{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T21:08:00Z","timestamp":1793912880633,"version":"4.8.0"},"reference-count":112,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"crossref","award":["FONIS SA22I0041"],"award-info":[{"award-number":["FONIS SA22I0041"]}],"id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Cell Biochem"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1007\/s11010-024-05190-y","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T12:53:35Z","timestamp":1736168015000},"page":"3297-3314","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Advancing Parkinson\u2019s diagnosis: seed amplification assay for \u03b1-synuclein detection in minimally invasive samples"],"prefix":"10.1007","volume":"480","author":[{"given":"Elizabeth","family":"Carrazana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Montalb\u00e1n-Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Chana-Cuevas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8700-8520","authenticated-orcid":false,"given":"Natalia","family":"Salvadores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"issue":"3","key":"5190_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/nrdp.2017.13","volume":"23","author":"W Poewe","year":"2017","unstructured":"Poewe W, Seppi K, Tanner CM, Halliday GM, Brundin P, Volkmann J et al (2017) Parkinson disease. Nat Rev Dis Primers 23(3):1\u201321. https:\/\/doi.org\/10.1038\/nrdp.2017.13","journal-title":"Nat Rev Dis Primers"},{"key":"5190_CR2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00030-2","author":"S Tolosa","year":"2021","unstructured":"Tolosa S, Scholz W, Tolosa E, Garrido A, Scholz SW, Poewe W (2021) Challenges in the diagnosis of Parkinson\u2019s disease. Lancet Neurol. https:\/\/doi.org\/10.1016\/S1474-4422(21)00030-2","journal-title":"Lancet Neurol"},{"issue":"6","key":"5190_CR3","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1212\/WNL.0000000000002350","volume":"86","author":"G Rizzo","year":"2016","unstructured":"Rizzo G, Copetti M, Arcuti S, Martino D, Fontana A, Logroscino G (2016) Accuracy of clinical diagnosis of Parkinson disease. Neurology 86(6):566\u2013576","journal-title":"Neurology"},{"key":"5190_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(09)70238-8","author":"DW Dickson","year":"2009","unstructured":"Dickson DW, Braak H, Duda JE, Duyckaerts C, Gasser T, Halliday GM et al (2009) Neuropathological assessment of Parkinson\u2019s disease: refining the diagnostic criteria. Lancet Neurol. https:\/\/doi.org\/10.1016\/S1474-4422(09)70238-8","journal-title":"Lancet Neurol"},{"issue":"4","key":"5190_CR5","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1038\/s41591-024-02886-y","volume":"30","author":"G Pagano","year":"2024","unstructured":"Pagano G, Taylor KI, Anzures Cabrera J, Simuni T, Marek K, Postuma RB et al (2024) Prasinezumab slows motor progression in rapidly progressing early-stage Parkinson\u2019s disease. Nat Med 30(4):1096\u20131103","journal-title":"Nat Med"},{"issue":"2","key":"5190_CR6","doi-asserted-by":"publisher","first-page":"195","DOI":"10.3233\/JPD-181331","volume":"8","author":"TM Axelsen","year":"2018","unstructured":"Axelsen TM, Woldbye DPD (2018) Gene therapy for Parkinson\u2019s disease, an update. J Parkinsons Dis 8(2):195\u2013215. https:\/\/doi.org\/10.3233\/JPD-181331","journal-title":"J Parkinsons Dis"},{"issue":"8","key":"5190_CR7","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1136\/jnnp-2019-322338","volume":"91","author":"J Jankovic","year":"2020","unstructured":"Jankovic J, Tan EK (2020) Parkinson\u2019s disease: Etiopathogenesis and treatment. J Neurol Neurosurg Psychiatry 91(8):795\u2013808. https:\/\/doi.org\/10.1136\/jnnp-2019-322338","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"5190_CR8","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a009399","author":"L Stefanis","year":"2021","unstructured":"Stefanis L (2021) \u03b1-Synuclein in Parkinson\u2019s disease. Cold Spring Harb Perspect Med. https:\/\/doi.org\/10.1101\/cshperspect.a009399","journal-title":"Cold Spring Harb Perspect Med"},{"key":"5190_CR9","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.ijbiomac.2016.10.021","volume":"100","author":"D Ghosh","year":"2017","unstructured":"Ghosh D, Mehra S, Sahay S, Singh PK, Maji SK (2017) \u03b1-synuclein aggregation and its modulation. Int J Biol Macromol 100:37\u201354","journal-title":"Int J Biol Macromol"},{"key":"5190_CR10","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/s41531-022-00357-0","volume":"8","author":"P Magalh\u00e3es","year":"2022","unstructured":"Magalh\u00e3es P, Lashuel HA (2022) Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson\u2019s disease and other synucleinopathies. Npj Parkinson\u2019s Dis 8:93. https:\/\/doi.org\/10.1038\/s41531-022-00357-0","journal-title":"Npj Parkinson\u2019s Dis"},{"key":"5190_CR11","doi-asserted-by":"publisher","DOI":"10.3390\/biom13081263","author":"FJ Jim\u00e9nez-Jim\u00e9nez","year":"2023","unstructured":"Jim\u00e9nez-Jim\u00e9nez FJ, Alonso-Navarro H, Garc\u00eda-Mart\u00edn E, Santos-Garc\u00eda D, Mart\u00ednez-Valbuena I, Ag\u00fandez JAG (2023) Alpha-synuclein in peripheral tissues as a possible marker for neurological diseases and other medical conditions. Biomolecules. https:\/\/doi.org\/10.3390\/biom13081263","journal-title":"Biomolecules"},{"key":"5190_CR12","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.14809","author":"M Fayyad","year":"2019","unstructured":"Fayyad M, Salim S, Majbour N, Erskine D, Stoops E, Mollenhauer B et al (2019) Parkinson\u2019s disease biomarkers based on \u03b1-synuclein. J Neurochem. https:\/\/doi.org\/10.1111\/jnc.14809","journal-title":"J Neurochem"},{"key":"5190_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70213-7","author":"K Vekrellis","year":"2011","unstructured":"Vekrellis K, Xilouri M, Emmanouilidou E, Rideout HJ, Stefanis L, Stefanis L et al (2011) Pathological roles of \u03b1-synuclein in neurological disorders. Lancet Neurol. https:\/\/doi.org\/10.1016\/S1474-4422(11)70213-7","journal-title":"Lancet Neurol"},{"key":"5190_CR14","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05672-9","author":"P Calabresi","year":"2023","unstructured":"Calabresi P, Mechelli A, Natale G, Volpicelli-Daley L, Di Lazzaro G, Ghiglieri V (2023) Alpha-synuclein in Parkinson\u2019s disease and other synucleinopathies: from overt neurodegeneration back to early synaptic dysfunction. Cell Death Dis. https:\/\/doi.org\/10.1038\/s41419-023-05672-9","journal-title":"Cell Death Dis"},{"issue":"9","key":"5190_CR15","doi-asserted-by":"publisher","first-page":"1134","DOI":"10.1038\/s41593-022-01140-3","volume":"25","author":"ML Choi","year":"2022","unstructured":"Choi ML, Chappard A, Singh BP, Maclachlan C, Rodrigues M, Fedotova EI et al (2022) Pathological structural conversion of \u03b1-synuclein at the mitochondria induces neuronal toxicity. Nat Neurosci 25(9):1134\u20131148","journal-title":"Nat Neurosci"},{"key":"5190_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(23)00405-2","author":"T Simuni","year":"2024","unstructured":"Simuni T, Chahine LM, Poston K, Brumm M, Buracchio T, Campbell M et al (2024) A biological definition of neuronal \u03b1-synuclein disease: towards an integrated staging system for research. Lancet Neurol. https:\/\/doi.org\/10.1016\/S1474-4422(23)00405-2","journal-title":"Lancet Neurol"},{"issue":"16","key":"5190_CR17","doi-asserted-by":"publisher","first-page":"3350","DOI":"10.1016\/j.cell.2023.06.004","volume":"186","author":"J Xiang","year":"2023","unstructured":"Xiang J, Tao Y, Xia Y, Luo S, Zhao Q, Li B et al (2023) Development of an \u03b1-synuclein positron emission tomography tracer for imaging synucleinopathies. Cell 186(16):3350\u20133367. https:\/\/doi.org\/10.1016\/j.cell.2023.06.004","journal-title":"Cell"},{"issue":"9","key":"5190_CR18","doi-asserted-by":"publisher","first-page":"847","DOI":"10.3390\/ph14090847","volume":"14","author":"\u0160 Korat","year":"2021","unstructured":"Korat \u0160, Bidesi NSR, Bonanno F, Di Nanni A, Ho\u00e0ng ANN, Herfert K et al (2021) Alpha-Synuclein PET tracer development-an overview about current efforts. Pharmaceuticals 14(9):847. https:\/\/doi.org\/10.3390\/ph14090847","journal-title":"Pharmaceuticals"},{"key":"5190_CR19","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1038\/s41582-022-00618-9","volume":"18","author":"JY Lee","year":"2022","unstructured":"Lee JY, Martin-Bastida A, Murueta-Goyena A, Gabilondo I, Cuenca N, Piccini P et al (2022) Multimodal brain and retinal imaging of dopaminergic degeneration in Parkinson disease. Nat Rev Neurol 18:203\u2013220. https:\/\/doi.org\/10.1038\/s41582-022-00618-9","journal-title":"Nat Rev Neurol"},{"issue":"11","key":"5190_CR20","doi-asserted-by":"publisher","first-page":"e01808","DOI":"10.1002\/brb3.1808","volume":"10","author":"C Park","year":"2020","unstructured":"Park C, hyun, Lee PH, Lee SK, Chung SJ, Shin NY. (2020) The diagnostic potential of multimodal neuroimaging measures in Parkinson\u2019s disease and atypical parkinsonism. Brain Behav 10(11):e01808. https:\/\/doi.org\/10.1002\/brb3.1808","journal-title":"Brain Behav"},{"issue":"10","key":"5190_CR21","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.1001\/jamaneurol.2021.1312","volume":"78","author":"T Mitchell","year":"2021","unstructured":"Mitchell T, Leh\u00e9ricy S, Chiu SY, Strafella AP, Stoessl AJ, Vaillancourt DE (2021) Emerging neuroimaging biomarkers across disease stage in parkinson disease: a review. JAMA Neurol 78(10):1262\u20131272. https:\/\/doi.org\/10.1001\/jamaneurol.2021.1312","journal-title":"JAMA Neurol"},{"issue":"2","key":"5190_CR22","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1002\/mds","volume":"16","author":"B Holmberg","year":"2001","unstructured":"Holmberg B, Kallio M, Johnels B, Elam M (2001) Cardiovascular reflex testing contributes to clinical evaluation and differential diagnosis of Parkinsonian syndromes. Mov Disord 16(2):217\u2013225. https:\/\/doi.org\/10.1002\/mds","journal-title":"Mov Disord"},{"issue":"1","key":"5190_CR23","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1136\/jnnp.74.1.56","volume":"74","author":"DE Riley","year":"2003","unstructured":"Riley DE, Chelimsky TC (2003) Autonomic nervous system testing may not distinguish multiple system atrophy from Parkinson\u2019s disease. J Neurol Neurosurg Psychiatry 74(1):56\u201360. https:\/\/doi.org\/10.1136\/jnnp.74.1.56","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"5190_CR24","doi-asserted-by":"publisher","DOI":"10.1093\/sleep\/zsab172","author":"MG Miglis","year":"2021","unstructured":"Miglis MG, Zitser J, Schneider L, During E, Jaradeh S, Freeman R et al (2021) Cutaneous \u03b1-synuclein is correlated with autonomic impairment in isolated rapid eye movement sleep behavior disorder. Sleep. https:\/\/doi.org\/10.1093\/sleep\/zsab172","journal-title":"Sleep"},{"key":"5190_CR25","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2024.0792","author":"CH Gibbons","year":"2024","unstructured":"Gibbons CH, Levine T, Adler C, Bellaire B, Wang N, Stohl J et al (2024) Skin biopsy detection of phosphorylated \u03b1-synuclein in patients with synucleinopathies. JAMA. https:\/\/doi.org\/10.1001\/jama.2024.0792","journal-title":"JAMA"},{"issue":"5","key":"5190_CR26","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1007\/s00401-023-02557-1","volume":"145","author":"S Dutta","year":"2023","unstructured":"Dutta S, Hornung S, Taha HB, Bitan G (2023) Biomarkers for parkinsonian disorders in CNS-originating EVs: promise and challenges. Acta Neuropathol 145(5):515\u2013540. https:\/\/doi.org\/10.1007\/s00401-023-02557-1","journal-title":"Acta Neuropathol"},{"issue":"4","key":"5190_CR27","doi-asserted-by":"publisher","first-page":"1680","DOI":"10.1007\/s00415-023-12093-3","volume":"271","author":"HB Taha","year":"2024","unstructured":"Taha