{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T18:00:26Z","timestamp":1793642426210,"version":"4.5.1"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["75N91019D00024"],"award-info":[{"award-number":["75N91019D00024"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Clin Immunol"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1007\/s10875-022-01217-5","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T12:08:42Z","timestamp":1648469322000},"page":"986-999","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Interpretation of Dihydrorhodamine-1,2,3 Flow Cytometry in Chronic Granulomatous Disease: an Atypical Exemplar"],"prefix":"10.1007","volume":"42","author":[{"given":"Agnes","family":"Donko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas B.","family":"Kuhns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margot A.","family":"Cousin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith A.","family":"Sacco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric W.","family":"Klee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avni Y.","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralitza H.","family":"Gavrilova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Holland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Leto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshini S.","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,28]]},"reference":[{"issue":"3","key":"1217_CR1","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1097\/00005792-200005000-00004","volume":"79","author":"BH Segal","year":"2000","unstructured":"Segal BH, Leto TL, Gallin JI, Malech HL, Holland SM. Genetic, biochemical, and clinical features of chronic granulomatous disease. Med (Baltim). 2000;79(3):170\u2013200.","journal-title":"Med (Baltim)"},{"issue":"12","key":"1217_CR2","doi-asserted-by":"publisher","first-page":"2543","DOI":"10.1007\/s12325-017-0636-2","volume":"34","author":"DE Arnold","year":"2017","unstructured":"Arnold DE, Heimall JR. A review of chronic granulomatous disease. Adv Ther. 2017;34(12):2543\u201357.","journal-title":"Adv Ther"},{"issue":"1","key":"1217_CR3","doi-asserted-by":"publisher","first-page":"4447","DOI":"10.1038\/s41467-018-06964-x","volume":"9","author":"GA Arnadottir","year":"2018","unstructured":"Arnadottir GA, Norddahl GL, Gudmundsdottir S, Agustsdottir AB, Sigurdsson S, Jensson BO, et al. A homozygous loss-of-function mutation leading to CYBC1 deficiency causes chronic granulomatous disease. Nat Commun. 2018;9(1):4447.","journal-title":"Nat Commun"},{"issue":"2","key":"1217_CR4","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1016\/j.jaci.2018.09.019","volume":"143","author":"DC Thomas","year":"2019","unstructured":"Thomas DC, Charbonnier LM, Schejtman A, Aldhekri H, Coomber EL, Dufficy ER, et al. EROS\/CYBC1 mutations: decreased NADPH oxidase function and chronic granulomatous disease. J Allergy Clin Immunol. 2019;143(2):782-5.e1.","journal-title":"J Allergy Clin Immunol"},{"issue":"5","key":"1217_CR5","first-page":"309","volume":"40","author":"H Berendes","year":"1957","unstructured":"Berendes H, Bridges RA, Good RA. A fatal granulomatosus of childhood: the clinical study of a new syndrome. Minn Med. 1957;40(5):309\u201312.","journal-title":"Minn Med"},{"key":"1217_CR6","doi-asserted-by":"crossref","unstructured":"Holland SM. Chronic granulomatous disease. Hematol Oncol Clin North Am. 2013;27(1):89\u201399, viii.","DOI":"10.1016\/j.hoc.2012.11.002"},{"key":"1217_CR7","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1007\/978-1-4939-9424-3_33","volume":"1982","author":"DB Kuhns","year":"2019","unstructured":"Kuhns DB. Diagnostic testing for chronic granulomatous disease. Methods Mol Biol. 2019;1982:543\u201371.","journal-title":"Methods Mol