{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T04:09:02Z","timestamp":1744258142463,"version":"3.40.4"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T00:00:00Z","timestamp":1546387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T00:00:00Z","timestamp":1546387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"published-print":{"date-parts":[[2019,2,15]]},"DOI":"10.1007\/s10930-018-09808-1","type":"journal-article","created":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T08:39:32Z","timestamp":1546418372000},"page":"50-57","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Trans-sialidase Protein as a Potential Serological Marker for African Trypanosomiasis"],"prefix":"10.1007","volume":"38","author":[{"given":"Ana Filipa","family":"Teixeira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Gomes","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f3nia","family":"Pestana-Ascens\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1000-0149","authenticated-orcid":false,"given":"Marcelo Sousa","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,1,2]]},"reference":[{"issue":"1","key":"9808_CR1","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.pt.2003.10.016","volume":"20","author":"KR Matthews","year":"2004","unstructured":"Matthews KR, Ellis JR, Paterou A (2004) Molecular regulation of the life cycle of African trypanosomes. Trends Parasitol 20(1):40\u201347","journal-title":"Trends Parasitol"},{"issue":"5906","key":"9808_CR2","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/302349a0","volume":"302","author":"ML Cardoso de Almeida","year":"1983","unstructured":"Cardoso de Almeida ML, Turner MJ (1983) The membrane form of variant surface glycoproteins of Trypanosoma brucei. Nature 302(5906):349\u2013352","journal-title":"Nature"},{"issue":"2126","key":"9808_CR3","first-page":"1","volume":"7","author":"A Ponte-Sucre","year":"2016","unstructured":"Ponte-Sucre A (2016) An overview of Trypanosoma brucei infections: an intense host-parasite interaction. Front Microbiol 7(2126):1\u201312","journal-title":"Front Microbiol"},{"issue":"45","key":"9808_CR4","doi-asserted-by":"publisher","first-page":"33949","DOI":"10.1074\/jbc.M604951200","volume":"281","author":"GN Montagna","year":"2006","unstructured":"Montagna GN, Donelson JE, Frasch AC (2006) Procyclic Trypanosoma brucei expresses separate sialidase and trans-sialidase enzymes on its surface membrane. J Biol Chem 281(45):33949\u201333958","journal-title":"J Biol Chem"},{"issue":"12","key":"9808_CR5","doi-asserted-by":"publisher","first-page":"2941","DOI":"10.1046\/j.1432-1033.2002.02968.x","volume":"269","author":"G Montagna","year":"2002","unstructured":"Montagna G, Cremona ML, Paris G, Amaya MF, Buschiazzo A, Alzari PM, Frasch AC (2002) The trans-sialidase from the African trypanosome Trypanosoma brucei. Eur J Biochem 269(12):2941\u20132950","journal-title":"Eur J Biochem"},{"issue":"1","key":"9808_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0166-6851(93)90153-O","volume":"61","author":"M Engstler","year":"1993","unstructured":"Engstler M, Reuter G, Schauer R (1993) The developmentally regulated trans-sialidase from Trypanosoma brucei sialylates the procyclic acidic repetitive protein. Mol Biochem Parasitol 61(1):1\u201313","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"9808_CR7","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1084\/jem.177.2.465","volume":"177","author":"LC de PontesCarvalho","year":"1993","unstructured":"Pontes de Carvalho, LC, Tomlinson S, Vandekerckhove F, Bienen EJ, Clarkson AB, Jiang MS, Hart GW, Nussenzweig V (1993) Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor. J Exp Med 177(2):465\u2013474","journal-title":"J Exp Med"},{"issue":"7","key":"9808_CR8","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1016\/0092-8674(91)90008-M","volume":"65","author":"S Schenkman","year":"1991","unstructured":"Schenkman S, Jiang MS, Hart GW, Nussenzweig V (1991) A novel cell surface trans-sialidase of Trypanosoma cruzi generates a stage-specific epitope required for invasion of mammalian cells. Cell 65(7):1117\u20131125","journal-title":"Cell"},{"issue":"6","key":"9808_CR9","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1016\/0169-4758(93)90017-A","volume":"9","author":"S Schankman","year":"1993","unstructured":"Schankman S, Eichinger D (1993) Trypanosoma cruzi trans-sialidase and cell invasion. Parasitol Today 9(6):218\u2013222","journal-title":"Parasitol Today"},{"issue":"2","key":"9808_CR10","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/0166-6851(96)02582-0","volume":"77","author":"CM Egima","year":"1996","unstructured":"Egima CM, Briones MR, Freitas J\u00fanior LH, Schenkman RP, Uemura H, Schenkman S (1996) Organizaton of trans-sialidase genes in Trypanosoma