HB, Bogoniewski A (2024) Analysis of biomarkers in speculative CNS-enriched extracellular vesicles for parkinsonian disorders: a comprehensive systematic review and diagnostic meta-analysis. J Neurol 271(4):1680\u20131706. https:\/\/doi.org\/10.1007\/s00415-023-12093-3","journal-title":"J Neurol"},{"issue":"10","key":"5190_CR28","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1002\/acn3.338","volume":"3","author":"G Fairfoul","year":"2016","unstructured":"Fairfoul G, McGuire LI, Pal S, Ironside JW, Neumann J, Christie S et al (2016) Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies. Ann Clin Transl Neurol 3(10):812\u2013818. https:\/\/doi.org\/10.1002\/acn3.338","journal-title":"Ann Clin Transl Neurol"},{"issue":"1","key":"5190_CR29","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s40478-018-0508-2","volume":"6","author":"BR Groveman","year":"2018","unstructured":"Groveman BR, Orr\u00f9 CD, Hughson AG, Raymond LD, Zanusso G, Ghetti B et al (2018) Rapid and ultra-sensitive quantitation of disease-associated \u03b1-synuclein seeds in brain and cerebrospinal fluid by \u03b1Syn RT-QuIC. Acta Neuropathol Commun 6(1):7. https:\/\/doi.org\/10.1186\/s40478-018-0508-2","journal-title":"Acta Neuropathol Commun"},{"issue":"1","key":"5190_CR30","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/s00401-020-02160-8","volume":"140","author":"M Rossi","year":"2020","unstructured":"Rossi M, Candelise N, Baiardi S, Capellari S, Giannini G, Orr\u00f9 CD et al (2020) Ultrasensitive RT-QuIC assay with high sensitivity and specificity for Lewy body-associated synucleinopathies. Acta Neuropathol 140(1):49\u201362. https:\/\/doi.org\/10.1007\/s00401-020-02160-8","journal-title":"Acta Neuropathol"},{"key":"5190_CR31","doi-asserted-by":"publisher","DOI":"10.1002\/mds.28976","author":"M Luan","year":"2022","unstructured":"Luan M, Sun Y, Chen J, Jiang Y, Li F, Wei L et al (2022) Diagnostic value of salivary real-time quaking-induced conversion in parkinson\u2019s disease and multiple system atrophy. Mov Disord. https:\/\/doi.org\/10.1002\/mds.28976","journal-title":"Mov Disord"},{"key":"5190_CR32","doi-asserted-by":"publisher","DOI":"10.1002\/mds.29284","author":"A Garrido","year":"2023","unstructured":"Garrido A, Fairfoul G, Tolosa E, Marti MJ, Ezquerra M, Green AJE (2023) Brain and cerebrospinal fluid \u03b1-synuclein real-time quaking-induced conversion identifies lewy body pathology in LRRK2-PD. Mov Disord. https:\/\/doi.org\/10.1002\/mds.29284","journal-title":"Mov Disord"},{"key":"5190_CR33","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-021-01282-8","author":"MJ Russo","year":"2021","unstructured":"Russo MJ, Orru CD, Concha-Marambio L, Giaisi S, Groveman BR, Farris CM et al (2021) High diagnostic performance of independent alpha-synuclein seed amplification assays for detection of early Parkinson\u2019s disease. Acta Neuropathol Commun. https:\/\/doi.org\/10.1186\/s40478-021-01282-8","journal-title":"Acta Neuropathol Commun"},{"key":"5190_CR34","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(23)00109-6","author":"A Siderowf","year":"2023","unstructured":"Siderowf A, Concha-Marambio L, Lafontant DE, Farris CM, Ma Y, Urenia PA et al (2023) Assessment of heterogeneity among participants in the Parkinson\u2019s progression markers initiative cohort using \u03b1-synuclein seed amplification: a cross-sectional study. Lancet Neurol. https:\/\/doi.org\/10.1016\/S1474-4422(23)00109-6","journal-title":"Lancet Neurol"},{"key":"5190_CR35","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-021-01175-w","author":"C Bargar","year":"2021","unstructured":"Bargar C, Wang W, Gunzler SA, LeFevre A, Wang Z, Lerner AJ et al (2021) Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson\u2019s disease and dementia with Lewy bodies. Acta Neuropathol Commun. https:\/\/doi.org\/10.1186\/s40478-021-01175-w","journal-title":"Acta Neuropathol Commun"},{"key":"5190_CR36","doi-asserted-by":"publisher","DOI":"10.1002\/mds.29246","author":"G Vivacqua","year":"2023","unstructured":"Vivacqua G, Mason M, De Bartolo MI, W\u0119grzynowicz M, Cal\u00f2 L, Belvisi D et al (2023) Salivary \u03b1-synuclein RT-QuIC Correlates with disease severity in de novo parkinson\u2019s disease. Mov Disord. https:\/\/doi.org\/10.1002\/mds.29246","journal-title":"Mov Disord"},{"key":"5190_CR37","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02358-9","author":"A Okuzumi","year":"2023","unstructured":"Okuzumi A, Hatano T, Matsumoto G, Nojiri S, Ueno S, ichi, Imamichi-Tatano Y, et al (2023) Propagative \u03b1-synuclein seeds as serum biomarkers for synucleinopathies. Nat Med. https:\/\/doi.org\/10.1038\/s41591-023-02358-9","journal-title":"Nat Med"},{"issue":"6","key":"5190_CR38","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1001\/jama.2019.22360","volume":"323","author":"MJ Armstrong","year":"2020","unstructured":"Armstrong MJ, Okun MS (2020) Diagnosis and treatment of parkinson disease: a review. JAMA 323(6):548\u2013560. https:\/\/doi.org\/10.1001\/jama.2019.22360","journal-title":"JAMA"},{"key":"5190_CR39","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-019-0155-7","author":"A Elkouzi","year":"2019","unstructured":"Elkouzi A, Vedam-Mai V, Eisinger RS, Okun MS (2019) Emerging therapies in Parkinson disease \u2014 repurposed drugs and new approaches. Nat Rev Neurol. https:\/\/doi.org\/10.1038\/s41582-019-0155-7","journal-title":"Nat Rev Neurol"},{"key":"5190_CR40","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-024-01023-0","author":"LV Kalia","year":"2024","unstructured":"Kalia LV, AA, AN, et al (2024) Disease-modifying therapies for Parkinson disease: lessons from multiple sclerosis. Nat Rev Neurol. https:\/\/doi.org\/10.1038\/s41582-024-01023-0","journal-title":"Nat Rev Neurol"},{"key":"5190_CR41","doi-asserted-by":"publisher","DOI":"10.1007\/s10286-018-0581-4","author":"V Donadio","year":"2019","unstructured":"Donadio V (2019) Skin nerve \u03b1-synuclein deposits