{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:39:48Z","timestamp":1792622388021,"version":"4.5.0"},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100005117","name":"Birjand University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005117","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1007\/s10930-021-10022-9","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T10:14:06Z","timestamp":1632910446000},"page":"907-916","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Recombinant Production of Bovine Enteropeptidase Light Chain in SHuffle\u00ae T7 Express and Optimization of Induction Parameters"],"prefix":"10.1007","volume":"40","author":[{"given":"Mohammad","family":"Shoae","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Safarpour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1863-8294","authenticated-orcid":false,"given":"Mohsen","family":"Khorashadizadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,29]]},"reference":[{"issue":"3","key":"10022_CR1","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1080\/10826068.2014.907185","volume":"45","author":"M Azhar","year":"2015","unstructured":"Azhar M, Somashekhar R (2015) Production and purification of recombinant enteropeptidase expressed in an insect\u2013baculovirus cell system. Prep Biochem Biotechnol 45(3):268\u2013278","journal-title":"Prep Biochem Biotechnol"},{"issue":"6","key":"10022_CR2","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.1007\/s10529-011-0562-3","volume":"33","author":"H Chun","year":"2011","unstructured":"Chun H, Joo K, Lee J, Shin H-C (2011) Design and efficient production of bovine enterokinase light chain with higher specificity in E. coli. Biotechnol Lett 33(6):1227\u20131232","journal-title":"Biotechnol Lett"},{"issue":"1","key":"10022_CR3","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S1046-5928(03)00159-1","volume":"31","author":"ME Gasparian","year":"2003","unstructured":"Gasparian ME, Ostapchenko VG, Schulga AA, Dolgikh DA, Kirpichnikov MP (2003) Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli. Protein Expr Purif 31(1):133\u2013139","journal-title":"Protein Expr Purif"},{"issue":"5","key":"10022_CR4","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1007\/s00253-016-7960-3","volume":"101","author":"K Melicherov\u00e1","year":"2017","unstructured":"Melicherov\u00e1 K, Krahulec J, \u0160afr\u00e1nek M, Li\u0161kov\u00e1 V, Hopkov\u00e1 D, Sz\u00e9liov\u00e1 D, Tur\u0148a J (2017) \u00a0Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris. Appl Microbiol Biotechnol 101(5):1927\u20131934","journal-title":"Appl Microbiol Biotechnol"},{"key":"10022_CR5","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.ijbiomac.2017.02.052","volume":"99","author":"H Safarpour","year":"2017","unstructured":"Safarpour H, Banadkoki SB, Keshavarzi Z, Morowvat MH, Soleimanpour M, Pourmolaei S, Shirazi FH (2017) Expression analysis and ATR-FTIR characterization of the secondary structure of recombinant human TNF-\u03b1 from Escherichia coli SHuffle\u00ae T7 Express and BL21 (DE3) cells. Int J Biol Macromol 99:173\u2013178","journal-title":"Int J Biol Macromol"},{"issue":"1","key":"10022_CR6","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1002\/cpps.11","volume":"85","author":"G Ren","year":"2016","unstructured":"Ren G, Ke N, Berkmen M (2016) Use of the SHuffle strains in production of proteins. Curr Protoc Protein Sci 85(1):5\u201326","journal-title":"Curr Protoc Protein Sci"},{"issue":"Special Issue","key":"10022_CR7","first-page":"157","volume":"18","author":"P Panahi Chegini","year":"2019","unstructured":"Panahi Chegini P, Nikokar I, Tabarzad M, Faezi S, Mahboubi A (2019) Effect of amino acid substitutions on biological activity of antimicrobial peptide: design, recombinant production, and biological activity. Iran J Pharm Res 18(Special Issue):157\u2013168","journal-title":"Iran J Pharm Res"},{"key":"10022_CR8","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.jpba.2018.05.005","volume":"157","author":"S