{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:36:04Z","timestamp":1789673764889,"version":"4.0.1"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100003968","name":"Iran National Science Foundation","doi-asserted-by":"publisher","award":["99014455"],"award-info":[{"award-number":["99014455"]}],"id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003968","name":"Iran National Science Foundation","doi-asserted-by":"publisher","award":["99014455"],"award-info":[{"award-number":["99014455"]}],"id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012155","name":"National Institute for Medical Research Development","doi-asserted-by":"publisher","award":["964854"],"award-info":[{"award-number":["964854"]}],"id":[{"id":"10.13039\/501100012155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012155","name":"National Institute for Medical Research Development","doi-asserted-by":"publisher","award":["964854"],"award-info":[{"award-number":["964854"]}],"id":[{"id":"10.13039\/501100012155","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"published-print":{"date-parts":[[2024,2]]},"DOI":"10.1007\/s10930-023-10164-y","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T13:02:10Z","timestamp":1701176530000},"page":"24-38","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Efficient Expression in the Prokaryotic Host System, Purification and Structural Analyses of the Recombinant Human ACE2 Catalytic Subunit as a Hybrid Protein with the B Subunit of Cholera Toxin (CTB-ACE2)"],"prefix":"10.1007","volume":"43","author":[{"given":"Maryam","family":"Ghahramani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Bagher","family":"Shahsavani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Hossein","family":"Khaleghinejad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Niazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Akbar","family":"Moosavi-Movahedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Yousefi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"10164_CR1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.18553\/jmcp.2007.13.s8-b.9","volume":"13","author":"SA Atlas","year":"2007","unstructured":"Atlas SA (2007) The renin-angiotensin aldosterone system: pathophysiological role and pharmacologic inhibition. J Manag Care Pharm JMCP 13:9\u201320. https:\/\/doi.org\/10.18553\/jmcp.2007.13.s8-b.9","journal-title":"J Manag Care Pharm JMCP"},{"key":"10164_CR2","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.omtm.2019.06.007","volume":"14","author":"A Verma","year":"2019","unstructured":"Verma A, Xu K, Du T, Zhu P, Liang Z, Liao S, Zhang J, Raizada MK, Grant MB, Li Q (2019) Expression of human ACE2 in Lactobacillus and Beneficial effects in diabetic retinopathy in mice. Mol Ther Methods Clin Dev 14:161\u2013170. https:\/\/doi.org\/10.1016\/j.omtm.2019.06.007","journal-title":"Mol Ther Methods Clin Dev"},{"key":"10164_CR3","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1002\/path.1570","volume":"203","author":"I Hamming","year":"2004","unstructured":"Hamming I, Timens W, Bulthuis MLC, Lely AT, Navis GJ, van Goor H (2004) Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol 203:631\u2013637. https:\/\/doi.org\/10.1002\/path.1570","journal-title":"J Pathol"},{"issue":"2020","key":"10164_CR4","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1515\/cclm-2020-0727","volume":"58","author":"G Lippi","year":"2020","unstructured":"Lippi G, Lavie CJ, Henry BM, Sanchis-Gomar F (2020) Do genetic polymorphisms in angiotensin converting enzyme 2 (ACE2) gene play a role in coronavirus disease 2019 (COVID-19)? Clin Chem Lab Med 58(2020):1415\u20131422. https:\/\/doi.org\/10.1515\/cclm-2020-0727","journal-title":"Clin Chem Lab