{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T21:18:46Z","timestamp":1771535926245,"version":"3.50.1"},"reference-count":102,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1007\/s10930-025-10265-w","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T16:28:32Z","timestamp":1744388912000},"page":"437-463","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Unveiling Prospective Therapeutic Potential of Conserved Hypothetical Plasmodium falciparum Proteins by Using Integrated Proteo Genomic Annotation and In-Silico Therapeutic Discovery Approach"],"prefix":"10.1007","volume":"44","author":[{"given":"Mamta","family":"Panda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varshita","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satyendra","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhaneswar","family":"Prusty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"issue":"10","key":"10265_CR1","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1038\/s41573-023-00772-9","volume":"22","author":"JL Siqueira-Neto","year":"2023","unstructured":"Siqueira-Neto JL et al (2023) Antimalarial drug discovery: progress and approaches. Nat Rev Drug Discov 22(10):807\u2013826","journal-title":"Nat Rev Drug Discov"},{"issue":"1","key":"10265_CR2","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1186\/s41182-023-00516-w","volume":"51","author":"AA El-Moamly","year":"2023","unstructured":"El-Moamly AA, El-Sweify MA (2023) Malaria vaccines: the 60-year journey of hope and final success-lessons learned and future prospects. Trop Med Health 51(1):29","journal-title":"Trop Med Health"},{"issue":"1","key":"10265_CR3","doi-asserted-by":"publisher","first-page":"2678","DOI":"10.1038\/s41467-022-30133-w","volume":"13","author":"P Jagannathan","year":"2022","unstructured":"Jagannathan P, Kakuru A (2022) Malaria in 2022: increasing challenges, cautious optimism. Nat Commun 13(1):2678","journal-title":"Nat Commun"},{"key":"10265_CR4","unstructured":"WHO, World malaria report. 23 December 2023"},{"key":"10265_CR5","unstructured":"World Malaria Report 2024 (2024)"},{"issue":"2","key":"10265_CR6","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1007\/s40471-021-00266-5","volume":"8","author":"MM Ippolito","year":"2021","unstructured":"Ippolito MM et al (2021) Antimalarial drug resistance and implications for the WHO global technical strategy. Curr Epidemiol Rep 8(2):46\u201362","journal-title":"Curr Epidemiol Rep"},{"issue":"1","key":"10265_CR7","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1186\/s12936-022-04115-8","volume":"21","author":"CV Plowe","year":"2022","unstructured":"Plowe CV (2022) Malaria chemoprevention and drug resistance: a review of the literature and policy implications. Malar J 21(1):104","journal-title":"Malar J"},{"issue":"10","key":"10265_CR8","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1038\/s41573-023-00772-9","volume":"22","author":"JL Siqueira-Neto","year":"2023","unstructured":"Siqueira-Neto JL et al (2023) Antimalarial drug discovery: progress and approaches. Nat Rev Drug Discov 22(10):807\u2013826","journal-title":"Nat Rev Drug Discovery"},{"key":"10265_CR9","unstructured":"Fernando SD, Climate change and Malaria\u2014A complex relationship. Welcome to United Nation"},{"key":"10265_CR10","unstructured":"Global Malaria Programme. May 2015 and updated in 2021"},{"issue":"6906","key":"10265_CR11","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/nature01097","volume":"419","author":"MJ Gardner","year":"2002","unstructured":"Gardner MJ et al (2002) Genome sequence of the human malaria parasite plasmodium falciparum. Nature 419(6906):498\u2013511","journal-title":"Nature"},{"issue":"9","key":"10265_CR12","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1038\/s41576-020-0244-x","volume":"21","author":"AK Cain","year":"2020","unstructured":"Cain AK et al (2020) A decade of advances in transposon-insertion sequencing. Nat Rev Genet 21(9):526\u2013540","journal-title":"Nat Rev Genet"},{"key":"10265_CR13","doi-asserted-by":"crossref","unstructured":"Zhang