{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T19:12:40Z","timestamp":1788635560592,"version":"build-2803163510"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:00:00Z","timestamp":1788566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:00:00Z","timestamp":1788566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100018847","name":"Sel\u00e7uk University Research Foundation","doi-asserted-by":"publisher","award":["24202046"],"award-info":[{"award-number":["24202046"]}],"id":[{"id":"10.13039\/501100018847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Bioenerg Biomembr"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1007\/s10863-026-10134-w","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T06:29:31Z","timestamp":1788589771000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neuroprotective effect of 3',4'-dihydroxyflavonol on blood\u2013brain barrier \u0131ntegrity and matrix remodeling in rats with transient bilateral carotid occlusion-\u0131nduced \u0131schemia\u2013reperfusion"],"prefix":"10.1007","volume":"58","author":[{"given":"Delal Ezgi","family":"Akinci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tugce","family":"Aladag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saltuk Bugra","family":"Baltaci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebru K\u00fcbra","family":"Uzdil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6155-6780","authenticated-orcid":false,"given":"Rasim","family":"Mogulkoc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulkerim Kasim","family":"Baltaci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,5]]},"reference":[{"key":"10134_CR1","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.neuint.2018.05.009","volume":"118","author":"A Aggarwal","year":"2018","unstructured":"Aggarwal A, Singh I, Sandhir R (2018) Protective effect of S-nitrosoglutathione administration against hyperglycemia induced disruption of blood brain barrier is mediated by modulation of tight junction proteins and cell adhesion molecules. Neurochem Int 118:205\u2013216","journal-title":"Neurochem Int"},{"key":"10134_CR2","doi-asserted-by":"publisher","first-page":"176670","DOI":"10.1016\/j.ejphar.2024.176670","volume":"976","author":"T Aladag","year":"2024","unstructured":"Aladag T et al (2024) Improvement of neuronal and cognitive functions following treatment with 3\u2032, 4\u2032 dihydroxyflavonol in experimental focal cerebral ischemia-reperfusion injury in rats. Eur J Pharmacol 976:176670","journal-title":"Eur J Pharmacol"},{"issue":"2","key":"10134_CR3","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/0006-8993(93)91379-7","volume":"600","author":"M Asanuma","year":"1993","unstructured":"Asanuma M et al (1993) Ischemia-induced changes in \u03b1-tubulin and \u03b2-actin mRNA in the gerbil brain and effects of bifemelane hydrochloride. Brain Res 600(2):243\u2013248","journal-title":"Brain Res"},{"key":"10134_CR4","doi-asserted-by":"publisher","first-page":"594672","DOI":"10.3389\/fneur.2020.594672","volume":"11","author":"S Bernardo-Castro","year":"2020","unstructured":"Bernardo-Castro S et al (2020) Pathophysiology of blood\u2013brain barrier permeability throughout the different stages of ischemic stroke and its implication on hemorrhagic transformation and recovery. Front Neurol 11:594672","journal-title":"Front Neurol"},{"issue":"2","key":"10134_CR5","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1111\/j.1523-1755.2004.761_2.x","volume":"66","author":"JV Bonventre","year":"2004","unstructured":"Bonventre JV, Zuk A (2004) Ischemic acute renal failure: an inflammatory disease? Kidney Int 66(2):480\u2013485","journal-title":"Kidney Int"},{"key":"10134_CR6","doi-asserted-by":"publisher","DOI":"10.2174\/0113816128360698250103082923","author":"O \u00c7etin","year":"2025","unstructured":"\u00c7etin