{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T12:24:40Z","timestamp":1789820680933,"version":"4.0.1"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2013,11,28]],"date-time":"2013-11-28T00:00:00Z","timestamp":1385596800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Fluoresc"],"published-print":{"date-parts":[[2014,3]]},"DOI":"10.1007\/s10895-013-1329-0","type":"journal-article","created":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T07:28:29Z","timestamp":1385537309000},"page":"313-318","source":"Crossref","is-referenced-by-count":28,"title":["Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging"],"prefix":"10.1007","volume":"24","author":[{"given":"Linying","family":"Cao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingfeng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,11,28]]},"reference":[{"key":"1329_CR1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0891-5849(99)00226-9","volume":"28","author":"S Schuchmann","year":"2000","unstructured":"Schuchmann S, Heinemann U (2000) Increased mitochondrial superoxide generation in neurons from trisomy 16 mice: a model of Down\u2019s syndrome. Free Radic Biol Med 28:235\u2013250","journal-title":"Free Radic Biol Med"},{"key":"1329_CR2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.pbb.2012.02.011","volume":"101","author":"K Parameshwaran","year":"2012","unstructured":"Parameshwaran K, Irwin MH, Steliou K, Pinkert CA (2012) Protection by an antioxidant of rotenone-induced neuromotor decline, reactive oxygen species generation and cellular stress in mouse brain. Pharmacol, Biochem Behav 101:487\u2013492","journal-title":"Pharmacol, Biochem Behav"},{"key":"1329_CR3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0027-5107(99)00004-4","volume":"424","author":"J Cadet","year":"1999","unstructured":"Cadet J, Delatour T, Douki T, Gasparutto D, Pouget JP, Ravanat JL, Sauvaigo S (1999) Hydroxyl radicals and DNA base damage. Mutat Res, Fundam Mol Mech Mutagen 424:9\u201321","journal-title":"Mutat Res, Fundam Mol Mech Mutagen"},{"key":"1329_CR4","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1007\/s00216-006-0366-9","volume":"386","author":"N Soh","year":"2006","unstructured":"Soh N (2006) Recent advances in fluorescent probes for the detection of reactive oxygen species. Anal Bioanal Chem 386:532\u2013543","journal-title":"Anal Bioanal Chem"},{"key":"1329_CR5","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1016\/S0021-9258(19)39850-3","volume":"265","author":"BE Britigan","year":"1990","unstructured":"Britigan BE, Coffman TJ, Buettner GR (1990) Spin trapping evidence for the lack of significant hydroxyl radical production during the respiration burst of human phagocytes using a spin adduct resistant to superoxide-mediated destruction. J Biol Chem 265:2650\u20132656","journal-title":"J Biol Chem"},{"key":"1329_CR6","doi-asserted-by":"crossref","first-page":"8307","DOI":"10.1016\/S0021-9258(18)42443-X","volume":"267","author":"CL Ramos","year":"1992","unstructured":"Ramos CL, Pou S, Britigan BE, Cohen MS, Rosen GM (1992) Spin trapping evidence for myeloperoxidase-dependent hydroxyl radical formation by human neutrophils and monocytes. J Biol Chem 267:8307\u20138312","journal-title":"J Biol Chem"},{"key":"1329_CR7","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1002\/bmc.339","volume":"18","author":"LX Li","year":"2004","unstructured":"Li LX, Abe Y, Nagasawa Y, Kudo R, Usui N, Imai K, Mashino T, Mochizuki M, Miyata N (2004) An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid. Biomed Chromatogr 18:470\u2013474","journal-title":"Biomed Chromatogr"},{"key":"1329_CR8","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1007\/s00216-006-0395-4","volume":"385","author":"D Pezo","year":"2006","unstructured":"Pezo D, Salafranca J, Nerin C (2006) Design of a method for generation of gas-phase hydroxyl radicals, and use of HPLC with fluorescence detection to assess the antioxidant capacity of natural essential oils. Anal Bioanal Chem 