loss from corneas in organ culture: importance of second endothelial count. Cornea 25(7):815\u2013820. https:\/\/doi.org\/10.1097\/01.ico.0000230253.62730.85","journal-title":"Cornea"},{"key":"4193_CR20","first-page":"1345","volume":"21","author":"F Forest","year":"2015","unstructured":"Forest F, Thuret G, Gain P, Dumollard J, Peoch M, Perrache C et al (2015) Optimization of immunostaining on flat-mounted human corneas. Mol Vis. 21:1345\u20131356","journal-title":"Mol Vis."},{"key":"4193_CR21","doi-asserted-by":"publisher","first-page":"29047","DOI":"10.1038\/srep29047","volume":"6","author":"Z He","year":"2016","unstructured":"He Z, Forest F, Gain P, Rageade D, Bernard A, Acquart S et al (2016) 3D map of the human corneal endothelial cell. Sci Rep 6:29047. https:\/\/doi.org\/10.1038\/srep29047","journal-title":"Sci Rep"},{"key":"4193_CR22","unstructured":"European Eye Bank Association (2020) Annual directory of the European Eye Bank Association, 28th ed.\u00a0"},{"key":"4193_CR23","doi-asserted-by":"publisher","first-page":"1496231","DOI":"10.3389\/ftox.2025.1496231","volume":"7","author":"S Immalaraju","year":"2025","unstructured":"Immalaraju S, Goyal S, Jonnalagadda R (2025) Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review. Front Toxicol 7:1496231. https:\/\/doi.org\/10.3389\/ftox.2025.1496231","journal-title":"Front Toxicol"},{"issue":"6","key":"4193_CR24","doi-asserted-by":"publisher","first-page":"712","DOI":"10.3109\/14653249.2010.548380","volume":"13","author":"L Huang","year":"2011","unstructured":"Huang L, Critser PJ, Grimes BR, Yoder MC (2011) Human umbilical cord blood plasma can replace fetal bovine serum for in vitro expansion of functional human endothelial colony forming cells. Cytotherapy 13(6):712\u2013721. https:\/\/doi.org\/10.3109\/14653249.2010.548380","journal-title":"Cytotherapy"},{"key":"4193_CR25","doi-asserted-by":"publisher","first-page":"108613","DOI":"10.1016\/j.exer.2021.108613","volume":"208","author":"C Petsoglou","year":"2021","unstructured":"Petsoglou C, Wen L, Hoque M, Zhu M, Valtink M, Sutton G, You J (2021) Effects of human platelet lysate on the growth of cultured human corneal endothelial cells. Exp Eye Res 208:108613. https:\/\/doi.org\/10.1016\/j.exer.2021.108613","journal-title":"Exp Eye Res"},{"issue":"8","key":"4193_CR26","first-page":"1757","volume":"42","author":"J Crewe","year":"2001","unstructured":"Crewe J, Armitage W (2001) Integrity of epithelium and endothelium in organ cultured human corneas. Invest Ophthalmol Vis Sci 42(8):1757\u20131761","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"4","key":"4193_CR27","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1007\/s10561-021-09918-8","volume":"22","author":"Z He","year":"2021","unstructured":"He Z, Okumura N, Sato M, Komori Y, Nakahara M, Gain P, Koizumi K, Thuret G (2021) Corneal endothelial cell therapy: feasibility of cell culture from corneas stored in organ culture. Cell Tissue Bank 22(4):551\u2013562. https:\/\/doi.org\/10.1007\/s10561-021-09918-8","journal-title":"Cell Tissue Bank"},{"issue":"4","key":"4193_CR28","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1111\/j.1755-3768.2008.01490.x","volume":"88","author":"J Nejepinska","year":"2010","unstructured":"Nejepinska J, Juklova K, Jirsova K (2010) Organ culture, but not hypothermic storage, facilitates the repair of the corneal endothelium following mechanical damage. Acta Ophthalmol 88(4):413\u2013419. https:\/\/doi.org\/10.1111\/j.1755-3768.2008.01490.x","journal-title":"Acta Ophthalmol"},{"issue":"3","key":"4193_CR29","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1136\/bjo.86.3.306","volume":"86","author":"P Gain","year":"2002","unstructured":"Gain P (2002) Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique. Br J Ophthalmol 86(3):306\u2013310. https:\/\/doi.org\/10.1136\/bjo.86.3.306","journal-title":"Br J Ophthalmol"},{"key":"4193_CR30","first-page":"3494","volume":"17","author":"Z He","year":"2011","unstructured":"He Z, Campolmi N, Ha Thi B, Dumollard J, Peoc\u2019h M, Garraud O et al (2011) Immunolocalization of cell cycle proteins in corneal endothelial cells. Mol Vis. 17:3494\u20133511","journal-title":"Mol Vis."},{"key":"4193_CR31","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1186\/s13287-021-02611-3","volume":"12","author":"I Smeringaiova","year":"2021","unstructured":"Smeringaiova I, Utheim TP, Jirsova K (2021) Ex vivo expansion and characterization of human corneal endothelium for transplantation: a review. Stem Cell Res Ther 12:554. https:\/\/doi.org\/10.1186\/s13287-021-02611-3","journal-title":"Stem Cell Res Ther"},{"issue":"3","key":"4193_CR32","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1034\/j.1600-0420.1999.770304.x","volume":"77","author":"C Redbrake","year":"1999","unstructured":"Redbrake C, Salla S, Frantz A, Reim M (1999) Metabolic changes of the human donor cornea during organ culture. Acta Ophthalmol 77(3):266\u2013272. https:\/\/doi.org\/10.1034\/j.1600-0420.1999.770304.x","journal-title":"Acta