{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:52:40Z","timestamp":1791010360980,"version":"4.1.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Protein J"],"DOI":"10.1007\/s10930-026-10350-8","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:24:53Z","timestamp":1785515093000},"page":"735-744","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression, Purification, and Characterization of Recombinant Isocitrate Dehydrogenase (IDH) from Yarrowia lipolytica and its Application as a Protein Template for In Situ Synthesis of Gold Nanoparticles"],"prefix":"10.1007","volume":"45","author":[{"given":"Fatemeh","family":"Mojahedian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Karimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farangis","family":"Ataei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saman","family":"Hosseinkhani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"issue":"1","key":"10350_CR1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1038\/s43246-024-00557-6","volume":"5","author":"S Khumngern","year":"2024","unstructured":"Khumngern S, Jeerapan I (2024) Synergistic convergence of materials and enzymes for biosensing and self-sustaining energy devices towards on-body health monitoring. Commun Mater 5(1):135","journal-title":"Commun Mater"},{"issue":"5","key":"10350_CR2","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.3390\/nano11051156","volume":"11","author":"W Lipi\u0144ska","year":"2021","unstructured":"Lipi\u0144ska W, Grochowska K, Siuzdak K (2021) Enzyme immobilization on gold nanoparticles for electrochemical glucose biosensors. Nanomaterials 11(5):1156","journal-title":"Nanomaterials"},{"issue":"3","key":"10350_CR3","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1007\/s10930-024-10202-3","volume":"43","author":"M Nabi Afjadi","year":"2024","unstructured":"Nabi Afjadi M, Yazdanparast R, Barzegari E (2024) The impact of terminal peptide extensions of retinal inosine 5 monophosphate dehydrogenase 1 isoforms on their DNA-binding activities. Protein J 43(3):592\u2013602","journal-title":"Protein J"},{"issue":"10","key":"10350_CR4","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/s13205-021-02953-y","volume":"11","author":"K Thakur","year":"2021","unstructured":"Thakur K, Attri C, Seth A (2021) Nanocarriers-based immobilization of enzymes for industrial application. 3 Biotech 11(10):427","journal-title":"3 Biotech"},{"issue":"7","key":"10350_CR5","doi-asserted-by":"publisher","first-page":"2754","DOI":"10.1021\/jacs.8b10338","volume":"141","author":"R Chapman","year":"2019","unstructured":"Chapman R, Stenzel MH (2019) All wrapped up: stabilization of enzymes within single enzyme nanoparticles. J Am Chem Soc 141(7):2754\u20132769","journal-title":"J Am Chem Soc"},{"key":"10350_CR6","first-page":"1","volume":"2011","author":"X Li","year":"2011","unstructured":"Li X, Xu H, Chen Z-S, Chen G (2011) Biosynthesis of nanoparticles by microorganisms and their applications. J Nanomater 2011:1\u201316","journal-title":"J Nanomater"},{"issue":"1","key":"10350_CR7","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1089\/ind.2005.1.38","volume":"1","author":"F Xu","year":"2005","unstructured":"Xu F (2005) Applications of oxidoreductases: recent progress. Ind Biotechnol 1(1):38\u201350","journal-title":"Ind Biotechnol"},{"issue":"4","key":"10350_CR8","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/S0958-1669(99)80067-6","volume":"10","author":"SW May","year":"1999","unstructured":"May SW (1999) Applications of oxidoreductases. Curr Opin Biotechnol 10(4):370\u2013375","journal-title":"Curr Opin Biotechnol"},{"issue":"1","key":"10350_CR9","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1042\/BCJ20160699","volume":"474","author":"T Shimizu","year":"2017","unstructured":"Shimizu