in Parkinson\u2019s disease and other synucleinopathies: a review. Clin Auton Res. https:\/\/doi.org\/10.1007\/s10286-018-0581-4","journal-title":"Clin Auton Res"},{"key":"5190_CR42","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.285","author":"I Rodr\u00edguez-Leyva","year":"2016","unstructured":"Rodr\u00edguez-Leyva I, Chi-Ahumada EG, Carrizales J, Rodr\u00edguez-Violante M, Vel\u00e1zquez-Osuna S, Medina-Mier V et al (2016) Parkinson disease and progressive supranuclear palsy: Protein expression in skin. Ann Clin Transl Neurol. https:\/\/doi.org\/10.1002\/acn3.285","journal-title":"Ann Clin Transl Neurol"},{"key":"5190_CR43","doi-asserted-by":"publisher","DOI":"10.1111\/ene.16041","author":"Y Zheng","year":"2023","unstructured":"Zheng Y, Li S, Yang C, Yu Z, Jiang Y, Feng T (2023) Comparison of biospecimens for \u03b1-synuclein seed amplification assays in Parkinson\u2019s disease: A systematic review and network meta-analysis. Eur J Neurol. https:\/\/doi.org\/10.1111\/ene.16041","journal-title":"Eur J Neurol"},{"key":"5190_CR44","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.104076","author":"Y Han","year":"2022","unstructured":"Han Y, Wu D, Wang Y, Xie J, Zhang Z (2022) Skin alpha-synuclein deposit patterns: a predictor of Parkinson\u2019s disease subtypes. EBioMedicine. https:\/\/doi.org\/10.1016\/j.ebiom.2022.104076","journal-title":"EBioMedicine"},{"key":"5190_CR45","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2023.120730","author":"H Peng","year":"2023","unstructured":"Peng H, Chen S, Wu S, Shi X, Ma J, Yang H et al (2023) Alpha-synuclein in skin as a high-quality biomarker for Parkinson\u2019s disease. J Neurol Sci. https:\/\/doi.org\/10.1016\/j.jns.2023.120730","journal-title":"J Neurol Sci"},{"key":"5190_CR46","doi-asserted-by":"publisher","DOI":"10.1038\/s41531-023-00519-8","author":"A Kuzkina","year":"2023","unstructured":"Kuzkina A, R\u00f6\u00dfle J, Seger A, Panzer C, Kohl A, Maltese V et al (2023) Combining skin and olfactory \u03b1-synuclein seed amplification assays (SAA)\u2014towards biomarker-driven phenotyping in synucleinopathies. NPJ Parkinsons Dis. https:\/\/doi.org\/10.1038\/s41531-023-00519-8","journal-title":"NPJ Parkinsons Dis"},{"key":"5190_CR47","doi-asserted-by":"publisher","DOI":"10.1212\/con.0000000000000814","author":"CH Gibbons","year":"2020","unstructured":"Gibbons CH, Wang N, Kim JY, Campagnolo M, Freeman R (2020) Skin biopsy in evaluation of autonomic disorders. Continuum. https:\/\/doi.org\/10.1212\/con.0000000000000814","journal-title":"Continuum"},{"key":"5190_CR48","doi-asserted-by":"publisher","DOI":"10.1369\/0022155420960250","author":"N Wang","year":"2020","unstructured":"Wang N, Garcia J, Freeman R, Gibbons CH (2020) Phosphorylated alpha-synuclein within cutaneous autonomic nerves of patients with parkinson\u2019s disease: the implications of sample thickness on results. J Histochem Cytochem. https:\/\/doi.org\/10.1369\/0022155420960250","journal-title":"J Histochem Cytochem"},{"key":"5190_CR49","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-024-01705-x","author":"J Li","year":"2024","unstructured":"Li J, Duan S, Yang J, Zheng H, Yuan Y, Tang M et al (2024) Detection of skin \u03b1-synuclein using RT-QuIC as a diagnostic biomarker for Parkinson\u2019s disease in the Chinese population. Eur J Med Res. https:\/\/doi.org\/10.1186\/s40001-024-01705-x","journal-title":"Eur J Med Res"},{"key":"5190_CR50","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000207147","author":"A Iranzo","year":"2023","unstructured":"Iranzo A, Mammana A, Mu\u00f1oz-Lopetegi A, Dellavalle S, May\u00e0 G, Rossi M et al (2023) Misfolded \u03b1-synuclein assessment in the skin and CSF by RT-QuIC in isolated REM sleep behavior disorder. Neurology. https:\/\/doi.org\/10.1212\/WNL.0000000000207147","journal-title":"Neurology"},{"issue":"6","key":"5190_CR51","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1002\/mds.29340","volume":"38","author":"A Kuzkina","year":"2023","unstructured":"Kuzkina A, Panzer C, Seger A, Schmitt D, R\u00f6\u00dfle J, Schreglmann SR et al (2023) Dermal real-time quaking-induced conversion is a sensitive marker to confirm isolated rapid eye movement sleep behavior disorder as an early \u03b1-synucleinopathy. Mov Disord 38(6):1077\u20131082","journal-title":"Mov Disord"},{"key":"5190_CR52","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2022.898516","author":"JL Chen","year":"2022","unstructured":"Chen JL, Huang Y, Xiao K, Gao LP, Chen C et al (2022) Application of \u03b1-Syn real-time quaking-induced conversion for brain and skin specimens of the chinese patients with parkinson\u2019s disease. Front Aging Neurosci. https:\/\/doi.org\/10.3389\/fnagi.2022.898516","journal-title":"Front Aging Neurosci"},{"issue":"1","key":"5190_CR53","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1038\/s41531-021-00242-2","volume":"7","author":"A Kuzkina","year":"2021","unstructured":"Kuzkina A, Bargar C, Schmitt D, R\u00f6\u00dfle J, Wang W, Schubert AL et al (2021) Diagnostic value of skin RT-QuIC in Parkinson\u2019s disease: a two-laboratory study. NPJ Parkinsons Dis 7(1):99","journal-title":"NPJ Parkinsons Dis"},{"key":"5190_CR54","doi-asserted-by":"publisher","DOI":"10.1002\/mds.28912","author":"I Martinez-Valbuena","year":"2022","unstructured":"Martinez-Valbuena I, Visanji NP, Olszewska DA, Sousa M, Bhakta P, Vasilevskaya A et al (2022) Combining skin \u03b1-synuclein real-time quaking-induced conversion and circulating neurofilament light chain to distinguish multiple system atrophy and parkinson\u2019s disease. Mov Disord. https:\/\/doi.org\/10.1002\/mds.28912","journal-title":"Mov Disord"},{"key":"5190_CR55","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000011935","author":"V Donadio","year":"2021","unstructured":"Donadio V, Wang Z, Incensi A, Rizzo G, Fileccia E, Vacchiano V et al (2021) In vivo diagnosis of synucleinopathies: a comparative study of skin