M et al (2018) Uncovering the essential genes of the human malaria parasite plasmodium falciparum by saturation mutagenesis. Science 360(6388)","DOI":"10.1126\/science.aap7847"},{"key":"10265_CR14","doi-asserted-by":"crossref","unstructured":"de Cr\u00e9cy-Lagard V et al (2022) A roadmap for the functional annotation of protein families: a community perspective. Database (Oxford) 2022","DOI":"10.1093\/database\/baac062"},{"issue":"1","key":"10265_CR15","doi-asserted-by":"publisher","first-page":"4654","DOI":"10.1038\/s41467-023-40365-z","volume":"14","author":"L Van den Broeck","year":"2023","unstructured":"Van den Broeck L et al (2023) Functional annotation of proteins for signaling network inference in non-model species. Nat Commun 14(1):4654","journal-title":"Nat Commun"},{"issue":"1","key":"10265_CR16","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/s12864-023-09924-y","volume":"25","author":"D Svedberg","year":"2024","unstructured":"Svedberg D et al (2024) Functional annotation of a divergent genome using sequence and structure-based similarity. BMC Genom 25(1):6","journal-title":"BMC Genomics"},{"issue":"Database issue","key":"10265_CR17","doi-asserted-by":"publisher","first-page":"D211","DOI":"10.1093\/nar\/gkp985","volume":"38","author":"RD Finn","year":"2010","unstructured":"Finn RD et al (2010) The Pfam protein families database. Nucleic Acids Res 38(Database issue):D211\u2013D222","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"10265_CR18","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1042\/EBC20200108","volume":"66","author":"R Morris","year":"2022","unstructured":"Morris R, Black KA, Stollar EJ (2022) Uncovering protein function: from classification to complexes. Essays Biochem 66(3):255\u2013285","journal-title":"Essays Biochem"},{"issue":"10","key":"10265_CR19","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1101\/gr.278202","volume":"12","author":"LY Geer","year":"2002","unstructured":"Geer LY et al (2002) CDART: protein homology by domain architecture. Genome Res 12(10):1619\u20131623","journal-title":"Genome Res"},{"issue":"9","key":"10265_CR20","doi-asserted-by":"publisher","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","volume":"30","author":"P Jones","year":"2014","unstructured":"Jones P et al (2014) InterProScan 5: genome-scale protein function classification. Bioinformatics 30(9):1236\u20131240","journal-title":"Bioinformatics"},{"issue":"6","key":"10265_CR21","doi-asserted-by":"publisher","first-page":"e99368","DOI":"10.1371\/journal.pone.0099368","volume":"9","author":"CS Yu","year":"2014","unstructured":"Yu CS et al (2014) CELLO2GO: a web server for protein subcellular localization prediction with functional gene ontology annotation. PLoS ONE 9(6):e99368","journal-title":"PLoS ONE"},{"key":"10265_CR22","doi-asserted-by":"crossref","unstructured":"Horton P et al (2007) WoLF PSORT: protein localization predictor. Nucleic Acids Res 35(Web Server issue):W585-7.","DOI":"10.1093\/nar\/gkm259"},{"issue":"2","key":"10265_CR23","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1038\/nprot.2007.494","volume":"3","author":"KC Chou","year":"2008","unstructured":"Chou KC, Shen HB (2008) Cell-PLoc: a package of web servers for predicting subcellular localization of proteins in various organisms. Nat Protoc 3(2):153\u2013162","journal-title":"Nat Protoc"},{"issue":"4","key":"10265_CR24","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1006\/jmbi.2000.3903","volume":"300","author":"O Emanuelsson","year":"2000","unstructured":"Emanuelsson O et al (2000) Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300(4):1005\u20131016","journal-title":"J Mol Biol"},{"issue":"3","key":"10265_CR25","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","volume":"305","author":"A Krogh","year":"2001","unstructured":"Krogh A et al (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305(3):567\u2013580","journal-title":"J Mol Biol"},{"key":"10265_CR26","doi-asserted-by":"publisher","first-page":"e12019","DOI":"10.7717\/peerj.12019","volume":"9","author":"TTD