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To prevent this complication and seal the dura watertight, we developed Liqoseal, a dural sealant patch comprising a watertight polyesterurethane layer and an adhesive layer consisting of poly(DL-lactide-co-\u03b5-caprolactone) copolymer and multiarmed N-hydroxylsuccinimide functionalized polyethylene glycol. We compared acute burst pressure and resistance to physiological conditions for 72\u2009h of Liqoseal, Adherus, Duraseal, Tachosil, and Tisseel using computer-assisted models and fresh porcine dura. The mean acute burst pressure of Liqoseal in the cranial model (145\u2009\u00b1\u200939\u2009mmHg) was higher than that of Adherus (87\u2009\u00b1\u200947\u2009mmHg), Duraseal (51\u2009\u00b1\u200942\u2009mmHg) and Tachosil (71\u2009\u00b1\u200916\u2009mmHg). Under physiological conditions, cranial model resistance test results showed that 2 of 3 Liqoseal sealants maintained dural attachment during 72 hours as opposed to 3 of 3 for Adherus and Duraseal and 0 of 3 for Tachosil. The mean burst pressure of Liqoseal in the spinal model (233\u2009\u00b1\u200981\u2009mmHg) was higher than that of Tachosil (123\u2009\u00b1\u200963\u2009mmHg) and Tisseel (23\u2009\u00b1\u200916\u2009mmHg). Under physiological conditions, spinal model resistance test results showed that 2 of 3 Liqoseal sealants maintained dural attachment for 72 hours as opposed to 3 of 3 for Adherus and 0 of 3 for Duraseal and Tachosil. This novel study showed that Liqoseal is capable of achieving a strong watertight seal over a dural defect in ex vivo models.<\/jats:p>","DOI":"10.1007\/s10856-021-06552-4","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T10:02:58Z","timestamp":1627034578000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Ex vivo evaluation of a multilayered sealant patch for watertight dural closure: cranial and spinal models"],"prefix":"10.1007","volume":"32","author":[{"given":"A.","family":"Kinaci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"van Thoor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Redegeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Tooren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6299-3859","authenticated-orcid":false,"given":"T. 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