biopsy and RT-QuIC. Neurology. https:\/\/doi.org\/10.1212\/WNL.0000000000011935","journal-title":"Neurology"},{"issue":"9","key":"5190_CR56","doi-asserted-by":"publisher","first-page":"2173","DOI":"10.1002\/mds.28651","volume":"36","author":"A Mammana","year":"2021","unstructured":"Mammana A, Baiardi S, Quadalti C, Rossi M, Donadio V, Capellari S et al (2021) RT-QuIC detection of pathological \u03b1-synuclein in skin punches of patients with lewy body disease. Mov Disord 36(9):2173\u20132177","journal-title":"Mov Disord"},{"issue":"1","key":"5190_CR57","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1001\/jamaneurol.2020.3311","volume":"78","author":"BK Wang","year":"2021","unstructured":"Wang BK, Donadio V, Siedlak S, Yuan J, Rezaee M et al (2021) Skin \u03b1-synuclein aggregation seeding activity as a novel biomarker for parkinson disease. JAMA Neurol 78(1):1\u201311","journal-title":"JAMA Neurol"},{"issue":"12","key":"5190_CR58","doi-asserted-by":"publisher","first-page":"2230","DOI":"10.1002\/mds.28242","volume":"35","author":"S Manne","year":"2020","unstructured":"Manne S, Kondru N, Jin H, Serrano GE, Anantharam V, Kanthasamy A et al (2020) Blinded RT-QuIC analysis of \u03b1-synuclein biomarker in skin tissue from parkinson\u2019s disease patients. Mov Disord 35(12):2230\u20132239","journal-title":"Mov Disord"},{"issue":"1","key":"5190_CR59","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1001\/jamaneurol.2020.3311","volume":"78","author":"Z Wang","year":"2021","unstructured":"Wang Z, Becker K, Donadio V, Siedlak S, Yuan J, Rezaee M et al (2021) Skin \u03b1-synuclein aggregation seeding activity as a novel biomarker for parkinson disease. JAMA Neurol 78(1):30\u201340","journal-title":"JAMA Neurol"},{"issue":"1","key":"5190_CR60","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1186\/1475-2875-8-279","volume":"8","author":"C Moneriz","year":"2009","unstructured":"Moneriz C, Mar\u00edn-Garc\u00eda P, Bautista JM, Diez A, Puyet A (2009) Haemoglobin interference and increased sensitivity of fluorimetric assays for quantification of low-parasitaemia Plasmodium infected erythrocytes. Malar J 8(1):279","journal-title":"Malar J"},{"key":"5190_CR61","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2023-1472","author":"A Mammana","year":"2024","unstructured":"Mammana A, Baiardi S, Rossi M, Quadalti C, Ticca A, Magliocchetti F et al (2024) Improving protocols for \u03b1-synuclein seed amplification assays: analysis of preanalytical and analytical variables and identification of candidate parameters for seed quantification. Clin Chem Lab Med. https:\/\/doi.org\/10.1515\/cclm-2023-1472","journal-title":"Clin Chem Lab Med"},{"issue":"5","key":"5190_CR62","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1002\/2211-5463.12844","volume":"10","author":"VC Ruf","year":"2020","unstructured":"Ruf VC, Shi S, Schmidt F, Weckbecker D, N\u00fcbling GS, K\u00f6del U et al (2020) Potential sources of interference with the highly sensitive detection and quantification of alpha-synuclein seeds by qRT-QuIC. FEBS Open Bio 10(5):883\u2013893","journal-title":"FEBS Open Bio"},{"key":"5190_CR63","doi-asserted-by":"publisher","DOI":"10.1002\/ar.10047","author":"JE Schwob","year":"2002","unstructured":"Schwob JE (2002) Neural regeneration and the peripheral olfactory system. Anat Rec. https:\/\/doi.org\/10.1002\/ar.10047","journal-title":"Anat Rec"},{"key":"5190_CR64","doi-asserted-by":"publisher","unstructured":"Tarakad A, Jankovic J. Chapter Seventeen. (2017) Anosmia and Ageusia in Parkinson\u2019s Disease. In: Nonmotor Parkinson\u2019s: The Hidden Face. https:\/\/doi.org\/10.1016\/bs.irn.2017.05.028.","DOI":"10.1016\/bs.irn.2017.05.028"},{"key":"5190_CR65","doi-asserted-by":"publisher","first-page":"569777","DOI":"10.3389\/fnins.2020.569777","volume":"14","author":"R He","year":"2020","unstructured":"He R, Zhao Y, He Y, Zhou Y, Yang J, Zhou X et al (2020) Olfactory dysfunction predicts disease progression in parkinson\u2019s disease: a longitudinal study. Front Neurosci 14:569777. https:\/\/doi.org\/10.3389\/fnins.2020.569777","journal-title":"Front Neurosci"},{"key":"5190_CR66","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2022.975619","author":"J Li","year":"2022","unstructured":"Li J, Luo H, Zheng H, Duan S, Zhao T, Yuan Y et al (2022) Clinical application of prion-like seeding in \u03b1-synucleinopathies: early and non-invasive diagnosis and therapeutic development. Front Mol Neurosci. https:\/\/doi.org\/10.3389\/fnmol.2022.975619","journal-title":"Front Mol Neurosci"},{"key":"5190_CR67","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-022-00311-3","author":"M Bongianni","year":"2022","unstructured":"Bongianni M, Catalan M, Perra D, Fontana E, Janes F, Bertolotti C et al (2022) Olfactory swab sampling optimization for \u03b1-synuclein aggregate detection in patients with Parkinson\u2019s disease. Transl Neurodegener. https:\/\/doi.org\/10.1186\/s40035-022-00311-3","journal-title":"Transl Neurodegener"},{"key":"5190_CR68","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-019-0164-x","author":"CMG De Luca","year":"2019","unstructured":"De Luca CMG, Elia AE, Portaleone SM, Cazzaniga FA, Rossi M, Bistaffa E et al (2019) Efficient RT-QuIC seeding activity for \u03b1-synuclein in olfactory mucosa samples of patients with Parkinson\u2019s disease and multiple system atrophy. Transl Neurodegener. https:\/\/doi.org\/10.1186\/s40035-019-0164-x","journal-title":"Transl Neurodegener"},{"issue":"1","key":"5190_CR69","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1186\/s13024-021-00491-y","volume":"16","author":"C Bargar","year":"2021","unstructured":"Bargar C, De Luca CMG, Devigili G, Elia AE, Cilia R, Portaleone SM et al (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener 16(1):82","journal-title":"Mol Neurodegener"},{"issue":"4","key":"5190_CR70","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1093\/brain\/awab005","volume":"144","author":"A