{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T02:43:49Z","timestamp":1772678629221,"version":"3.50.1"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T00:00:00Z","timestamp":1465948800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T00:00:00Z","timestamp":1465948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"published-print":{"date-parts":[[2016,8]]},"DOI":"10.1007\/s10930-016-9669-7","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T12:39:06Z","timestamp":1465994346000},"page":"256-268","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cloning, Expression, and Characterization of Siamese Crocodile (Crocodylus siamensis) Hemoglobin from Escherichia coli and Pichia pastoris"],"prefix":"10.1007","volume":"35","author":[{"given":"Preeyanan","family":"Anwised","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nisachon","family":"Jangpromma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theeranan","family":"Temsiripong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rina","family":"Patramanon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakda","family":"Daduang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarawut","family":"Jitrapakdee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Araki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sompong","family":"Klaynongsruang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,6,15]]},"reference":[{"key":"9669_CR1","doi-asserted-by":"publisher","first-page":"2091","DOI":"10.1016\/S0006-3495(95)80388-7","volume":"68","author":"AB Anderson","year":"1995","unstructured":"Anderson AB, Robertson CR (1995) Absorption spectra indicate conformational alteration of myoglobin adsorbed on polydimethylsiloxane. Biophys J 68:2091\u20132097","journal-title":"Biophys J"},{"issue":"2","key":"9669_CR2","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1152\/ajpheart.00037.2003","volume":"285","author":"KM Bobofchak","year":"2003","unstructured":"Bobofchak KM, Mito T, Texel SJ, Bellelli A, Nemoto M, Traystman RJ, Koehler RC, Brinigar WS, Fronticelli C (2003) A recombinant polymeric hemoglobin with conformational, functional, and physiological characteristics of an in vivo O2 transporter. Am J Physiol Heart Circ Physiol 285(2):549\u2013561","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"1","key":"9669_CR3","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1006\/bbrc.1993.1778","volume":"194","author":"S Cociancich","year":"1993","unstructured":"Cociancich S, Goyffon M, Bontems F, Bulet P, Bouet F, Menez A, Hoffmann JA (1993) Purification and characterization of a Scorpion defensin, a 4\u00a0kDa antibacterial peptide presenting structural similarities with insect defensins and Scorpion toxins. Biochem Biophys Res Commun 194(1):17\u201322","journal-title":"Biochem Biophys Res Commun"},{"issue":"36","key":"9669_CR4","doi-asserted-by":"publisher","first-page":"25330","DOI":"10.1074\/jbc.274.36.25330","volume":"274","author":"AC Fogaca","year":"1999","unstructured":"Fogaca AC, da Silva PJ, Miranda MT, Bianchi AG, Miranda A, Ribolla PE, Daffre S (1999) Antimicrobial activity of a bovine hemoglobin fragment in the Tick Boophilus microplus. J Biol Chem 274(36):25330\u201325334","journal-title":"J Biol Chem"},{"key":"9669_CR5","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/j.peptides.2008.01.011","volume":"29","author":"N Nedjar-Arroume","year":"2008","unstructured":"Nedjar-Arroume N, Dubois-Delval V, Adje EY, Traisnel J, Krier F, Mary P, Kouach M, Briand G, Guillochon D (2008) Bovine hemoglobin: an attractive source of antibacterial peptide. Peptides 29:969\u2013977","journal-title":"Peptides"},{"issue":"3","key":"9669_CR6","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.jsgi.2006.02.009","volume":"13","author":"P Mak","year":"2006","unstructured":"Mak P, Wicherek L, Suder P, Dubin A, Banas T, Kaim I, Klimek M (2006) Analysis of free hemoglobin level and hemoglobin peptides from human puerperal uterine secretions. J Soc Gynecol Investig 13(3):285\u2013291","journal-title":"J Soc Gynecol Investig"},{"key":"9669_CR7","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1093\/humrep\/den318","volume":"24","author":"L Deng","year":"2009","unstructured":"Deng L, Pan X, Wang Y, Wang L, Zhou XE, Li M, Feng Y, Wu Q, Wang B, Huang N (2009) Hemoglobin and