385:1241\u20131246","journal-title":"Anal Bioanal Chem"},{"key":"1329_CR9","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1002\/chem.200700455","volume":"14","author":"H Tang","year":"2008","unstructured":"Tang H, Zhang N, Chen ZZ, Xu KH, Zhuo LG, An LG, Yang GW (2008) Probing hydroxyl radicals and their imaging in living cells by use of FAM-DNA-Au nanoparticles. Chem Eur J 14:522\u2013528","journal-title":"Chem Eur J"},{"key":"1329_CR10","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1039\/B314204C","volume":"5","author":"N Soh","year":"2004","unstructured":"Soh N, Makihara K, Sakoda E, Imato T (2004) A ratiometric fluorescent probe for imaging hydroxyl radicals in living cells. Chem Commun 5:496\u2013497","journal-title":"Chem Commun"},{"key":"1329_CR11","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1039\/b103208a","volume":"126","author":"XF Yang","year":"2001","unstructured":"Yang XF, Guo XQ (2001) Investigation of the anthracene-nitroxide hybrid molecule as a probe for hydroxyl radicals. Analyst (Cambridge, U K) 126:1800\u20131804","journal-title":"Analyst (Cambridge, U K)"},{"key":"1329_CR12","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1002\/chem.200901514","volume":"16","author":"P Li","year":"2010","unstructured":"Li P, Xie T, Duan X, Yu FB, Wang X, Tang B (2010) A new highly selective and sensitive assay for fluorescence imaging of.OH in living cells: effectively avoiding the interference of peroxynitrite. Chem Eur J 16:1834\u20131840","journal-title":"Chem Eur J"},{"key":"1329_CR13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1021\/ol202816m","volume":"14","author":"NB Yapici","year":"2012","unstructured":"Yapici NB, Jockusch S, Moscatelli A, Mandalapu SR, Itagaki Y, Bates DK, Wiseman S, Gibson KM, Turro NJ, Bi LR (2012) New rhodamine nitroxide based fluorescent probes for intracellular hydroxyl radical identification in living cells. Org Lett 14:50\u201353","journal-title":"Org Lett"},{"key":"1329_CR14","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.aca.2009.02.044","volume":"639","author":"T Maki","year":"2009","unstructured":"Maki T, Soh N, Fukaminato T, Nakajima H, Nakano K, Imato T (2009) Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals. Anal Chim Acta 639:78\u201382","journal-title":"Anal Chim Acta"},{"key":"1329_CR15","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1021\/ja00214a041","volume":"110","author":"NV Blough","year":"1988","unstructured":"Blough NV, Simpson DJ (1988) Chemically mediated fluorescence yield switching in nitroxide fluorophore adducts\u2014optical sensors of radical redox reactions. J Am Chem Soc 110:1915\u20131917","journal-title":"J Am Chem Soc"},{"key":"1329_CR16","doi-asserted-by":"crossref","first-page":"4721","DOI":"10.1021\/ja210315x","volume":"134","author":"HY Ahn","year":"2012","unstructured":"Ahn HY, Fairfull-Smith KE, Morrow BJ, Lussini V, Kim B, Bondar MV, Bottle SE, Belfield KD (2012) Two-photon fluorescence microscopy imaging of cellular oxidative stress using profluorescent nitroxides. J Am Chem Soc 134:4721\u20134730","journal-title":"J Am Chem Soc"},{"key":"1329_CR17","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1016\/j.freeradbiomed.2010.09.009","volume":"49","author":"M Ikeda","year":"2010","unstructured":"Ikeda M, Nakagawa H, Ban S, Tsumoto H, Suzuki T, Miyata N (2010) Development of a DNA-binding TEMPO derivative for evaluation of nuclear oxidative stress and its application in living cells. Free Radic Biol Med 49:1792\u20131797","journal-title":"Free Radic Biol Med"},{"key":"1329_CR18","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1016\/j.bmcl.2007.01.011","volume":"17","author":"S Ban","year":"2007","unstructured":"Ban S, Nakagawa H, Suzuki T, Miyata N (2007) Novel mitochondria-localizing TEMPO derivative for measurement of cellular oxidative stress in mitochondria. Bioorg Med Chem Lett 17:2055\u20132058","journal-title":"Bioorg Med Chem Lett"},{"key":"1329_CR19","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s10895-012-1067-8","volume":"22","author":"QP