Stefani","year":"2021","unstructured":"Stefani A, Iranzo A, Holzknecht E, Perra D, Bongianni M, Gaig C et al (2021) Alpha-synuclein seeds in olfactory mucosa of patients with isolated REM sleep behaviour disorder. Brain 144(4):1118\u20131126","journal-title":"Brain"},{"issue":"1","key":"5190_CR71","doi-asserted-by":"publisher","first-page":"87","DOI":"10.3390\/cells11010087","volume":"11","author":"CMG De Luca","year":"2022","unstructured":"De Luca CMG, Consonni A, Cazzaniga FA, Bistaffa E, Bufano G, Quitarrini G et al (2022) The alpha-synuclein RT-QuIC products generated by the olfactory mucosa of patients with parkinson\u2019s disease and multiple system atrophy induce inflammatory responses in SH-SY5Y cells. Cells 11(1):87. https:\/\/doi.org\/10.3390\/cells11010087","journal-title":"Cells"},{"issue":"2","key":"5190_CR72","doi-asserted-by":"publisher","first-page":"371","DOI":"10.3390\/diagnostics11020371","volume":"11","author":"P Pawlik","year":"2021","unstructured":"Pawlik P, B\u0142ochowiak K (2021) The role of salivary biomarkers in the early diagnosis of Alzheimer\u2019s disease and Parkinson\u2019s disease. Diagnostics 11(2):371","journal-title":"Diagnostics"},{"key":"5190_CR73","doi-asserted-by":"publisher","DOI":"10.3390\/cells13040340","author":"M Figura","year":"2020","unstructured":"Figura M, Friedman A (2020) Salivary proteome in Parkinson\u2019s disease. Eur J Neurol. https:\/\/doi.org\/10.3390\/cells13040340","journal-title":"Eur J Neurol"},{"issue":"3","key":"5190_CR74","first-page":"516","volume":"10","author":"S Polychronis","year":"2022","unstructured":"Polychronis S, Nasios G, Dardiotis E, Messinis L, Pagano G (2022) Pathophysiology and symptomatology of drooling in parkinson\u2019s disease. Healthcare (Switzerland) 10(3):516","journal-title":"Healthcare (Switzerland)"},{"issue":"2","key":"5190_CR75","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1002\/mds.27232","volume":"33","author":"JG Goldman","year":"2018","unstructured":"Goldman JG, Andrews H, Amara A, Naito A, Alcalay RN, Shaw LM et al (2018) Cerebrospinal fluid, plasma, and saliva in the BioFIND study: relationships among biomarkers and Parkinson\u2019s disease features. Mov Disord 33(2):282\u2013288","journal-title":"Mov Disord"},{"issue":"5","key":"5190_CR76","doi-asserted-by":"publisher","first-page":"661","DOI":"10.3390\/brainsci11050661","volume":"11","author":"M Figura","year":"2021","unstructured":"Figura M, Sitkiewicz E, \u015awiderska B, Milanowski \u0141, Szlufik S, Koziorowski D et al (2021) Proteomic profile of saliva in parkinson\u2019s disease patients: a proof of concept study. Brain Sci 11(5):661","journal-title":"Brain Sci"},{"issue":"10","key":"5190_CR77","first-page":"e29880","volume":"14","author":"P Muddaloor","year":"2022","unstructured":"Muddaloor P, Farinango M, Ansary A, Dakka A, Nazir Z, Shamim H et al (2022) Prospect of alpha-synuclein (A-Syn) isolation from saliva as a promising diagnostic biomarker alternative in parkinson\u2019s disease (PD): a systematic review. Cureus 14(10):e29880","journal-title":"Cureus"},{"key":"5190_CR78","doi-asserted-by":"publisher","DOI":"10.1101\/2024.06.29.24309703","author":"Z Wang","year":"2024","unstructured":"Wang Z, Gilliland T, Kim HJ, Gerasimenko M, Sajewski K, Camacho MV et al (2024) A minimally invasive biomarker for sensitive and accurate diagnosis of parkinson\u2019s disease. Acta Neuropathol Commun. https:\/\/doi.org\/10.1101\/2024.06.29.24309703","journal-title":"Acta Neuropathol Commun"},{"key":"5190_CR79","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1111\/jnc.14808","volume":"5","author":"P Alam","year":"2019","unstructured":"Alam P, Bousset L, Melki R, Otzen DE (2019) \u03b1-synuclein oligomers and fibrils: a spectrum of species, a spectrum of toxicities. J Neurochem 5:522\u2013534","journal-title":"J Neurochem"},{"issue":"5","key":"5190_CR80","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1002\/mds.28976","volume":"37","author":"M Luan","year":"2022","unstructured":"Luan M, Sun Y, Chen J, Jiang Y, Li F, Wei L et al (2022) Diagnostic value of salivary real-time quaking-induced conversion in parkinson\u2019s disease and multiple system atrophy. Mov Disord 37(5):1059\u20131063","journal-title":"Mov Disord"},{"key":"5190_CR81","doi-asserted-by":"publisher","DOI":"10.1515\/dx-2018-0018","author":"G Lippi","year":"2019","unstructured":"Lippi G, Von Meyer A, Cadamuro J, Simundic AM (2019) Blood sample quality. Diagnosis. https:\/\/doi.org\/10.1515\/dx-2018-0018","journal-title":"Diagnosis"},{"key":"5190_CR82","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.01388","author":"CW Chang","year":"2010","unstructured":"Chang CW, Yang SY, Yang CC, Chang CW, Wu YR (2010) Plasma and serum alpha-synuclein as a biomarker of diagnosis in patients with parkinson\u2019s disease. Front Neurol. https:\/\/doi.org\/10.3389\/fneur.2019.01388","journal-title":"Front Neurol"},{"key":"5190_CR83","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(19)30024-9","author":"L Parnetti","year":"2019","unstructured":"Parnetti L, Gaetani L, Eusebi P, Paciotti S, Hansson O, El-Agnaf O et al (2019) CSF and blood biomarkers for parkinson\u2019s disease. Lancet Neurol. https:\/\/doi.org\/10.1016\/S1474-4422(19)30024-9","journal-title":"Lancet Neurol"},{"key":"5190_CR84","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-7-42","author":"KM Danzer","year":"2012","unstructured":"Danzer KM, Kranich LR, Ruf WP, Cagsal-Getkin O, Winslow AR, Zhu L et al (2012) Exosomal cell-to-cell transmission of alpha synuclein oligomers. Mol Neurodegener. https:\/\/doi.org\/10.1186\/1750-1326-7-42","journal-title":"Mol Neurodegener"},{"key":"5190_CR85","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-021-02332-0","author":"S Dutta","year":"2021","unstructured":"Dutta S, Hornung S, Kruayatidee A, Maina KN, del Rosario I, Paul KC et al (2021) \u03b1-Synuclein in blood exosomes immunoprecipitated