T, Yin L, Yoshida A, Yokooji Y, Hachisuka SI, Sato T, Tomita T, Nishida H, Atomi H, Kuzuyama T et al (2017) Structure and function of an ancestral-type beta-decarboxylating dehydrogenase from Thermococcus kodakarensis. Biochem J 474(1):105\u2013122","journal-title":"Biochem J"},{"issue":"8","key":"10350_CR10","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1007\/s00203-010-0595-3","volume":"192","author":"S Matsuo","year":"2010","unstructured":"Matsuo S, Shirai H, Takada Y (2010) Isocitrate dehydrogenase isozymes from a psychrotrophic bacterium, Pseudomonas psychrophila. Arch Microbiol 192(8):639\u2013650","journal-title":"Arch Microbiol"},{"issue":"1","key":"10350_CR11","doi-asserted-by":"publisher","first-page":"3208","DOI":"10.1038\/s41598-021-81751-1","volume":"11","author":"C Arib","year":"2021","unstructured":"Arib C, Spadavecchia J, de La Chapelle ML (2021) Enzyme mediated synthesis of hybrid polyedric gold nanoparticles. Sci Rep 11(1):3208","journal-title":"Sci Rep"},{"key":"10350_CR12","doi-asserted-by":"publisher","first-page":"1446355","DOI":"10.3389\/fbioe.2024.1446355","volume":"12","author":"T Jamshidnejad-Tosaramandani","year":"2024","unstructured":"Jamshidnejad-Tosaramandani T, Kashanian S, Omidfar K, Schi\u00f6th H (2024) Recent advances in gold nanostructure-based biosensors in detecting diabetes biomarkers. Front Bioeng Biotechnol 12:1446355","journal-title":"Front Bioeng Biotechnol"},{"issue":"1","key":"10350_CR13","doi-asserted-by":"publisher","first-page":"3932","DOI":"10.1101\/pdb.prot3932","volume":"2006","author":"J Sambrook","year":"2006","unstructured":"Sambrook J, Russell DW (2006) Preparation and transformation of competent E. coli using calcium chloride. Cold Spring Harbor Protoc 2006(1):pdb. prot3932","journal-title":"Cold Spring Harbor Protoc"},{"key":"10350_CR14","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s11010-019-03675-9","volume":"465","author":"B Andashti","year":"2020","unstructured":"Andashti B, Yazdanparast R, Barzegari E, Galehdari H (2020) The functional impact of the C\/N-terminal extensions of the mouse retinal IMPDH1 isoforms: a kinetic evaluation. Mol Cell Biochem 465:155\u2013164","journal-title":"Mol Cell Biochem"},{"key":"10350_CR15","doi-asserted-by":"publisher","first-page":"109424","DOI":"10.1016\/j.enzmictec.2019.109424","volume":"131","author":"H Salehi-Sedeh","year":"2019","unstructured":"Salehi-Sedeh H, Ataei F, Jarchi S, Hamidi R, Hosseinkhani S (2019) Effect of mutation at positively charged residues (K329 and R330) in a flexible region of firefly luciferase on structure and kinetic properties. Enzym Microb Technol 131:109424","journal-title":"Enzym Microb Technol"},{"issue":"6\u20137","key":"10350_CR16","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1002\/ijch.201400178","volume":"55","author":"Y Hu","year":"2015","unstructured":"Hu Y, Guo W, Wei H (2015) Protein-and peptide\u2010directed approaches to fluorescent metal nanoclusters. Isr J Chem 55(6\u20137):682\u2013697","journal-title":"Isr J Chem"},{"issue":"11","key":"10350_CR17","doi-asserted-by":"publisher","first-page":"918","DOI":"10.3390\/bios12110918","volume":"12","author":"E Karimi","year":"2022","unstructured":"Karimi E, Nikkhah M, Hosseinkhani S (2022) Label-free and bioluminescence-based nano-biosensor for ATP detection. Biosens (Basel) 12(11):918","journal-title":"Biosens (Basel)"},{"issue":"13\u201314","key":"10350_CR18","doi-asserted-by":"publisher","first-page":"2861","DOI":"10.1016\/j.saa.2004.10.035","volume":"61","author":"YQ He","year":"2005","unstructured":"He YQ, Liu SP, Kong L, Liu ZF (2005) A study on the sizes and concentrations of gold nanoparticles by spectra of absorption, resonance Rayleigh scattering and resonance non-linear scattering. Spectrochim