{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T11:22:18Z","timestamp":1772450538995,"version":"3.50.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31371501"],"award-info":[{"award-number":["31371501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81301041"],"award-info":[{"award-number":["81301041"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the National Undergraduate Scientific and Technological Innovation Project","award":["cx15015"],"award-info":[{"award-number":["cx15015"]}]},{"name":"Shaanxi Province Science and Technology projects","award":["2012K16-03-05"],"award-info":[{"award-number":["2012K16-03-05"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2016,8]]},"DOI":"10.1007\/s11033-016-4015-x","type":"journal-article","created":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T15:50:59Z","timestamp":1465573859000},"page":"837-847","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Validation of reference genes for quantitative real-time PCR in valproic acid rat models of autism"],"prefix":"10.1007","volume":"43","author":[{"given":"Jinlong","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junrong","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoming","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingfang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,6,10]]},"reference":[{"key":"4015_CR1","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.ntt.2012.08.005","volume":"36","author":"T Chomiak","year":"2013","unstructured":"Chomiak T, Hu B (2013) Alterations of neocortical development and maturation in autism: insight from valproic acid exposure and animal models of autism. Neurotoxicol Teratol 36:57\u201366. doi: 10.1016\/j.ntt.2012.08.005","journal-title":"Neurotoxicol Teratol"},{"key":"4015_CR2","doi-asserted-by":"publisher","first-page":"88","DOI":"10.3389\/fnbeh.2013.00088","volume":"7","author":"MR Favre","year":"2013","unstructured":"Favre MR, Barkat TR, Lamendola D, Khazen G, Markram H, Markram K (2013) General developmental health in the VPA-rat model of autism. Front Behav Neurosci 7:88. doi: 10.3389\/fnbeh.2013.00088","journal-title":"Front Behav Neurosci"},{"issue":"8","key":"4015_CR3","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1016\/j.ijdevneu.2013.09.002","volume":"31","author":"OS Cohen","year":"2013","unstructured":"Cohen OS, Varlinskaya EI, Wilson CA, Glatt SJ, Mooney SM (2013) Acute prenatal exposure to a moderate dose of valproic acid increases social behavior and alters gene expression in rats. Int J Dev Neurosci 31(8):740\u2013750. doi: 10.1016\/j.ijdevneu.2013.09.002","journal-title":"Int J Dev Neurosci"},{"issue":"5","key":"4015_CR4","doi-asserted-by":"publisher","first-page":"1254","DOI":"10.1016\/j.neubiorev.2010.12.013","volume":"35","author":"D Dufour-Rainfray","year":"2011","unstructured":"Dufour-Rainfray D, Vourc\u2019h P, Tourlet S, Guilloteau D, Chalon S, Andres CR (2011) Fetal exposure to teratogens: evidence of genes involved in autism. Neurosci Biobehav Rev 35(5):1254\u20131265. doi: 10.1016\/j.neubiorev.2010.12.013","journal-title":"Neurosci Biobehav Rev"},{"key":"4015_CR5","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.neulet.2014.03.066","volume":"569","author":"E Kumamaru","year":"2014","unstructured":"Kumamaru E, Egashira Y, Takenaka R, Takamori S (2014) Valproic acid selectively suppresses the formation of inhibitory synapses in cultured cortical neurons. Neurosci Lett 569:142\u2013147. doi: 10.1016\/j.neulet.2014.03.066","journal-title":"Neurosci Lett"},{"issue":"16","key":"4015_CR6","doi-asserted-by":"publisher","first-page":"2090","DOI":"10.1007\/s00018-007-7079-x","volume":"64","author":"G Rosenberg","year":"2007","unstructured":"Rosenberg G (2007) The mechanisms of action of valproate in neuropsychiatric disorders: can we see the forest for the trees? Cell Mol Life Sci 64(16):2090\u20132103. doi: 10.1007\/s00018-007-7079-x","journal-title":"Cell Mol Life Sci"},{"issue":"2","key":"4015_CR7","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.biopsych.2010.05.023","volume":"69","author":"EM Tunbridge","year":"2011","unstructured":"Tunbridge EM, Eastwood SL, Harrison PJ (2011) Changed relative to what? Housekeeping genes and normalization strategies in human brain