using neuronal and oligodendroglial markers distinguishes parkinson\u2019s disease from multiple system atrophy. Acta Neuropathol. https:\/\/doi.org\/10.1007\/s00401-021-02332-0","journal-title":"Acta Neuropathol"},{"key":"5190_CR86","doi-asserted-by":"publisher","DOI":"10.1111\/ene.14208","author":"M Niu","year":"2020","unstructured":"Niu M, Li Y, Li G, Zhou L, Luo N, Yao M et al (2020) A longitudinal study on \u03b1-synuclein in plasma neuronal exosomes as a biomarker for Parkinson\u2019s disease development and progression. Eur J Neurol. https:\/\/doi.org\/10.1111\/ene.14208","journal-title":"Eur J Neurol"},{"issue":"2","key":"5190_CR87","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1159\/000112832","volume":"5","author":"R Barbour","year":"2008","unstructured":"Barbour R, Kling K, Anderson JP, Banducci K, Cole T, Diep L et al (2008) Red blood cells are the major source of alpha-synuclein in blood. Neurodegener Dis 5(2):55\u201359","journal-title":"Neurodegener Dis"},{"key":"5190_CR88","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-020-02439-5","author":"S Klatt","year":"2020","unstructured":"Klatt S, Roberts A, Lothian A, Masters CL, Cappai R, Fowler C et al (2020) Optimizing red blood cell protein extraction for biomarker quantitation with mass spectrometry. Anal Bioanal Chem. https:\/\/doi.org\/10.1007\/s00216-020-02439-5","journal-title":"Anal Bioanal Chem"},{"key":"5190_CR89","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2023.1173074","author":"C Tian","year":"2019","unstructured":"Tian C, Liu G, Gao L, Soltys D, Pan C, Stewart T et al (2019) Erythrocytic \u03b1-synuclein as a potential biomarker for parkinson\u2019s disease. Transl Neurodegener. https:\/\/doi.org\/10.3389\/fneur.2023.1173074","journal-title":"Transl Neurodegener"},{"key":"5190_CR90","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac370","author":"A Kluge","year":"2022","unstructured":"Kluge A, Bunk J, Schaeffer E, Drobny A, Xiang W, Knacke H et al (2022) Detection of neuron-derived pathological \u03b1-synuclein in blood. Brain. https:\/\/doi.org\/10.1093\/brain\/awac370","journal-title":"Brain"},{"key":"5190_CR91","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2024.112366","author":"J Huang","year":"2024","unstructured":"Huang J, Yuan X, Chen L, Hu B, Wang H, Wang Y et al (2024) Pathological \u03b1-synuclein detected by real-time quaking-induced conversion in synucleinopathies. Exp Gerontol. https:\/\/doi.org\/10.1016\/j.exger.2024.112366","journal-title":"Exp Gerontol"},{"issue":"4","key":"5190_CR92","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1038\/s41596-022-00787-3","volume":"18","author":"L Concha-Marambio","year":"2023","unstructured":"Concha-Marambio L, Pritzkow S, Shahnawaz M, Farris CM, Soto C (2023) Seed amplification assay for the detection of pathologic alpha-synuclein aggregates in cerebrospinal fluid. Nat Protoc 18(4):1179\u20131196","journal-title":"Nat Protoc"},{"key":"5190_CR93","doi-asserted-by":"publisher","DOI":"10.1039\/C8CC02204F","author":"M T\u00f6rnquist","year":"2018","unstructured":"T\u00f6rnquist M, Michaels TCT, Sanagavarapu K, Yang X, Meisl G, Cohen SIA et al (2018) Secondary nucleation in amyloid formation. Chem Commun. https:\/\/doi.org\/10.1039\/C8CC02204F","journal-title":"Chem Commun"},{"key":"5190_CR94","doi-asserted-by":"publisher","first-page":"1193458","DOI":"10.3389\/fmolb.2023.1193458","volume":"10","author":"C Pe\u00f1a-Bautista","year":"2023","unstructured":"Pe\u00f1a-Bautista C, Kumar R, Baquero M, Johansson J, Ch\u00e1fer-Peric\u00e1s C, Abelein A et al (2023) Misfolded alpha-synuclein detection by RT-QuIC in dementia with lewy bodies: a systematic review and meta-analysis. Front Mol Biosci 10:1193458","journal-title":"Front Mol Biosci"},{"key":"5190_CR95","doi-asserted-by":"publisher","DOI":"10.3390\/biom11060820","author":"I Poggiolini","year":"2021","unstructured":"Poggiolini I, Erskine D, Vaikath NN, Ponraj J, Mansour S, Morris CM et al (2021) Rt-quic using c-terminally truncated \u03b1-synuclein forms detects differences in seeding propensity of different brain regions from synucleinopathies. Biomolecules. https:\/\/doi.org\/10.3390\/biom11060820","journal-title":"Biomolecules"},{"key":"5190_CR96","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.02.032","author":"M Masuda","year":"2006","unstructured":"Masuda M, Dohmae N, Nonaka T, Oikawa T, Hisanaga SI, Goedert M et al (2006) Cysteine misincorporation in bacterially expressed human \u03b1-synuclein. FEBS Lett. https:\/\/doi.org\/10.1016\/j.febslet.2006.02.032","journal-title":"FEBS Lett"},{"issue":"11","key":"5190_CR97","doi-asserted-by":"publisher","first-page":"10128","DOI":"10.1074\/jbc.M307563200","volume":"279","author":"W Zhou","year":"2004","unstructured":"Zhou W, Freed CR (2004) Tyrosine-to-cysteine modification of human \u03b1-synuclein enhances protein aggregation and cellular toxicity. J Biol Chem 279(11):10128\u201310135","journal-title":"J Biol Chem"},{"issue":"3","key":"5190_CR98","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1016\/j.bbrc.2008.01.140","volume":"368","author":"HJ Koo","year":"2008","unstructured":"Koo HJ, Lee HJ, Im H (2008) Sequence determinants regulating fibrillation of human \u03b1-synuclein. Biochem Biophys Res Commun 368(3):772\u2013778","journal-title":"Biochem Biophys Res Commun"},{"issue":"9","key":"5190_CR99","doi-asserted-by":"publisher","first-page":"2486","DOI":"10.3390\/ijms19092486","volume":"19","author":"AI Sulatskaya","year":"2018","unstructured":"Sulatskaya AI, Rodina NP, Sulatsky MI, Povarova OI, Antifeeva IA, Kuznetsova IM et al (2018) Investigation of \u03b1-synuclein amyloid fibrils using the fluorescent probe thioflavin T. Int J Mol Sci 19(9):2486","journal-title":"Int J Mol Sci"},{"key":"5190_CR100","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.160696","author":"C Xue","year":"2017","unstructured":"Xue C, Lin TY, Chang D, Guo Z (2017) Thioflavin T as an