Biol"},{"issue":"27","key":"1217_CR8","doi-asserted-by":"publisher","first-page":"2600","DOI":"10.1056\/NEJMoa1007097","volume":"363","author":"DB Kuhns","year":"2010","unstructured":"Kuhns DB, Alvord WG, Heller T, Feld JJ, Pike KM, Marciano BE, et al. Residual NADPH oxidase and survival in chronic granulomatous disease. N Engl J Med. 2010;363(27):2600\u201310.","journal-title":"N Engl J Med"},{"issue":"suppl_1","key":"1217_CR9","doi-asserted-by":"publisher","first-page":"S6","DOI":"10.1093\/jpids\/piy007","volume":"7","author":"JE Yu","year":"2018","unstructured":"Yu JE, Azar AE, Chong HJ, Jongco AM 3rd, Prince BT. Considerations in the diagnosis of chronic granulomatous disease. J Pediatric Infect Dis Soc. 2018;7(suppl_1):S6-s11.","journal-title":"J Pediatric Infect Dis Soc"},{"issue":"4","key":"1217_CR10","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1128\/CVI.00001-16","volume":"23","author":"RS Abraham","year":"2016","unstructured":"Abraham RS, Aubert G. Flow cytometry, a versatile tool for diagnosis and monitoring of primary immunodeficiencies. Clin Vaccine Immunol. 2016;23(4):254\u201371.","journal-title":"Clin Vaccine Immunol"},{"issue":"1","key":"1217_CR11","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1007\/s10875-019-00712-6","volume":"40","author":"KA Sacco","year":"2020","unstructured":"Sacco KA, Smith MJ, Bahna SL, Buchbinder D, Burkhardt J, Cooper MA, et al. NAPDH oxidase-specific flow cytometry allows for rapid genetic triage and classification of novel variants in chronic granulomatous disease. J Clin Immunol. 2020;40(1):191\u2013202.","journal-title":"J Clin Immunol"},{"issue":"10","key":"1217_CR12","doi-asserted-by":"publisher","first-page":"3525","DOI":"10.1016\/j.jaip.2020.07.030","volume":"8","author":"HE Baris","year":"2020","unstructured":"Baris HE, Ogulur I, Akcam B, Kiykim A, Karagoz D, Saraymen B, et al. Diagnostic modalities based on flow cytometry for chronic granulomatous disease: a multicenter study in a well-defined cohort. J Allergy Clin Immunol Pract. 2020;8(10):3525-34.e1.","journal-title":"J Allergy Clin Immunol Pract"},{"issue":"2","key":"1217_CR13","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1182\/bloodadvances.2018023184","volume":"3","author":"DB Kuhns","year":"2019","unstructured":"Kuhns DB, Hsu AP, Sun D, Lau K, Fink D, Griffith P, et al. NCF1 (p47(phox))-deficient chronic granulomatous disease: comprehensive genetic and flow cytometric analysis. Blood Adv. 2019;3(2):136\u201347.","journal-title":"Blood Adv"},{"issue":"1","key":"1217_CR14","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1006\/bcmd.2000.0274","volume":"26","author":"SJ Chanock","year":"2000","unstructured":"Chanock SJ, Roesler J, Zhan S, Hopkins P, Lee P, Barrett DT, et al. Genomic structure of the human p47-phox (NCF1) gene. Blood Cells Mol Dis. 2000;26(1):37\u201346.","journal-title":"Blood Cells Mol Dis"},{"issue":"3","key":"1217_CR15","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1172\/JCI113128","volume":"80","author":"CA Parkos","year":"1987","unstructured":"Parkos CA, Allen RA, Cochrane CG, Jesaitis AJ. Purified cytochrome b from human granulocyte plasma membrane is comprised of two polypeptides with relative molecular weights of 91,000 and 22,000. J Clin Invest. 1987;80(3):732\u201342.","journal-title":"J Clin Invest"},{"issue":"1","key":"1217_CR16","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.gene.2016.03.050","volume":"586","author":"MJ Stasia","year":"2016","unstructured":"Stasia MJ. CYBA encoding p22(phox), the cytochrome b558 alpha polypeptide: gene structure, expression, role and physiopathology. Gene. 2016;586(1):27\u201335.","journal-title":"Gene"},{"issue":"6","key":"1217_CR17","doi-asserted-by":"publisher","first-page":"1416","DOI":"10.1182\/blood.V73.6.1416.1416","volume":"73","author":"CA Parkos","year":"1989","unstructured":"Parkos CA, Dinauer MC, Jesaitis AJ, Orkin SH, Curnutte JT. Absence of both the 91kD and 22kD subunits of human neutrophil cytochrome b in two genetic forms of chronic granulomatous disease. Blood. 