{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T12:00:50Z","timestamp":1740139250319,"version":"3.37.3"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T00:00:00Z","timestamp":1518393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T00:00:00Z","timestamp":1518393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"University of P\u00e9cs, Medical School Research foundation, PTE KA","award":["2015\/30019\/KA"],"award-info":[{"award-number":["2015\/30019\/KA"]}]},{"name":"European Union, European Social Fund","award":["EFOP-3.6.1-16-2016-00004"],"award-info":[{"award-number":["EFOP-3.6.1-16-2016-00004"]}]},{"name":"University of P\u00e9cs, Pharmaceutic Talent Center program","award":["PST 480132"],"award-info":[{"award-number":["PST 480132"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1007\/s10930-018-9759-9","type":"journal-article","created":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T01:53:57Z","timestamp":1518400437000},"page":"113-121","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The C19S Substitution Enhances the Stability of Hepcidin While Conserving Its Biological Activity"],"prefix":"10.1007","volume":"37","author":[{"given":"Edina","family":"Pandur","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsanna","family":"Fekete","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kitti","family":"Tam\u00e1si","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"Grama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edit","family":"Varga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6706-2682","authenticated-orcid":false,"given":"Katalin","family":"Sipos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,2,12]]},"reference":[{"key":"9759_CR1","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/S0014-5793(00)01920-7","volume":"480","author":"A Krause","year":"2000","unstructured":"Krause A, Neitz S, Schulz A et al (2000) LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity. FEBS Lett 480:147\u2013150","journal-title":"FEBS Lett"},{"key":"9759_CR2","doi-asserted-by":"publisher","first-page":"7806","DOI":"10.1074\/jbc.M008922200","volume":"276","author":"CH Park","year":"2001","unstructured":"Park CH, Valore EV, Waring AJ, Ganz T (2001) Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J Biol Chem 276:7806\u20137810","journal-title":"J Biol Chem"},{"key":"9759_CR3","doi-asserted-by":"publisher","first-page":"7811","DOI":"10.1074\/jbc.M008923200","volume":"276","author":"C Pigeon","year":"2001","unstructured":"Pigeon C, Ilyin G, Courselaud B et al (2001) A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload. J Biol Chem 276:7811\u20137819","journal-title":"J Biol Chem"},{"key":"9759_CR4","doi-asserted-by":"publisher","first-page":"8780","DOI":"10.1073\/pnas.151179498","volume":"98","author":"G Nicolas","year":"2001","unstructured":"Nicolas G, Bennoun M, Devaux I et al (2001) Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice. Proc Natl Acad Sci USA 98:8780\u20138785","journal-title":"Proc Natl Acad Sci USA"},{"key":"9759_CR5","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1373\/clinchem.2009.140053","volume":"57","author":"JJC Kroot","year":"2011","unstructured":"Kroot JJC, Tjalsma H, Fleming RE, Swinkels DW (2011) Hepcidin in human iron disorders: diagnostic implications. Clin Chem 57:1650\u20131669","journal-title":"Clin Chem"},{"key":"9759_CR6","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.1136\/gut.2007.141812","volume":"57","author":"N Scamuffa","year":"2008","unstructured":"Scamuffa N, Basak A, Lalou C et al (2008) Regulation of prohepcidin processing and activity by the subtilisin-like proprotein convertases Furin, PC5, PACE4 and PC7. Gut 57:1573\u20131582","journal-title":"Gut"},{"key":"9759_CR7","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.bcmd.2007.07.009","volume":"40","author":"EV Valore","year":"2008","unstructured":"Valore EV, Ganz T (2008) Posttranslational processing of hepcidin in human hepatocytes is mediated by the prohormone convertase furin. Blood Cells Mol Dis 40:132\u2013138","journal-title":"Blood Cells Mol Dis"},{"key":"9759_CR8","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.bcmd.2009.03.008","volume":"43","author":"M Schranz","year":"2009","unstructured":"Schranz M, Bakry R, Creus M et al (2009) Activation and inactivation of the iron hormone hepcidin: Biochemical characterization of prohepcidin cleavage and sequential degradation to N-terminally truncated hepcidin isoforms. Blood Cells Mol Dis 43:169\u2013179","journal-title":"Blood