{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:28:54Z","timestamp":1758630534867},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2014,11,19]],"date-time":"2014-11-19T00:00:00Z","timestamp":1416355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2014,11,19]],"date-time":"2014-11-19T00:00:00Z","timestamp":1416355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1007\/s10930-014-9588-4","type":"journal-article","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T11:17:00Z","timestamp":1416568620000},"page":"557-564","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Role of Neurotoxin Associated Proteins in the Low pH Induced Structural Changes in the Botulinum Neurotoxin Complex"],"prefix":"10.1007","volume":"33","author":[{"given":"Gowri","family":"Chellappan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raj","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuowei","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bal Ram","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,11,19]]},"reference":[{"key":"9588_CR1","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1002\/(SICI)1097-4598(199806)21:6<701::AID-MUS1>3.0.CO;2-B","volume":"21","author":"M Cherington","year":"1998","unstructured":"Cherington M (1998) Clinical spectrum of botulism. Muscle Nerve 21:701\u2013710","journal-title":"Muscle Nerve"},{"key":"9588_CR2","first-page":"55","volume":"195","author":"CL Hatheway","year":"1995","unstructured":"Hatheway CL (1995) Botulism: the present status of the disease. Curr Top Microbiol Immunol 195:55\u201375","journal-title":"Curr Top Microbiol Immunol"},{"key":"9588_CR3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/mr.46.1.86-94.1982","volume":"46","author":"DM Gill","year":"1982","unstructured":"Gill DM (1982) Bacterial toxins: a table of lethal amounts. Microbiol Rev 46:86\u201394","journal-title":"Microbiol Rev"},{"key":"9588_CR4","doi-asserted-by":"publisher","first-page":"24","DOI":"10.3390\/toxins2010024","volume":"2","author":"P Capek","year":"2010","unstructured":"Capek P, Dickerson TJ (2010) Sensing the deadliest toxin: technologies for botulinum neurotoxin detection. Toxins (Basel) 2:24\u201353","journal-title":"Toxins (Basel)"},{"key":"9588_CR5","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1128\/iai.16.1.107-109.1977","volume":"16","author":"I Ohishi","year":"1977","unstructured":"Ohishi I, Sugii S, Sakaguchi G (1977) Oral toxicities of Clostridium botulinum toxins in response to molecular size. Infect Immun 16:107\u2013109","journal-title":"Infect Immun"},{"key":"9588_CR6","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/0163-7258(82)90061-4","volume":"19","author":"G Sakaguchi","year":"1982","unstructured":"Sakaguchi G (1982) Clostridium botulinum toxins. Pharmacol Ther 19:165\u2013194","journal-title":"Pharmacol Ther"},{"key":"9588_CR7","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1128\/iai.64.5.1589-1594.1996","volume":"64","author":"K Inoue","year":"1996","unstructured":"Inoue K, Fujinaga Y, Watanabe T, Ohyama T, Takeshi K et al (1996) Molecular composition of Clostridium botulinum type A progenitor toxins. Infect Immun 64:1589\u20131594","journal-title":"Infect Immun"},{"key":"9588_CR8","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1128\/iai.16.3.910-914.1977","volume":"16","author":"S Sugii","year":"1977","unstructured":"Sugii S, Ohishi I, Sakaguchi G (1977) Correlation between oral toxicity and in vitro stability of Clostridium botulinum type A and B toxins of different molecular sizes. Infect Immun 16:910\u2013914","journal-title":"Infect Immun"},{"key":"9588_CR9","doi-asserted-by":"publisher","first-page":"1529","DOI":"10.1099\/mic.0.26805-0","volume":"150","author":"Y Fujinaga","year":"2004","unstructured":"Fujinaga Y, Inoue K, Watarai S, Sakaguchi Y, Arimitsu H et al (2004) Molecular characterization of binding subcomponents of Clostridium botulinum type C progenitor toxin for intestinal epithelial cells and erythrocytes. Microbiology 150:1529\u20131538","journal-title":"Microbiology"},{"key":"9588_CR10","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1016\/j.toxicon.2008.11.014","volume":"54","author":"Y Fujinaga","year":"2009","unstructured":"Fujinaga Y, Matsumura T, Jin Y, Takegahara Y, Sugawara Y (2009) A novel function of botulinum toxin-associated proteins: HA proteins disrupt intestinal epithelial barrier to increase toxin absorption. Toxicon 54:583\u2013586","journal-title":"Toxicon"},{"key":"9588_CR11","first-page":"21","volume":"364","author":"S Gu","year":"2013","unstructured":"Gu S, Jin R (2013) Assembly and function of the botulinum neurotoxin progenitor complex. Curr Top Microbiol Immunol 364:21\u201344","journal-title":"Curr Top Microbiol Immunol"},{"key":"9588_CR12","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1021\/mp0601244","volume":"4","author":"DT Brandau","year":"2007","unstructured":"Brandau DT, Joshi SB, Smalter AM, Kim S, Steadman B et al (2007) Stability of the Clostridium botulinum type A neurotoxin complex: an empirical phase diagram based