{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T01:18:34Z","timestamp":1774574314831,"version":"3.50.1"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Cell Biochem"],"published-print":{"date-parts":[[2024,1]]},"DOI":"10.1007\/s11010-023-04711-5","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T06:15:52Z","timestamp":1680502552000},"page":"99-107","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Large DNA fragment knock-in and sequential gene editing in Plasmodium falciparum: a preliminary study using suicide-rescue-based CRISPR\/Cas9 system"],"prefix":"10.1007","volume":"479","author":[{"given":"Junnan","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Tong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minghong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Adams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,4,1]]},"reference":[{"key":"4711_CR1","first-page":"1","volume-title":"World Malaria Report 2021","author":"WHO","year":"2021","unstructured":"WHO (2021) World Malaria Report 2021. World Health Organization, Geneva, pp 1\u2013104"},{"key":"4711_CR2","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1038\/nm.4381","volume":"23","author":"B Blasco","year":"2017","unstructured":"Blasco B, Leroy D, Fidock DA (2017) Antimalarial drug resistance: linking Plasmodium falciparum parasite biology to the clinic. Nat Med 23:917\u2013928. https:\/\/doi.org\/10.1038\/nm.4381","journal-title":"Nat Med"},{"key":"4711_CR3","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1186\/s12936-022-04385-2","volume":"21","author":"FV Rahmasari","year":"2022","unstructured":"Rahmasari FV, Asih PBS, Dewayanti FK, Rotejanaprasert C, Charunwatthana P, Imwong M, Syafruddin D (2022) Drug resistance of Plasmodium falciparum and Plasmodium vivax isolates in Indonesia. Malar J 21:354. https:\/\/doi.org\/10.1186\/s12936-022-04385-2","journal-title":"Malar J"},{"key":"4711_CR4","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1038\/nmeth.4223","volume":"14","author":"J Birnbaum","year":"2017","unstructured":"Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T (2017) A genetic system to study Plasmodium falciparum protein function. Nat Methods 14:450\u2013456. https:\/\/doi.org\/10.1038\/nmeth.4223","journal-title":"Nat Methods"},{"key":"4711_CR5","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/nature01097","volume":"419","author":"MJ Gardner","year":"2002","unstructured":"Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, Carlton JM, Pain A, Nelson KE, Bowman S, Paulsen IT, James K, Eisen JA, Rutherford K, Salzberg SL, Craig A, Kyes S, Chan MS, Nene V, Shallom SJ, Suh B, Peterson J, Angiuoli S, Pertea M, Allen J, Selengut J, Haft D, Mather MW, Vaidya AB, Martin DM, Fairlamb AH, Fraunholz MJ, Roos DS, Ralph SA, McFadden GI, Cummings LM, Subramanian GM, Mungall C, Venter JC, Carucci DJ, Hoffman SL, Newbold C, Davis RW, Fraser CM, Barrell B (2002) Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419:498\u2013511. https:\/\/doi.org\/10.1038\/nature01097","journal-title":"Nature"},{"key":"4711_CR6","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.molbiopara.2013.01.001","volume":"187","author":"S Hasenkamp","year":"2013","unstructured":"Hasenkamp S, Merrick CJ, Horrocks P (2013) A quantitative analysis of Plasmodium falciparum transfection using DNA-loaded erythrocytes. Mol Biochem Parasitol 187:117\u2013120. https:\/\/doi.org\/10.1016\/j.molbiopara.2013.01.001","journal-title":"Mol Biochem Parasitol"},{"key":"4711_CR7","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1038\/nbt.2925","volume":"32","author":"M Ghorbal","year":"2014","unstructured":"Ghorbal M, Gorman M, Macpherson CR, Martins RM, Scherf A, Lopez-Rubio JJ (2014) Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Nat Biotechnol 32:819\u2013821. https:\/\/doi.org\/10.1038\/nbt.2925","journal-title":"Nat Biotechnol"},{"key":"4711_CR8","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1038\/nmeth.3063","volume":"11","author":"JC Wagner","year":"2014","unstructured":"Wagner JC, Platt RJ, Goldfless SJ, Zhang F, Niles JC (2014) Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum. Nat Methods 11:915\u2013918. https:\/\/doi.org\/10.1038\/nmeth.3063","journal-title":"Nat Methods"},{"key":"4711_CR9","doi-asserted-by":"publisher","first-page":"e0168362","DOI":"10.1371\/journal.pone.0168362","volume":"11","author":"CM