Zuo","year":"2012","unstructured":"Zuo QP, Li ZJ, Hu YH, Li B, Huang LH, Wang CJ, Liu SK, Liao HQ (2012) A highly sensitive fluorescent probe for HClO and its application in live cell imaging. J Fluoresc 22:1201\u20131207","journal-title":"J Fluoresc"},{"key":"1329_CR20","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1007\/s10895-012-1065-x","volume":"22","author":"FY Yan","year":"2012","unstructured":"Yan FY, Cao DL, Wang M, Yang N, Yu QH, Dai LF, Chen L (2012) A new rhodamine-based \u201coff-on\u201d fluorescent chemosensor for Hg (II) ion and its application in imaging Hg (II) in living cells. J Fluoresc 22:1249\u20131256","journal-title":"J Fluoresc"},{"key":"1329_CR21","doi-asserted-by":"crossref","first-page":"587","DOI":"10.3109\/10715761003709802","volume":"44","author":"XP Chen","year":"2010","unstructured":"Chen XP, Zhong ZF, Xu ZT, Chen LD, Wang YT (2010) 2\u2032, 7 \u2032-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy. Free Radical Res 44:587\u2013604","journal-title":"Free Radical Res"},{"key":"1329_CR22","doi-asserted-by":"crossref","first-page":"16188","DOI":"10.1074\/jbc.274.23.16188","volume":"274","author":"A Barrientos","year":"1999","unstructured":"Barrientos A, Moraes CT (1999) Titrating the effects of mitochondrial complex I impairment in the cell physiology. J Biol Chem 274:16188\u201316197","journal-title":"J Biol Chem"},{"key":"1329_CR23","doi-asserted-by":"crossref","first-page":"8516","DOI":"10.1074\/jbc.M210432200","volume":"278","author":"NY Li","year":"2003","unstructured":"Li NY, Ragheb K, Lawler G, Sturgist J, Rajwa B, Melendez JA, Robinson JP (2003) Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem 278:8516\u20138525","journal-title":"J Biol Chem"},{"key":"1329_CR24","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.mito.2006.11.026","volume":"7","author":"HP Indo","year":"2007","unstructured":"Indo HP, Davidson M, Yen HC, Suenaga S, Tomita K, Nishii T, Higuchi M, Koga Y, Ozawa T, Majima HJ (2007) Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage. Mitochondrion 7:106\u2013118","journal-title":"Mitochondrion"},{"key":"1329_CR25","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/10715769900300991","volume":"31","author":"M Saran","year":"1999","unstructured":"Saran M, Summer KH (1999) Assaying for hydroxyl radicals: Hydroxylated terephthalate is a superior fluorescence marker than hydroxylated benzoate. Free Radical Res 31:429\u2013436","journal-title":"Free Radical Res"},{"key":"1329_CR26","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1006\/abio.2000.4958","volume":"290","author":"R Biondi","year":"2001","unstructured":"Biondi R, Xia Y, Rossi R, Paolocci N, Ambrosio G, Zweier JL (2001) Detection of hydroxyl radicals by D-phenylalanine hydroxylation: a specific assay for hydroxyl radical generation in biological systems. Anal Biochem 290:138\u2013145","journal-title":"Anal Biochem"},{"key":"1329_CR27","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1021\/ja00811a062","volume":"96","author":"JW Reed","year":"1974","unstructured":"Reed JW, Ho HH, Jolly WL (1974) Chemical syntheses with a quenched flow reactor- hydroxytrihydroborate and peroxynitrite. J Am Chem Soc 96:1248\u20131249","journal-title":"J Am Chem Soc"}],"container-title":["Journal of Fluorescence"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10895-013-1329-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10895-013-1329-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10895-013-1329-0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T19:21:08Z","timestamp":1647544868000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10895-013-1329-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,28]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["1329"],"URL":"https:\/\/doi.org\/10.1007\/s10895-013-1329-0","relation":{},"ISSN":["1053-0509","1573-4994"],"issn-type":[{"value":"1053-0509","type":"print"},{"value":"1573-4994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,28]]}}}