O et al (2025) 3\u2019, 4\u2019-Dihydroxy flavonol (DiOHF) exerting a positive effect on neurogenesis and retinal damage in experimental brain ischemia-reperfusion of rats. Curr Pharm des. https:\/\/doi.org\/10.2174\/0113816128360698250103082923","journal-title":"Curr Pharm des"},{"issue":"2","key":"10134_CR7","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.brainres.2005.01.027","volume":"1038","author":"S Dohgu","year":"2005","unstructured":"Dohgu S et al (2005) Brain pericytes contribute to the induction and up-regulation of blood-brain barrier functions through transforming growth factor-beta production. Brain Res 1038(2):208\u2013215","journal-title":"Brain Res"},{"issue":"6","key":"10134_CR8","doi-asserted-by":"publisher","first-page":"1366","DOI":"10.3390\/biomedicines12061366","volume":"12","author":"X Fan","year":"2024","unstructured":"Fan X et al (2024) Luteolin-7-O-\u03b2-d-glucuronide attenuated cerebral ischemia\/reperfusion injury: involvement of the blood\u2013brain barrier. Biomedicines 12(6):1366","journal-title":"Biomedicines"},{"issue":"7","key":"10134_CR9","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1111\/bph.16239","volume":"181","author":"L Feng","year":"2024","unstructured":"Feng L et al (2024) Trilobatin attenuates cerebral ischaemia\/reperfusion-induced blood\u2013brain barrier dysfunction by targeting matrix metalloproteinase 9: the legend of a food additive. Br J Pharmacol 181(7):1005\u20131027","journal-title":"Br J Pharmacol"},{"issue":"8","key":"10134_CR10","doi-asserted-by":"publisher","first-page":"1622","DOI":"10.3174\/ajnr.A0628","volume":"28","author":"R Gonzalez","year":"2007","unstructured":"Gonzalez R et al (2007) Acute ischemic stroke: imaging and intervention. Am J Neuroradiol 28(8):1622","journal-title":"Am J Neuroradiol"},{"key":"10134_CR11","doi-asserted-by":"publisher","first-page":"111742","DOI":"10.1016\/j.intimp.2024.111742","volume":"130","author":"X Gu","year":"2024","unstructured":"Gu X et al (2024) Fisetin alleviates cerebral ischemia\/reperfusion injury by regulating Sirt1\/Foxc1\/Ubqln1 pathway-mediated proteostasis. Int Immunopharmacol 130:111742","journal-title":"Int Immunopharmacol"},{"issue":"2","key":"10134_CR12","doi-asserted-by":"publisher","first-page":"1064","DOI":"10.1080\/07391102.2023.2205940","volume":"42","author":"A Janyou","year":"2024","unstructured":"Janyou A et al (2024) Effects of isosakuranetin on cerebral infarction and blood brain barrier damage from cerebral ischemia\/reperfusion injury in a rat model. J Biomol Struct Dyn 42(2):1064\u20131071","journal-title":"J Biomol Struct Dyn"},{"issue":"1","key":"10134_CR13","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/0169-328X(94)00286-N","volume":"30","author":"K Kumar","year":"1995","unstructured":"Kumar K, Wu X-F (1995) Expression of \u03b2-actin and a-tubulin mRNA in gerbil brain following transient ischemia and reperfusion up to 1 month. Mol Brain Res 30(1):149\u2013157","journal-title":"Mol Brain Res"},{"issue":"6","key":"10134_CR14","doi-asserted-by":"publisher","first-page":"e20813","DOI":"10.1371\/journal.pone.0020813","volume":"6","author":"C-H Leo","year":"2011","unstructured":"Leo C-H, Hart JL, Woodman OL (2011) 3\u2032, 4\u2032-Dihydroxyflavonol reduces superoxide and improves nitric oxide function in diabetic rat mesenteric arteries. PLoS ONE 6(6):e20813","journal-title":"PLoS ONE"},{"issue":"1","key":"10134_CR17","doi-asserted-by":"publisher","first-page":"18451","DOI":"10.1038\/s41598-021-97908-x","volume":"11","author":"G Liu","year":"2021","unstructured":"Liu G et al (2021) Protective mechanism of Erigeron breviscapus injection on blood\u2013brain barrier injury induced by cerebral ischemia in rats. Sci Rep 11(1):18451","journal-title":"Sci