Vu","year":"2021","unstructured":"Vu TTD, Jung J (2021) Protein function prediction with gene ontology: from traditional to deep learning models. PeerJ 9:e12019","journal-title":"PeerJ"},{"issue":"9","key":"10265_CR27","doi-asserted-by":"publisher","first-page":"2129","DOI":"10.1101\/gr.772403","volume":"13","author":"PD Thomas","year":"2003","unstructured":"Thomas PD et al (2003) PANTHER: a library of protein families and subfamilies indexed by function. Genome Res 13(9):2129\u20132141","journal-title":"Genome Res"},{"issue":"D1","key":"10265_CR28","doi-asserted-by":"publisher","first-page":"D607","DOI":"10.1093\/nar\/gky1131","volume":"47","author":"D Szklarczyk","year":"2019","unstructured":"Szklarczyk D et al (2019) STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Res 47(D1):D607\u2013d613","journal-title":"Nucleic Acids Res"},{"issue":"18","key":"10265_CR29","doi-asserted-by":"publisher","first-page":"3442","DOI":"10.1093\/nar\/28.18.3442","volume":"28","author":"B Snel","year":"2000","unstructured":"Snel B et al (2000) STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene. Nucleic Acids Res 28(18):3442\u20133444","journal-title":"Nucleic Acids Res"},{"issue":"13","key":"10265_CR30","doi-asserted-by":"publisher","first-page":"3784","DOI":"10.1093\/nar\/gkg563","volume":"31","author":"E Gasteiger","year":"2003","unstructured":"Gasteiger E et al (2003) ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res 31(13):3784\u20133788","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"10265_CR31","doi-asserted-by":"publisher","first-page":"100230","DOI":"10.1016\/j.jacig.2024.100230","volume":"3","author":"E Sharma","year":"2024","unstructured":"Sharma E, Vitte J (2024) A systematic review of allergen cross-reactivity: translating basic concepts into clinical relevance. J Allergy Clin Immunol Glob 3(2):100230","journal-title":"J Allergy Clin Immunol Glob"},{"issue":"13","key":"10265_CR32","doi-asserted-by":"publisher","first-page":"3381","DOI":"10.1093\/nar\/gkg520","volume":"31","author":"T Schwede","year":"2003","unstructured":"Schwede T et al (2003) SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 31(13):3381\u20133385","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"10265_CR33","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1038\/nprot.2015.053","volume":"10","author":"LA Kelley","year":"2015","unstructured":"Kelley LA et al (2015) The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc 10(6):845\u2013858","journal-title":"Nat Protoc"},{"issue":"10","key":"10265_CR34","doi-asserted-by":"publisher","first-page":"2525","DOI":"10.1016\/j.bpj.2011.10.024","volume":"101","author":"D Xu","year":"2011","unstructured":"Xu D, Zhang Y (2011) Improving the physical realism and structural accuracy of protein models by a two-step atomic-level energy minimization. Biophys J 101(10):2525\u20132534","journal-title":"Biophys J"},{"key":"10265_CR35","doi-asserted-by":"crossref","unstructured":"Wiederstein M, Sippl MJ (2007) ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Res 35(Web Server issue):W407\u2013W410","DOI":"10.1093\/nar\/gkm290"},{"issue":"W1","key":"10265_CR36","doi-asserted-by":"publisher","first-page":"W436","DOI":"10.1093\/nar\/gkv462","volume":"43","author":"HA Hussein","year":"2015","unstructured":"Hussein HA et al (2015) PockDrug-Server: a new web server for predicting pocket druggability on holo and Apo proteins. Nucleic Acids Res 43(W1):W436\u2013W442","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"10265_CR37","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1007\/s11030-023-10636-4","volume":"28","author":"V Srivastava","year":"2024","unstructured":"Srivastava V et al (2024) Identification of FDA-approved drugs with triple targeting mode of action for the treatment of Monkeypox: a high throughput virtual screening study. Mol Divers 28(3):1093\u20131107","journal-title":"Mol Diversity"},{"issue":"14","key":"10265_CR38","doi-asserted-by":"publisher","first-page":"7384","DOI":"10.1080\/07391102.2023.2240415","volume":"42","author":"B