{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T11:06:14Z","timestamp":1776078374909,"version":"3.50.1"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T00:00:00Z","timestamp":1166572800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Assist Reprod Genet"],"published-print":{"date-parts":[[2007,2,2]]},"DOI":"10.1007\/s10815-006-9079-4","type":"journal-article","created":{"date-parts":[[2006,12,19]],"date-time":"2006-12-19T20:11:50Z","timestamp":1166559110000},"page":"47-51","source":"Crossref","is-referenced-by-count":40,"title":["Seven pregnancies and deliveries from non-mosaic Klinefelter syndrome patients using fresh and frozen testicular sperm"],"prefix":"10.1007","volume":"24","author":[{"given":"Koichi","family":"Kyono","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirofumi","family":"Uto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Nakajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shima","family":"Kumagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhisa","family":"Araki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Kanto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,12,20]]},"reference":[{"key":"9079_CR1","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1093\/humrep\/14.4.946","volume":"14","author":"H Okada","year":"1999","unstructured":"Okada H, Fujioka H, Tatsumi N, Kanzaki M, Okuda Y, Fujisawa M, Hazama M, Matsumoto O, Gohji K, Arakawa S, Kamidono S. Klinefelter syndrome in the male infertility clinic. Hum Reprod 1999;14:946\u201352.","journal-title":"Hum Reprod"},{"key":"9079_CR2","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/S0015-0282(01)02830-8","volume":"76","author":"V Poulakis","year":"2001","unstructured":"Poulakis V, Witzsch U, Diehl W, de Vries R, Becht E, Trotnow S. Birth of two infants with normal karyotype after intracytoplasmic injection of sperm obtained by testicular extraction from two men with non-mosaic Klinefelter\u2019s syndrome. Fertil Steril 2001;76:1060\u20132.","journal-title":"Fertil Steril"},{"key":"9079_CR3","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.1093\/humrep\/16.11.2347","volume":"16","author":"K Kyono","year":"2001","unstructured":"Kyono K, Fukunaga N, Haigo K, Chiba S, Araki Y. Pregnancy achieved following ICSI from a man with Klinefelter\u2019s syndrome and spinal cord injury. Hum Reprod 2001;16:2347\u20139.","journal-title":"Hum Reprod"},{"key":"9079_CR4","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1093\/humrep\/15.8.1804","volume":"15","author":"R Ron-EI","year":"2000","unstructured":"Ron-EI R, Strassburger D, Gelman-Kohan S, Friedler S, Raziel A, Appleman Z. A 47, XXY fetus conceived after ICSI of spermatozoa from a patient with non-mosaic Klinefelter\u2019s syndrome. Hum Reprod 2000a;15:1804\u20136.","journal-title":"Hum Reprod"},{"key":"9079_CR5","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1056\/NEJM199802263380905","volume":"338","author":"GP Palermo","year":"1998","unstructured":"Palermo GP, Schlegel PN, Sills ES, Veeck L, Zaninovic N, Menendez S, Rosenwaks Z. Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with non-mosaic Klinefelter\u2019s syndrome. N Engl J Med 1998;338:588\u20139.","journal-title":"N Engl J Med"},{"key":"9079_CR6","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/S0015-0282(99)00151-X","volume":"71","author":"F Nodar","year":"1999","unstructured":"Nodar F, De Vincentiis S, Olmed SB, Papier S, Urrutia F, Acosta AA. Birth of twin males with normal karyotype after intracytoplasmic sperm injection with use of testicular spermatozoa from a non-mosaic patient with Klinefelter\u2019s syndrome. Fertil Steril 1999;71:1149\u201352.","journal-title":"Fertil Steril"},{"key":"9079_CR7","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/S0015-0282(00)01556-9","volume":"74","author":"J Levron","year":"2000","unstructured":"Levron J, Aviram-Goldring A, Madger L, Raviv, G, Barkai G, Dor J. Sperm chromosome analysis and outcome of IVF in patients with non-mosaic Klinefelter\u2019s syndrome. Fertil Steril 2000;74:925\u20139.","journal-title":"Fertil Steril"},{"key":"9079_CR8","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1093\/humrep\/16.12.2616","volume":"16","author":"S Friedler","year":"2001","unstructured":"Friedler S, Raziel A, Strassburger D, Schachter M, Bern O, Ron-EI R. Outcome of ICSI using fresh and cryopreserved-thawed testicular spermatozoa in patients with non-mosaic Klinefelter\u2019s syndrome. Hum Reprod 