Rep"},{"issue":"12","key":"10134_CR16","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1038\/s41401-020-0409-3","volume":"41","author":"M-B Liu","year":"2020","unstructured":"Liu M-B et al (2020) Icariside II attenuates cerebral ischemia\/reperfusion-induced blood\u2013brain barrier dysfunction in rats via regulating the balance of MMP9\/TIMP1. Acta Pharmacol Sin 41(12):1547\u20131556","journal-title":"Acta Pharmacol Sin"},{"key":"10134_CR18","doi-asserted-by":"publisher","first-page":"117690","DOI":"10.1016\/j.jep.2023.117690","volume":"323","author":"Z Liu","year":"2024","unstructured":"Liu Z, Han X, You Y, Xin G, Li L, Gao J et al (2024) Shuangshen ningxin formula attenuates cardiac microvascular ischemia\/reperfusion injury through improving mitochondrial function. J Ethnopharmacol 323:117690","journal-title":"J Ethnopharmacol"},{"issue":"4","key":"10134_CR19","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method. Methods 25(4):402\u2013408","journal-title":"Methods"},{"key":"10134_CR15","doi-asserted-by":"publisher","first-page":"146361","DOI":"10.1016\/j.brainres.2019.146361","volume":"1722","author":"WH Li","year":"2019","unstructured":"Li WH, Cheng X, Yang YL, Liu M, Zhang SS, Wang YH et al (2019) Kaempferol attenuates neuroinflammation and blood brain barrier dysfunction to improve neurological deficits in cerebral ischemia\/reperfusion rats. Brain Res 1722:146361","journal-title":"Brain Res"},{"key":"10134_CR20","doi-asserted-by":"publisher","first-page":"148845","DOI":"10.1016\/j.brainres.2024.148845","volume":"1829","author":"J Luo","year":"2024","unstructured":"Luo J et al (2024) Formononetin alleviates cerebral ischemia-reperfusion injury in rats by targeting the PARP-1\/PARG\/Iduna signaling pathway. Brain Res 1829:148845","journal-title":"Brain Res"},{"issue":"1","key":"10134_CR21","first-page":"8943210","volume":"2018","author":"Y Ma","year":"2018","unstructured":"Ma Y et al (2018) Pinocembrin protects blood-brain barrier function and expands the therapeutic time window for tissue-type plasminogen activator treatment in a rat thromboembolic stroke model. BioMed Res Int 2018(1):8943210","journal-title":"BioMed Res Int"},{"key":"10134_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2202-10-56","volume":"10","author":"A Maddahi","year":"2009","unstructured":"Maddahi A, Chen Q, Edvinsson L (2009) Enhanced cerebrovascular expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 via the MEK\/ERK pathway during cerebral ischemia in the rat. BMC Neurosci 10:1\u201312","journal-title":"BMC Neurosci"},{"issue":"2","key":"10134_CR23","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/s41110-024-00283-6","volume":"49","author":"M Mesgari-Abbasi","year":"2024","unstructured":"Mesgari-Abbasi M, Eskandari Vaezi F, Hosseinzadeh F (2024) Chronic pretreatment of Kombucha tea attenuates post-ischemic brain edema in a transient model of global cerebral ischemia. Nutrire 49(2):40","journal-title":"Nutrire"},{"issue":"1","key":"10134_CR24","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1186\/s12935-024-03519-7","volume":"24","author":"Y Ning","year":"2024","unstructured":"Ning Y et al (2024) RhoA-ROCK2 signaling possesses complex pathophysiological functions in cancer progression and shows promising therapeutic potential. Cancer Cell Int 24(1):339","journal-title":"Cancer Cell Int"},{"issue":"2","key":"10134_CR25","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1179\/1476830514Y.0000000159","volume":"20","author":"M Oz","year":"2017","unstructured":"Oz M et al (2017) 3\u2032, 4\u2032-Dihydroxyflavonol attenuates spatial learning and memory impairments in global cerebral ischemia. Nutr Neurosci 20(2):119\u2013126","journal-title":"Nutr Neurosci"},{"issue":"2","key":"10134_CR27","first-page":"323","volume":"43","author":"LI Shai","year":"2023","unstructured":"Shai LI et al (2023) Soybean isoflavones alleviate cerebral