Naik","year":"2024","unstructured":"Naik B et al (2024) Structure-based virtual screening approach reveals natural multi-target compounds for the development of antimalarial drugs to combat drug resistance. J Biomol Struct Dyn 42(14):7384\u20137408","journal-title":"J Biomol Struct Dynamics"},{"key":"10265_CR39","unstructured":"Glide S (2024) LLC, New York, NY, Glide, Schr\u00f6dinger. Schr\u00f6dinger Release 2024-3"},{"key":"10265_CR40","doi-asserted-by":"crossref","unstructured":"Godara P et al (2023) Structure-based virtual screening against multiple plasmodium falciparum kinases reveals antimalarial compounds. Mol Divers","DOI":"10.1007\/s11030-023-10770-z"},{"key":"10265_CR41","doi-asserted-by":"publisher","first-page":"121121","DOI":"10.1016\/j.lfs.2022.121121","volume":"311","author":"P Godara","year":"2022","unstructured":"Godara P et al (2022) Rational designing of peptide-ligand conjugates-based immunotherapy for the treatment of complicated malaria. Life Sci 311:121121","journal-title":"Life Sci"},{"issue":"6","key":"10265_CR42","doi-asserted-by":"publisher","first-page":"2278","DOI":"10.1007\/s00894-014-2278-5","volume":"20","author":"I Dimitrov","year":"2014","unstructured":"Dimitrov I et al (2014) AllerTOP v.2\u2013a server for in Silico prediction of allergens. J Mol Model 20(6):2278","journal-title":"J Mol Model"},{"issue":"1","key":"10265_CR43","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1471-2105-8-4","volume":"8","author":"IA Doytchinova","year":"2007","unstructured":"Doytchinova IA, Flower DR (2007) VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinform 8(1):4","journal-title":"BMC Bioinformatics"},{"issue":"1","key":"10265_CR44","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/1745-6150-8-27","volume":"8","author":"S Gupta","year":"2013","unstructured":"Gupta S et al (2013) Identification of B-cell epitopes in an antigen for inducing specific class of antibodies. Biol Direct 8(1):27","journal-title":"Biol Direct"},{"issue":"5","key":"10265_CR45","doi-asserted-by":"publisher","first-page":"2685","DOI":"10.1128\/IAI.68.5.2685-2691.2000","volume":"68","author":"M Parra","year":"2000","unstructured":"Parra M et al (2000) Characterization of conserved T- and B-cell epitopes in plasmodium falciparum major merozoite surface protein 1. Infect Immun 68(5):2685\u20132691","journal-title":"Infect Immun"},{"key":"10265_CR46","doi-asserted-by":"publisher","first-page":"594653","DOI":"10.3389\/fimmu.2020.594653","volume":"11","author":"SJ Gonzales","year":"2020","unstructured":"Gonzales SJ et al (2020) Naturally acquired humoral immunity against plasmodium falciparum malaria. Front Immunol 11:594653","journal-title":"Front Immunol"},{"issue":"1","key":"10265_CR47","first-page":"40","volume":"65","author":"S Saha","year":"2006","unstructured":"Saha S, Raghava GPS (2006) Prediction of continuous B-cell epitopes in an antigen using recurrent neural network. Proteins Struct Funct Bioinform 65(1):40\u201348","journal-title":"Prediction Continuous B-cell Epitopes Antigen Using Recurr Neural Netw"},{"key":"10265_CR48","doi-asserted-by":"crossref","unstructured":"Ojha R et al (2023) Multi-pathogen based chimeric vaccine to fight against COVID-19 and concomitant coinfections. 45(7):779\u2013797","DOI":"10.1007\/s10529-023-03380-0"},{"key":"10265_CR49","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1471-2105-8-4","volume":"8","author":"IA Doytchinova","year":"2007","unstructured":"Doytchinova IA, Flower DR (2007) VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinform 8:4","journal-title":"BMC Bioinformatics"},{"issue":"1","key":"10265_CR50","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1186\/1475-2875-7-186","volume":"7","author":"FMS Leoratti","year":"2008","unstructured":"Leoratti FMS et al (2008) Pattern of humoral immune response to plasmodium falciparum blood stages in individuals presenting different clinical expressions of malaria. Malar J 7(1):186","journal-title":"Malar J"},{"issue":"47","key":"10265_CR51","doi-asserted-by":"publisher","first-page":"eadj6367","DOI":"10.1126\/sciadv.adj6367","volume":"9","author":"JB Nilsson","year":"2023","unstructured":"Nilsson JB et al (2023) Accurate prediction of HLA class II antigen presentation across all loci using tailored data acquisition and refined machine learning. Sci Adv 9(47):eadj6367","journal-title":"Sci Adv"},{"key":"10265_CR52","doi-asserted-by":"crossref","unstructured":"Ojha R et al (2020) High throughput and comprehensive approach to develop multiepitope vaccine against minacious COVID-19. 