{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T16:36:31Z","timestamp":1777307791745,"version":"3.51.4"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100015613","name":"Centre Hospitalier Universitaire de Lille","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100015613","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Clin Immunol"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    can be responsible for severe infections, especially in patients with primary antibody deficiencies like selective anti-polysaccharide antibodies deficiency (SPAD). The reference method recommaned by the World Health Organization for assessment of anti-pneumococcal capsular polysaccharides (PCPs) IgG antibodies is a standardized serotype-specific ELISA (WHO-SSA), but this manual method is time-consuming and limit the number of evaluated PCPs. We aim to evaluate the performance values of a multiplex assay based on electrochemiluminescence (ECL-plex). A panel of 164 sera from 82 patients sampled before and 4\u20138 weeks after immunization by the 23-valent pneumococcal polysaccharide vaccine (PPV23) were assessed by the reference WHO-SSA (7 to 13 serotypes) and by an 18-plex ECL assay (18 serotypes). All patients had normal serum Ig\/subclasses levels and were classified as good (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200943) or poor responders (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200939, i.e. SPAD patients) according to the American Academy of Asthma, Allergy and Immunology\u2019s (AAAAI) current guidelines. We observed excellent correlations between the two methods for anti-PCPs titers against 7 serotypes (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.88 [95% CI: 0.87\u20130.90],\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009124 sera) and 13 serotypes (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.87 [0.87\u20130.89],\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200940 sera). Using the AAAAI\u2019s guidelines for interpretation, the test performance of the 18-plex ECL assay for SPAD diagnosis showed a sensitivity of 95% and specificity of 84%, positive and negative predictive values of 84% and 95%, respectively. The percentage of agreement was 89% between the SSA and the 18-plex ECL assay. The 18-plex ECL assay is a reliable, rapid, and simple method for evaluating anti-PCPs response and screening for SPAD diagnosis.\n                  <\/jats:p>","DOI":"10.1007\/s10875-025-01911-0","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T10:11:37Z","timestamp":1752142297000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a Multiplex Electrochemiluminescence Assay for Detection of Anti-Pneumococcal Antibodies in the Diagnosis of Selective Polysaccharide Antibody Deficiency"],"prefix":"10.1007","volume":"45","author":[{"given":"Nicolas","family":"Perrard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurore","family":"Collet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Stabler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Poizot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam","family":"Labalette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gatien","family":"Durand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Batteux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Floriane","family":"Mirgot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvain","family":"Dubucquoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Chevrier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillaume","family":"Lef\u00e8vre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,10]]},"reference":[{"key":"1911_CR1","doi-asserted-by":"publisher","first-page":"a010215","DOI":"10.1101\/cshperspect.a010215","volume":"3","author":"B Henriques-Normark","year":"2013","unstructured":"Henriques-Normark B, Tuomanen EI. The pneumococcus: epidemiology, microbiology, and pathogenesis. Cold Spring Harb Perspect Med. 2013;3:a010215.","journal-title":"Cold Spring Harb Perspect Med"},{"key":"1911_CR2","unstructured":"Batah J, Varon E. Rapport d\u2019activit\u00e9 2017. 2017."},{"key":"1911_CR3","doi-asserted-by":"publisher","first-page":"2674","DOI":"10.3389\/fimmu.2018.02674","volume":"9","author":"M Sadarangani","year":"2018","unstructured":"Sadarangani M. Protection against invasive infections in children caused by encapsulated Bacteria. Front Immunol. 2018;9:2674.","journal-title":"Front Immunol"},{"key":"1911_CR4","doi-asserted-by":"publisher","unstructured":"Geno KA, Gilbert GL, Song JY, Skovsted IC, Klugman KP, Jones C et al. Pneumococcal Capsules and Their Types: Past, Present, and Future. 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Collet reports travel grant from Grifols, outside the submitted work.G. Lef\u00e8vre received consulting fees for advisory boards from Grifols, Biotest, and Takeda; personal fees for meetings from LFB, Octapharma, and Grifols; and research funding from LFB, CSL Behring, Octapharma, Takeda, Vitalaire, and Biotest.The rest of the authors declare they have no relevant conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"112"}}