2001;16:2616\u201320.","journal-title":"Hum Reprod"},{"key":"9079_CR9","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1016\/S0015-0282(00)00710-X","volume":"74","author":"R Ron-EI","year":"2000","unstructured":"Ron-EI R, Razziel A, Strassburger D, Schachter M, Bern O, Friedler S. Birth of healthy male twins after intracytoplasmic sperm injection of frozen-thawed testicular spermatozoa from a patient with non-mosaic Klinefelter\u2019s syndrome. Fertil Steril 2000b;74:832\u20133.","journal-title":"Fertil Steril"},{"key":"9079_CR10","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.fertnstert.2005.05.053","volume":"84","author":"H Okada","year":"2005","unstructured":"Okada H, Goda K, Muto S, Maruyama O, Koshida M, Horie S. Four pregnancies in non-mosaic Klinefelter\u2019s syndrome using cryopreserved-thawed testicular spermatozoa. Fertil Steril 2005;84:13\u20136.","journal-title":"Fertil Steril"},{"key":"9079_CR11","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1023\/A:1014792922874","volume":"19","author":"B Rosenlund","year":"2002","unstructured":"Rosenlund B, Hreinsson JG, Hovatta O. Birth of a healthy male after frozen thawed blastocyst transfer following intracytoplasmic injection of frozen thawed testicular spermatozoa from a man with nonmosaic Klinefelter\u2019s syndrome. J Assist Reprod Genet 2002;19:149\u201351.","journal-title":"J Assist Reprod Genet"},{"key":"9079_CR12","first-page":"121","volume-title":"Preembryo grading. Atlas of the Human Oocyte and Early Conceptus. Vol. II","author":"LL Veeck","year":"1991","unstructured":"Veeck LL. Preembryo grading. Atlas of the Human Oocyte and Early Conceptus. Vol. II. Baltimore: Williams & Wilkins; 1991. pp. 121\u201349."},{"key":"9079_CR13","first-page":"378","volume-title":"Towards reproductive certainty: Fertility & genetics beyond","author":"DK Gardner","year":"1999","unstructured":"Gardner DK, Schoolcraft WB. In vitro culture of human blastocysts. In: Jansen R, Mortimer D, editors. Towards reproductive certainty: Fertility & genetics beyond. Sydney: Parthenon Publishing; 1999. pp. 378\u201388."},{"key":"9079_CR14","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s10815-006-9036-2","volume":"23","author":"K Kyono","year":"2006","unstructured":"Kyono K, Nakajo Y, Kumagai S, Sasaki S. Efficacy of low dose human chorionic gonadotropin (hCG) in a GnRH antagonist protocol. J Assist Reprod Genet 2006;23:223\u20138.","journal-title":"J Assist Reprod Genet"},{"key":"9079_CR15","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1023\/A:1013107132110","volume":"18","author":"N Fukunaga","year":"2001","unstructured":"Fukunaga N, Haigo K, Kyono K, Araki Y. Efficiency of using frozen-thawed testicular sperm for multiple intracytoplasmic sperm injections. J Assist Reprod Genet 2001;18:634\u20137.","journal-title":"J Assist Reprod Genet"},{"key":"9079_CR16","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1093\/humrep\/12.5.994","volume":"12","author":"J Cohen","year":"1997","unstructured":"Cohen J, Garris GJ, Congedo-Ferrara TA, Kieck KA, Schimmel TW, Scott RR. Cryopreservation of single human spermatozoa. Hum Reprod 1997;12:994\u20131001.","journal-title":"Hum Reprod"},{"key":"9079_CR17","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1093\/humrep\/deg162","volume":"18","author":"TG Schuster","year":"2003","unstructured":"Schuster TG, Keller LM, Dunn RL, Ohl DA, Smith GD. Ultra-rapid freezing of very low numbers of sperm using cryoloops. Hum Reprod 2003;18:788\u201395.","journal-title":"Hum Reprod"},{"key":"9079_CR18","unstructured":"Kyono K. unpublished data"},{"key":"9079_CR19","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/S0015-0282(02)03092-3","volume":"77","author":"I Madgar","year":"2002","unstructured":"Madgar I, Dor J, Weissenberg R, Radiv G, Menashe Y, Levron J. Prognostic value of a clinical and laboratory evaluationin patients with nonmosaic Klinefelter syndrome who are receiving assisted reproductive therapy. Fertil Steril 2002;77:1167\u20139.","journal-title":"Fertil Steril"},{"key":"9079_CR20","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/S0015-0282(01)01793-9","volume":"75","author":"G Westlander","year":"2001","unstructured":"Westlander G, Ekerhovd E, Granberg S, Hanson L, Hanson C, Berrgh C. Testicular ultrasonography and extended chromosome analysis in men with nonmosaic Klinefelter syndrome: a prospective study of possible predictive factors for successful sperm recovery. Fertil