Acta Mol Biomol Spectrosc 61(13\u201314):2861\u20132866","journal-title":"Spectrochim Acta Mol Biomol Spectrosc"},{"issue":"1","key":"10350_CR19","doi-asserted-by":"publisher","first-page":"21525","DOI":"10.1038\/s41598-023-48834-7","volume":"13","author":"O Matvieiev","year":"2023","unstructured":"Matvieiev O, \u0160ele\u0161ovsk\u00e1 R, Marton M, Hatala M, Metelka R, Weis M, Vojs M (2023) Effect of different modification by gold nanoparticles on the electrochemical performance of screen-printed sensors with boron-doped diamond electrode. Sci Rep 13(1):21525","journal-title":"Sci Rep"},{"issue":"6","key":"10350_CR20","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1158\/2159-8290.CD-18-1040","volume":"9","author":"D Chen","year":"2019","unstructured":"Chen D, Xia S, Wang M, Lin R, Li Y, Mao H, Aguiar M, Famulare CA, Shih AH, Brennan CW (2019) Mutant and wild-type isocitrate dehydrogenase 1 share enhancing mechanisms involving distinct tyrosine kinase cascades in cancer. Cancer Discov 9(6):756\u2013777","journal-title":"Cancer Discov"},{"issue":"1","key":"10350_CR21","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1111\/j.1432-1033.1991.tb16347.x","volume":"202","author":"ML Leyland","year":"1991","unstructured":"Leyland ML, Kelly DJ (1991) Purification and characterization of a monomeric isocitrate dehydrogenase with dual coenzyme specificity from the photosynthetic bacterium Rhodomicrobium vannielii. Eur J Biochem 202(1):85\u201393","journal-title":"Eur J Biochem"},{"issue":"2","key":"10350_CR22","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1111\/j.1574-6968.2011.02467.x","volume":"327","author":"P Wang","year":"2012","unstructured":"Wang P, Jin M, Zhu G (2012) Biochemical and molecular characterization of NAD(+)-dependent isocitrate dehydrogenase from the ethanologenic bacterium Zymomonas mobilis. FEMS Microbiol Lett 327(2):134\u2013141","journal-title":"FEMS Microbiol Lett"},{"key":"10350_CR23","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.1016\/j.ijheatmasstransfer.2017.07.005","volume":"114","author":"M Chen","year":"2017","unstructured":"Chen M, He Y, Zhu J (2017) Preparation of Au\u2013Ag bimetallic nanoparticles for enhanced solar photothermal conversion. Int J Heat Mass Transf 114:1098\u20131104","journal-title":"Int J Heat Mass Transf"},{"key":"10350_CR24","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1016\/j.talanta.2018.02.088","volume":"184","author":"N Elahi","year":"2018","unstructured":"Elahi N, Kamali M, Baghersad MH (2018) Recent biomedical applications of gold nanoparticles: a review. Talanta 184:537\u2013556","journal-title":"Talanta"},{"key":"10350_CR25","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1007\/s12274-016-1264-x","volume":"10","author":"B Sun","year":"2017","unstructured":"Sun B, Wu J, Cui S, Zhu H, An W, Fu Q, Shao C, Yao A, Chen B, Shi D (2017) In situ synthesis of graphene oxide\/gold nanorods theranostic hybrids for efficient tumor computed tomography imaging and photothermal therapy. Nano Res 10:37\u201348","journal-title":"Nano Res"},{"issue":"12","key":"10350_CR26","doi-asserted-by":"publisher","first-page":"2061","DOI":"10.3390\/s16122061","volume":"16","author":"D Chang","year":"2016","unstructured":"Chang D, Zakaria S, Deng M, Allen N, Tram K, Li Y (2016) Integrating deoxyribozymes into colorimetric sensing platforms. Sensors 16(12):2061","journal-title":"Sensors"},{"issue":"21","key":"10350_CR27","doi-asserted-by":"publisher","first-page":"4240","DOI":"10.1039\/c2lc40741h","volume":"12","author":"L Shen","year":"2012","unstructured":"Shen L, Hagen JA, Papautsky I (2012) Point-of-care colorimetric detection with a smartphone. Lab Chip 12(21):4240\u20134243","journal-title":"Lab