Tengattini","year":"2018","unstructured":"Tengattini S, Rinaldi F, Piubelli L, Kupfer T, Peters B, Bavaro T, Calleri E, Massolini G, Temporini C (2018) Enterokinase monolithic bioreactor as an efficient tool for biopharmaceuticals preparation: on-line cleavage of fusion proteins and analytical characterization of released products. J Pharm Biomed Anal 157:10\u201319","journal-title":"J Pharm Biomed Anal"},{"issue":"2","key":"10022_CR9","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1006\/jmbi.1999.3089","volume":"292","author":"D Lu","year":"1999","unstructured":"Lu D, F\u00fctterer K, Korolev S, Zheng X, Tan K, Waksman G, Sadler JE (1999) Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide. J Mol Biol 292(2):361\u2013373","journal-title":"J Mol Biol"},{"issue":"3","key":"10022_CR10","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1080\/10826060701386695","volume":"37","author":"L Huang","year":"2007","unstructured":"Huang L, Ruan H, Gu W, Xu Z, Cen P, Fan L (2007) Functional expression and purification of bovine enterokinase light chain in recombinant Escherichia coli. Prep Biochem Biotechnol 37(3):205\u2013217","journal-title":"Prep Biochem Biotechnol"},{"issue":"4","key":"10022_CR11","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.jbiotec.2013.10.022","volume":"168","author":"W Skala","year":"2013","unstructured":"Skala W, Goettig P, Brandstetter H (2013) Do-it-yourself histidine-tagged bovine enterokinase: a handy member of the protein engineer\u2019s toolbox. J Biotechnol 168(4):421\u2013425","journal-title":"J Biotechnol"},{"issue":"2","key":"10022_CR12","doi-asserted-by":"publisher","first-page":"416","DOI":"10.3390\/ijms21020416","volume":"21","author":"ADLC Pech-Canul","year":"2020","unstructured":"Pech-Canul ADLC, Carrillo-Campos J, Ballinas-Casarrubias ML, Solis-Oviedo RL, Hern\u00e1ndez-Rasc\u00f3n SK, Hern\u00e1ndez-Ochoa LR, Guti\u00e9rrez-M\u00e9ndez N, Garc\u00eda-Triana A (2020) Functional expression and one-step protein purification of manganese peroxidase 1 (rMnP1) from Phanerochaete chrysosporium using the E. coli-expression system. Int J Mol Sci 21(2):416","journal-title":"Int J Mol Sci"},{"key":"10022_CR13","doi-asserted-by":"publisher","first-page":"35430","DOI":"10.1038\/srep35430","volume":"6","author":"W Xu","year":"2016","unstructured":"Xu W, Zhao Q, Xing L, Lin Z (2016) Recombinant production of influenza hemagglutinin and HIV-1 GP120 antigenic peptides using a cleavable self-aggregating tag. Sci Rep 6:35430","journal-title":"Sci Rep"},{"key":"10022_CR14","first-page":"1","volume":"23","author":"A Talaei","year":"2021","unstructured":"Talaei A, Mazaheri S, Bayat E, Bakhshandeh B, Sabzalinejad M, Damough S, Mahboudi F, Nematollahi L, Talebkhan Y (2021) Production of soluble and functional Anti-TNF-\u03b1 Fab\u2019 fragment in cytoplasm of E. coli: investigating the effect of process conditions on cellular biomass and protein yield using response surface methodology. Protein J 23:1\u20133","journal-title":"Protein J"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-021-10022-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-021-10022-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-021-10022-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:34:32Z","timestamp":1667522072000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-021-10022-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,29]]},"references-count":14,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10022"],"URL":"https:\/\/doi.org\/10.1007\/s10930-021-10022-9","relation":{},"ISSN":["1572-3887","1875-8355"],"issn-type":[{"value":"1572-3887","type":"print"},{"value":"1875-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,29]]},"assertion":[{"value":"20 September 2021","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2021","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that there is no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Publish"}}]}}