1989;73(6):1416\u201320.","journal-title":"Blood"},{"key":"1217_CR18","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/978-1-4939-9424-3_9","volume":"1982","author":"MC Dinauer","year":"2019","unstructured":"Dinauer MC. Insights into the NOX NADPH oxidases using heterologous whole cell assays. Methods Mol Biol. 2019;1982:139\u201351.","journal-title":"Methods Mol Biol"},{"issue":"3","key":"1217_CR19","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/s41436-020-01005-9","volume":"23","author":"EW Klee","year":"2021","unstructured":"Klee EW, Cousin MA, Pinto EVF, Morales-Rosado JA, Macke EL, Jenkinson WG, et al. Impact of integrated translational research on clinical exome sequencing. Genet Med. 2021;23(3):498\u2013507.","journal-title":"Genet Med"},{"key":"1217_CR20","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.ymgmr.2017.08.001","volume":"13","author":"FP Vairo","year":"2017","unstructured":"Vairo FP, Boczek NJ, Cousin MA, Kaiwar C, Blackburn PR, Conboy E, et al. The prevalence of diseases caused by lysosome-related genes in a cohort of undiagnosed patients. Mol Genet Metab Rep. 2017;13:46\u201351.","journal-title":"Mol Genet Metab Rep"},{"issue":"4","key":"1217_CR21","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1111\/j.1365-2362.2009.02093.x","volume":"39","author":"MY K\u00f6ker","year":"2009","unstructured":"K\u00f6ker MY, van Leeuwen K, de Boer M, Celmeli F, Metin A, Ozg\u00fcr TT, et al. Six different CYBA mutations including three novel mutations in ten families from Turkey, resulting in autosomal recessive chronic granulomatous disease. Eur J Clin Invest. 2009;39(4):311\u20139.","journal-title":"Eur J Clin Invest"},{"issue":"6108","key":"1217_CR22","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1038\/326088a0","volume":"326","author":"AW Segal","year":"1987","unstructured":"Segal AW. Absence of both cytochrome b-245 subunits from neutrophils in X-linked chronic granulomatous disease. Nat. 1987;326(6108):88\u201391.","journal-title":"Nat"},{"issue":"6","key":"1217_CR23","doi-asserted-by":"publisher","first-page":"1686","DOI":"10.1182\/blood.V73.6.1686.1686","volume":"73","author":"AJ Verhoeven","year":"1989","unstructured":"Verhoeven AJ, Bolscher BG, Meerhof LJ, van Zwieten R, Keijer J, Weening RS, et al. Characterization of two monoclonal antibodies against cytochrome b558 of human neutrophils. Blood. 1989;73(6):1686\u201394.","journal-title":"Blood"},{"issue":"41","key":"1217_CR24","doi-asserted-by":"publisher","first-page":"30336","DOI":"10.1074\/jbc.M607191200","volume":"281","author":"Y Zhu","year":"2006","unstructured":"Zhu Y, Marchal CC, Casbon AJ, Stull N, von L\u00f6hneysen K, Knaus UG, et al. Deletion mutagenesis of p22phox subunit of flavocytochrome b558: identification of regions critical for gp91phox maturation and NADPH oxidase activity. J Biol Chem. 2006;281(41):30336\u201346.","journal-title":"J Biol Chem"},{"issue":"10","key":"1217_CR25","doi-asserted-by":"publisher","first-page":"8421","DOI":"10.1074\/jbc.M109778200","volume":"277","author":"I Dahan","year":"2002","unstructured":"Dahan I, Issaeva I, Gorzalczany Y, Sigal N, Hirshberg M, Pick E. Mapping of functional domains in the p22(phox) subunit of flavocytochrome b(559) participating in the assembly of the NADPH oxidase complex by \u201cpeptide walking.