{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T18:18:44Z","timestamp":1770229124691,"version":"3.49.0"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Prisma Health Transformative Seed Grant"},{"DOI":"10.13039\/100008899","name":"University of South Carolina","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008899","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Assist Reprod Genet"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Purpose<\/jats:title>\n            <jats:p>This retrospective study measured global gene abundance using RNASeq of blastocoel fluid-conditioned media from euploid ICSI-generated embryos to identify genes and signaling pathways associated with maternal age.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Blastocoel fluid-conditioned media was obtained following trophectoderm biopsy of ICSI-generated day-5 blastocysts. Media for RNASeq were from 24 euploid blastocysts (9 from patients aged 35 or older). Transcriptome analysis identified differentially expressed genes when comparing media from patients of advanced maternal age to those younger than 35. Further gene abundance analysis on genes and pathways identified from the RNASeq analysis was conducted with another group of media samples using RT-qPCR.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Twenty-five protein encoding genes identified in the RNASeq study were differentially expressed when comparing blastocoel fluid-conditioned media associated with patients of advanced maternal age to media associated with patients under the age of 35. Genes encoding the proteins SHARPIN and BCL2L12 showed a statistically significant increase (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in abundance in patients of advanced maternal age. Abundance analysis using RT-qPCR in additional media samples revealed elevated <jats:italic>SHARPIN<\/jats:italic> abundance in media associated with successful implantation in patients under 35 alongside a decrease in <jats:italic>CASP8<\/jats:italic> abundance. This abundance pattern was the opposite in media associated with successful implantation in patients of advanced maternal age.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This study uncovered differential apoptotic gene abundance associated with maternal age by assessing blastocoel fluid-conditioned media from euploid blastocysts. These unique abundance patterns may provide insight into the regulation of apoptosis in embryos from women of advanced maternal age, and how this signaling pathway may impact implantation outcomes.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s10815-025-03485-7","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T08:17:33Z","timestamp":1745309853000},"page":"1651-1661","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Maternal age is associated with apoptotic gene abundance patterns in blastocoel fluid-conditioned media from euploid embryos: a pilot study"],"prefix":"10.1007","volume":"42","author":[{"given":"Dieu Thao","family":"Nguyen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Archer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel","family":"Earle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleanor","family":"Petyak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carson","family":"Collins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla","family":"Vaillant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayes","family":"Lanford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E.","family":"Roudebush","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. Arthur","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rich","family":"Kordus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa","family":"Clay-Gilmour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0442-0833","authenticated-orcid":false,"given":"Renee J.","family":"Chosed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"3485_CR1","unstructured":"American College of Obstetricians and Gynecologists Committee on Gynecologic Practice and Practice Committee. Female age-related fertility decline. Committee Opinion No. 589. Fertil Steril. 2014;101:633\u20134."},{"key":"3485_CR2","doi-asserted-by":"publisher","first-page":"1180689","DOI":"10.3389\/fmolb.2023.1180689","volume":"10","author":"A Martin","year":"2023","unstructured":"Martin A, Mercader A, Dominguez F, Qui\u00f1onero A, Perez M, Gonzalez-Martin R, et al. Mosaic results after preimplantation genetic testing for aneuploidy may be accompanied by changes in global gene expression. Front Mol Biosci. 