Cells Mol Dis"},{"key":"9759_CR9","doi-asserted-by":"publisher","first-page":"24155","DOI":"10.1074\/jbc.M109.017764","volume":"284","author":"JB Jordan","year":"2009","unstructured":"Jordan JB, Poppe L, Haniu M et al (2009) Hepcidin revisited, disulfide connectivity, dynamics, and structure. J Biol Chem 284:24155\u201324167","journal-title":"J Biol Chem"},{"key":"9759_CR10","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.cmet.2005.01.003","volume":"1","author":"A Donovan","year":"2005","unstructured":"Donovan A, Lima CA, Pinkus JL et al (2005) The iron exporter ferroportin\/Slc40a1 is essential for iron homeostasis. Cell Metab 1:191\u2013200","journal-title":"Cell Metab"},{"key":"9759_CR11","doi-asserted-by":"publisher","first-page":"2090","DOI":"10.1126\/science.1104742","volume":"306","author":"E Nemeth","year":"2008","unstructured":"Nemeth E (2008) Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 306:2090\u20132093","journal-title":"Science"},{"key":"9759_CR12","doi-asserted-by":"publisher","first-page":"2569","DOI":"10.1091\/mbc.E07-01-0060","volume":"18","author":"I De Domenico","year":"2007","unstructured":"De Domenico I, McVey Ward D, Langelier C et al (2007) The molecular mechanism of hepcidin-mediated ferroportin down-regulation. Mol Biol Cell 18:2569\u20132578","journal-title":"Mol Biol Cell"},{"key":"9759_CR13","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1182\/blood-2005-05-2049","volume":"107","author":"E Nemeth","year":"2006","unstructured":"Nemeth E, Preza GC, Jung CL et al (2006) The N-terminus of hepcidin is essential for its interaction with ferroportin: structure-function study. Blood 107:328\u2013333","journal-title":"Blood"},{"key":"9759_CR14","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1136\/gut.2007.131722","volume":"57","author":"T Chaston","year":"2008","unstructured":"Chaston T, Chung B, Mascarenhas M et al (2008) Evidence for differential effects of hepcidin in macrophages and intestinal epithelial cells. Gut 57:374\u2013382","journal-title":"Gut"},{"key":"9759_CR15","first-page":"G192-G198","volume":"294","author":"NP Mena","year":"2007","unstructured":"Mena NP, Esparza A, Tapia V et al (2007) Hepcidin inhibits apical iron uptake in intestinal cells. Am J Physiol Gastrointest Liver Physiol 294:G192-G198","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"9759_CR16","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1053\/j.gastro.2010.12.037","volume":"140","author":"C Brasse-Lagnel","year":"2011","unstructured":"Brasse-Lagnel C, Karim Z, Letterton P et al (2011) Intestinal DMT1 contransporter is down-regulated by hepcidin via proteasome internalization and degradation. Gastroenterology 140:1261\u20131271","journal-title":"Gastroenterology"},{"key":"9759_CR17","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.chembiol.2010.12.009","volume":"18","author":"RJ Clark","year":"2011","unstructured":"Clark RJ, Tan CC, Preza GC et al (2011) Understanding the structure\/activity relationships of the iron regulatory peptide hepcidin. Chem Biol 18:336\u2013343","journal-title":"Chem Biol"},{"key":"9759_CR18","doi-asserted-by":"publisher","first-page":"4880","DOI":"10.1172\/JCI57693","volume":"121","author":"GC Preza","year":"2011","unstructured":"Preza GC, Ruchala P, Pinon R et al (2011) Minihepcidins are rationally designed small peptides that mimic hepcidin activity in mice and may be useful for the treatment of iron overload. J Clin Invest 121:4880\u20134888","journal-title":"J Clin Invest"},{"key":"9759_CR19","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1182\/blood-2008-03-146134","volume":"114","author":"A Fernandes","year":"2009","unstructured":"Fernandes A, Preza GC, Phung Y et al (2009) The molecular basis of hepcidin-resistant hereditary hemochromatosis. Blood 114:437\u2013443","journal-title":"Blood"},{"key":"9759_CR20","doi-asserted-by":"publisher","first-page":"2012","DOI":"10.1111\/j.1742-4658.2009.06937.x","volume":"276","author":"E Pandur","year":"2008","unstructured":"Pandur E, Nagy J, Po\u00f3r VS, Sarnyai A et al (2008) Alpha-1 antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum. FEBS J 276:2012\u20132021","journal-title":"FEBS J"},{"key":"9759_CR21","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1111\/ijlh.12358","volume":"37","author":"J Arezes","year":"2015","unstructured":"Arezes J, Nemeth E (2015) Hepcidin and iron disorders: new biology and clinical approaches. Int J Lab Hematol 37:92\u201398","journal-title":"Int J Lab