Med"},{"key":"10164_CR5","doi-asserted-by":"publisher","first-page":"30113","DOI":"10.1074\/jbc.M505111200","volume":"280","author":"DW Lambert","year":"2005","unstructured":"Lambert DW, Yarski M, Warner FJ, Thornhill P, Parkin ET, Smith AI, Hooper NM, Turner AJ (2005) Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2). J Biol Chem 280:30113\u201330119. https:\/\/doi.org\/10.1074\/jbc.M505111200","journal-title":"J Biol Chem"},{"key":"10164_CR6","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.ijbiomac.2011.03.018","volume":"49","author":"DS Arag\u00e3o","year":"2011","unstructured":"Arag\u00e3o DS, Cunha TS, Arita DY, Andrade MCC, Fernandes AB, Watanabe IKM, Mortara RA, Casarini DE (2011) Purification and characterization of angiotensin converting enzyme 2 (ACE2) from murine model of mesangial cell in culture. Int J Biol Macromol 49:79\u201384. https:\/\/doi.org\/10.1016\/j.ijbiomac.2011.03.018","journal-title":"Int J Biol Macromol"},{"key":"10164_CR7","doi-asserted-by":"publisher","first-page":"33238","DOI":"10.1074\/jbc.M002615200","volume":"275","author":"SR Tipnis","year":"2000","unstructured":"Tipnis SR, Hooper NM, Hyde R, Karran E, Christie G, Turner AJ (2000) A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol Chem 275:33238\u201333243. https:\/\/doi.org\/10.1074\/jbc.M002615200","journal-title":"J Biol Chem"},{"key":"10164_CR8","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1038\/nature00786","volume":"417","author":"MA Crackower","year":"2002","unstructured":"Crackower MA, Sarao R, Oudit GY, Yagil C, Kozieradzki I, Scanga SE, Oliveira-dos-Santos AJ, da Costa J, Zhang L, Pei Y, Scholey J, Ferrario CM, Manoukian AS, Chappell MC, Backx PH, Yagil Y, Penninger JM (2002) Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature 417:822\u2013828. https:\/\/doi.org\/10.1038\/nature00786","journal-title":"Nature"},{"key":"10164_CR9","doi-asserted-by":"publisher","DOI":"10.1101\/2020.03.12.988634","author":"H Xiao","year":"2020","unstructured":"Xiao H, Nie X-T, Ji X-X, Yan S, Zhu B, Zhang Y-S (2020) Establishing prokaryotic expression system of angiotensin-converting enzyme 2 (ACE2) gene in pigs. BioRxiv. https:\/\/doi.org\/10.1101\/2020.03.12.988634","journal-title":"BioRxiv"},{"key":"10164_CR10","doi-asserted-by":"publisher","DOI":"10.1002\/bip.23350","volume":"111","author":"M Ghahramani","year":"2020","unstructured":"Ghahramani M, Yousefi R, Niazi A, Kurganov B (2020) The congenital cataract-causing mutations P20R and A171T are associated with important changes in the amyloidogenic feature, structure and chaperone-like activity of human \u03b1B-crystallin. Biopolymers 111:e23350","journal-title":"Biopolymers"},{"key":"10164_CR11","doi-asserted-by":"publisher","DOI":"10.1002\/bip.23350","volume":"111","author":"M Ghahramani","year":"2020","unstructured":"Teale FWJ, Weber G (1957) Ultraviolet fluorescence of the aromatic amino acids. Biochem\nJ 65:476","journal-title":"Biopolymers"},{"key":"10164_CR12","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1007\/s10895-007-0247-4","volume":"18","author":"Y-Z Zhang","year":"2008","unstructured":"Zhang Y-Z, Zhou B, Liu Y-X, Zhou C-X, Ding X-L, Liu Y (2008) Fluorescence study on the interaction of bovine serum albumin with p-aminoazobenzene. J Fluoresc 18:109\u2013118","journal-title":"J Fluoresc"},{"key":"10164_CR13","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1016\/j.bbapap.2017.02.003","volume":"1865","author":"K Khoshaman","year":"2017","unstructured":"Khoshaman K, Oryan A, Moosavi-Movahedi AA, Tamaddon AM, Kurganov BI, Yousefi R, Abolmaali SS (2017) impact of different mutations at Arg54 on structure, chaperone-like activity and oligomerization state of human \u00ce\u00b1A-crystallin: The pathomechanism underlying congenital cataract-causing