gene expression studies. Biol Psychiatry 69(2):173\u2013179. doi: 10.1016\/j.biopsych.2010.05.023","journal-title":"Biol Psychiatry"},{"key":"4015_CR8","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1186\/1471-2199-10-78","volume":"10","author":"B Lallemant","year":"2009","unstructured":"Lallemant B, Evrard A, Combescure C, Chapuis H, Chambon G, Raynal C, Reynaud C, Sabra O, Joubert D, Hollande F, Lallemant JG, Lumbroso S, Brouillet JP (2009) Reference gene selection for head and neck squamous cell carcinoma gene expression studies. BMC Mol Biol 10:78. doi: 10.1186\/1471-2199-10-78","journal-title":"BMC Mol Biol"},{"key":"4015_CR9","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.neuro.2013.08.009","volume":"39","author":"Y He","year":"2013","unstructured":"He Y, Yu S, Bae E, Shen H, Wang Y (2013) Methamphetamine alters reference gene expression in nigra and striatum of adult rat brain. Neurotoxicology 39:138\u2013145. doi: 10.1016\/j.neuro.2013.08.009","journal-title":"Neurotoxicology"},{"issue":"2","key":"4015_CR10","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/j.1399-6576.2006.01161.x","volume":"51","author":"YF Tian","year":"2007","unstructured":"Tian YF, Zhang PB, Xiao XL, Zhang JS, Zhao JJ, Kang QY, Chen XL, Qiu F, Liu Y (2007) The quantification of ADAMTS expression in an animal model of cerebral ischemia using real-time PCR. Acta Anaesthesiol Scand 51(2):158\u2013164","journal-title":"Acta Anaesthesiol Scand"},{"issue":"2","key":"4015_CR11","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1038\/ijo.2013.86","volume":"38","author":"B Li","year":"2014","unstructured":"Li B, Matter EK, Hoppert HT, Grayson BE, Seeley RJ, Sandoval DA (2014) Identification of optimal reference genes for RT-qPCR in the rat hypothalamus and intestine for the study of obesity. Int J Obes 38(2):192\u2013197. doi: 10.1038\/ijo.2013.86","journal-title":"Int J Obes"},{"issue":"10","key":"4015_CR12","doi-asserted-by":"publisher","first-page":"e109902","DOI":"10.1371\/journal.pone.0109902","volume":"9","author":"GS Julian","year":"2014","unstructured":"Julian GS, de Oliveira RW, Perry JC, Tufik S, Chagas JR (2014) Validation of housekeeping genes in the brains of rats submitted to chronic intermittent hypoxia, a sleep apnea model. PLoS One 9(10):e109902. doi: 10.1371\/journal.pone.0109902","journal-title":"PLoS One"},{"key":"4015_CR13","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.niox.2015.07.003","volume":"49","author":"SR Oliveira","year":"2015","unstructured":"Oliveira SR, Vieira HL, Duarte CB (2015) Effect of carbon monoxide on gene expression in cerebrocortical astrocytes: validation of reference genes for quantitative real-time PCR. Nitric Oxide 49:80\u201389. doi: 10.1016\/j.niox.2015.07.003","journal-title":"Nitric Oxide"},{"issue":"4","key":"4015_CR14","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1002\/syn.20496","volume":"62","author":"BE Bonefeld","year":"2008","unstructured":"Bonefeld BE, Elfving B, Wegener G (2008) Reference genes for normalization: a study of rat brain tissue. Synapse 62(4):302\u2013309. doi: 10.1002\/syn.20496","journal-title":"Synapse"},{"issue":"1","key":"4015_CR15","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.devbrainres.2005.01.010","volume":"156","author":"MD Al-Bader","year":"2005","unstructured":"Al-Bader MD, Al-Sarraf HA (2005) Housekeeping gene expression during fetal brain development in the rat-validation by semi-quantitative RT-PCR. Brain Res Dev Brain Res 156(1):38\u201345. doi: 10.1016\/j.devbrainres.2005.01.010","journal-title":"Brain Res Dev Brain Res"},{"key":"4015_CR16","volume-title":"The rat brain in stereotaxic coordinates","year":"2009","unstructured":"Paxinos G, Watson C (eds) (2009) The rat brain in stereotaxic coordinates. Elsevier Acadamic Press, Burlington"},{"issue":"7","key":"4015_CR17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/gb-2002-3-7-research0034","volume":"3","author":"J Vandesompele","year":"2002","unstructured":"Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3(7):1\u20132","journal-title":"Genome Biol"},{"issue":"6","key":"4015_CR18","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1023\/B:BILE.0000019559.84305.47","volume":"26","author":"MW