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The trophoblast cell line HTR8\/SVneo was used for silencing NEAT1 or miR-217 inhibitor in the absence or presence of an inhibitor and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Cell counting Kit 8 (CCK-8), flow cytometry, and Transwell were used to detect cell proliferation, apoptosis, migration, and invasion. Luciferase reporter gene assay was utilized to verify the binding between miR-217 and Wnt family member 3 (Wnt3), and between the miR-217 and NEAT1. Proteins related to the Wnt\/\u03b2-catenin signaling pathway were detected using western blotting.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The PE group exhibited a significantly downregulated expression of miR-217 and a significantly upregulated expression of NEAT1. NEAT1 targeted miR-217, and Wnt is a miR-217 target gene. siRNA-NEAT1 inhibited the apoptosis of trophoblast cells, but promoted their invasion, migration, and proliferation. MiR-217 inhibitor could partially reverse the effects of siRNA-NEAT1. The expression of the Wnt\/\u03b2-catenin signaling pathway-related proteins, WNT signaling pathway inhibitor 1 (DKK1), cyclin-D1 and \u03b2-catenin, was significantly increased after siRNA-NEAT1.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>NEAT1 could reduce trophoblast cell invasion and migration by suppressing miR-217\/Wnt signaling pathway, leading to PE.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10815-024-03124-7","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T13:01:46Z","timestamp":1715000506000},"page":"2107-2115","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["LncRNA-NEAT1 blocks the Wnt\/\u03b2-catenin signaling pathway by targeting miR-217 to inhibit trophoblast cell migration and invasion"],"prefix":"10.1007","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9774-1301","authenticated-orcid":false,"given":"Ling-ling","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan-lin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua-le","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mian","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-ying","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"issue":"4","key":"3124_CR1","doi-asserted-by":"publisher","first-page":"e12823","DOI":"10.1111\/aji.12823","volume":"79","author":"C Agostinis","year":"2018","unstructured":"Agostinis C, Rami D, Zacchi P, Bossi F, Stampalija T, Mangogna A, et al. 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This study obtained informed consent from each participant. Approval was granted by the Ethics Committee of Fujian Maternity and Child Health Hospital (2019-115).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no potential conflict of interest with respect to the authorship and publication of this article.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}