mutations R54L, R54P and R54C. BBA-Proteins Proteom 1865:604\u2013618","journal-title":"BBA-Proteins Proteom"},{"key":"10164_CR14","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/j.jfda.2014.03.004","volume":"22","author":"E Maltas","year":"2014","unstructured":"Maltas E (2014) Binding interactions of niclosamide with serum proteins. J Food Drug Anal 22:549\u2013555","journal-title":"J Food Drug Anal"},{"key":"10164_CR15","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/j.jfda.2014.03.004","volume":"22","author":"E Maltas","year":"2014","unstructured":"Sharma KK, Kumar GS, Murphy AS, Kester K (1998) Identification of 1, 1\u2032-bi (4-anilino)\nnaphthalene-5, 5\u2032-disulfonic acid binding sequences in \u03b1-crystallin. J Biol Chem 273:15474\u201315478","journal-title":"J Food Drug Anal"},{"key":"10164_CR16","doi-asserted-by":"publisher","first-page":"112870","DOI":"10.1039\/C6RA23426G","volume":"6","author":"A Bortolotti","year":"2016","unstructured":"Bortolotti A, Wong YH, Korsholm SS, Bahring NHB, Bobone S, Tayyab S, van de Weert M, Stella L (2016) On the purported \u201cbackbone fluorescence\u201d in protein three-dimensional fluorescence spectra. Rsc Adv 6:112870\u2013112876","journal-title":"Rsc Adv"},{"key":"10164_CR17","first-page":"3","volume":"51","author":"KR Grigoryan","year":"2017","unstructured":"Grigoryan KR, Shilajyan HA (2017) Fluorescence 2D and 3D spectra analysis of tryptophan, tyrosine and phenylalanine. Chem Biol 51:3\u20137","journal-title":"Chem Biol"},{"key":"10164_CR18","doi-asserted-by":"publisher","first-page":"W668","DOI":"10.1093\/nar\/gkh371","volume":"32","author":"L Whitmore","year":"2004","unstructured":"Whitmore L, Wallace BA (2004) DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data. Nucleic Acids Res 32:W668\u2013W673","journal-title":"Nucleic Acids Res"},{"key":"10164_CR19","doi-asserted-by":"publisher","first-page":"5918","DOI":"10.3390\/app10175918","volume":"10","author":"A Sadat","year":"2020","unstructured":"Sadat A, Joye IJ (2020) Peak fitting applied to Fourier transform infrared and Raman spectroscopic analysis of proteins. Appl Sci 10:5918","journal-title":"Appl Sci"},{"key":"10164_CR20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.124590","volume":"242","author":"SH Khaleghinejad","year":"2023","unstructured":"Khaleghinejad SH, Shahsavani MB, Ghahramani M, Yousefi R (2023) Investigating the role of double mutations R12C\/P20R, and R12C\/R69C on structure, chaperone-like activity, and amyloidogenic properties of human \u03b1B-crystallin. Int J Biol Macromol 242:124590","journal-title":"Int J Biol Macromol"},{"key":"10164_CR21","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.ab.2014.11.016","volume":"472","author":"C Zhou","year":"2015","unstructured":"Zhou C, Qi W, Lewis EN, Carpenter JF (2015) Concomitant Raman spectroscopy and dynamic light scattering for characterization of therapeutic proteins at high concentrations. Anal Biochem 472:7\u201320","journal-title":"Anal Biochem"},{"key":"10164_CR22","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","volume":"166","author":"C Sch\u00e4gger","year":"1987","unstructured":"Sch\u00e4gger H, Von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368\u2013379","journal-title":"Anal Biochem"},{"key":"10164_CR23","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","volume":"166","author":"C Sch\u00e4gger","year":"1987","unstructured":"Gasteiger E, Hoogland C, Gattiker A, et al (2005) Protein identification and analysis tools on the ExPASy server. Springer","journal-title":"Anal Biochem"},{"key":"10164_CR24","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1007\/s11095-007-9516-9","volume":"25","author":"A Hawe","year":"2008","unstructured":"Hawe A, Sutter