Pfaffl","year":"2004","unstructured":"Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol Lett 26(6):509\u2013515","journal-title":"Biotechnol Lett"},{"issue":"3","key":"4015_CR19","doi-asserted-by":"publisher","first-page":"e0122515","DOI":"10.1371\/journal.pone.0122515","volume":"10","author":"W Spiegelaere De","year":"2015","unstructured":"De Spiegelaere W, Dern-Wieloch J, Weigel R, Schumacher V, Schorle H, Nettersheim D, Bergmann M, Brehm R, Kliesch S, Vandekerckhove L, Fink C (2015) Reference gene validation for RT-qPCR, a note on different available software packages. PLoS One 10(3):e0122515. doi: 10.1371\/journal.pone.0122515","journal-title":"PLoS One"},{"issue":"15","key":"4015_CR20","doi-asserted-by":"publisher","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","volume":"64","author":"CL Andersen","year":"2004","unstructured":"Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64(15):5245\u20135250. doi: 10.1158\/0008-5472.CAN-04-0496","journal-title":"Cancer Res"},{"key":"4015_CR21","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-012-9885-2","author":"F Xie","year":"2012","unstructured":"Xie F, Xiao P, Chen D, Xu L, Zhang B (2012) miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Mol Biol. doi: 10.1007\/s11103-012-9885-2","journal-title":"Plant Mol Biol"},{"issue":"4","key":"4015_CR22","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25(4):402\u2013408. doi: 10.1006\/meth.2001.1262","journal-title":"Methods"},{"issue":"3","key":"4015_CR23","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1007\/s12031-011-9569-4","volume":"45","author":"H Toh","year":"2011","unstructured":"Toh H, Chitramuthu BP, Bennett HP, Bateman A (2011) Structure, function, and mechanism of progranulin; the brain and beyond. J Mol Neurosci 45(3):538\u2013548. doi: 10.1007\/s12031-011-9569-4","journal-title":"J Mol Neurosci"},{"key":"4015_CR24","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/1742-2094-8-111","volume":"8","author":"LY Al-Ayadhi","year":"2011","unstructured":"Al-Ayadhi LY, Mostafa GA (2011) Low plasma progranulin levels in children with autism. J Neuroinflamm 8:111. doi: 10.1186\/1742-2094-8-111","journal-title":"J Neuroinflamm"},{"issue":"3","key":"4015_CR25","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1007\/s12031-011-9553-z","volume":"45","author":"L Muynck De","year":"2011","unstructured":"De Muynck L, Van Damme P (2011) Cellular effects of progranulin in health and disease. J Mol Neurosci 45(3):549\u2013560. doi: 10.1007\/s12031-011-9553-z","journal-title":"J Mol Neurosci"},{"issue":"4","key":"4015_CR26","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","volume":"55","author":"SA Bustin","year":"2009","unstructured":"Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55(4):611\u2013622. doi: 10.1373\/clinchem.2008.112797","journal-title":"Clin Chem"},{"issue":"1","key":"4015_CR27","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.jneumeth.2009.12.018","volume":"187","author":"K Nelissen","year":"2010","unstructured":"Nelissen K, Smeets K, Mulder M, Hendriks JJ, Ameloot M (2010) Selection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR. J Neurosci Methods 187(1):78\u201383. doi: 10.1016\/j.jneumeth.2009.12.018","journal-title":"J Neurosci Methods"},{"key":"4015_CR28","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/1471-2199-12-36","volume":"12","author":"M Serrano","year":"2011","unstructured":"Serrano M, Moreno-Sanchez N, Gonzalez C, Marcos-Carcavilla A, Van Poucke M, Calvo JH, Salces J, Cubero J, Carabano MJ (2011) Use of maximum likelihood-mixed models to select stable reference genes: a case of heat stress response in sheep. BMC Mol Biol 12:36. doi: 10.1186\/1471-2199-12-36","journal-title":"BMC Mol Biol"},{"key":"4015_CR29","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/1471-2199-11-39","volume":"11","author":"R Mehta","year":"2010","unstructured":"Mehta R, Birerdinc A, Hossain N, Afendy A, Chandhoke V, Younossi Z, Baranova A (2010) Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples. BMC Mol Biol 11:39. doi: 10.1186\/1471-2199-11-39","journal-title":"BMC Mol Biol"},{"issue":"7","key":"4015_CR30","doi-asserted-by":"publisher","first-page":"1409","DOI":"10.1007\/s11064-012-0724-2","volume":"37","author":"Y Zhang","year":"2012","unstructured":"Zhang