Hematol"},{"key":"9759_CR22","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/j.ab.2004.03.049","volume":"331","author":"J Riemer","year":"2004","unstructured":"Riemer J, Hoepken HH, Czerwinska H et al (2004) Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells. Anal Biochem 331:370\u2013375","journal-title":"Anal Biochem"},{"key":"9759_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphar.2016.00160","volume":"7","author":"G Sebastiani","year":"2016","unstructured":"Sebastiani G, Wilkinson N, Pantopoulos K (2016) Pharmacological targeting of the hepcidin\/ferroportin axis. Front Pharmacol 7:1\u201311","journal-title":"Front Pharmacol"},{"key":"9759_CR24","doi-asserted-by":"publisher","first-page":"1721","DOI":"10.1152\/physrev.00008.2013","volume":"93","author":"T Ganz","year":"2013","unstructured":"Ganz T (2013) Systemic iron homeostasis. Physiol Rev 93:1721\u20131741","journal-title":"Physiol Rev"},{"key":"9759_CR25","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1016\/S0140-6736(15)01315-X","volume":"388","author":"LW Powell","year":"2016","unstructured":"Powell LW, Seckington RC, Deugnier Y(2016) Haemochromatosis Lancet 388:706\u2013716","journal-title":"Haemochromatosis Lancet"},{"key":"9759_CR26","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1053\/j.gastro.2010.06.013","volume":"139","author":"A Pietrangelo","year":"2010","unstructured":"Pietrangelo A (2010) Hereditary hemochromatosis: pathogenesis, diagnosis, and treatment. Gastroenterology 139:393\u2013408","journal-title":"Gastroenterology"},{"key":"9759_CR27","doi-asserted-by":"publisher","first-page":"3829","DOI":"10.1182\/blood-2012-07-440743","volume":"120","author":"E Nemeth","year":"2012","unstructured":"Nemeth E, Ramos E, Ruchala P et al (2012) Minihepcidins prevent iron overload in a hepcidin-deficient mouse model of severe hemochromatosis. Blood 120:3829\u20133836","journal-title":"Blood"},{"key":"9759_CR28","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1182\/blood-2015-10-676742","volume":"128","author":"C Casu","year":"2016","unstructured":"Casu C, Oikonomidou PR, Chen H et al (2016) Minihepcidin peptides as disease modifiers in mice affected by \u03b2-thalassemia and polycythemia vera. Blood 128:265\u2013276","journal-title":"Blood"},{"key":"9759_CR29","doi-asserted-by":"publisher","first-page":"4961","DOI":"10.1016\/j.bmcl.2015.03.012","volume":"25","author":"K Chua","year":"2015","unstructured":"Chua K, Fung E, Micewicz ED et al (2015) Small cyclic agonists of iron regulatory hormone hepcidin. Bioorg Med Chem Lett 25:4961\u20134969","journal-title":"Bioorg Med Chem Lett"},{"key":"9759_CR30","doi-asserted-by":"publisher","first-page":"2407","DOI":"10.1182\/blood-2003-10-3390","volume":"103","author":"A Roetto","year":"2004","unstructured":"Roetto A, Daraio F, Porporato P et al (2004) Screening hepcidin for mutations in juvenile hemochromatosis: identification of a new mutation (C70R). Blood 103:2407\u20132409","journal-title":"Blood"},{"key":"9759_CR31","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1111\/j.0009-9163.2004.00254.x","volume":"65","author":"MB Delatycki","year":"2004","unstructured":"Delatycki MB, Allen KJ, Gow P et al (2004) A homozygous HAMP mutation in a multiply consanguineous family with pseudo-dominant juvenile hemochromatosis. Clin Genet 65:378\u2013383","journal-title":"Clin Genet"},{"key":"9759_CR32","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1007\/s00431-002-1114-6","volume":"162","author":"M De Gobbi","year":"2003","unstructured":"De Gobbi M, Caruso R, Daraio F et al (2003) Diagnosis of juvenile hemochromatosis in an 11-year-old child combining genetic analysis and non-invasive liver iron quantitation. Eur J Pediatr 162:96\u201399","journal-title":"Eur J Pediatr"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-018-9759-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-018-9759-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-018-9759-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:20:13Z","timestamp":1667521213000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-018-9759-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,12]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["9759"],"URL":"https:\/\/doi.org\/10.1007\/s10930-018-9759-9","relation":{},"ISSN":["1572-3887","1875-8355"],"issn-type":[{"type":"print","value":"1572-3887"},{"type":"electronic","value":"1875-8355"}],"subject":[],"published":{"date-parts":[[2018,2,12]]},"assertion":[{"value":"12 February 2018","order":1,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}