151:105375","DOI":"10.1016\/j.ejps.2020.105375"},{"key":"10265_CR53","doi-asserted-by":"crossref","unstructured":"Reynisson B et al (2020) NetMHCpan-4.1 and NetMHCIIpan-4.0: improved predictions of MHC antigen presentation by concurrent motif deconvolution and integration of MS MHC eluted ligand data. 48(W1):W449-W454","DOI":"10.1093\/nar\/gkaa379"},{"key":"10265_CR54","first-page":"187","volume":"189","author":"SL Hoffman","year":"1994","unstructured":"Hoffman SL, Sedegah M, Malik A (1994) Cytotoxic T lymphocytes in humans exposed to plasmodium falciparum by immunization or natural exposure. Curr Top Microbiol Immunol 189:187\u2013203","journal-title":"Curr Top Microbiol Immunol"},{"issue":"11","key":"10265_CR55","doi-asserted-by":"publisher","first-page":"5499","DOI":"10.4049\/jimmunol.168.11.5499","volume":"168","author":"B Livingston","year":"2002","unstructured":"Livingston B et al (2002) A rational strategy to design multiepitope immunogens based on multiple Th lymphocyte epitopes. J Immunol 168(11):5499\u20135506","journal-title":"J Immunol"},{"issue":"3","key":"10265_CR56","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1080\/21645515.2015.1012017","volume":"11","author":"Y Yang","year":"2015","unstructured":"Yang Y et al (2015) In Silico design of a DNA-based HIV-1 multi-epitope vaccine for Chinese populations. Hum Vaccin Immunother 11(3):795\u2013805","journal-title":"Hum Vaccin Immunother"},{"key":"10265_CR57","doi-asserted-by":"crossref","unstructured":"Singh S et al (2022) High-throughput virtual screening of small-molecule inhibitors targeting immune cell checkpoints to discover new immunotherapeutics for human diseases. 1\u201323","DOI":"10.1007\/s11030-022-10452-2"},{"key":"10265_CR58","doi-asserted-by":"crossref","unstructured":"Larsen MV et al (2007) Large-scale validation of methods for cytotoxic T-lymphocyte epitope prediction. 8:1\u201312","DOI":"10.1186\/1471-2105-8-424"},{"key":"10265_CR59","doi-asserted-by":"crossref","unstructured":"Singh S et al (2024) From genome to clinic: the power of translational bioinformatics in improving human health. 139:1\u201325","DOI":"10.1016\/bs.apcsb.2023.11.010"},{"key":"10265_CR60","doi-asserted-by":"crossref","unstructured":"Li X et al (2016) Design and evaluation of a multi-epitope peptide of human metapneumovirus. 58(6):403\u2013412","DOI":"10.1159\/000445059"},{"issue":"6","key":"10265_CR61","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1159\/000445059","volume":"58","author":"X Li","year":"2015","unstructured":"Li X et al (2015) Design and evaluation of a Multi-Epitope peptide of human metapneumovirus. Intervirology 58(6):403\u2013412","journal-title":"Intervirology"},{"issue":"1","key":"10265_CR62","doi-asserted-by":"publisher","first-page":"24485","DOI":"10.1038\/s41598-021-03932-2","volume":"11","author":"H Tarrahimofrad","year":"2021","unstructured":"Tarrahimofrad H et al (2021) Designing a multi-epitope vaccine to provoke the robust immune response against influenza A H7N9. Sci Rep 11(1):24485","journal-title":"Sci Rep"},{"key":"10265_CR63","doi-asserted-by":"crossref","unstructured":"Singh S et al (2023) Translational vaccinomics and structural filtration algorithm to device multiepitope vaccine for catastrophic monkeypox virus. 153:106497","DOI":"10.1016\/j.compbiomed.2022.106497"},{"key":"10265_CR64","doi-asserted-by":"crossref","unstructured":"Kupani M et al (2023) Prediction of an Immunogenic peptide ensemble and multi-subunit vaccine for visceral leishmaniasis using bioinformatics approaches. 