2023;10:1180689.","journal-title":"Front Mol Biosci"},{"key":"3485_CR3","doi-asserted-by":"publisher","first-page":"94","DOI":"10.3389\/fendo.2019.00094","volume":"10","author":"FM Ubaldi","year":"2019","unstructured":"Ubaldi FM, Cimadomo D, Vaiarelli A, Fabozzi G, Venturella R, Maggiulli R, et al. Advanced maternal age in IVF: still a challenge? The present and the future of its treatment. Front Endocrinol. 2019;10:94.","journal-title":"Front Endocrinol"},{"key":"3485_CR4","unstructured":"SART final national summary report for 2022. [Internet]. Available from: https:\/\/sartcorsonline.com\/CSR\/PublicSnapshotReport?ClinicPKID=0&reportingYear=2022."},{"key":"3485_CR5","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1186\/s13048-023-01151-z","volume":"16","author":"X Wang","year":"2023","unstructured":"Wang X, Wang L, Xiang W. Mechanisms of ovarian aging in women: a review. J Ovarian Res. 2023;16:67.","journal-title":"J Ovarian Res"},{"key":"3485_CR6","doi-asserted-by":"publisher","first-page":"602","DOI":"10.3390\/genes11060602","volume":"11","author":"M Viotti","year":"2020","unstructured":"Viotti M. Preimplantation genetic testing for chromosomal abnormalities: aneuploidy, mosaicism, and structural rearrangements. Genes. 2020;11:602.","journal-title":"Genes"},{"key":"3485_CR7","doi-asserted-by":"crossref","unstructured":"Practice Committees of the American Society for Reproductive Medicine and the Genetic Counseling Professional Group. Clinical management of mosaic results from preimplantation genetic testing for aneuploidy of blastocysts: a committee opinion. Fertil Steril. 2023;120:973\u201382.","DOI":"10.1016\/j.fertnstert.2023.08.969"},{"key":"3485_CR8","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1007\/s10815-020-01720-x","volume":"37","author":"NM Sachdev","year":"2020","unstructured":"Sachdev NM, McCulloh DH, Kramer Y, Keefe D, Grifo JA. The reproducibility of trophectoderm biopsies in euploid, aneuploid, and mosaic embryos using independently verified next-generation sequencing (NGS): a pilot study. J Assist Reprod Genet. 2020;37:559\u201371.","journal-title":"J Assist Reprod Genet"},{"key":"3485_CR9","doi-asserted-by":"publisher","first-page":"2339","DOI":"10.1093\/humrep\/deab117","volume":"36","author":"SJ Bhatt","year":"2021","unstructured":"Bhatt SJ, Marchetto NM, Roy J, Morelli SS, McGovern PG. Pregnancy outcomes following in vitro fertilization frozen embryo transfer (IVF-FET) with or without preimplantation genetic testing for aneuploidy (PGT-A) in women with recurrent pregnancy loss (RPL): a SART-CORS study. Hum Reprod. 2021;36:2339\u201344.","journal-title":"Hum Reprod"},{"key":"3485_CR10","doi-asserted-by":"publisher","first-page":"2047","DOI":"10.1056\/NEJMoa2103613","volume":"385","author":"J Yan","year":"2021","unstructured":"Yan J, Qin Y, Zhao H, Sun Y, Gong F, Li R, et al. Live birth with or without preimplantation genetic testing for aneuploidy. N Engl J Med. 2021;385:2047\u201358.","journal-title":"N Engl J Med"},{"key":"3485_CR11","doi-asserted-by":"publisher","first-page":"104322","DOI":"10.1016\/j.rbmo.2024.104322","volume":"49","author":"G Campos","year":"2024","unstructured":"Campos G, Nel-Themaat L. Blastocoel fluid as an alternative source of DNA for minimally invasive PGT and biomarker of embryo competence. Reprod Biomed Online. 2024;49:104322.","journal-title":"Reprod Biomed Online"},{"key":"3485_CR12","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s10815-023-03015-3","volume":"41","author":"JFC Chow","year":"2024","unstructured":"Chow JFC, Lam KKW, Cheng HHY, Lai SF, Yeung WSB, Ng EHY. Optimizing non-invasive preimplantation genetic testing: investigating culture conditions, sample collection, and IVF treatment for improved non-invasive PGT-A results. J Assist Reprod Genet. 2024;41:465\u201372.","journal-title":"J Assist Reprod Genet"},{"key":"3485_CR13","doi-asserted-by":"publisher","first-page":"1268","DOI":"10.1093\/humrep\/dead088","volume":"38","author":"L Gianaroli","year":"2023","unstructured":"Gianaroli L, Perruzza D, Albanese C, Azzena S, Tabanelli C, Ferraretti AP, et al. Failure to detect DNA in blastocoel fluid is associated with a higher live birth rate in both PGT-A and conventional IVF\/ICSI cycles. Hum Reprod. 