cruzi. Mol Biochem Parasitol 77(2):115\u2013125","journal-title":"Mol Biochem Parasitol"},{"issue":"12","key":"9808_CR11","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1111\/j.1462-5822.2006.00755.x","volume":"8","author":"SS Rubin-de-Celis","year":"2006","unstructured":"Rubin-de-Celis SS, Uemura H, Yoshida N, Schenkman S (2006) Expression of trypomastigote trans-sialidase in metacyclic forms of Trypanosoma cruzi increases parasite escape from its parasitophorous vacuole. Cell Microbiol 8(12):1888\u20131898","journal-title":"Cell Microbiol"},{"issue":"5","key":"9808_CR12","first-page":"826","volume":"10","author":"VL Perreira-Chioccola","year":"2003","unstructured":"Perreira-Chioccola VL, Fragata-Filho AA, Levy AM, Rodrigues MM, Schenkman S (2003) Enzyme-linked immunoassay using recombinant trans-sialidase of Trypanosoma cruzi can be employed for monitoring of patients with Chagas\u2019 disease after drug treatment. Clin Diagn Lab Immunol 10(5):826\u2013830","journal-title":"Clin Diagn Lab Immunol"},{"issue":"1","key":"9808_CR13","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1046\/j.1365-3024.2000.00260.x","volume":"22","author":"M Ribeir\u00e3o","year":"2000","unstructured":"Ribeir\u00e3o M, Pereira-Chioccola VL, R\u00e9nia L, Filho AF, Schenkman S, Rodrigues MM (2000) Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase. Parasite Immunol 22(1):49\u201353","journal-title":"Parasite Immunol"},{"issue":"8","key":"9808_CR14","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1016\/S0264-410X(97)00277-6","volume":"16","author":"F Costa","year":"1998","unstructured":"Costa F, Franchin G, Pereira-Chioccola VL, Ribeir\u00e3o M, Schenkman S, Rodrigues MM (1998) Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice. Vaccine 16(8):768\u2013774","journal-title":"Vaccine"},{"issue":"15","key":"9808_CR15","doi-asserted-by":"publisher","first-page":"2246","DOI":"10.1002\/cbic.201100421","volume":"12","author":"R Schauer","year":"2011","unstructured":"Schauer R, Kamerling JP (2011) The chemistry and biology of trypanosomal trans-sialidases: virulence factor in Chagas disease and sleeping sickness. Chembiochem 12(15):2246\u20132264","journal-title":"Chembiochem"},{"issue":"2","key":"9808_CR16","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/0001-706X(95)00077-R","volume":"59","author":"M Engstler","year":"1995","unstructured":"Engstler M, Schauer R, Brun R (1995) Distribution of developmentally regulated trans-sialidases in the Kinetoplastida and characterization of a shed trans-sialidase activity from procyclic Trypanosoma congolense. Acta Trop 59(2):117\u2013129","journal-title":"Acta Trop"},{"issue":"8","key":"9808_CR17","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1515\/BC.2003.133","volume":"384","author":"E Tiralongo","year":"2003","unstructured":"Tiralongo E, Martensen I, Gr\u00f6tzinger J, Tiralongo J, Schauer R (2003) Trans-sialidase-like sequences from Trypanosoma congolense conserve most of the critical active site residues found in other trans-sialidases. Biol Chem 384(8):1203\u20131213","journal-title":"Biol Chem"},{"issue":"26","key":"9808_CR18","doi-asserted-by":"publisher","first-page":"23301","DOI":"10.1074\/jbc.M212909200","volume":"278","author":"E Tiralongo","year":"2003","unstructured":"Tiralongo E, Schrader S, Lange H, Lemke H, Tiralongo J, Schauer R (2003) Two trans-sialidase forms with different sialic acid transfer and sialidase activities from Trypanosoma congolense. J Biol Chem 278(26):23301\u201323310","journal-title":"J Biol Chem"},{"issue":"10","key":"9808_CR19","doi-asserted-by":"publisher","first-page":"e1003710","DOI":"10.1371\/journal.ppat.1003710","volume":"9","author":"Z Ammar","year":"2013","unstructured":"Ammar Z, Plazolles N, Baltz T, Coustou V (2013) Identification of trans-sialidases as a common mediator of endothelial cell activation by African trypanosomes. PloS Pathog 9(10):e1003710. https:\/\/doi.org\/10.1371\/journal.ppat.1003710","journal-title":"PloS Pathog"},{"issue":"5","key":"9808_CR20","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1007\/s00436-009-1542-6","volume":"105","author":"MS Silva","year":"2009","unstructured":"Silva MS, Prazeres DM, Lan\u00e7a A, Atouguia J, Monteiro GA (2009) Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis. Parasitol Res 105(5):1223\u20131229","journal-title":"Parasitol Res"},{"key":"9808_CR21","volume-title":"Report of a WHO informal consultation on sustainable control of human African trypanosomiasis","author":"World Health Organization","year":"2007","unstructured":"World Health Organization (2007) Report of a WHO informal consultation on sustainable control of human African trypanosomiasis. Geneva, Switzerland"},{"issue":"14","key":"9808_CR22","doi-asserted-by":"publisher","first-page":"1862","DOI":"10.1017\/S0031182016001268","volume":"143","author":"F