amyloid dye: Fibril quantification, optimal concentration, and effect on aggregation. R Soc Open Sci. https:\/\/doi.org\/10.1098\/rsos.160696","journal-title":"R Soc Open Sci"},{"issue":"7","key":"5190_CR101","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1016\/j.bbapap.2010.04.001","volume":"1804","author":"M Biancalana","year":"2010","unstructured":"Biancalana M, Koide S (2010) Molecular mechanism of Thioflavin-T binding to amyloid fibrils. Biochimica et Biophysica Acta - Proteins and Proteomics 1804(7):1405\u20131412","journal-title":"Biochimica et Biophysica Acta - Proteins and Proteomics"},{"key":"5190_CR102","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2022.105966","author":"J Estaun-Panzano","year":"2023","unstructured":"Estaun-Panzano J, Arotcarena ML, Bezard E (2023) Monitoring \u03b1-synuclein aggregation. Neurobiol Dis. https:\/\/doi.org\/10.1016\/j.nbd.2022.105966","journal-title":"Neurobiol Dis"},{"key":"5190_CR103","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2014.04.072","author":"E Coelho-Cerqueira","year":"2014","unstructured":"Coelho-Cerqueira E, Pinheiro AS, Follmer C (2014) Pitfalls associated with the use of Thioflavin-T to monitor anti-fibrillogenic activity. Bioorg Med Chem Lett. https:\/\/doi.org\/10.1016\/j.bmcl.2014.04.072","journal-title":"Bioorg Med Chem Lett"},{"key":"5190_CR104","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.795617","author":"H Kumar","year":"2017","unstructured":"Kumar H, Singh J, Kumari P, Udgaonkar JB (2017) Modulation of the extent of structural heterogeneity in \u03b1-synuclein fibrils by the small molecule thioflavin T. J Biol Chem. https:\/\/doi.org\/10.1074\/jbc.M117.795617","journal-title":"J Biol Chem"},{"key":"5190_CR105","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2022.106760","author":"MA Saraiva","year":"2022","unstructured":"Saraiva MA, Flor\u00eancio MH (2022) Buffering capacity is determinant for restoring early \u03b1-synuclein aggregation. Biophys Chem. https:\/\/doi.org\/10.1016\/j.bpc.2022.106760","journal-title":"Biophys Chem"},{"key":"5190_CR106","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-022-00283-4","author":"I Martinez-Valbuena","year":"2022","unstructured":"Martinez-Valbuena I, Visanji NP, Kim A, Lau HHC, So RWL, Alshimemeri S et al (2022) Alpha-synuclein seeding shows a wide heterogeneity in multiple system atrophy. Transl Neurodegener. https:\/\/doi.org\/10.1186\/s40035-022-00283-4","journal-title":"Transl Neurodegener"},{"key":"5190_CR107","doi-asserted-by":"publisher","DOI":"10.1017\/qrd.2020.7","author":"R Gaspar","year":"2020","unstructured":"Gaspar R, Lund M, Sparr E, Linse S (2020) Anomalous salt dependence reveals an interplay of attractive and repulsive electrostatic interactions in \u03b1-synuclein fibril formation. QRB Discov. https:\/\/doi.org\/10.1017\/qrd.2020.7","journal-title":"QRB Discov"},{"key":"5190_CR108","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.0c01292","author":"R Ramis","year":"2020","unstructured":"Ramis R, Ortega-Castro J, Vilanova B, Adrover M, Frau J (2020) Unraveling the NaCl concentration effect on the first stages of \u03b1-synuclein aggregation. Biomacromol. https:\/\/doi.org\/10.1021\/acs.biomac.0c01292","journal-title":"Biomacromol"},{"key":"5190_CR109","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212509","author":"JA Loureiro","year":"2021","unstructured":"Loureiro JA, Andrade S, Goderis L, Gomez-gutierrez R, Soto C, Morales R et al (2021) (De)stabilization of alpha-synuclein fibrillary aggregation by charged and uncharged surfactants. Int J Mol Sci. https:\/\/doi.org\/10.3390\/ijms222212509","journal-title":"Int J Mol Sci"},{"key":"5190_CR110","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-023-11810-2","author":"JS Middleton","year":"2023","unstructured":"Middleton JS, Hovren HL, Kha N, Medina MJ, MacLeod KR, Concha-Marambio L et al (2023) Seed amplification assay results illustrate discrepancy in Parkinson\u2019s disease clinical diagnostic accuracy and error rates. J Neurol. https:\/\/doi.org\/10.1007\/s00415-023-11810-2","journal-title":"J Neurol"},{"key":"5190_CR111","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000200878","author":"G Bellomo","year":"2022","unstructured":"Bellomo G, De Luca CMG, Paoletti FP, Gaetani L, Moda F, Parnetti L (2022) \u03b1-synuclein seed amplification assays for diagnosing synucleinopathies: the way forward. Neurology. https:\/\/doi.org\/10.1212\/WNL.0000000000200878","journal-title":"Neurology"},{"key":"5190_CR112","doi-asserted-by":"publisher","DOI":"10.1093\/braincomms\/fcab045","author":"D Perra","year":"2021","unstructured":"Perra D, Bongianni M, Novi G, Janes F, Bessi V, Capaldi S et al (2021) Alpha-synuclein seeds in olfactory mucosa and cerebrospinal fluid of patients with dementia with Lewy bodies. Brain Commun. https:\/\/doi.org\/10.1093\/braincomms\/fcab045","journal-title":"Brain Commun"}],"container-title":["Molecular and Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11010-024-05190-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11010-024-05190-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11010-024-05190-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T01:18:22Z","timestamp":1747876702000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11010-024-05190-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,6]]},"references-count":112,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["5190"],"URL":"https:\/\/doi.org\/10.1007\/s11010-024-05190-y","relation":{},"ISSN":["0300-8177","1573-4919"],"issn-type":[{"value":"0300-8177","type":"print"},{"value":"1573-4919","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,6]]},"assertion":[{"value":"9 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 January 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The manuscript does not contain clinical studies or patient data.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}