{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:57:36Z","timestamp":1793159856571,"version":"4.5.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"the \u201cPioneer\u201d and \u201cLeading Goose\u201d R&D Program of Zhejiang","award":["(No. 2022C03118\uff0c2023C03075"],"award-info":[{"award-number":["(No. 2022C03118\uff0c2023C03075"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No. 82172336"],"award-info":[{"award-number":["No. 82172336"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Natural Science Foundation of Zhejiang Province","award":["LQ23H050005"],"award-info":[{"award-number":["LQ23H050005"]}]},{"name":"the Scientific research project of Zhejiang Provincial Education Department","award":["Y202250731"],"award-info":[{"award-number":["Y202250731"]}]},{"name":"Wuxi \"Taihu talent plan\" for excellent medical expert team","award":["No. 2021-9"],"award-info":[{"award-number":["No. 2021-9"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Fluoresc"],"DOI":"10.1007\/s10895-024-03917-0","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T08:04:29Z","timestamp":1725523469000},"page":"5355-5366","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Establishment of trypsinogen-2 Amplification Luminescent Proximity Homogeneous Assay and its Application in Acute Pancreatitis"],"prefix":"10.1007","volume":"35","author":[{"given":"Meichun","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongming","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifan","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"issue":"5","key":"3917_CR1","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1016\/j.pan.2020.06.005","volume":"20","author":"AL Yang","year":"2020","unstructured":"Yang AL, McNabb-Baltar J (2020) Hypertriglyceridemia and acute pancreatitis. Pancreatology 20(5):795\u2013800","journal-title":"Pancreatology"},{"issue":"2","key":"3917_CR2","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.pan.2017.01.005","volume":"17","author":"SE Roberts","year":"2017","unstructured":"Roberts SE et al (2017) The incidence and aetiology of acute pancreatitis across Europe. Pancreatology 17(2):155\u2013165","journal-title":"Pancreatology"},{"issue":"4","key":"3917_CR3","first-page":"382","volume":"325","author":"MA Mederos","year":"2021","unstructured":"Mederos MA, Reber HA, Girgis MD (2021) Acute Pancreatitis: Rev Jama 325(4):382\u2013390","journal-title":"Acute Pancreatitis: Rev Jama"},{"issue":"20","key":"3917_CR4","doi-asserted-by":"publisher","first-page":"1972","DOI":"10.1056\/NEJMra1505202","volume":"375","author":"CE Forsmark","year":"2016","unstructured":"Forsmark CE, Vege SS, Wilcox CM (2016) Acute Pancreat N Engl J Med 375(20):1972\u20131981","journal-title":"Acute Pancreat N Engl J Med"},{"issue":"12","key":"3917_CR5","doi-asserted-by":"publisher","first-page":"1251","DOI":"10.1007\/s40265-022-01766-4","volume":"82","author":"P Szatmary","year":"2022","unstructured":"Szatmary P et al (2022) Acute Pancreatitis: diagnosis and treatment. Drugs 82(12):1251\u20131276","journal-title":"Drugs"},{"issue":"5","key":"3917_CR6","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1097\/MOG.0000000000000456","volume":"34","author":"TW James","year":"2018","unstructured":"James TW, Crockett SD (2018) Management of acute pancreatitis in the first 72 hours. Curr Opin Gastroenterol 34(5):330\u2013335","journal-title":"Curr Opin Gastroenterol"},{"issue":"8","key":"3917_CR7","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1038\/s41575-019-0158-2","volume":"16","author":"PJ Lee","year":"2019","unstructured":"Lee PJ, Papachristou GI (2019) New insights into acute pancreatitis. Nat Rev Gastroenterol Hepatol 16(8):479\u2013496","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"11","key":"3917_CR8","doi-asserted-by":"publisher","first-page":"1216","DOI":"10.1080\/003655200750056727","volume":"35","author":"E Kemppainen","year":"2000","unstructured":"Kemppainen E et al (2000) Time course profile of serum trypsinogen-2 and trypsin-2-alpha1-antitrypsin in patients with acute