Giordani","year":"2016","unstructured":"Giordani F, Morrison LJ, Rowan TG, Koning DE, Barrett HP, M. P (2016) The animal trypanosomiasis and their chemotherapy: a review. Parasitology 143(14):1862\u20131889","journal-title":"Parasitology"},{"issue":"10","key":"9808_CR23","doi-asserted-by":"publisher","first-page":"718","DOI":"10.2471\/BLT.13.126474","volume":"91","author":"V Lejon","year":"2013","unstructured":"Lejon V, Jacobs J, Simarro PP (2013) Elimination of sleeping sickness hindered by difficult diagnosis. Bull World Health Organ 91(10):718","journal-title":"Bull World Health Organ"},{"issue":"2","key":"9808_CR24","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.bbrc.2011.10.085","volume":"415","author":"F Nakatani","year":"2011","unstructured":"Nakatani F, Morita YS, Ashida H, Nagamune K, Maeda Y, Kinoshita T (2011) Identification of a second catalytically active trans-sialidase in Trypanosoma brucei. Biochem Biophys Res Commun 415(2):421\u2013425","journal-title":"Biochem Biophys Res Commun"},{"issue":"1","key":"9808_CR25","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1128\/IAI.73.1.201-207.2005","volume":"74","author":"MV Tribulatti","year":"2005","unstructured":"Tribulatti MV, Mucci J, Van Rooijen N, Leguizam\u00f3n MS, Campetella O (2005) The trans-sialidase from Trypanosoma cruzi induces thrombocytopenia during acute Chagas\u2019disease by reducing the platelet sialic acid contents. Infect Immun 74(1):201\u2013207","journal-title":"Infect Immun"},{"issue":"10","key":"9808_CR26","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1084\/jem.20030635","volume":"199","author":"K Nagamune","year":"2004","unstructured":"Nagamune K, Acosta-Serrano A, Uemura H, Brun R, Kunz-Renggli C, Maeda Y, Ferguson MA, Kinoshita T (2004) Surface sialic acids taken from the host allow trypanosome survival in tsetse fly vectors. J Exp Med 199(10):1445\u20131450","journal-title":"J Exp Med"},{"key":"9808_CR27","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/0001-706X(93)90093-Q","volume":"54","author":"FW Jennings","year":"1993","unstructured":"Jennings FW (1993) Combination chemotherapy of CNS trypanosomiasis. Acta Trop 54:205\u2013213","journal-title":"Acta Trop"},{"issue":"1","key":"9808_CR28","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.exppara.2010.06.017","volume":"127","author":"AS Lan\u00e7a","year":"2011","unstructured":"Lan\u00e7a AS, de Sousa KP, Atouguia J, Prazeres DM, Monteiro GA, Silva MS (2011) Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis. Exp Parasitol 127(1):18\u201324","journal-title":"Exp Parasitol"},{"issue":"4","key":"9808_CR29","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1007\/s10930-010-9250-8","volume":"29","author":"KP de Sousa","year":"2010","unstructured":"de Sousa KP, Atouguia J, Silva MS (2010) Partial biochemical characterization of a metalloproteinase from the bloodstream forms of Trypanosoma brucei brucei parasites. Protein J 29(4):283\u2013289","journal-title":"Protein J"},{"key":"9808_CR30","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1006\/abio.1998.2977","volume":"268","author":"CA Retamal","year":"1999","unstructured":"Retamal CA, Thiebaut P, Alves EW (1999) Protein purification from polyacrylamide gels by sonication extraction. Anal Biochem 268:15\u201320","journal-title":"Anal Biochem"},{"issue":"3","key":"9808_CR31","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/0014-4894(70)90120-7","volume":"28","author":"SM Lanham","year":"1970","unstructured":"Lanham SM, Godfrey DG (1970) Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp Parasitol 28(3):521\u2013534","journal-title":"Exp Parasitol"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-018-09808-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-018-09808-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-018-09808-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T05:22:03Z","timestamp":1744176123000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-018-09808-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,2]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2,15]]}},"alternative-id":["9808"],"URL":"https:\/\/doi.org\/10.1007\/s10930-018-09808-1","relation":{},"ISSN":["1572-3887","1875-8355"],"issn-type":[{"type":"print","value":"1572-3887"},{"type":"electronic","value":"1875-8355"}],"subject":[],"published":{"date-parts":[[2019,1,2]]},"assertion":[{"value":"2 January 2019","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Murine model was performed in accordance with existing legislation in Portugal and in accordance of the Ethical Committee at Institute of Hygiene and Tropical Medicine, Lisbon\u2014Portugal.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}}]}}