ischemia\/reperfusion injury in rats by inhibiting ferroptosis and inflammatory cascade reaction. J South Med Univ 43(2):323\u2013330","journal-title":"J South Med Univ"},{"key":"10134_CR28","doi-asserted-by":"publisher","first-page":"116500","DOI":"10.1016\/j.biopha.2024.116500","volume":"174","author":"J Shang","year":"2024","unstructured":"Shang J, Jiao J, Wang J, Yan M, Li Q, Shabuerjiang L et al (2024) Chrysin inhibits ferroptosis of cerebral ischemia\/reperfusion injury via regulating HIF-1\u03b1\/CP loop. Biomed Pharmacother 174:116500","journal-title":"Biomed Pharmacother"},{"issue":"12","key":"10134_CR29","doi-asserted-by":"publisher","first-page":"8119","DOI":"10.4049\/jimmunol.0901925","volume":"183","author":"M Steenport","year":"2009","unstructured":"Steenport M et al (2009) Matrix metalloproteinase (MMP)-1 and MMP-3 induce macrophage MMP-9: evidence for the role of TNF-\u03b1 and cyclooxygenase-2. J Immunol 183(12):8119\u20138127","journal-title":"J Immunol"},{"issue":"10","key":"10134_CR30","doi-asserted-by":"publisher","first-page":"973","DOI":"10.3390\/antiox9100973","volume":"9","author":"L Suraweera","year":"2020","unstructured":"Suraweera L et al (2020) Regulation of Nrf2\/ARE pathway by dietary flavonoids: a friend or foe for cancer management? Antioxid (Basel) 9(10):973","journal-title":"Antioxid (Basel)"},{"key":"10134_CR33","doi-asserted-by":"publisher","DOI":"10.2147\/jir.s416239","author":"H-K Wang","year":"2023","unstructured":"Wang H-K et al (2023) HDAC1 is involved in neuroinflammation and blood-brain barrier damage in stroke pathogenesis. J Inflamm Res. https:\/\/doi.org\/10.2147\/jir.s416239","journal-title":"J Inflamm Res"},{"issue":"6","key":"10134_CR32","doi-asserted-by":"publisher","first-page":"1653","DOI":"10.1016\/j.jstrokecerebrovasdis.2018.01.023","volume":"27","author":"L Wang","year":"2018","unstructured":"Wang L et al (2018) The accuracy of serum matrix metalloproteinase-9 for predicting hemorrhagic transformation after acute ischemic stroke: a systematic review and meta-analysis. J Stroke Cerebrovasc Dis 27(6):1653\u20131665","journal-title":"J Stroke Cerebrovasc Dis"},{"key":"10134_CR31","doi-asserted-by":"publisher","first-page":"7028","DOI":"10.1007\/s12035-015-9607-2","volume":"53","author":"W Wang","year":"2016","unstructured":"Wang W et al (2016) GSK-3\u03b2 inhibitor TWS119 attenuates rtPA-induced hemorrhagic transformation and activates the Wnt\/\u03b2-catenin signaling pathway after acute ischemic stroke in rats. Mol Neurobiol 53:7028\u20137036","journal-title":"Mol Neurobiol"},{"key":"10134_CR34","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.phrs.2014.01.004","volume":"81","author":"O Woodman","year":"2014","unstructured":"Woodman O et al (2014) The cardioprotectant 3\u2032, 4\u2032-dihydroxyflavonol inhibits opening of the mitochondrial permeability transition pore after myocardial ischemia and reperfusion in rats. Pharmacol Res 81:26\u201333","journal-title":"Pharmacol Res"},{"issue":"1","key":"10134_CR38","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/s11064-024-04259-w","volume":"50","author":"Y Yang","year":"2025","unstructured":"Yang Y et al (2025) Naringenin protected against blood brain barrier breakdown after ischemic stroke through GSK-3\u03b2\/\u03b2-catenin pathway. Neurochem Res 50(1):17","journal-title":"Neurochem Res"},{"issue":"11","key":"10134_CR36","doi-asserted-by":"publisher","first-page":"3323","DOI":"10.1161\/STROKEAHA.110.608257","volume":"42","author":"Y Yang","year":"2011","unstructured":"Yang Y, Rosenberg GA (2011) Blood\u2013brain barrier breakdown in acute and chronic cerebrovascular disease. Stroke 42(11):3323\u20133328","journal-title":"Stroke"},{"key":"10134_CR37","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.brainres.2015.04.024","volume":"1623","author":"Y