Ophthalmol"},{"key":"4193_CR33","doi-asserted-by":"publisher","first-page":"108193","DOI":"10.1016\/j.exer.2020.108193","volume":"199","author":"S Li","year":"2020","unstructured":"Li S, Shyam R, Ogando DG, Bonanno JA (2020) Bicarbonate activates glycolysis and lactate production in corneal endothelial cells by increased pHi. Exp Eye Res 199:108193. https:\/\/doi.org\/10.1016\/j.exer.2020.108193","journal-title":"Exp Eye Res"},{"issue":"4","key":"4193_CR34","doi-asserted-by":"publisher","first-page":"E239","DOI":"10.1097\/OPX.0b013e3181d39464","volume":"87","author":"SP Srinivas","year":"2010","unstructured":"Srinivas SP (2010) Dynamic regulation of barrier integrity of the corneal endothelium. Optom Vis Sci 87(4):E239\u2013E254","journal-title":"Optom Vis Sci"},{"key":"4193_CR35","doi-asserted-by":"publisher","first-page":"108068","DOI":"10.1016\/j.exer.2020.108068","volume":"198","author":"SD Klyce","year":"2020","unstructured":"Klyce SD (2020) Endothelial pump and barrier function. Exp Eye Res 198:108068. https:\/\/doi.org\/10.1016\/j.exer.2020.108068","journal-title":"Exp Eye Res"},{"key":"4193_CR36","doi-asserted-by":"publisher","first-page":"10227","DOI":"10.3390\/ijms251810227","volume":"25","author":"P Anney","year":"2024","unstructured":"Anney P, Charpentier P, Proulx P (2024) Influence of intraocular pressure on the expression and activity of sodium-potassium pumps in the corneal endothelium. Int J Mol Sci 25:10227. https:\/\/doi.org\/10.3390\/ijms251810227","journal-title":"Int J Mol Sci"},{"key":"4193_CR37","doi-asserted-by":"publisher","first-page":"2695212","DOI":"10.1155\/2022\/2695212","volume":"2022","author":"W Lin","year":"2022","unstructured":"Lin W, Chen M, Cisse Y, Chen X, Bai L (2022) Roles and mechanisms of regulated necrosis in corneal diseases: progress and perspectives. J Ophthalmol 2022:2695212. https:\/\/doi.org\/10.1155\/2022\/2695212","journal-title":"J Ophthalmol"},{"issue":"10","key":"4193_CR38","first-page":"2887","volume":"41","author":"J Albon","year":"2000","unstructured":"Albon J, Tullo AB, Aktar S, Boulton ME (2000) Apoptosis in the endothelium of human corneas for transplantation. Invest Ophthalmol Vis Sci 41(10):2887\u20132893","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"4193_CR39","doi-asserted-by":"publisher","first-page":"73","DOI":"10.2450\/2015.0122-15","volume":"14","author":"P Rebulla","year":"2016","unstructured":"Rebulla P, Pupella S, Santodirocco M, Greppi N, Villanova I, Buzzi M et al (2016) Multicentric standardisation of the clinical grade procedure for the preparation of allogeneic platelet concentrates from umbilical cord blood. Blood Transfus 14(1):73\u201379. https:\/\/doi.org\/10.2450\/2015.0122-15","journal-title":"Blood Transfus"}],"container-title":["International Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10792-026-04193-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10792-026-04193-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10792-026-04193-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:05:08Z","timestamp":1785513908000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10792-026-04193-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["4193"],"URL":"https:\/\/doi.org\/10.1007\/s10792-026-04193-6","relation":{},"ISSN":["1573-2630"],"issn-type":[{"value":"1573-2630","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]},"assertion":[{"value":"15 April 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper. All authors certify that they have no affiliations with or involvement in any organization or entity with any financial or non-financial interest in the subject matter or materials discussed in this manuscript. The authors declare that they have no competing interests.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Institutional Review Board of the University Hospital Centre Zagreb approved the protocol of this study, and it conformed to the tenets of the Declaration of Helsinki (02\/21 AG from 20th December 2021).","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Written informed consent was obtained from all the donor corneas that were included in this study.","order":3,"name":"Ethics","label":"Informed consent","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Parts of this work were previously presented in abstract form at the 9th Croatian Transfusion Congress (Lovran, Croatia, 2025), the 32nd Annual Congress of the European Association of Tissue and Cell Banks (Barcelona, Spain, 2024), the European Eye Bank Association Meeting (Antwerp, Belgium, 2024), and the Annual Congress of the European Association of Tissue and Cell Banking (Zagreb, Croatia, 2023). The present manuscript substantially expands the experimental data, analysis, and interpretation and has not been previously published as a full-length article.","order":4,"name":"Ethics","label":"Previous presentation","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"316"}}