9(12)","DOI":"10.1016\/j.heliyon.2023.e22121"},{"key":"10265_CR65","doi-asserted-by":"crossref","unstructured":"Rosa DS et al (2004) The Pan HLA DR-binding epitope improves adjuvant-assisted immunization with a Recombinant protein containing a malaria vaccine candidate. 92(3):259\u2013268","DOI":"10.1016\/j.imlet.2004.01.006"},{"key":"10265_CR66","doi-asserted-by":"crossref","unstructured":"Sharma N et al (2022) ToxinPred2: an improved method for predicting toxicity of proteins. 23(5):bbac174","DOI":"10.1093\/bib\/bbac174"},{"key":"10265_CR67","doi-asserted-by":"crossref","unstructured":"Sharma V et al (2022) Immunoinformatics and reverse vaccinology methods to design peptide-based vaccines. In: Advances in protein molecular and structural biology methods. Elsevier, pp 477\u2013487","DOI":"10.1016\/B978-0-323-90264-9.00029-5"},{"key":"10265_CR68","doi-asserted-by":"crossref","unstructured":"Gasteiger E et al (2005) Protein identification and analysis tools on the expasy server. Springer","DOI":"10.1385\/1-59259-890-0:571"},{"issue":"12","key":"10265_CR69","doi-asserted-by":"publisher","first-page":"1911","DOI":"10.1002\/prot.26211","volume":"89","author":"Y Li","year":"2021","unstructured":"Li Y et al (2021) Protein inter-residue contact and distance prediction by coupling complementary Coevolution features with deep residual networks in CASP14. Proteins 89(12):1911\u20131921","journal-title":"Proteins"},{"key":"10265_CR70","doi-asserted-by":"crossref","unstructured":"Xu D, Zhang YJBj (2011) Improving the physical realism and structural accuracy of protein models by a two-step atomic-level energy minimization. 101(10):2525\u20132534","DOI":"10.1016\/j.bpj.2011.10.024"},{"key":"10265_CR71","doi-asserted-by":"crossref","unstructured":"Heo L, Park H, Seok CJNar (2013) GalaxyRefine: protein structure refinement driven by side-chain repacking. 41(W1):W384-W388","DOI":"10.1093\/nar\/gkt458"},{"key":"10265_CR72","doi-asserted-by":"crossref","unstructured":"Singh S, Rao A, Verma SK (2023) Need for quicker systems for development and evaluation of vaccines and Drugs. In: Preparedness for future pandemics: threats and challenges. Springer, pp 105\u2013120","DOI":"10.1007\/978-981-99-3201-6_9"},{"key":"10265_CR73","doi-asserted-by":"crossref","unstructured":"Rao A et al (2024) Quantum-Enabled Drug Discovery Process. 27\u201355","DOI":"10.1002\/9781394159338.ch2"},{"issue":"2","key":"10265_CR74","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/nprot.2016.169","volume":"12","author":"D Kozakov","year":"2017","unstructured":"Kozakov D et al (2017) The cluspro web server for protein\u2013protein Docking. Nat Protoc 12(2):255\u2013278","journal-title":"Nat Protoc"},{"key":"10265_CR75","doi-asserted-by":"crossref","unstructured":"Yan Y et al (2017) HDOCK: a web server for protein\u2013protein and protein\u2013DNA\/RNA Docking based on a hybrid strategy. 45(W1):W365\u2013W373","DOI":"10.1093\/nar\/gkx407"},{"key":"10265_CR76","doi-asserted-by":"crossref","unstructured":"Rose PW et al (2012) The RCSB Protein Data Bank: new resources for research and education. 41(D1):D475-D482","DOI":"10.1093\/nar\/gks1200"},{"key":"10265_CR77","doi-asserted-by":"crossref","unstructured":"Singh S, Prajapati VKJB (2022) Exploring actinomycetes natural products to identify potential multi-target inhibitors against Leishmania donovani. 12(9):235","DOI":"10.1007\/s13205-022-03304-1"},{"key":"10265_CR78","doi-asserted-by":"crossref","unstructured":"Singh S et al (2023) Identification of multi-targeting natural antiviral peptides to impede SARS-CoV-2 infection. 34(5):1743\u20131758","DOI":"10.1007\/s11224-022-02113-9"},{"key":"10265_CR79","doi-asserted-by":"crossref","unstructured":"Singh S et al (2023) High-throughput virtual screening of small-molecule inhibitors targeting immune cell checkpoints to discover new immunotherapeutics for human diseases. 