Mogollon","year":"2016","unstructured":"Mogollon CM, van Pul FJ, Imai T, Ramesar J, Chevalley-Maurel S, de Roo GM, Veld SA, Kroeze H, Franke-Fayard BM, Janse CJ, Khan SM (2016) Rapid generation of marker-free P. falciparum fluorescent reporter lines using modified CRISPR\/Cas9 constructs and selection protocol. PLoS One 11:e0168362. https:\/\/doi.org\/10.1371\/journal.pone.0168362","journal-title":"PLoS One"},{"key":"4711_CR10","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1186\/s13071-016-1487-4","volume":"9","author":"J Lu","year":"2016","unstructured":"Lu J, Tong Y, Pan J, Yang Y, Liu Q, Tan X, Zhao S, Qin L, Chen X (2016) A redesigned CRISPR\/Cas9 system for marker-free genome editing in Plasmodium falciparum. Parasit Vectors 9:198. https:\/\/doi.org\/10.1186\/s13071-016-1487-4","journal-title":"Parasit Vectors"},{"key":"4711_CR11","doi-asserted-by":"publisher","first-page":"4078","DOI":"10.1182\/blood-2004-12-4666","volume":"105","author":"E Knuepfer","year":"2005","unstructured":"Knuepfer E, Rug M, Klonis N, Tilley L, Cowman AF (2005) Trafficking of the major virulence factor to the surface of transfected P. falciparum-infected erythrocytes. Blood 105:4078\u20134087. https:\/\/doi.org\/10.1182\/blood-2004-12-4666","journal-title":"Blood"},{"key":"4711_CR12","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1111\/j.1462-5822.2010.01481.x","volume":"12","author":"M Melcher","year":"2010","unstructured":"Melcher M, Muhle RA, Henrich PP, Kraemer SM, Avril M, Vigan-Womas I, Mercereau-Puijalon O, Smith JD, Fidock DA (2010) Identification of a role for the PfEMP1 semi-conserved head structure in protein trafficking to the surface of Plasmodium falciparum infected red blood cells. Cell Microbiol 12:1446\u20131462. https:\/\/doi.org\/10.1111\/j.1462-5822.2010.01481.x","journal-title":"Cell Microbiol"},{"key":"4711_CR13","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1016\/0035-9203(84)90221-9","volume":"78","author":"AR Dluzewski","year":"1984","unstructured":"Dluzewski AR, Ling IT, Rangachari K, Bates PA, Wilson RJ (1984) A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Trans R Soc Trop Med Hyg 78:622\u2013624","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"4711_CR14","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/S0091-679X(08)85002-9","volume":"85","author":"R Mealer","year":"2008","unstructured":"Mealer R, Butler H, Hughes T (2008) Functional fusion proteins by random transposon-based GFP insertion. Methods Cell Biol 85:23\u201344. https:\/\/doi.org\/10.1016\/S0091-679X(08)85002-9","journal-title":"Methods Cell Biol"},{"key":"4711_CR15","doi-asserted-by":"publisher","first-page":"3881","DOI":"10.1038\/s41598-017-03984-3","volume":"7","author":"E Knuepfer","year":"2017","unstructured":"Knuepfer E, Napiorkowska M, van Ooij C, Holder AA (2017) Generating conditional gene knockouts in Plasmodium - a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR\/Cas9. Sci Rep 7:3881. https:\/\/doi.org\/10.1038\/s41598-017-03984-3","journal-title":"Sci Rep"},{"key":"4711_CR16","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1038\/nmeth.2143","volume":"9","author":"J Straimer","year":"2012","unstructured":"Straimer J, Lee MC, Lee AH, Zeitler B, Williams AE, Pearl JR, Zhang L, Rebar EJ, Gregory PD, Llinas M, Urnov FD, Fidock DA (2012) Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases. Nat Methods 9:993\u2013998. https:\/\/doi.org\/10.1038\/nmeth.2143","journal-title":"Nat Methods"},{"key":"4711_CR17","doi-asserted-by":"publisher","first-page":"14574","DOI":"10.1038\/ncomms14574","volume":"8","author":"E Sonoiki","year":"2017","unstructured":"Sonoiki E, Ng CL, Lee MC, Guo D, Zhang YK, Zhou Y, Alley MR, Ahyong V, Sanz LM, Lafuente-Monasterio MJ, Dong C, Schupp PG, Gut J, Legac J, Cooper RA, Gamo FJ, DeRisi J, Freund YR, Fidock DA, Rosenthal PJ (2017) A potent antimalarial benzoxaborole targets a Plasmodium falciparum cleavage and polyadenylation specificity factor homologue. Nat Commun 8:14574. https:\/\/doi.org\/10.1038\/ncomms14574","journal-title":"Nat Commun"},{"key":"4711_CR18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033326","author":"S Iwanaga","year":"2012","unstructured":"Iwanaga S, Kato T, Kaneko I, Yuda M (2012) Centromere plasmid: a new genetic tool for the study of Plasmodium falciparum. PLoS ONE. https:\/\/doi.org\/10.1371\/journal.pone.0033326","journal-title":"PLoS