Chip"},{"issue":"36","key":"10350_CR28","doi-asserted-by":"publisher","first-page":"7460","DOI":"10.1039\/C4TC01142B","volume":"2","author":"L Polavarapu","year":"2014","unstructured":"Polavarapu L, P\u00e9rez-Juste J, Xu Q-H, Liz-Marz\u00e1n LM (2014) Optical sensing of biological, chemical and ionic species through aggregation of plasmonic nanoparticles. J Mater Chem C 2(36):7460\u20137476","journal-title":"J Mater Chem C"},{"issue":"10","key":"10350_CR29","doi-asserted-by":"publisher","first-page":"1370","DOI":"10.3390\/molecules21101370","volume":"21","author":"FB Rehm","year":"2016","unstructured":"Rehm FB, Chen S, Rehm BH (2016) Enzyme engineering for in situ immobilization. Molecules 21(10):1370","journal-title":"Molecules"},{"key":"10350_CR30","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.copbio.2015.04.005","volume":"34","author":"S Ding","year":"2015","unstructured":"Ding S, Cargill AA, Medintz IL, Claussen JC (2015) Increasing the activity of immobilized enzymes with nanoparticle conjugation. Curr Opin Biotechnol 34:242\u2013250","journal-title":"Curr Opin Biotechnol"},{"issue":"16","key":"10350_CR31","doi-asserted-by":"publisher","first-page":"5172","DOI":"10.1039\/C7NR00200A","volume":"9","author":"JN Vranish","year":"2017","unstructured":"Vranish JN, Ancona MG, Oh E, Susumu K, Medintz IL (2017) Enhancing coupled enzymatic activity by conjugating one enzyme to a nanoparticle. Nanoscale 9(16):5172\u20135187","journal-title":"Nanoscale"}],"container-title":["The Protein Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-026-10350-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10930-026-10350-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10930-026-10350-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:02:44Z","timestamp":1791007364000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10930-026-10350-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,31]]},"references-count":31,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["10350"],"URL":"https:\/\/doi.org\/10.1007\/s10930-026-10350-8","relation":{},"ISSN":["1875-8355"],"issn-type":[{"value":"1875-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,31]]},"assertion":[{"value":"30 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors have no conflict of interest to declare that are relevant to the content of this article..","order":1,"name":"Ethics","label":"Funding","group":{"name":"EthicsHeading","label":"Statements and Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Competing Interests","group":{"name":"EthicsHeading","label":"Statements and Declarations"}},{"value":"F. M. performed the experiment; E. K. and F. A. designed part of the experiments and read the manuscript. S. H. designed the work, wrote the manuscript, and granted the work. All authors have read and agreed to the published version of the manuscript.","order":3,"name":"Ethics","label":"<b>Author Contributions<\/b>","group":{"name":"EthicsHeading","label":"Statements and Declarations"}},{"value":"This is an observation study, The Research Ethics Committee of Tarbiat Modares University has confirmed that no ethical approval is required.This is an observation study, The Research Ethics Committee of Tarbiat Modares University has confirmed that no ethical approval is regqguired.This is an observational study. The XYZZ Research Ethics Committee of Tarbiat MmMmmodares University has confirmed that no ethical approval is required.\u201d\u201d\u201d\u201d","order":4,"name":"Ethics","label":"<b>Ethics approval<\/b>","group":{"name":"EthicsHeading","label":"Statements and Declarations"}},{"value":"The authors confirm that the data supporting the findings of this study are available within the article.","order":5,"name":"Ethics","label":"Data Availability","group":{"name":"EthicsHeading","label":"Statements and Declarations"}}]}}