its derived peptides may play a role in the antibacterial mechanism of the vagina. Hum Reprod 24:211\u2013218","journal-title":"Hum Reprod"},{"key":"9669_CR8","unstructured":"Hoffman B, Key B, Ofer B, Kiryat T (2002) Reptilian-derived peptides for the treatment of microbial infections. In (United States) US 6,340,667"},{"key":"9669_CR9","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/s10930-011-9372-7","volume":"31","author":"J Jandaruang","year":"2012","unstructured":"Jandaruang J, Siritapetawee J, Thumanu K, Songsiriritthigul C, Krittanai C, Daduang S, Dhiravisit A, Thammasirirak S (2012) The Effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobin. Protein J 31:43\u201350","journal-title":"Protein J"},{"key":"9669_CR10","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1007\/s10930-012-9424-7","volume":"31","author":"S Srihongthong","year":"2012","unstructured":"Srihongthong S, Pakdeesuwan A, Daduang S, Araki T, Dhiravisit A, Thammasirirak S (2012) Complete amino acid sequence of globin chains and biological activity of fragmented crocodile hemoglobin (Crocodylus siamensis). Protein J 31:466\u2013476","journal-title":"Protein J"},{"key":"9669_CR11","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1007\/s10930-014-9581-y","volume":"33","author":"S Phosri","year":"2014","unstructured":"Phosri S, Mahakunakorn P, Lueangsakulthai J, Jangpromma N, Swatsitang P, Daduang S, Dhiravisit A, Thammasirirak S (2014) An investigation of antioxidant and anti-inflammatory activities from blood components of Crocodile (Crocodylus siamensis). Protein J 33:484\u2013492","journal-title":"Protein J"},{"issue":"3","key":"9669_CR12","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1007\/s10930-013-9474-5","volume":"32","author":"P Anwised","year":"2013","unstructured":"Anwised P, Kabbua T, Temsiripong T, Dhiravisit A, Jitrapakdee S, Araki T, Yoneda K, Thammasirirak S (2013) Molecular cloning and expression of \u03b1-globin and \u03b2-globin genes from crocodile (Crocodylus siamensis). Protein J 32(3):172\u2013182","journal-title":"Protein J"},{"key":"9669_CR13","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1007\/s10930-014-9569-7","volume":"33","author":"J Jandaruang","year":"2014","unstructured":"Jandaruang J, Siritapetawee J, Songsiriritthigul C, Preecharram S, Azuma T, Dhiravisit A, Fukumori Y, Thammasirirak S (2014) Purification, characterization, and crystallization of Crocodylus siamensis hemoglobin. Protein J 33:377\u2013385","journal-title":"Protein J"},{"key":"9669_CR14","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1139\/b95-336","volume":"73","author":"JM Cregg","year":"1995","unstructured":"Cregg JM, Higgins DR (1995) Production of foreign proteins in the yeast Pichia pastoris. Can J Bot 73:891\u2013897","journal-title":"Can J Bot"},{"key":"9669_CR15","doi-asserted-by":"publisher","first-page":"3859","DOI":"10.1093\/nar\/15.9.3859","volume":"15","author":"JE Tschopp","year":"1987","unstructured":"Tschopp JE, Brust PE, Cregg JM, Stillman CA, Gingeras GR (1987) Expression of the LacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res 15:3859\u20133876","journal-title":"Nucleic Acids Res"},{"key":"9669_CR16","first-page":"48","volume":"12","author":"KA Barr","year":"1992","unstructured":"Barr KA, Hopkins SA, Sreekrishna K (1992) Protocol for efficient secretion of HSA developed from Pichia pastoris. Pharm Eng 12:48\u201351","journal-title":"Pharm Eng"},{"key":"9669_CR17","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1006\/abbi.2001.2564","volume":"395","author":"MS Almeida","year":"2001","unstructured":"Almeida MS, Cabral KS, Medeiros L, Valente FCL, Almeida FCL, Kurtenbach E (2001) cDNA cloning and heterologous expression of functional cysteine-rich antifugal protein Psd 1 in the yeast Pichia pastoris. Arch Biochem Biophys 395:199\u2013207","journal-title":"Arch Biochem Biophys"},{"key":"9669_CR18","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/S1046-5928(03)00136-0","volume":"31","author":"KMS Cabral","year":"2003","unstructured":"Cabral KMS, Almeida MS, Valente AP, Almeida FCL, Kurtenbach E (2003) Production of the active antifugual Pisum sativum defensin 1(Psd1) in Pichia pastoris: overcoming the inefficiency of the STE13 protease. Protein Expr Purif 31:115\u2013122","journal-title":"Protein Expr