M, Jiskoot W (2008) Extrinsic fluorescent dyes as tools for protein characterization. Pharm Res 25:1487\u20131499","journal-title":"Pharm Res"},{"key":"10164_CR25","doi-asserted-by":"publisher","first-page":"W202","DOI":"10.1093\/nar\/gkn255","volume":"36","author":"S Montgomerie","year":"2008","unstructured":"Montgomerie S, Cruz JA, Shrivastava S, Arndt D, Berjanskii M, Wishart DS (2008) PROTEUS2: a web server for comprehensive protein structure prediction and structure-based annotation. Nucleic Acids Res 36:W202\u2013W209. https:\/\/doi.org\/10.1093\/nar\/gkn255","journal-title":"Nucleic Acids Res"},{"key":"10164_CR26","doi-asserted-by":"crossref","unstructured":"Bursell S-E, Yu N-T (1990) Fluorescence and Raman spectroscopy of the crystalline lens.\nNoninvasive Diagn Tech Ophthalmol 319\u2013341","DOI":"10.1007\/978-1-4613-8896-8_17"},{"key":"10164_CR27","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1002\/jrs.4335","volume":"44","author":"A Rygula","year":"2013","unstructured":"Rygula A, Majzner K, Marzec KM, Kaczor A, Pilarczyk M, Baranska M (2013) Raman spectroscopy of proteins: a review. J Raman Spectrosc 44:1061\u20131076","journal-title":"J Raman Spectrosc"},{"key":"10164_CR28","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1002\/0471140864.ps1708s33","volume":"33","author":"JM Benevides","year":"2003","unstructured":"Benevides JM, Overman SA, Thomas GJ Jr (2003) Raman spectroscopy of proteins. Curr Protoc Protein Sci 33:17\u201318","journal-title":"Curr Protoc Protein Sci"},{"key":"10164_CR29","doi-asserted-by":"publisher","first-page":"2861","DOI":"10.1002\/jps.20895","volume":"96","author":"Z Wen","year":"2007","unstructured":"Wen Z (2007) Raman spectroscopy of protein pharmaceuticals. J Pharm Sci 96:2861\u20132878","journal-title":"J Pharm Sci"},{"key":"10164_CR30","doi-asserted-by":"publisher","first-page":"2851","DOI":"10.1042\/CS20200899","volume":"134","author":"L Lubbe","year":"2020","unstructured":"Lubbe L, Cozier GE, Oosthuizen D, Acharya KR, Sturrock ED (2020) ACE2 and ACE: structure-based insights into mechanism, regulation and receptor recognition by SARS-CoV. Clin Sci Lond Engl 1979 134:2851\u20132871. https:\/\/doi.org\/10.1042\/CS20200899","journal-title":"Clin Sci Lond Engl 1979"},{"key":"10164_CR31","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1186\/s13054-017-1882-z","volume":"21","author":"H Zhang","year":"2017","unstructured":"Zhang H, Baker A (2017) Recombinant human ACE2: acing out angiotensin II in ARDS therapy. Crit Care Lond Engl 21:305. https:\/\/doi.org\/10.1186\/s13054-017-1882-z","journal-title":"Crit Care Lond Engl"},{"key":"10164_CR32","doi-asserted-by":"publisher","first-page":"529","DOI":"10.2337\/db09-1218","volume":"59","author":"GY Oudit","year":"2010","unstructured":"Oudit GY, Liu GC, Zhong J, Basu R, Chow FL, Zhou J, Loibner H, Janzek E, Schuster M, Penninger JM, Herzenberg AM, Kassiri Z, Scholey JW (2010) Human recombinant ACE2 reduces the progression of diabetic nephropathy. Diabetes 59:529\u2013538. https:\/\/doi.org\/10.2337\/db09-1218","journal-title":"Diabetes"},{"key":"10164_CR33","doi-asserted-by":"publisher","first-page":"256294","DOI":"10.1155\/2012\/256294","volume":"2012","author":"C Tikellis","year":"2012","unstructured":"Tikellis C, Thomas MC (2012) Angiotensin-converting enzyme 2 (ACE2) is a key modulator of the renin angiotensin system in health and disease. Int J Pept 2012:256294. https:\/\/doi.org\/10.1155\/2012\/256294","journal-title":"Int J Pept"},{"key":"10164_CR34","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1038\/nature03712","volume":"436","author":"Y Imai","year":"2005","unstructured":"Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, Yang P, Sarao R, Wada T, Leong-Poi H, Crackower