27(2):729\u2013751","DOI":"10.1007\/s11030-022-10452-2"},{"key":"10265_CR80","doi-asserted-by":"crossref","unstructured":"Singh S et al (2023) Multifaceted mutational immunotherapeutic approach to design therapeutic mAbs to combat monkeypox disease via integrated screening algorithms and antibody engineering. 8(10):1301\u20131318","DOI":"10.1039\/D3ME00059A"},{"key":"10265_CR81","doi-asserted-by":"crossref","unstructured":"Singh S et al (2022) Translational vaccinomics and structural filtration algorithm to device multiepitope vaccine for catastrophic monkeypox virus. 106497","DOI":"10.1016\/j.compbiomed.2022.106497"},{"key":"10265_CR82","unstructured":"Bekker H et al (1993) Gromacs-a parallel computer for molecular-dynamics simulations. In: 4th international conference on computational physics (PC 92). World Scientific Publishing"},{"issue":"4","key":"10265_CR83","first-page":"e9862","volume":"5","author":"N Rapin","year":"2010","unstructured":"Rapin N et al (2010) Computational immunology Meets bioinformatics: the use of prediction tools for. Mol Bind Simul Immune Syst 5(4):e9862","journal-title":"Mol Binding Simul Immune Syst"},{"key":"10265_CR84","doi-asserted-by":"crossref","unstructured":"Stolfi P et al (2022) In-silico evaluation of adenoviral COVID-19 vaccination protocols: assessment of immunological memory up to 6 months after the third dose. 13:998262","DOI":"10.3389\/fimmu.2022.998262"},{"key":"10265_CR85","doi-asserted-by":"crossref","unstructured":"Moodley A et al (2022) Reverse vaccinology approach to design a multi-epitope vaccine construct based on the Mycobacterium tuberculosis biomarker PE_PGRS17. 70(4):501\u2013517","DOI":"10.1007\/s12026-022-09284-x"},{"key":"10265_CR86","doi-asserted-by":"crossref","unstructured":"Shilling PJ et al (2020) Improved designs for pET expression plasmids increase protein production yield in Escherichia coli. 3(1):214","DOI":"10.1038\/s42003-020-0939-8"},{"issue":"1","key":"10265_CR87","doi-asserted-by":"publisher","first-page":"a004978","DOI":"10.1101\/cshperspect.a004978","volume":"3","author":"S Ricard-Blum","year":"2011","unstructured":"Ricard-Blum S (2011) The collagen family. Cold Spring Harb Perspect Biol 3(1):a004978","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"10","key":"10265_CR88","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1016\/j.pt.2022.08.002","volume":"38","author":"OA Akuh","year":"2022","unstructured":"Akuh OA et al (2022) The ferredoxin redox system - an essential electron distributing hub in the apicoplast of apicomplexa. Trends Parasitol 38(10):868\u2013881","journal-title":"Trends Parasitol"},{"key":"10265_CR89","doi-asserted-by":"crossref","unstructured":"Chen L et al (2021) Bioinformatics and transcriptome analysis of CFEM proteins in fusarium graminearum. J Fungi (Basel) 7(10)","DOI":"10.3390\/jof7100871"},{"issue":"9","key":"10265_CR90","doi-asserted-by":"publisher","first-page":"749","DOI":"10.3390\/pathogens9090749","volume":"9","author":"J Regidor-Cerrillo","year":"2020","unstructured":"Regidor-Cerrillo J et al (2020) Proteomic characterization of Host-Pathogen interactions during bovine trophoblast cell line infection by neospora Caninum. Pathogens 9(9):749","journal-title":"Pathogens"},{"issue":"5","key":"10265_CR91","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1007\/s10930-018-9785-7","volume":"37","author":"BP Jain","year":"2018","unstructured":"Jain BP, Pandey S (2018) WD40 repeat proteins: signalling scaffold with diverse functions. Protein J 37(5):391\u2013406","journal-title":"Protein J"},{"key":"10265_CR92","doi-asserted-by":"publisher","first-page":"2054","DOI":"10.3389\/fmicb.2020.02054","volume":"11","author":"P Speziale","year":"2020","unstructured":"Speziale P, Pietrocola G (2020) The multivalent role of Fibronectin-Binding proteins A and B (FnBPA and FnBPB) of Staphylococcus aureus in host infections. Front Microbiol 11:2054","journal-title":"Front Microbiol"},{"issue":"4","key":"10265_CR93","doi-asserted-by":"publisher","first-page":"360","DOI":"10.4161\/rna.28302","volume":"11","author":"AM Burroughs","year":"2014","unstructured":"Burroughs