Purif"},{"key":"9669_CR19","doi-asserted-by":"publisher","first-page":"2256","DOI":"10.1021\/jf030662i","volume":"52","author":"JJ Chen","year":"2004","unstructured":"Chen JJ, Chen GH, Hsu HC, Li SS, Chen CS (2004) Cloning and functional expression of a mungbean defensin VrD1 in Pichia pastoris. J Agric Food Chem 52:2256\u20132261","journal-title":"J Agric Food Chem"},{"key":"9669_CR20","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.pep.2004.09.006","volume":"39","author":"L Li","year":"2005","unstructured":"Li L, Wang JX, Zhao XF, Kang CJ, Liu N, Xiang JH, Li FH, Sueda FHS, Kondo H (2005) High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris. Protein Expr Purif 39:144\u2013151","journal-title":"Protein Expr Purif"},{"issue":"6","key":"9669_CR21","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1093\/nar\/7.6.1513","volume":"7","author":"HC Birnboim","year":"1979","unstructured":"Birnboim HC, Doly J (1979) A rapid alkaline extraction prodedure for screening recombinant plasmid DNA. Nucleic Acids Res 7(6):1513\u20131523","journal-title":"Nucleic Acids Res"},{"key":"9669_CR22","doi-asserted-by":"publisher","first-page":"10337","DOI":"10.1021\/bi00200a014","volume":"33","author":"SL Alam","year":"1994","unstructured":"Alam SL, Dutton DP, Satterlee JD (1994) Expression of recombinant monomer hemoglobins (Component IV) from the marine annelied Glycera dibranchiata: evidence for primary sequence positional regulation of heme rotational disorder. Biochemistry 33:10337\u201310344","journal-title":"Biochemistry"},{"issue":"32","key":"9669_CR23","doi-asserted-by":"publisher","first-page":"29600","DOI":"10.1074\/jbc.M211832200","volume":"278","author":"KI Oinuma","year":"2003","unstructured":"Oinuma KI, Hashimoto Y, Konishi K, Goda M, Noguchi T, Higashibata H, Kobayashi K (2003) Novel aldoxime dehydratase involved in carbon-nitrogen triple bond synthesis of Pseudomonas chlororaphis B23. J Biol Chem 278(32):29600\u201329608","journal-title":"J Biol Chem"},{"issue":"1","key":"9669_CR24","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0021-9258(19)44469-4","volume":"248","author":"LM Siegel","year":"1973","unstructured":"Siegel LM, Murphy MJ, Kamin H (1973) Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. I. The Escherichia coli hemoflavoprotein: molecular parameters and prosthetic groups. J Biol Chem 248(1):251\u2013264","journal-title":"J Biol Chem"},{"key":"9669_CR25","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/S0005-2728(96)00164-8","volume":"1319","author":"SU Metzger","year":"1997","unstructured":"Metzger SU, Cramer WA, Whitmarsh J (1997) Critical analysis of the extinction coefficient of chloroplast cytochrome f. Biochim Biophys Acta 1319:233\u2013241","journal-title":"Biochim Biophys Acta"},{"issue":"1","key":"9669_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0003-2697(87)90643-9","volume":"161","author":"EA Berry","year":"1987","unstructured":"Berry EA, Trumpower BL (1987) Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra. Anal Biochem 161(1):1\u201315","journal-title":"Anal Biochem"},{"key":"9669_CR27","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.pep.2014.08.013","volume":"103","author":"T Kabbua","year":"2014","unstructured":"Kabbua T, Anwised P, Boonmee A, Subedi BP, Pierce BS, Thammasirirak S (2014) Autoinduction, purification, and characterization of soluble \u03b1-globin chains of crocodile (Crocodylus siamensis) hemoglobin in Escherichia coli. Protein Expr Purif 103:56\u201363","journal-title":"Protein Expr Purif"},{"key":"9669_CR28","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-3-11","author":"LF Vallejo","year":"2004","unstructured":"Vallejo LF, Rinas U (2004) Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins. Microb Cell Fact. doi:10.1186\/1475-2859-3-11","journal-title":"Microb Cell Fact"},{"key":"9669_CR29","doi-asserted-by":"publisher","first-page":"1852","DOI":"10.1126\/science.1068408","volume":"295","author":"FU Hartl","year":"2002","unstructured":"Hartl FU, Hayer-Hartl M (2002) Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 295:1852\u20131858","journal-title":"Science"},{"key":"9669_CR30","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/S2221-1691(11)60213-X","volume":"2","author":"O Khow","year":"2012","unstructured":"Khow O, Suntrarachun S (2012) Strategies