pancreatitis. Scand J Gastroenterol 35(11):1216\u20131220","journal-title":"Scand J Gastroenterol"},{"issue":"2","key":"3917_CR9","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1093\/clinchem\/39.2.234","volume":"39","author":"EC Wong","year":"1993","unstructured":"Wong EC, Butch AW, Rosenblum JL (1993) The clinical chemistry laboratory and acute pancreatitis. Clin Chem 39(2):234\u2013243","journal-title":"Clin Chem"},{"issue":"2","key":"3917_CR10","doi-asserted-by":"publisher","first-page":"136","DOI":"10.3109\/00365511003615317","volume":"70","author":"O Itkonen","year":"2010","unstructured":"Itkonen O (2010) Human trypsinogens in the pancreas and in cancer. Scand J Clin Lab Invest 70(2):136\u2013143","journal-title":"Scand J Clin Lab Invest"},{"issue":"2","key":"3917_CR11","doi-asserted-by":"publisher","first-page":"69","DOI":"10.3109\/10408363.2015.1075468","volume":"53","author":"UH Stenman","year":"2016","unstructured":"Stenman UH (2016) Biomarker development, from bench to bedside. Crit Rev Clin Lab Sci 53(2):69\u201386","journal-title":"Crit Rev Clin Lab Sci"},{"issue":"2","key":"3917_CR12","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1080\/00365510151097584","volume":"61","author":"J Hedstr\u00f6m","year":"2001","unstructured":"Hedstr\u00f6m J et al (2001) Trypsinogen-1, -2 and tumour-associated trypsin-inhibitor in bile and biliary tract tissues from patients with biliary tract diseases and pancreatic carcinomas. Scand J Clin Lab Invest 61(2):111\u2013118","journal-title":"Scand J Clin Lab Invest"},{"issue":"2","key":"3917_CR13","first-page":"424","volume":"96","author":"J Hedstr\u00f6m","year":"2001","unstructured":"Hedstr\u00f6m J et al (2001) A comparison of serum trypsinogen-2 and trypsin-2-alpha1-antitrypsin complex with lipase and amylase in the diagnosis and assessment of severity in the early phase of acute pancreatitis. Am J Gastroenterol 96(2):424\u2013430","journal-title":"Am J Gastroenterol"},{"issue":"4","key":"3917_CR14","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1136\/jcp.2002.002923","volume":"59","author":"WR Matull","year":"2006","unstructured":"Matull WR, Pereira SP (2006) O\u2019Donohue, biochemical markers of acute pancreatitis. J Clin Pathol 59(4):340\u2013344","journal-title":"J Clin Pathol"},{"issue":"3","key":"3917_CR15","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1097\/MPA.0000000000001260","volume":"48","author":"M Rainio","year":"2019","unstructured":"Rainio M et al (2019) Serum serine peptidase inhibitor Kazal-Type 1, Trypsinogens 1 to 3, and Complex of Trypsin 2 and \u03b11-Antitrypsin in the diagnosis of severe Acute Pancreatitis. Pancreas 48(3):374\u2013380","journal-title":"Pancreas"},{"key":"3917_CR16","doi-asserted-by":"publisher","first-page":"112215","DOI":"10.1016\/j.bios.2020.112215","volume":"160","author":"M El-Maghrabey","year":"2020","unstructured":"El-Maghrabey M et al (2020) Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling. Biosens Bioelectron 160:112215","journal-title":"Biosens Bioelectron"},{"key":"3917_CR17","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.cca.2022.08.020","volume":"535","author":"Z Xiang","year":"2022","unstructured":"Xiang Z et al (2022) Development and application of a novel aldehyde nanoparticle-based amplified luminescent proximity homogeneous assay for rapid quantitation of pancreatic stone protein. Clin Chim Acta 535:120\u2013130","journal-title":"Clin Chim Acta"},{"key":"3917_CR18","doi-asserted-by":"publisher","first-page":"113487","DOI":"10.1016\/j.jim.2023.113487","volume":"517","author":"J Chen","year":"2023","unstructured":"Chen J et al (2023) Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA242. J Immunol Methods 517:113487","journal-title":"J Immunol Methods"},{"issue":"4","key":"3917_CR19","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1007\/s10895-022-02950-1","volume":"32","author":"X Chen","year":"2022","unstructured":"Chen X et al (2022) Establishment and clinical application of a highly sensitive time-resolved fluorescence immunoassay for