AM, Aravind L (2014) A highly conserved family of domains related to the DNA-glycosylase fold helps predict multiple novel pathways for RNA modifications. RNA Biol 11(4):360\u2013372","journal-title":"RNA Biol"},{"issue":"9","key":"10265_CR94","doi-asserted-by":"publisher","first-page":"3937","DOI":"10.1128\/iai.62.9.3937-3946.1994","volume":"62","author":"HS Courtney","year":"1994","unstructured":"Courtney HS et al (1994) Cloning, sequencing, and expression of a fibronectin\/fibrinogen-binding protein from group A Streptococci. Infect Immun 62(9):3937\u20133946","journal-title":"Infect Immun"},{"issue":"D1","key":"10265_CR95","doi-asserted-by":"publisher","first-page":"D605","DOI":"10.1093\/nar\/gkaa1074","volume":"49","author":"D Szklarczyk","year":"2021","unstructured":"Szklarczyk D et al (2021) The STRING database in 2021: customizable protein-protein networks, and functional characterization of user-uploaded gene\/measurement sets. Nucleic Acids Res 49(D1):D605\u2013d612","journal-title":"Nucleic Acids Res"},{"key":"10265_CR96","doi-asserted-by":"publisher","first-page":"129562","DOI":"10.1016\/j.ijbiomac.2024.129562","volume":"260","author":"V Srivastava","year":"2024","unstructured":"Srivastava V et al (2024) Peptide-ligand conjugate based immunotherapeutic approach for targeted dismissal of non-structural protein 1 of dengue virus: a novel therapeutic solution for mild and severe dengue infections. Int J Biol Macromol 260:129562","journal-title":"Int J Biol Macromol"},{"key":"10265_CR97","doi-asserted-by":"crossref","unstructured":"Bhalerao P et al (2024) Exploring malaria parasite surface proteins to devise highly Immunogenic multi-epitope subunit vaccine for plasmodium falciparum. 22(2):100377","DOI":"10.1016\/j.jgeb.2024.100377"},{"key":"10265_CR98","doi-asserted-by":"crossref","unstructured":"Mogensen TH (2009) Pathogen recognition and inflammatory signaling in innate immune defenses. Clin Microbiol Rev 22(2):240\u201373, Table of Contents.","DOI":"10.1128\/CMR.00046-08"},{"key":"10265_CR99","doi-asserted-by":"crossref","unstructured":"Bonam SR et al (2021) Plasmodium falciparum malaria vaccines and vaccine adjuvants. Vaccines (Basel) 9(10)","DOI":"10.3390\/vaccines9101072"},{"key":"10265_CR100","doi-asserted-by":"publisher","first-page":"740117","DOI":"10.3389\/fimmu.2021.740117","volume":"12","author":"AB Enriquez","year":"2021","unstructured":"Enriquez AB et al (2021) Advancing adjuvants for Mycobacterium tuberculosis therapeutics. Front Immunol 12:740117","journal-title":"Front Immunol"},{"key":"10265_CR101","doi-asserted-by":"publisher","first-page":"1249718","DOI":"10.3389\/fmicb.2023.1249718","volume":"14","author":"MEH Kayesh","year":"2023","unstructured":"Kayesh MEH, Kohara M, Tsukiyama-Kohara K (2023) TLR agonists as vaccine adjuvants in the prevention of viral infections: an overview. Front Microbiol 14:1249718","journal-title":"Front Microbiol"},{"key":"10265_CR102","doi-asserted-by":"crossref","unstructured":"Singh S et al (2024) Technological advancements in viral vector designing and optimization for therapeutic applications. 139:57\u201387","DOI":"10.1016\/bs.apcsb.2023.11.013"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-025-10265-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-025-10265-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-025-10265-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T13:59:29Z","timestamp":1757167169000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-025-10265-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,11]]},"references-count":102,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10265"],"URL":"https:\/\/doi.org\/10.1007\/s10930-025-10265-w","relation":{},"ISSN":["1572-3887","1875-8355"],"issn-type":[{"value":"1572-3887","type":"print"},{"value":"1875-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,11]]},"assertion":[{"value":"17 March 2025","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 April 2025","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}