for production of active eukaryotic proteins in bacterial expression system. Asian Pac J Trop Biomed 2:159\u2013162","journal-title":"Asian Pac J Trop Biomed"},{"key":"9669_CR31","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1007\/BF00284196","volume":"243","author":"H Ohi","year":"1994","unstructured":"Ohi H, Miura M, Hiramatsu R, Ohmura T (1994) The positive and negative cis-acting elements for methanol regulation in the Pichia pastoris AOX2 gene. Mol Genet Genomics 243:489\u2013499","journal-title":"Mol Genet Genomics"},{"key":"9669_CR32","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1016\/0958-1669(95)80087-5","volume":"6","author":"MA Romanos","year":"1995","unstructured":"Romanos MA (1995) Advances in the use of Pichia pastoris for high-level gene expression. Curr Opin Biotechnol 6:527\u2013533","journal-title":"Curr Opin Biotechnol"},{"key":"9669_CR33","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.tibtech.2004.04.006","volume":"22","author":"C Gustafsson","year":"2004","unstructured":"Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein expression. Trends Biotechnol 22:346\u2013353","journal-title":"Trends Biotechnol"},{"key":"9669_CR34","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.pep.2008.03.017","volume":"60","author":"H Wang","year":"2008","unstructured":"Wang H, Wang Q, Zhang F, Huang Y, Ji Y, Hou Y (2008) Protein expression and purification of human Zbtb7A in Pichia pastoris via gene codon optimization and synthesis. Protein Expr Purif 60:97\u2013102","journal-title":"Protein Expr Purif"},{"key":"9669_CR35","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1007\/s10529-011-0790-6","volume":"34","author":"Z Gao","year":"2012","unstructured":"Gao Z, Li Z, Zhang Y, Huang H, Li M, Zhou L, Tang Y, Yao B, Zhang W (2012) High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization. Biotechnol Lett 34:507\u2013514","journal-title":"Biotechnol Lett"},{"key":"9669_CR36","doi-asserted-by":"publisher","DOI":"10.1038\/srep03043","author":"P Yu","year":"2013","unstructured":"Yu P, Yan Y, Gu Q, Wang X (2013) Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris. Sci Rep. doi:10.1038\/srep03043","journal-title":"Sci Rep"},{"key":"9669_CR37","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.pep.2015.09.028","volume":"118","author":"S Maijaroen","year":"2016","unstructured":"Maijaroen S, Anwised P, Klaynongsruang S, Daduang S, Boonmee A (2016) Comparison of recombinant & #x03B1;-hemoglobin from Crocodylus siamensis expressed in different cloning vectors and their biological properties. Protein Expr Purif 118:55\u201363","journal-title":"Protein Expr Purif"},{"key":"9669_CR38","doi-asserted-by":"crossref","first-page":"7160","DOI":"10.1016\/S0021-9258(17)38369-2","volume":"261","author":"LO Narhi","year":"1986","unstructured":"Narhi LO, Fulco AJ (1986) Characterization of a Catalytically Self-sufficient 119,000-Dalton Cytochrome P-450 Monooxygenase Induced by Barbiturates in Bacillus megaterium. J Biol Chem 261:7160\u20137169","journal-title":"J Biol Chem"},{"issue":"4","key":"9669_CR39","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1021\/bi991598u","volume":"39","author":"Y Kato","year":"2000","unstructured":"Kato Y, Nakamura K, Sakiyama H, Mayhew SG, Asano Y (2000) Novel heme-containing lyase, phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1: purification, characterization, and molecular cloning of the gene. Biochemistry 39(4):800\u2013809","journal-title":"Biochemistry"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-016-9669-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-016-9669-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-016-9669-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-016-9669-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:15:43Z","timestamp":1667520943000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-016-9669-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,15]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["9669"],"URL":"https:\/\/doi.org\/10.1007\/s10930-016-9669-7","relation":{},"ISSN":["1572-3887","1875-8355"],"issn-type":[{"value":"1572-3887","type":"print"},{"value":"1875-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,15]]},"assertion":[{"value":"15 June 2016","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}