Y, Sun Y, Wang F, Wang Z, Peng Y, Li R (2012) Downregulating the canonical Wnt\/beta-catenin signaling pathway attenuates the susceptibility to autism-like phenotypes by decreasing oxidative stress. Neurochem Res 37(7):1409\u20131419. doi: 10.1007\/s11064-012-0724-2","journal-title":"Neurochem Res"},{"issue":"11","key":"4015_CR31","doi-asserted-by":"publisher","first-page":"1947","DOI":"10.1002\/ar.21232","volume":"293","author":"Z Wang","year":"2010","unstructured":"Wang Z, Xu L, Zhu X, Cui W, Sun Y, Nishijo H, Peng Y, Li R (2010) Demethylation of specific Wnt\/beta-catenin pathway genes and its upregulation in rat brain induced by prenatal valproate exposure. Anat Rec 293(11):1947\u20131953. doi: 10.1002\/ar.21232","journal-title":"Anat Rec"},{"key":"4015_CR32","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.mcn.2014.01.007","volume":"59","author":"LE Almeida","year":"2014","unstructured":"Almeida LE, Roby CD, Krueger BK (2014) Increased BDNF expression in fetal brain in the valproic acid model of autism. Mol Cell Neurosci 59:57\u201362. doi: 10.1016\/j.mcn.2014.01.007","journal-title":"Mol Cell Neurosci"},{"key":"4015_CR33","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/2051-5960-1-16","volume":"1","author":"J Banzhaf-Strathmann","year":"2013","unstructured":"Banzhaf-Strathmann J, Claus R, Mucke O, Rentzsch K, van der Zee J, Engelborghs S, De Deyn PP, Cruts M, van Broeckhoven C, Plass C, Edbauer D (2013) Promoter DNA methylation regulates progranulin expression and is altered in FTLD. Acta Neuropathol Commun 1:16. doi: 10.1186\/2051-5960-1-16","journal-title":"Acta Neuropathol Commun"},{"issue":"6","key":"4015_CR34","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1007\/s10072-012-1151-5","volume":"34","author":"D Galimberti","year":"2013","unstructured":"Galimberti D, D\u2019Addario C, Dell\u2019osso B, Fenoglio C, Marcone A, Cerami C, Cappa SF, Palazzo MC, Arosio B, Mari D, Maccarrone M, Bresolin N, Altamura AC, Scarpini E (2013) Progranulin gene (GRN) promoter methylation is increased in patients with sporadic frontotemporal lobar degeneration. Neurol Sci 34(6):899\u2013903. doi: 10.1007\/s10072-012-1151-5","journal-title":"Neurol Sci"},{"issue":"4","key":"4015_CR35","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1254\/jphs.12025SC","volume":"118","author":"Y Hara","year":"2012","unstructured":"Hara Y, Maeda Y, Kataoka S, Ago Y, Takuma K, Matsuda T (2012) Effect of prenatal valproic acid exposure on cortical morphology in female mice. J Pharmacol Sci 118(4):543\u2013546","journal-title":"J Pharmacol Sci"},{"issue":"6","key":"4015_CR36","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1111\/jnc.12147","volume":"124","author":"KC Kim","year":"2013","unstructured":"Kim KC, Kim P, Go HS, Choi CS, Park JH, Kim HJ, Jeon SJ, Dela Pena IC, Han SH, Cheong JH, Ryu JH, Shin CY (2013) Male-specific alteration in excitatory post-synaptic development and social interaction in pre-natal valproic acid exposure model of autism spectrum disorder. J Neurochem 124(6):832\u2013843. doi: 10.1111\/jnc.12147","journal-title":"J Neurochem"},{"issue":"6","key":"4015_CR37","doi-asserted-by":"publisher","first-page":"728","DOI":"10.1016\/j.psyneuen.2008.02.011","volume":"33","author":"T Schneider","year":"2008","unstructured":"Schneider T, Roman A, Basta-Kaim A, Kubera M, Budziszewska B, Schneider K, Przewlocki R (2008) Gender-specific behavioral and immunological alterations in an animal model of autism induced by prenatal exposure to valproic acid. Psychoneuroendocrinology 33(6):728\u2013740. doi: 10.1016\/j.psyneuen.2008.02.011","journal-title":"Psychoneuroendocrinology"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-016-4015-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11033-016-4015-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-016-4015-x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,24]],"date-time":"2017-06-24T12:09:00Z","timestamp":1498306140000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11033-016-4015-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,10]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["4015"],"URL":"https:\/\/doi.org\/10.1007\/s11033-016-4015-x","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,10]]}}}