{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:24:56Z","timestamp":1774437896314,"version":"3.50.1"},"reference-count":7,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Assist Reprod Genet"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Introduction<\/jats:title>\n                <jats:p>Whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be detected in semen and transmitted sexually is a vital question that has, thus far, been inconclusive. Prior studies, with limited numbers, have included men in various stages of infection with most in the recovery phase of the illness. The timing of test results and severity of illness has made recruiting study participants a significant challenge. Our pilot study will examine semen from men with a recent diagnosis of COVID-19 as well as those in the convalescent phase to determine if SARS-CoV-2 can be detected and its relationship, if any, with the severity of the disease.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Eighteen men with a median age of 32 (range, 24-57) who tested positive for COVID-19 by rt-PCR analysis were enrolled and provided a semen sample. The study group demonstrated symptoms of COVID-19 ranging from asymptomatic to moderate and none required hospitalization. Samples were subjected to viral RNA extraction and then processed by real-time RT-PCR using the US Centers for Disease Control and Prevention (CDC, USA) panel of 2019-Novel Coronavirus (2019-nCoV) primers and probes to detect the presence of SARS-CoV-2 RNA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Length of time from diagnosis to providing a specimen ranged from 1 to 28 days (median, 6 days). Fifteen participants were symptomatic and three were asymptomatic, including recovering men, at the time of semen collection. No SARS-CoV-2 was detected in any of the semen samples.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Based on these preliminary results and consistent with prior findings, we suggest SARS-CoV-2 is not present in semen during the acute or convalescent phase of COVID-19.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10815-021-02119-y","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T18:42:50Z","timestamp":1614796970000},"page":"785-789","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["A cohort study of men infected with COVID-19 for presence of SARS-CoV-2 virus in their semen"],"prefix":"10.1007","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5326-1341","authenticated-orcid":false,"given":"Corey A.","family":"Burke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AB","family":"Skytte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saghar","family":"Kasiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dixie","family":"Howell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zamip P.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark P.","family":"Trolice","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijo J.","family":"Parekattil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott F.","family":"Michael","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren M.","family":"Paul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,3]]},"reference":[{"issue":"6","key":"2119_CR1","first-page":"P1140","volume":"113","author":"J Segars","year":"2020","unstructured":"Segars J, Katler Q, McQuees D, Toner J, Kawwass J. Prior and novel coronaviruses, coronavirus disease 2019 (COVID-19), and human reproduction: what is known? Fertnstert. 2020;113(6):P1140\u20139.","journal-title":"Fertnstert"},{"key":"2119_CR2","volume-title":"Guidance Document Patient management and clinical recommendations during the Coronavirus (COVID-19) pandemic March 17","author":"ASRM","year":"2020","unstructured":"ASRM Guidance Document Patient management and clinical recommendations during the Coronavirus (COVID-19) pandemic March 17, 2020"},{"key":"2119_CR3","doi-asserted-by":"crossref","unstructured":"ESHRE Assisted reproduction and COVID-19 A statement from ESHRE for phase 1 - guidance on fertility services during pandemic; 2nd April 2020; ESHRE Website www.eshre.eu","DOI":"10.1093\/hropen\/hoaa033"},{"issue":"2","key":"2119_CR4","first-page":"P233","volume":"114","author":"N Holtmann","year":"2020","unstructured":"Holtmann N, Edimiris P, Andree M, Doehmen C, Baston-Buest D, Adams O, et al. Assessment of SARS-CoV-2 in human semen - a cohort study. Fertnstert. 2020;114(2):P233\u20138.","journal-title":"Fertnstert"},{"issue":"6","key":"2119_CR5","first-page":"P1135","volume":"113","author":"F Pan","year":"2020","unstructured":"Pan F, Xiao X, et al. No evidence of severe acute respiratory syndrome\u2013coronavirus 2 in semen of males recovering from coronavirus disease 2019. Fertnstert. 2020;113(6):P1135\u20139.","journal-title":"Fertnstert"},{"key":"2119_CR6","doi-asserted-by":"publisher","unstructured":"Li D, Jin M, Bao P, Zhao W, Zhang S. Clinical characteristics and results of semen test among men with coronavirus disease 2019. JAMA Infect Dis. 2020. https:\/\/doi.org\/10.1001\/jamanetworkopen.2020.8292.","DOI":"10.1001\/jamanetworkopen.2020.8292"},{"issue":"9-10","key":"2119_CR7","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1159\/000510531","volume":"104","author":"B Kyaaaslan","year":"2020","unstructured":"Kyaaaslan B, Korukluoglu G, Hasanoglu I, Kalem A, Eser F, Akinci E, et al. Investigation of SARS-CoV-2 in semen of patients in the acute stage of COVID-19 infection. Urol Int. 2020;104(9-10):678\u201383.","journal-title":"Urol Int"}],"container-title":["Journal of Assisted Reproduction and Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-021-02119-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10815-021-02119-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-021-02119-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T04:03:20Z","timestamp":1619582600000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10815-021-02119-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,3]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["2119"],"URL":"https:\/\/doi.org\/10.1007\/s10815-021-02119-y","relation":{},"ISSN":["1058-0468","1573-7330"],"issn-type":[{"value":"1058-0468","type":"print"},{"value":"1573-7330","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,3]]},"assertion":[{"value":"24 November 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 February 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 March 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This research was approved by Western Institutional Review Board and is in full compliance with good clinical practices as defined under FDA regulations, HHS regulations, and the International Conference on Harmonization (ICH) guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}}]}}