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Cells cultured in normoglycaemic (5.5\u00a0mM) or hyperglycaemic (25\u00a0mM, 6 to 120\u00a0h) conditions were treated with therapeutic hypothermia (35\u00a0\u00b0C) in the absence or presence of a PKC-\u03b2 inhibitor (LY333531, 0.05\u00a0\u03bcM) or a Nox2 inhibitor (gp91ds-tat, 50\u00a0\u03bcM). BBB was established by co-culture of human brain microvascular endothelial cells (HBMECs) with astrocytes (HAs) and pericytes. BBB integrity and function were assessed via transendothelial electrical resistance (TEER) and paracellular flux of sodium fluorescein (NaF, 376\u00a0Da). Nox activity (lucigenin assay), superoxide anion production (cytochrome-<jats:italic>C<\/jats:italic> reduction assay), cellular proliferative capacity (wound scratch assay) and actin cytoskeletal formation (rhodamine-phalloidin staining) were assessed both in HBMECs and HAs using the specific methodologies indicated in brackets. Therapeutic hypothermia augmented the protective effects of PKC-\u03b2 or Nox2 inhibition on BBB integrity and function in experimental setting of hyperglycaemia, as evidenced by increases in TEER and concomitant decreases in paracellular flux of NaF. The combinatory approaches were more effective in repairing physical damage exerted on HBMEC and HA monolayers by wound scratch and in decreasing Nox activity and superoxide anion production compared to sole treatment regimen with either agent. Similarly, the combinatory approaches were more effective in suppressing actin stress fibre formation and maintaining normal cytoskeletal structure. Therapeutic hypothermia augments the cerebral barrier-restorative capacity of agents specifically targeting PKC-\u03b2 or Nox2 pathways.<\/jats:p>","DOI":"10.1007\/s11011-021-00810-8","type":"journal-article","created":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T07:04:02Z","timestamp":1629097442000},"page":"1817-1832","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Therapeutic hypothermia augments the restorative effects of PKC-\u03b2 and Nox2 inhibition on an in vitro model of human blood\u2013brain barrier"],"prefix":"10.1007","volume":"36","author":[{"given":"Rais Reskiawan A.","family":"Kadir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansour","family":"Alwjwaj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"McCarthy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulvi","family":"Bayraktutan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,16]]},"reference":[{"key":"810_CR1","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.4103\/1673-5374.269029","volume":"15","author":"R Abdulkadir","year":"2020","unstructured":"Abdulkadir R, Alwjwaj M, Othman O, Rakkar K, Bayraktutan U (2020) Outgrowth endothelial cells form a functional cerebral barrier and restore its integrity after damage. 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