Steril 2001;75:1102\u20135.","journal-title":"Fertil Steril"},{"key":"9079_CR21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1093\/humrep\/14.1.131","volume":"14","author":"PN Schlegel","year":"1999","unstructured":"Schlegel PN. Testicular sperm extraction: microdissection improves sperm yield with minimal tissue excision. Hum Reprod 1999;14:131\u20135.","journal-title":"Hum Reprod"},{"key":"9079_CR22","first-page":"6264","volume":"90","author":"JD Schiff","year":"2005","unstructured":"Schiff JD, Palermo GD, Veeck LL, Goldstein M, Rosenwaks Z, Schlegel PN. Success of testicular sperm injection and intracytoplasmic sperm injection in men with Klinefelter syndrome. J Clin Endocrinol Meatb 2005;90:6264\u20137.","journal-title":"J Clin Endocrinol Meatb"},{"key":"9079_CR23","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/S0022-5347(01)69099-2","volume":"167","author":"JD Raman","year":"2002","unstructured":"Raman JD, Schlegel PN. Aromatase inhibitors for male infertility. J Urol 2002;167:624\u20139.","journal-title":"J Urol"},{"issue":"Suppl.","key":"9079_CR24","doi-asserted-by":"crossref","first-page":"S206","DOI":"10.1016\/j.fertnstert.2005.07.521","volume":"84","author":"H Okada","year":"2005","unstructured":"Okada H, Maruyama O, Muto S, Yamamoto Y, Koshida M, Horie S. Age as a limiting factor for successful sperm retrieval in nonmosaic Klinefelter syndrome. Fertil Steril 2005;84(Suppl.):S206.","journal-title":"Fertil Steril"},{"key":"9079_CR25","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1093\/humrep\/12.1.80","volume":"12","author":"H Tournaye","year":"1997","unstructured":"Tournaye H, Verheyen G, Nagy P, Goossens A, Ubaldi F, Silber S, Van Steirteghem A, Devroey P. Are there any predictive factors for successful testicular sperm recovery in azoospermic patients? Hum Reprod 1997;12:80\u20136.","journal-title":"Hum Reprod"},{"key":"9079_CR26","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1093\/humrep\/deh253","volume":"19","author":"V Vernaeve","year":"2004","unstructured":"Vernaeve V, Staessen C, Verheyen G, Van Steirteghem A, Devroey P, Tournaye H. Can biological or clinical parameters predict testicular sperm recovery in 47, XXY Klinefelter\u2019s syndrome patients? Hum Reprod 2004;19:1135\u20139.","journal-title":"Hum Reprod"},{"issue":"1017e","key":"9079_CR27","first-page":"5","volume":"84","author":"K Kyono","year":"2005","unstructured":"Kyono K, Fuchinoue K, Yagi A, Nakajo Y, Yamashita A, Kumagai S. Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte. Fertil Steril 2005;84(1017e):5\u20136.","journal-title":"Fertil Steril"},{"key":"9079_CR28","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0140-6736(04)16678-6","volume":"364","author":"F Lanfranco","year":"2004","unstructured":"Lanfranco F, Kamischke A, Zitzmann M, Nieschlag E. Klinefelter\u2019s syndrome. Lancet 2004;364:273\u201383.","journal-title":"Lancet"},{"key":"9079_CR29","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.fertnstert.2003.09.085","volume":"82","author":"D Denschlag","year":"2004","unstructured":"Denschlag D, Tempfer C, Kunze M, Wolff G, Keck C. Assisted reproductive techniques in patients with Klinefelter syndrome: a critical review. Fertil Steril 2004;82:775\u20139.","journal-title":"Fertil Steril"},{"key":"9079_CR30","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1093\/humupd\/dmg029","volume":"9","author":"C Staessen","year":"2003","unstructured":"Staessen C, Tournaye H, Van Assche E, Michiels A, Van Landuyt L, Devroey P, Liebaers I, Van Steirteghem A. PGD in 47, XXY Klinefelter\u2019s syndrome patients. Hum Reprod Update 2003;9:319\u201330.","journal-title":"Hum Reprod Update"}],"container-title":["Journal of Assisted Reproduction and Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-006-9079-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10815-006-9079-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-006-9079-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T05:36:35Z","timestamp":1683696995000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10815-006-9079-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12,20]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,2,2]]}},"alternative-id":["9079"],"URL":"https:\/\/doi.org\/10.1007\/s10815-006-9079-4","relation":{},"ISSN":["1058-0468","1573-7330"],"issn-type":[{"value":"1058-0468","type":"print"},{"value":"1573-7330","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12,20]]}}}