Tumor-Associated Trypsinogen-2. J Fluoresc 32(4):1501\u20131507","journal-title":"J Fluoresc"},{"issue":"2","key":"3917_CR20","doi-asserted-by":"publisher","first-page":"188","DOI":"10.5152\/tjg.2018.18137","volume":"30","author":"M Lin","year":"2019","unstructured":"Lin M et al (2019) Level of serum soluble Tim-3 expression in early-phase acute pancreatitis. Turk J Gastroenterol 30(2):188\u2013191","journal-title":"Turk J Gastroenterol"},{"issue":"10","key":"3917_CR21","doi-asserted-by":"publisher","first-page":"2317","DOI":"10.1093\/cvr\/cvab282","volume":"118","author":"M Jovani","year":"2022","unstructured":"Jovani M et al (2022) Cardiovascular disease related circulating biomarkers and cancer incidence and mortality: is there an association? Cardiovasc Res 118(10):2317\u20132328","journal-title":"Cardiovasc Res"},{"issue":"11","key":"3917_CR22","doi-asserted-by":"publisher","first-page":"1675","DOI":"10.3748\/wjg.v10.i11.1675","volume":"10","author":"JT Jiang","year":"2004","unstructured":"Jiang JT et al (2004) Serum level of TSGF, CA242 and CA19-9 in pancreatic cancer. World J Gastroenterol 10(11):1675\u20131677","journal-title":"World J Gastroenterol"},{"issue":"11","key":"3917_CR23","doi-asserted-by":"publisher","first-page":"2024","DOI":"10.1136\/gutjnl-2016-313595","volume":"66","author":"SM van Dijk","year":"2017","unstructured":"van Dijk SM et al (2017) Acute pancreatitis: recent advances through randomised trials. Gut 66(11):2024\u20132032","journal-title":"Gut"},{"issue":"10252","key":"3917_CR24","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1016\/S0140-6736(20)31310-6","volume":"396","author":"L Boxhoorn","year":"2020","unstructured":"Boxhoorn L et al (2020) Acute pancreatitis. Lancet 396(10252):726\u2013734","journal-title":"Lancet"},{"issue":"18","key":"3917_CR25","doi-asserted-by":"publisher","first-page":"1275","DOI":"10.1016\/j.clinbiochem.2017.07.003","volume":"50","author":"OZ Ismail","year":"2017","unstructured":"Ismail OZ, Bhayana V (2017) Lipase or amylase for the diagnosis of acute pancreatitis? Clin Biochem 50(18):1275\u20131280","journal-title":"Clin Biochem"},{"issue":"8","key":"3917_CR26","doi-asserted-by":"publisher","first-page":"818","DOI":"10.3109\/00365529609010359","volume":"31","author":"V Sainio","year":"1996","unstructured":"Sainio V et al (1996) Serum trypsinogen-2 in the prediction of outcome in acute necrotizing pancreatitis. Scand J Gastroenterol 31(8):818\u2013824","journal-title":"Scand J Gastroenterol"},{"key":"3917_CR27","doi-asserted-by":"crossref","unstructured":"Rompianesi G et al (2017) Serum amylase and lipase and urinary trypsinogen and amylase for diagnosis of acute pancreatitis. Cochrane Database Syst Rev, 4(4): p. Cd012010.","DOI":"10.1002\/14651858.CD012010.pub2"},{"issue":"9","key":"3917_CR28","doi-asserted-by":"publisher","first-page":"1761","DOI":"10.1093\/clinchem\/40.9.1761","volume":"40","author":"J Hedstr\u00f6m","year":"1994","unstructured":"Hedstr\u00f6m J et al (1994) Time-resolved immunofluorometric assay of trypsin-2 complexed with alpha 1-antitrypsin in serum. Clin Chem 40(9):1761\u20131765","journal-title":"Clin Chem"},{"issue":"1","key":"3917_CR29","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/0009-8981(89)90254-4","volume":"184","author":"M Kimland","year":"1989","unstructured":"Kimland M et al (1989) Immunoreactive anionic and cationic trypsin in human serum. Clin Chim Acta 184(1):31\u201346","journal-title":"Clin Chim Acta"},{"issue":"138","key":"3917_CR30","first-page":"435","volume":"62","author":"J Cao","year":"2015","unstructured":"Cao J et al (2015) Correlations between serum trypsinogen-2 and pancreatic cancer. Hepatogastroenterology 62(138):435\u2013440","journal-title":"Hepatogastroenterology"},{"issue":"3","key":"3917_CR31","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1002\/(SICI)1097-0215(19960503)66:3<326::AID-IJC10>3.0.CO;2-9","volume":"66","author":"J Hedstr\u00f6m","year":"1996","unstructured":"Hedstr\u00f6m J et al (1996) Serum trypsinogen-2 and trypsin-2-alpha(1)-antitrypsin complex in malignant