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There is an increased risk of chromosomal abnormalities for patients with advanced maternal age, recurrent implantation failure and recurrent pregnancy loss. To address this, preimplantation genetic testing for aneuploidy has evolved through several methods since the 1990s, when it first began through fluorescence in situ hybridisation. The limitations of these early methods were overcome with the progression in technology that enabled the screening of all 23 chromosome pairs. These methods included microarray methods and next-generation sequencing platforms. Currently, these are used for PGT-A; however, for IVF clinics to carry out PGT-A utilising these methods, samples are sent to external laboratories capable of carrying out these methods. More recently, nanopore sequencing has emerged, which may overcome these limitations and be carried out within IVF clinics to facilitate faster patient results. This review focuses on the evolution of PGT-A methods through next-generation sequencing and the current status of these methods.\n<\/jats:p>","DOI":"10.1007\/s10815-025-03576-5","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T11:27:18Z","timestamp":1752233238000},"page":"2483-2496","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Changes in sequencing technology used for preimplantation genetic testing for aneuploidy"],"prefix":"10.1007","volume":"42","author":[{"given":"Vivianne Elizabeth","family":"Oltramare","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3118-3084","authenticated-orcid":false,"given":"Catherine","family":"McDermott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4753-7430","authenticated-orcid":false,"given":"Paul Joseph","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,11]]},"reference":[{"key":"3576_CR1","doi-asserted-by":"publisher","first-page":"667697","DOI":"10.3389\/fgene.2021.667697","volume":"12","author":"C Gu","year":"2021","unstructured":"Gu C, Li K, Li R, Li L, Li X, Dai X, He Y. 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We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us. We confirm that we have given due consideration to the protection of intellectual property associated with this work and that there are no impediments to publication, including the timing of publication, with respect to intellectual property. In so doing, we confirm that we have followed the regulations of our institutions concerning intellectual property. We understand that the Corresponding Author is the sole contact for the Editorial process (including Editorial Manager and direct communications with the office). They are responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs. We confirm that we have provided a current, correct email address which is accessible by the Corresponding Author.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}