2023;38:1268\u201376.","journal-title":"Hum Reprod"},{"key":"3485_CR14","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1186\/s12884-023-05666-7","volume":"23","author":"R Liang","year":"2023","unstructured":"Liang R, Duan SN, Fu M, Chen YN, Wang P, Fan Y, et al. Prediction model for day 3 embryo implantation potential based on metabolites in spent embryo culture medium. BMC Pregnancy Childbirth. 2023;23:425.","journal-title":"BMC Pregnancy Childbirth"},{"key":"3485_CR15","doi-asserted-by":"publisher","first-page":"1685","DOI":"10.1007\/s10815-020-01796-5","volume":"37","author":"D Makri","year":"2020","unstructured":"Makri D, Efstathiou P, Michailidou E, Maalouf WE. Apoptosis triggers the release of microRNA miR-294 in spent culture media of blastocysts. J Assist Reprod Genet. 2020;37:1685\u201394.","journal-title":"J Assist Reprod Genet"},{"key":"3485_CR16","doi-asserted-by":"publisher","first-page":"1515","DOI":"10.1007\/s10815-022-02510-3","volume":"39","author":"A Lal","year":"2022","unstructured":"Lal A, Kranyak A, Blalock J, Athavale D, Barr\u00e9 A, Doran A, et al. Apoptotic qPCR gene expression array analysis demonstrates proof-of-concept for rapid blastocoel fluid-conditioned media molecular prediction. J Assist Reprod Genet. 2022;39:1515\u201322.","journal-title":"J Assist Reprod Genet"},{"key":"3485_CR17","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1007\/s10815-023-02813-z","volume":"40","author":"N Handayani","year":"2023","unstructured":"Handayani N, Aubry D, Boediono A, Wiweko B, Sirait B, Sini I, et al. The origin and possible mechanism of embryonic cell-free DNA release in spent embryo culture media: a review. J Assist Reprod Genet. 2023;40:1231\u201342.","journal-title":"J Assist Reprod Genet"},{"key":"3485_CR18","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1530\/ror.0.0040125","volume":"4","author":"K Hardy","year":"1999","unstructured":"Hardy K. Apoptosis in the human embryo. Rev Reprod. 1999;4:125\u201334.","journal-title":"Rev Reprod"},{"key":"3485_CR19","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1038\/s41556-021-00660-7","volume":"23","author":"M Yang","year":"2021","unstructured":"Yang M, Rito T, Metzger J, Naftaly J, Soman R, Hu J, et al. Depletion of aneuploid cells in human embryos and gastruloids. Nat Cell Biol. 2021;23:314\u201321.","journal-title":"Nat Cell Biol"},{"key":"3485_CR20","doi-asserted-by":"publisher","first-page":"18","DOI":"10.3390\/genes15010018","volume":"15","author":"G Campos","year":"2023","unstructured":"Campos G, Sciorio R, Fleming S. Healthy live births after the transfer of mosaic embryos: self-correction or PGT-A overestimation? Genes. 2023;15:18.","journal-title":"Genes"},{"key":"3485_CR21","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1186\/s13048-024-01427-y","volume":"17","author":"S Bao","year":"2024","unstructured":"Bao S, Yin T, Liu S. Ovarian aging: energy metabolism of oocytes. J Ovarian Res. 2024;17:118.","journal-title":"J Ovarian Res"},{"key":"3485_CR22","doi-asserted-by":"publisher","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","volume":"32","author":"P Ewels","year":"2016","unstructured":"Ewels P, Magnusson M, Lundin S, K\u00e4ller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016;32:3047\u20138.","journal-title":"Bioinformatics"},{"key":"3485_CR23","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","volume":"30","author":"Y Liao","year":"2014","unstructured":"Liao Y, Smyth GK, Shi W. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics. 2014;30:923\u201330.","journal-title":"Bioinformatics"},{"key":"3485_CR24","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","volume":"29","author":"A Dobin","year":"2013","unstructured":"Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013;29:15\u201321.","journal-title":"Bioinformatics"},{"key":"3485_CR25","doi-asserted-by":"publisher","first-page":"10","DOI":"10.14806\/ej.17.1.200","volume":"17","author":"M Martin","year":"2011","unstructured":"Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 2011;17:10\u20132.","journal-title":"EMBnet J"},{"key":"3485_CR26","unstructured":"Andrews, Simon. FastQC: a quality control tool for high throughput sequence data [Internet]. Available from: \nhttp:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"3485_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2174\/1875036201307010001","volume":"7","author":"E Aronesty","year":"2013","unstructured":"Aronesty E. Comparison of sequencing utility programs. TOBIOIJ. 