Yang","year":"2015","unstructured":"Yang Y, Rosenberg GA (2015) Matrix metalloproteinases as therapeutic targets for stroke. Brain Res 1623:30\u201338","journal-title":"Brain Res"},{"issue":"1","key":"10134_CR39","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1007\/s12017-023-08771-0","volume":"26","author":"E Yilmaz","year":"2024","unstructured":"Yilmaz E et al (2024) Effect of 2-week naringin supplementation on neurogenesis and BDNF levels in ischemia-reperfusion model of rats. Neuromolecular Med 26(1):4","journal-title":"Neuromolecular Med"},{"issue":"6","key":"10134_CR40","doi-asserted-by":"publisher","first-page":"4107","DOI":"10.3892\/etm.2016.3889","volume":"12","author":"Q Zhang","year":"2016","unstructured":"Zhang Q et al (2016) Inhibition of cytoskeletal protein carbonylation may protect against oxidative damage in traumatic brain injury. Exp Ther Med 12(6):4107\u20134112","journal-title":"Exp Ther Med"},{"issue":"1","key":"10134_CR41","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/s00429-021-02404-7","volume":"227","author":"Y Zhang","year":"2022","unstructured":"Zhang Y et al (2022) Different changes in pre-and postsynaptic components in the hippocampal CA1 subfield after transient global cerebral ischemia. Brain Struct Funct 227(1):345\u2013360","journal-title":"Brain Struct Funct"},{"key":"10134_CR42","doi-asserted-by":"publisher","first-page":"1939","DOI":"10.1007\/s11064-016-1904-2","volume":"41","author":"J-J Zhao","year":"2016","unstructured":"Zhao J-J et al (2016) Treatment with isorhamnetin protects the brain against ischemic injury in mice. Neurochem Res 41:1939\u20131948","journal-title":"Neurochem Res"},{"issue":"8","key":"10134_CR43","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1212\/WNL.0000000000004257","volume":"89","author":"C Zhong","year":"2017","unstructured":"Zhong C et al (2017) Serum matrix metalloproteinase-9 levels and prognosis of acute ischemic stroke. Neurology 89(8):805\u2013812","journal-title":"Neurology"},{"issue":"2","key":"10134_CR44","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1016\/j.jep.2011.08.063","volume":"141","author":"H Zhu","year":"2012","unstructured":"Zhu H et al (2012) Baicalin reduces the permeability of the blood\u2013brain barrier during hypoxia in vitro by increasing the expression of tight junction proteins in brain microvascular endothelial cells. J Ethnopharmacol 141(2):714\u2013720","journal-title":"J Ethnopharmacol"}],"container-title":["Journal of Bioenergetics and Biomembranes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10863-026-10134-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10863-026-10134-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10863-026-10134-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T19:02:24Z","timestamp":1788634944000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10863-026-10134-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,5]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["10134"],"URL":"https:\/\/doi.org\/10.1007\/s10863-026-10134-w","relation":{},"ISSN":["0145-479X","1573-6881"],"issn-type":[{"value":"0145-479X","type":"print"},{"value":"1573-6881","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,5]]},"assertion":[{"value":"22 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable.","order":1,"name":"Ethics","label":"Human ethics and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Animal study.","order":2,"name":"Ethics","label":"Consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All authors have approved the publication of this manuscript.","order":3,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","label":"Competing interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":5,"name":"Ethics","label":"Clinical trial number","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"49"}}