approach. Mol Pharm 4:571\u2013582","journal-title":"Mol Pharm"},{"key":"9588_CR13","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1016\/0041-0101(84)90085-0","volume":"22","author":"BR DasGupta","year":"1984","unstructured":"DasGupta BR, Sathyamoorthy V (1984) Purification and amino acid composition of type A botulinum neurotoxin. Toxicon 22:415\u2013424","journal-title":"Toxicon"},{"key":"9588_CR14","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/0076-6879(86)30013-2","volume":"130","author":"JT Yang","year":"1986","unstructured":"Yang JT, Wu CS, Martinez HM (1986) Calculation of protein conformation from circular dichroism. Methods Enzymol 130:208\u2013269","journal-title":"Methods Enzymol"},{"key":"9588_CR15","doi-asserted-by":"publisher","first-page":"14733","DOI":"10.1021\/ja106167d","volume":"132","author":"JS Prell","year":"2010","unstructured":"Prell JS, Correra TC, Chang TM, Biles JA, Williams ER (2010) Entropy drives an attached water molecule from the C- to N-terminus on protonated proline. J Am Chem Soc 132:14733\u201314735","journal-title":"J Am Chem Soc"},{"key":"9588_CR16","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.bbapap.2005.06.005","volume":"1751","author":"SM Kelly","year":"2005","unstructured":"Kelly SM, Jess TJ, Price NC (2005) How to study proteins by circular dichroism. Biochim Biophys Acta 1751:119\u2013139","journal-title":"Biochim Biophys Acta"},{"key":"9588_CR17","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1023\/A:1022590514771","volume":"17","author":"F-N Fu","year":"1998","unstructured":"Fu F-N, Sharma S, Singh B (1998) A protease-resistant novel hemagglutinin purified from type A Clostridium botulinum. J Protein Chem 17:53","journal-title":"J Protein Chem"},{"key":"9588_CR18","first-page":"27","volume":"145","author":"CJ Malizio","year":"2000","unstructured":"Malizio CJ, Goodnough MC, Johnson EA (2000) Purification of Clostridium botulinum type A neurotoxin. Methods Mol Biol 145:27\u201339","journal-title":"Methods Mol Biol"},{"key":"9588_CR19","doi-asserted-by":"crossref","first-page":"10461","DOI":"10.1016\/S0021-9258(19)85105-0","volume":"260","author":"V Sathyamoorthy","year":"1985","unstructured":"Sathyamoorthy V, DasGupta BR (1985) Separation, purification, partial characterization and comparison of the heavy and light chains of botulinum neurotoxin types A, B, and E. J Biol Chem 260:10461\u201310466","journal-title":"J Biol Chem"},{"key":"9588_CR20","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1007\/s10930-013-9465-6","volume":"32","author":"AM Bryant","year":"2013","unstructured":"Bryant AM, Davis J, Cai S, Singh BR (2013) Molecular composition and extinction coefficient of native botulinum neurotoxin complex produced by Clostridium botulinum hall A strain. Protein J 32:106\u2013117","journal-title":"Protein J"},{"key":"9588_CR21","first-page":"239","volume":"7","author":"SK Sharma","year":"1998","unstructured":"Sharma SK, Singh BR (1998) Hemagglutinin binding mediated protection of botulinum neurotoxin from proteolysis. J Nat Toxins 7:239\u2013253","journal-title":"J Nat Toxins"},{"key":"9588_CR22","doi-asserted-by":"publisher","first-page":"6903","DOI":"10.1021\/bi990086c","volume":"38","author":"S Cai","year":"1999","unstructured":"Cai S, Sarkar HK, Singh BR (1999) Enhancement of the endopeptidase activity of botulinum neurotoxin by its associated proteins and dithiothreitol. Biochemistry 38:6903\u20136910","journal-title":"Biochemistry"},{"key":"9588_CR23","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1124\/jpet.104.066845","volume":"310","author":"AB Maksymowych","year":"2004","unstructured":"Maksymowych AB, Simpson LL (2004) Structural features of the botulinum neurotoxin molecule that govern binding and transcytosis across polarized human intestinal epithelial cells. J Pharmacol Exp Ther 310:633\u2013641","journal-title":"J Pharmacol Exp Ther"},{"key":"9588_CR24","doi-asserted-by":"publisher","first-page":"e1003690","DOI":"10.1371\/journal.ppat.1003690","volume":"9","author":"K Lee","year":"2013","unstructured":"Lee K, Gu S, Jin L, Le TT, Cheng LW (2013) Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity. PLoS Pathog 9:e1003690","journal-title":"PLoS Pathog"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-014-9588-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-014-9588-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-014-9588-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-014-9588-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:12:54Z","timestamp":1667520774000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-014-9588-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,19]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["9588"],"URL":"https:\/\/doi.org\/10.1007\/s10930-014-9588-4","relation":{},"ISSN":["1572-3887","1875-8355"],"issn-type":[{"value":"1572-3887","type":"print"},{"value":"1875-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,19]]},"assertion":[{"value":"19 November 2014","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}