2013;7:1\u20138.","journal-title":"TOBIOIJ"},{"key":"3485_CR28","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","volume":"30","author":"AM Bolger","year":"2014","unstructured":"Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30:2114\u201320.","journal-title":"Bioinformatics"},{"key":"3485_CR29","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1093\/bioinformatics\/btr026","volume":"27","author":"R Schmieder","year":"2011","unstructured":"Schmieder R, Edwards R. Quality control and preprocessing of metagenomic datasets. Bioinformatics. 2011;27:863\u20134.","journal-title":"Bioinformatics"},{"key":"3485_CR30","doi-asserted-by":"publisher","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","volume":"10","author":"B Langmead","year":"2009","unstructured":"Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10:R25.","journal-title":"Genome Biol"},{"key":"3485_CR31","doi-asserted-by":"publisher","first-page":"D64","DOI":"10.1093\/nar\/gks1048","volume":"41","author":"LR Meyer","year":"2012","unstructured":"Meyer LR, Zweig AS, Hinrichs AS, Karolchik D, Kuhn RM, Wong M, et al. The UCSC Genome Browser database: extensions and updates 2013. Nucleic Acids Res. 2012;41:D64\u20139.","journal-title":"Nucleic Acids Res"},{"key":"3485_CR32","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, et al. The sequence alignment\/map format and SAMtools. Bioinformatics. 2009;25:2078\u20139.","journal-title":"Bioinformatics"},{"key":"3485_CR33","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","volume":"26","author":"AR Quinlan","year":"2010","unstructured":"Quinlan AR, Hall IM. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 2010;26:841\u20132.","journal-title":"Bioinformatics"},{"key":"3485_CR34","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1261\/rna.2183803","volume":"9","author":"V Ambros","year":"2003","unstructured":"Ambros V, Bartel B, Bartel DP, Burge CB, Carrington JC, Chen X, et al. A uniform system for microRNA annotation. RNA. 2003;9:277\u20139.","journal-title":"RNA"},{"key":"3485_CR35","doi-asserted-by":"publisher","first-page":"D756","DOI":"10.1093\/nar\/gkt1114","volume":"42","author":"KD Pruitt","year":"2014","unstructured":"Pruitt KD, Brown GR, Hiatt SM, Thibaud-Nissen F, Astashyn A, Ermolaeva O, et al. RefSeq: an update on mammalian reference sequences. Nucl Acids Res. 2014;42:D756\u201363.","journal-title":"Nucl Acids Res"},{"key":"3485_CR36","doi-asserted-by":"publisher","first-page":"1915","DOI":"10.1101\/gad.17446611","volume":"25","author":"MN Cabili","year":"2011","unstructured":"Cabili MN, Trapnell C, Goff L, Koziol M, Tazon-Vega B, Regev A, et al. Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses. Genes Dev. 2011;25:1915\u201327.","journal-title":"Genes Dev"},{"key":"3485_CR37","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014;15:550.","journal-title":"Genome Biol"},{"key":"3485_CR38","doi-asserted-by":"publisher","first-page":"15545","DOI":"10.1073\/pnas.0506580102","volume":"102","author":"A Subramanian","year":"2005","unstructured":"Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA. 2005;102:15545\u201350.","journal-title":"Proc Natl Acad Sci USA"},{"key":"3485_CR39","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1038\/ng1180","volume":"34","author":"VK Mootha","year":"2003","unstructured":"Mootha VK, Lindgren CM, Eriksson K-F, Subramanian A, Sihag S, Lehar J, et al. PGC-1\u03b1-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat Genet. 2003;34:267\u201373.","journal-title":"Nat Genet"},{"key":"3485_CR40","doi-asserted-by":"publisher","first-page":"858505","DOI":"10.3389\/fimmu.2022.858505","volume":"13","author":"B Yu","year":"2022","unstructured":"Yu B, Wang F, Wang Y. Advances in the structural and physiological functions of SHARPIN. Front Immunol. 2022;13:858505.","journal-title":"Front Immunol"},{"key":"3485_CR41","doi-asserted-by":"publisher","first-page":"e03422","DOI":"10.7554\/eLife.03422","volume":"3","author":"S Kumari","year":"2014","unstructured":"Kumari S, Redouane Y, Lopez-Mosqueda J, Shiraishi R, Romanowska M, Lutzmayer S, et al. Sharpin prevents skin inflammation by inhibiting TNFR1-induced keratinocyte apoptosis. eLife. 2014;3:e03422.","journal-title":"eLife"},{"key":"3485_CR42","doi-asserted-by":"publisher","first-page":"6246","DOI":"10.1038\/s41467-022-34042-w","volume":"13","author":"Z Wang","year":"2022","unstructured":"Wang Z, Wang S, Qin J, Zhang X, Lu G, Liu H, et al. Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12. Nat Commun. 2022;13:6246.","journal-title":"Nat Commun"},{"key":"3485_CR43","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1007\/s10815-019-01441-w","volume":"36","author":"RT Michita","year":"2019","unstructured":"Michita RT, Zambra FMB, Fraga LR, Sanseverino MT, Schuler-Faccini L, Chies JAB, et al. The role of FAS, FAS-L, BAX, and BCL-2 gene polymorphisms in determining susceptibility to unexplained recurrent pregnancy loss. J Assist Reprod Genet. 2019;36:995\u20131002.","journal-title":"J Assist Reprod Genet"},{"key":"3485_CR44","doi-asserted-by":"publisher","first-page":"11165","DOI":"10.1038\/ncomms11165","volume":"7","author":"H Bolton","year":"2016","unstructured":"Bolton H, Graham SJL, Van Der Aa N, Kumar P, Theunis K, Fernandez Gallardo E, et al. Mouse model of chromosome mosaicism reveals lineage-specific depletion of aneuploid cells and normal developmental potential. Nat Commun. 2016;7:11165.","journal-title":"Nat Commun"},{"key":"3485_CR45","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1007\/s10815-022-02698-4","volume":"40","author":"N Adel","year":"2023","unstructured":"Adel N, Abdulghaffar S, Elmahdy M, Nabil M, Ghareeb D, Maghraby H. Autophagy-related gene and protein expressions during blastocyst development. J Assist Reprod Genet. 2023;40:323\u201331.","journal-title":"J Assist Reprod Genet"},{"key":"3485_CR46","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1016\/j.fertnstert.2008.07.1761","volume":"92","author":"S Barbash-Hazan","year":"2009","unstructured":"Barbash-Hazan S, Frumkin T, Malcov M, Yaron Y, Cohen T, Azem F, et al. Preimplantation aneuploid embryos undergo self-correction in correlation with their developmental potential. Fertil Steril. 2009;92:890\u20136.","journal-title":"Fertil Steril"},{"key":"3485_CR47","doi-asserted-by":"publisher","first-page":"2958","DOI":"10.1038\/s41467-020-16796-3","volume":"11","author":"S Singla","year":"2020","unstructured":"Singla S, Iwamoto-Stohl LK, Zhu M, Zernicka-Goetz M. Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism. Nat Commun. 2020;11:2958.","journal-title":"Nat Commun"},{"key":"3485_CR48","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1093\/humrep\/dez012","volume":"34","author":"M Popovic","year":"2019","unstructured":"Popovic M, Dhaenens L, Taelman J, Dheedene A, Bialecka M, De Sutter P, et al. Extended in vitro culture of human embryos demonstrates the complex nature of diagnosing chromosomal mosaicism from a single trophectoderm biopsy. Hum Reprod. 2019;34:758\u201369.","journal-title":"Hum Reprod"},{"key":"3485_CR49","doi-asserted-by":"publisher","first-page":"658048","DOI":"10.3389\/fimmu.2021.658048","volume":"12","author":"J Wang","year":"2021","unstructured":"Wang J, Li T, Zan H, Rivera CE, Yan H, Xu Z. LUBAC suppresses IL-21-induced apoptosis in CD40-activated murine B cells and promotes germinal center B cell survival and the T-dependent antibody response. Front Immunol. 2021;12:658048.","journal-title":"Front Immunol"}],"container-title":["Journal of Assisted Reproduction and Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-025-03485-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10815-025-03485-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10815-025-03485-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T12:01:53Z","timestamp":1749902513000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10815-025-03485-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,22]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["3485"],"URL":"https:\/\/doi.org\/10.1007\/s10815-025-03485-7","relation":{},"ISSN":["1058-0468","1573-7330"],"issn-type":[{"value":"1058-0468","type":"print"},{"value":"1573-7330","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,22]]},"assertion":[{"value":"11 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Prisma Health IRB approved this study and required patient consent for the blastocoel fluid-conditioned media samples obtained at Fertility Center of the Carolinas at Prisma Health that was used for this study. This research study was conducted retrospectively from data obtained for clinical purposes.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Informed consent was obtained from all individual participants included in the study at Fertility Center of the Carolinas at Prisma Health.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}