MA, Fukamizu A, Hui C-C, Hein L, Uhlig S, Slutsky AS, Jiang C, Penninger JM (2005) Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature 436:112\u2013116. https:\/\/doi.org\/10.1038\/nature03712","journal-title":"Nature"},{"key":"10164_CR35","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1007\/s00134-020-05985-9","volume":"46","author":"H Zhang","year":"2020","unstructured":"Zhang H, Penninger JM, Li Y, Zhong N, Slutsky AS (2020) Angiotensin-converting enzyme 2 (ACE2) as a SARS-CoV-2 receptor: molecular mechanisms and potential therapeutic target. Intensive Care Med 46:586\u2013590. https:\/\/doi.org\/10.1007\/s00134-020-05985-9","journal-title":"Intensive Care Med"},{"key":"10164_CR36","doi-asserted-by":"publisher","first-page":"108528","DOI":"10.1016\/j.celrep.2020.108528","volume":"33","author":"T Tada","year":"2020","unstructured":"Tada T, Fan C, Chen JS, Kaur R, Stapleford KA, Gristick H, Dcosta BM, Wilen CB, Nimigean CM, Landau NR (2020) An ACE2 microbody containing a single immunoglobulin fc domain is a potent inhibitor of SARS-CoV-2. Cell Rep 33:108528. https:\/\/doi.org\/10.1016\/j.celrep.2020.108528","journal-title":"Cell Rep"},{"key":"10164_CR37","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1186\/s12933-021-01286-7","volume":"20","author":"N D\u2019Onofrio","year":"2021","unstructured":"D\u2019Onofrio N, Scisciola L, Sardu C, Trotta MC, De Feo M, Maiello C, Mascolo P, De Micco F, Turriziani F, Municin\u00f2 E, Monetti P, Lombardi A, Napolitano MG, Marino FZ, Ronchi A, Grimaldi V, Hermenean A, Rizzo MR, Barbieri M, Franco R, Pietro Campobasso C, Napoli C, Municin\u00f2 M, Paolisso G, Balestrieri ML, Marfella R (2021) Glycated ACE2 receptor in diabetes: open door for SARS-COV-2 entry in cardiomyocyte. Cardiovasc Diabetol 20:99. https:\/\/doi.org\/10.1186\/s12933-021-01286-7","journal-title":"Cardiovasc Diabetol"},{"key":"10164_CR38","doi-asserted-by":"publisher","first-page":"17996","DOI":"10.1074\/jbc.M311191200","volume":"279","author":"P Towler","year":"2004","unstructured":"Towler P, Staker B, Prasad SG, Menon S, Tang J, Parsons T, Ryan D, Fisher M, Williams D, Dales NA, Patane MA, Pantoliano MW (2004) ACE2 X-ray structures reveal a large hinge-bending motion important for inhibitor binding and catalysis. J Biol Chem 279:17996\u201318007. https:\/\/doi.org\/10.1074\/jbc.M311191200","journal-title":"J Biol Chem"},{"key":"10164_CR39","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/978-3-540-88452-1_7","volume-title":"Cholera toxins","author":"K Chaudhuri","year":"2009","unstructured":"Chaudhuri K, Chatterjee SN (2009) Cholera toxin (CT): structure. Cholera toxins. Springer, Berlin, pp 105\u2013123"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-023-10164-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-023-10164-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-023-10164-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T10:17:58Z","timestamp":1709115478000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-023-10164-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,28]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10164"],"URL":"https:\/\/doi.org\/10.1007\/s10930-023-10164-y","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-3121889\/v1","asserted-by":"object"}]},"ISSN":["1572-3887","1875-8355"],"issn-type":[{"value":"1572-3887","type":"print"},{"value":"1875-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,28]]},"assertion":[{"value":"10 October 2023","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 November 2023","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The authors declare that they have no conflicts of interest with the contents of this article.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}