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The precise molecular mechanisms underlying these reductions have yet to be fully elucidated. Herein, we examine spatiotemporal distribution of key proteins responsible for DNA double-strand break (DSB) repair in ovaries from early to older ages. Functional studies have shown that the \u03b3H2AX, RAD51, BRCA1, and RPA70 proteins play indispensable roles in HR-based repair pathway, while the KU80 and XRCC4 proteins are essential for successfully operating cNHEJ pathway.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Female Balb\/C mice were divided into five groups as follows: Prepuberty (3\u00a0weeks old; n\u2009=\u20096), puberty (7\u00a0weeks old; n\u2009=\u20097), postpuberty (18\u00a0weeks old; n\u2009=\u20097), early aged (52\u00a0weeks old; n\u2009=\u20097), and late aged (60\u00a0weeks old; n\u2009=\u20097). The expression of DSB repair proteins, cellular senescence (\u03b2-GAL) and apoptosis (cCASP3) markers was evaluated in the ovaries using immunohistochemistry.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Result<\/jats:title>\n                <jats:p>\u03b2-GAL and cCASP3 levels progressively increased from prepuberty to aged groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Notably, \u03b3H2AX levels varied in preantral and antral follicles among the groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). In aged groups, RAD51, BRCA1, KU80, and XRCC4 levels increased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), while RPA70 levels decreased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) compared to the other groups.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The observed alterations were primarily attributed to altered expression in oocytes and granulosa cells of the follicles and other ovarian cells. As a result, the findings indicate that these DSB repair proteins may play a role in the repair processes and even other related cellular events in ovarian cells from early to older ages.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10815-024-03189-4","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T04:01:36Z","timestamp":1721275296000},"page":"2419-2439","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The DNA double-strand break repair proteins \u03b3H2AX, RAD51, BRCA1, RPA70, KU80, and XRCC4 exhibit follicle-specific expression differences in the postnatal mouse ovaries from early to older ages"],"prefix":"10.1007","volume":"41","author":[{"given":"Gunel","family":"Talibova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yesim","family":"Bilmez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betul","family":"Tire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8089-7078","authenticated-orcid":false,"given":"Saffet","family":"Ozturk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,18]]},"reference":[{"issue":"1","key":"3189_CR1","doi-asserted-by":"publisher","first-page":"16","DOI":"10.5653\/cerm.2021.04917","volume":"49","author":"CJ Park","year":"2022","unstructured":"Park CJ, et al. 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