and benign digestive-tract diseases. Preferential elevation in patients with cholangiocarcinomas. Int J Cancer 66(3):326\u2013331","journal-title":"Int J Cancer"},{"issue":"5","key":"3917_CR32","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.jhep.2007.05.017","volume":"47","author":"M Lempinen","year":"2007","unstructured":"Lempinen M et al (2007) Enhanced detection of cholangiocarcinoma with serum trypsinogen-2 in patients with severe bile duct strictures. J Hepatol 47(5):677\u2013683","journal-title":"J Hepatol"},{"issue":"6","key":"3917_CR33","doi-asserted-by":"publisher","first-page":"e23248","DOI":"10.1002\/jcla.23248","volume":"34","author":"M Chen","year":"2020","unstructured":"Chen M et al (2020) Soluble Tim3 detection by time-resolved fluorescence immunoassay and its application in membranous nephropathy. J Clin Lab Anal 34(6):e23248","journal-title":"J Clin Lab Anal"},{"issue":"12","key":"3917_CR34","doi-asserted-by":"publisher","first-page":"e23508","DOI":"10.1002\/jcla.23508","volume":"34","author":"B Huang","year":"2020","unstructured":"Huang B et al (2020) Receptor antibody time-resolved A2 phospholipase bead immunochromatography and its application in idiopathic membranous nephropathy. J Clin Lab Anal 34(12):e23508","journal-title":"J Clin Lab Anal"},{"key":"3917_CR35","doi-asserted-by":"publisher","first-page":"115016","DOI":"10.1016\/j.ab.2022.115016","volume":"662","author":"Z Xiang","year":"2023","unstructured":"Xiang Z et al (2023) Development of amplified luminescent proximity homogeneous assay for quantitation of gastrin-17. Anal Biochem 662:115016","journal-title":"Anal Biochem"},{"issue":"5","key":"3917_CR36","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1136\/gut.41.5.690","volume":"41","author":"E Kemppainen","year":"1997","unstructured":"Kemppainen E et al (1997) Increased serum trypsinogen 2 and trypsin 2-alpha 1 antitrypsin complex values identify endoscopic retrograde cholangiopancreatography induced pancreatitis with high accuracy. Gut 41(5):690\u2013695","journal-title":"Gut"},{"key":"3917_CR37","doi-asserted-by":"publisher","first-page":"114906","DOI":"10.1016\/j.ab.2022.114906","volume":"657","author":"Z Xiang","year":"2022","unstructured":"Xiang Z et al (2022) Development and application of amplified luminescent proximity homogeneous assay for quantitation of heparin-binding protein. Anal Biochem 657:114906","journal-title":"Anal Biochem"},{"issue":"1","key":"3917_CR38","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1136\/gutjnl-2012-302779","volume":"62","author":"PA Banks","year":"2013","unstructured":"Banks PA et al (2013) Classification of acute pancreatitis\u20132012: revision of the Atlanta classification and definitions by international consensus. Gut 62(1):102\u2013111","journal-title":"Gut"}],"container-title":["Journal of Fluorescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10895-024-03917-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10895-024-03917-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10895-024-03917-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T22:05:01Z","timestamp":1757109901000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10895-024-03917-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,5]]},"references-count":38,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["3917"],"URL":"https:\/\/doi.org\/10.1007\/s10895-024-03917-0","relation":{},"ISSN":["1573-4994"],"issn-type":[{"value":"1573-4994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,5]]},"assertion":[{"value":"7 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 August 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was conducted in accordance with the ethical guidelines of the Declaration of Helsinki as it relates to human experimentation and was approved by the Ethics Committee of the Jiangyin People\u2019s Hospital.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Patients signed informed consent regarding publishing their data and photographs.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}