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Recently, a method to quantify spermatozoa centriolar defects was developed named Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC). However, whether spermatozoa centriolar defects identified by FRAC can result in abnormal zygote functions was not tested.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Here, we quantified spermatozoa centriolar defects using FRAC, and zygote centriole function was assessed by imaging Nucleolus Precursor Body (NPB) polarization which was based on the pattern of NPB polarization. Data was analyzed at couple and embryo levels. Subjects were divided into two groups: seven couples and 62 embryos with normal spermatozoa centrioles versus eight couples and 78 embryos with abnormal spermatozoa centrioles (140 embryos from 15 couples in total).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Patterned NPB polarization was statistically significant in both couple- and embryo-level analyses (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001 and <jats:italic>p<\/jats:italic>\u2009=\u20090.0024). These results suggest that the abnormal spermatozoa centrioles identified by FRAC may correlate with abnormal zygote centrosome function via NPB polarization scoring.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This study provides a foundation for more extensive studies to test for FRAC\u2019s utility in assessing spermatozoa centriole quality.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s10815-025-03411-x","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T15:54:09Z","timestamp":1738943649000},"page":"1121-1132","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Spermatozoa centriole quality determined by FRAC may correlate with zygote nucleoli polarization\u2014a pilot study"],"prefix":"10.1007","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0977-4961","authenticated-orcid":false,"given":"Derek F.","family":"Kluczynski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0078-3602","authenticated-orcid":false,"given":"Ankit","family":"Jaiswal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1335-0580","authenticated-orcid":false,"given":"Min","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3408-3206","authenticated-orcid":false,"given":"Nagalakshmi","family":"Nadiminty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7337-5749","authenticated-orcid":false,"given":"Barbara","family":"Saltzman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5524-3649","authenticated-orcid":false,"given":"Samantha","family":"Schon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0918-526X","authenticated-orcid":false,"given":"Tomer","family":"Avidor-Reiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"issue":"3","key":"3411_CR1","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.fertnstert.2019.11.025","volume":"113","author":"Practice Committee of the American Society for Reproductive Medicine","year":"2020","unstructured":"Practice Committee of the American Society for Reproductive Medicine. 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Am J Pathol. 1999;155(6):1941\u201351.","journal-title":"Am J Pathol"}],"container-title":["Journal of Assisted Reproduction and Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-025-03411-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10815-025-03411-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-025-03411-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T09:36:25Z","timestamp":1746524185000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10815-025-03411-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,7]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["3411"],"URL":"https:\/\/doi.org\/10.1007\/s10815-025-03411-x","relation":{},"ISSN":["1058-0468","1573-7330"],"issn-type":[{"value":"1058-0468","type":"print"},{"value":"1573-7330","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,7]]},"assertion":[{"value":"9 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Collaborative Program for Translating Basic Research to Clinical Trial in Idiopathic Infertility (CPTBR) consortium was formed in November 2018 to study male infertility using multiparametric sperm analysis. The semen samples were collected from the Reproductive Subject Registry and Sample Repository (RSRSR) at the University of Michigan (UM IRB#-HUM00125627). This study was conducted in accordance with the Declaration of Helsinki, and approved by the University of Toledo Institutional Review Board (IRB) (UT IRB#300364; initial approval 10\/11\/2019; PI: Tomer Avidor-Reiss). The remaining members of the CPTBR consortium agreed to rely on the approval of the study by the University of Toledo by employing a SMART IRB Reliance system (; SMART IRB ID: 2683; initial approval 4\/11\/2020). The University of Toledo IRB reviewed the SMART IRB, and other consortium members relied on the University of Toledo IRB for compliance with human subject research protections.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Informed consent was obtained from all subjects involved in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"The University of Toledo has filed provisional patent application number 63\/117,056, with TAR named on this patent based on the original article describing the FRAC method.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Patents"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}