\u201d J Biol Chem. 2002;277(10):8421\u201332.","journal-title":"J Biol Chem"},{"issue":"44","key":"1217_CR26","doi-asserted-by":"publisher","first-page":"45935","DOI":"10.1074\/jbc.M406486200","volume":"279","author":"RK Ambasta","year":"2004","unstructured":"Ambasta RK, Kumar P, Griendling KK, Schmidt HH, Busse R, Brandes RP. Direct interaction of the novel Nox proteins with p22phox is required for the formation of a functionally active NADPH oxidase. J Biol Chem. 2004;279(44):45935\u201341.","journal-title":"J Biol Chem"},{"issue":"1","key":"1217_CR27","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1042\/BJ20060819","volume":"403","author":"Y Nakano","year":"2007","unstructured":"Nakano Y, Banfi B, Jesaitis AJ, Dinauer MC, Allen LA, Nauseef WM. Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Biochem J. 2007;403(1):97\u2013108.","journal-title":"Biochem J"},{"issue":"4","key":"1217_CR28","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1128\/MCB.01393-09","volume":"30","author":"K von L\u00f6hneysen","year":"2010","unstructured":"von L\u00f6hneysen K, Noack D, Wood MR, Friedman JS, Knaus UG. Structural insights into Nox4 and Nox2: motifs involved in function and cellular localization. Mol Cell Biol. 2010;30(4):961\u201375.","journal-title":"Mol Cell Biol"},{"key":"1217_CR29","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.freeradbiomed.2017.12.031","volume":"116","author":"M Zana","year":"2018","unstructured":"Zana M, P\u00e9terfi Z, Kov\u00e1cs HA, T\u00f3th ZE, Enyedi B, Morel F, et al. Interaction between p22(phox) and Nox4 in the endoplasmic reticulum suggests a unique mechanism of NADPH oxidase complex formation. Free Radic Biol Med. 2018;116:41\u20139.","journal-title":"Free Radic Biol Med"},{"issue":"24","key":"1217_CR30","doi-asserted-by":"publisher","first-page":"4307","DOI":"10.1093\/bioinformatics\/bty536","volume":"34","author":"P Zhang","year":"2018","unstructured":"Zhang P, Bigio B, Rapaport F, Zhang SY, Casanova JL, Abel L, et al. PopViz: a webserver for visualizing minor allele frequencies and damage prediction scores of human genetic variations. Bioinforma. 2018;34(24):4307\u20139.","journal-title":"Bioinforma"},{"issue":"9","key":"1217_CR31","doi-asserted-by":"publisher","first-page":"3957","DOI":"10.1172\/JCI97116","volume":"128","author":"A van de Geer","year":"2018","unstructured":"van de Geer A, Nieto-Patl\u00e1n A, Kuhns DB, Tool AT, Arias AA, Bouaziz M, et al. Inherited p40phox deficiency differs from classic chronic granulomatous disease. J Clin Invest. 2018;128(9):3957\u201375.","journal-title":"J Clin Invest"},{"issue":"1","key":"1217_CR32","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/S0022-3476(96)70437-7","volume":"128","author":"SJ Vowells","year":"1996","unstructured":"Vowells SJ, Fleisher TA, Sekhsaria S, Alling DW, Maguire TE, Malech HL. Genotype-dependent variability in flow cytometric evaluation of reduced nicotinamide adenine dinucleotide phosphate oxidase function in patients with chronic granulomatous disease. J Pediatr. 1996;128(1):104\u20137.","journal-title":"J Pediatr"},{"key":"1217_CR33","doi-asserted-by":"publisher","first-page":"102596","DOI":"10.1016\/j.bcmd.2021.102596","volume":"92","author":"D Roos","year":"2021","unstructured":"Roos D, van Leeuwen K, Hsu AP, Priel DL, Begtrup A, Brandon R, et al. Hematologically important mutations: the autosomal forms of chronic granulomatous disease (third update). Blood Cells Mol Dis. 2021;92:102596.","journal-title":"Blood Cells Mol Dis"},{"issue":"6","key":"1217_CR34","doi-asserted-by":"publisher","first-page":"2329","DOI":"10.1084\/jem.180.6.2329","volume":"180","author":"JH Leusen","year":"1994","unstructured":"Leusen JH, Bolscher BG, Hilarius PM, Weening RS, Kaulfersch W, Seger RA, et al. 156Pro\u2013>Gln substitution in the light chain of cytochrome b558 of the human NADPH oxidase (p22-phox) leads to defective translocation of the cytosolic proteins p47-phox and p67-phox. J Exp Med. 1994;180(6):2329\u201334.","journal-title":"J Exp Med"},{"issue":"22","key":"1217_CR35","doi-asserted-by":"publisher","first-page":"10650","DOI":"10.1073\/pnas.91.22.10650","volume":"91","author":"TL Leto","year":"1994","unstructured":"Leto TL, Adams AG, de Mendez I. Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets. Proc Natl Acad Sci U S A. 1994;91(22):10650\u20134.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"36","key":"1217_CR36","doi-asserted-by":"publisher","first-page":"22152","DOI":"10.1074\/jbc.271.36.22152","volume":"271","author":"H Sumimoto","year":"1996","unstructured":"Sumimoto H, Hata K, Mizuki K, Ito T, Kage Y, Sakaki Y, et al. Assembly and activation of the phagocyte NADPH oxidase. Specific interaction of the N-terminal Src homology 3 domain of p47phox with p22phox is required for activation of the NADPH oxidase. J Biol Chem. 1996;271(36):22152\u20138.","journal-title":"J Biol Chem"},{"issue":"5","key":"1217_CR37","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1111\/j.1365-3083.2012.02771.x","volume":"76","author":"MA Jakobsen","year":"2012","unstructured":"Jakobsen MA, Katzenstein TL, Valerius NH, Roos D, Fisker N, Mogensen TH, et al. Genetical analysis of all Danish patients diagnosed with chronic granulomatous disease. Scand J Immunol. 2012;76(5):505\u201311.","journal-title":"Scand J Immunol"},{"issue":"5","key":"1217_CR38","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.1016\/j.jaci.2013.05.039","volume":"132","author":"MY K\u00f6ker","year":"2013","unstructured":"K\u00f6ker MY, Camc\u0131o\u011flu Y, van Leeuwen K, K\u0131l\u0131\u00e7 S, Barlan I, Y\u0131lmaz M, et al. Clinical, functional, and genetic characterization of chronic granulomatous disease in 89 Turkish patients. J Allergy Clin Immunol. 2013;132(5):1156-63.e5.","journal-title":"J Allergy Clin Immunol"},{"issue":"6","key":"1217_CR39","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1111\/j.1365-2141.2008.07148.x","volume":"141","author":"S Teimourian","year":"2008","unstructured":"Teimourian S, Zomorodian E, Badalzadeh M, Pouya A, Kannengiesser C, Mansouri D, et al. Characterization of six novel mutations in CYBA: the gene causing autosomal recessive chronic granulomatous disease. Br J Haematol. 2008;141(6):848\u201351.","journal-title":"Br J Haematol"},{"issue":"1","key":"1217_CR40","doi-asserted-by":"publisher","first-page":"e84251","DOI":"10.1371\/journal.pone.0084251","volume":"9","author":"Q Xu","year":"2014","unstructured":"Xu Q, Yuan F, Shen X, Wen H, Li W, Cheng B, et al. Polymorphisms of C242T and A640G in CYBA gene and the risk of coronary artery disease: a meta-analysis. PLoS One. 2014;9(1):e84251.","journal-title":"PLoS One."}],"container-title":["Journal of Clinical Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10875-022-01217-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10875-022-01217-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10875-022-01217-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:03:14Z","timestamp":1661385794000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10875-022-01217-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,28]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["1217"],"URL":"https:\/\/doi.org\/10.1007\/s10875-022-01217-5","relation":{},"ISSN":["0271-9142","1573-2592"],"issn-type":[{"value":"0271-9142","type":"print"},{"value":"1573-2592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,28]]},"assertion":[{"value":"21 September 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 January 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 March 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The patient and parents were consented to a research protocol approved by the Institutional Review Board (IRB # 1942\u201305 and 1831\u201305) of the Mayo Clinic, Rochester, MN.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclaimer"}}]}}