ONE"},{"key":"4711_CR19","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1016\/j.chembiol.2010.07.009","volume":"17","author":"M Iwamoto","year":"2010","unstructured":"Iwamoto M, Bjorklund T, Lundberg C, Kirik D, Wandless TJ (2010) A general chemical method to regulate protein stability in the mammalian central nervous system. Chem Biol 17:981\u2013988. https:\/\/doi.org\/10.1016\/j.chembiol.2010.07.009","journal-title":"Chem Biol"},{"key":"4711_CR20","doi-asserted-by":"publisher","first-page":"4411","DOI":"10.1073\/pnas.1018449108","volume":"108","author":"V Muralidharan","year":"2011","unstructured":"Muralidharan V, Oksman A, Iwamoto M, Wandless TJ, Goldberg DE (2011) Asparagine repeat function in a Plasmodium falciparum protein assessed via a regulatable fluorescent affinity tag. Proc Natl Acad Sci USA 108:4411\u20134416. https:\/\/doi.org\/10.1073\/pnas.1018449108","journal-title":"Proc Natl Acad Sci USA"},{"key":"4711_CR21","doi-asserted-by":"publisher","first-page":"9286","DOI":"10.1073\/pnas.0602590103","volume":"103","author":"K El Bissati","year":"2006","unstructured":"El Bissati K, Zufferey R, Witola WH, Carter NS, Ullman B, Ben Mamoun C (2006) The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum. Proc Natl Acad Sci USA 103:9286\u20139291. https:\/\/doi.org\/10.1073\/pnas.0602590103","journal-title":"Proc Natl Acad Sci USA"},{"key":"4711_CR22","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1093\/bfgp\/elz012","volume":"18","author":"MCS Lee","year":"2019","unstructured":"Lee MCS, Lindner SE, Lopez-Rubio JJ, Llinas M (2019) Cutting back malaria: CRISPR\/Cas9 genome editing of Plasmodium. Brief Funct Genomics 18:281\u2013289. https:\/\/doi.org\/10.1093\/bfgp\/elz012","journal-title":"Brief Funct Genomics"},{"key":"4711_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.molbiopara.2016.12.010","volume":"212","author":"C Zhang","year":"2017","unstructured":"Zhang C, Gao H, Yang Z, Jiang Y, Li Z, Wang X, Xiao B, Su XZ, Cui H, Yuan J (2017) CRISPR\/Cas9 mediated sequential editing of genes critical for ookinete motility in Plasmodium yoelii. Mol Biochem Parasitol 212:1\u20138. https:\/\/doi.org\/10.1016\/j.molbiopara.2016.12.010","journal-title":"Mol Biochem Parasitol"},{"key":"4711_CR24","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1073\/pnas.0610353104","volume":"104","author":"DA Hill","year":"2007","unstructured":"Hill DA, Pillai AD, Nawaz F, Hayton K, Doan L, Lisk G, Desai SA (2007) A blasticidin S-resistant Plasmodium falciparum mutant with a defective plasmodial surface anion channel. Proc Natl Acad Sci U S A 104:1063\u20131068. https:\/\/doi.org\/10.1073\/pnas.0610353104","journal-title":"Proc Natl Acad Sci U S A"},{"key":"4711_CR25","doi-asserted-by":"publisher","first-page":"10931","DOI":"10.1073\/pnas.94.20.10931","volume":"94","author":"DA Fidock","year":"1997","unstructured":"Fidock DA, Wellems TE (1997) Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci U S A 94:10931\u201310936","journal-title":"Proc Natl Acad Sci U S A"},{"key":"4711_CR26","doi-asserted-by":"publisher","first-page":"8716","DOI":"10.1073\/pnas.96.15.8716","volume":"96","author":"CB Mamoun","year":"1999","unstructured":"Mamoun CB, Gluzman IY, Goyard S, Beverley SM, Goldberg DE (1999) A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum. Proc Natl Acad Sci USA 96:8716\u20138720","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["Molecular and Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11010-023-04711-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11010-023-04711-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11010-023-04711-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T16:13:19Z","timestamp":1744215199000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11010-023-04711-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,1]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["4711"],"URL":"https:\/\/doi.org\/10.1007\/s11010-023-04711-5","relation":{},"ISSN":["0300-8177","1573-4919"],"issn-type":[{"value":"0300-8177","type":"print"},{"value":"1573-4919","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,1]]},"assertion":[{"value":"19 December 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 March 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 April 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}