{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T07:32:17Z","timestamp":1740123137036,"version":"3.37.3"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2018,1,23]],"date-time":"2018-01-23T00:00:00Z","timestamp":1516665600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100006181","name":"fapesb","doi-asserted-by":"crossref","award":["PNE009\/2011"],"award-info":[{"award-number":["PNE009\/2011"]}],"id":[{"id":"10.13039\/501100006181","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1007\/s11033-018-4150-7","type":"journal-article","created":{"date-parts":[[2018,1,23]],"date-time":"2018-01-23T08:32:06Z","timestamp":1516696326000},"page":"175-183","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cloning, expression, purification and spectrophotometric analysis of lanosterol 14-alpha demethylase from Leishmania braziliensis (LbCYP51)"],"prefix":"10.1007","volume":"45","author":[{"given":"Humberto F.","family":"Freitas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ac\u00e1ssia Benjamim","family":"Leal Pires","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo S.","family":"Castilho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,1,23]]},"reference":[{"key":"4150_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-94-007-7305-9_1","volume":"74","author":"JC Rodrigues","year":"2014","unstructured":"Rodrigues JC, Godinho JL, de Souza W (2014) Biology of human pathogenic trypanosomatids: epidemiology, lifecycle and ultrastructure. Subcell Biochem 74:1\u201342. https:\/\/doi.org\/10.1007\/978-94-007-7305-9_1","journal-title":"Subcell Biochem"},{"key":"4150_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jim.2015.03.017","volume":"422","author":"K Jain","year":"2015","unstructured":"Jain K, Jain NK (2015) Vaccines for visceral leishmaniasis: a review. J Immunol Methods 422:1\u201312. https:\/\/doi.org\/10.1016\/j.jim.2015.03.017","journal-title":"J Immunol Methods"},{"issue":"5","key":"4150_CR3","doi-asserted-by":"publisher","first-page":"e102","DOI":"10.1371\/journal.pmed.0030102","volume":"3","author":"PJ Hotez","year":"2006","unstructured":"Hotez PJ, Molyneux DH, Fenwick A, Ottesen E, Ehrlich Sachs S, Sachs JD (2006) Incorporating a rapid-impact package for neglected tropical diseases with programs for HIV\/AIDS, tuberculosis, and malaria. PLoS Med 3(5):e102. https:\/\/doi.org\/10.1371\/journal.pmed.0030102","journal-title":"PLoS Med"},{"issue":"11","key":"4150_CR4","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.pt.2003.09.008","volume":"19","author":"SL Croft","year":"2003","unstructured":"Croft SL, Coombs GH (2003) Leishmaniasis\u2013current chemotherapy and recent advances in the search for novel drugs. Trends Parasitol 19(11):502\u2013508","journal-title":"Trends Parasitol"},{"issue":"5","key":"4150_CR5","doi-asserted-by":"publisher","first-page":"e35671","DOI":"10.1371\/journal.pone.0035671","volume":"7","author":"J Alvar","year":"2012","unstructured":"Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M, Team WHOLC. (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7(5):e35671. https:\/\/doi.org\/10.1371\/journal.pone.0035671","journal-title":"PLoS ONE"},{"issue":"1","key":"4150_CR6","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1517\/14656566.2013.755515","volume":"14","author":"S Sundar","year":"2013","unstructured":"Sundar S, Chakravarty J (2013) Leishmaniasis: an update of current pharmacotherapy. Expert Opin Pharmacother 14(1):53\u201363. https:\/\/doi.org\/10.1517\/14656566.2013.755515","journal-title":"Expert Opin Pharmacother"},{"issue":"6","key":"4150_CR7","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1039\/C4MD00011K","volume":"5","author":"JN Burrows","year":"2014","unstructured":"Burrows JN, Elliott RL, Kaneko T, Mowbray CE, Waterson D (2014) The role of modern drug discovery in the fight against neglected and tropical diseases. MedChemComm 5(6):688\u2013700. https:\/\/doi.org\/10.1039\/C4MD00011K","journal-title":"MedChemComm"},{"key":"4150_CR8","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1590\/S0365-05962007000200002","volume":"82","author":"EB Lima","year":"2007","unstructured":"Lima EB, Porto C, Motta JOCD, Sampaio RNR (2007) Tratamento da Leishmaniose Tegumentar Americana. An Brasileiros de Dermatol 82:111\u2013124","journal-title":"An Brasileiros de Dermatol"},{"key":"4150_CR9","doi-asserted-by":"publisher","first-page":"S87-112","DOI":"10.1017\/S0031182006001831","volume":"133","author":"L Kedzierski","year":"2006","unstructured":"Kedzierski L, Zhu Y, Handman E (2006) Leishmania vaccines: progress and problems. Parasitology 133:S87-112. https:\/\/doi.org\/10.1017\/S0031182006001831","journal-title":"Parasitology"},{"key":"4150_CR10","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/646932","author":"S Sundar","year":"2014","unstructured":"Sundar S, Singh A, Singh OP (2014) Strategies to overcome antileishmanial drugs unresponsiveness. J Trop Med. https:\/\/doi.org\/10.1155\/2014\/646932","journal-title":"J Trop Med"},{"key":"4150_CR11","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1016\/j.ejmech.2017.01.022","volume":"127","author":"AH Romero","year":"2017","unstructured":"Romero AH, Medina R, Alcala A, Garcia-Marchan Y, Nunez-Duran J, Leanez J, Mijoba A, Ciangherotti C, Serrano-Martin X, Lopez SE (2017) Design, synthesis, structure-activity relationship and mechanism of action studies of a series of 4-chloro-1-phthalazinyl hydrazones as a potent agent against Leishmania braziliensis. Eur J Med Chem 127:606\u2013620. https:\/\/doi.org\/10.1016\/j.ejmech.2017.01.022","journal-title":"Eur J Med Chem"},{"issue":"2","key":"4150_CR12","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.ijpddr.2017.04.003","volume":"7","author":"KJH Alzahrani","year":"2017","unstructured":"Alzahrani KJH, Ali JAM, Eze AA, Looi WL, Tagoe DNA, Creek DJ, Barrett MP, de Koning HP (2017) Functional and genetic evidence that nucleoside transport is highly conserved in Leishmania species: implications for pyrimidine-based chemotherapy. Int J Parasitol 7(2):206\u2013226. https:\/\/doi.org\/10.1016\/j.ijpddr.2017.04.003","journal-title":"Int J Parasitol"},{"key":"4150_CR13","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1051\/parasite\/2014014","volume":"21","author":"K Aoun","year":"2014","unstructured":"Aoun K, Bouratbine A (2014) Cutaneous leishmaniasis in North Africa: a review. Parasite 21:14. https:\/\/doi.org\/10.1051\/parasite\/2014014","journal-title":"Parasite"},{"issue":"30","key":"4150_CR14","doi-asserted-by":"publisher","first-page":"9091","DOI":"10.1021\/bi034663f","volume":"42","author":"GI Lepesheva","year":"2003","unstructured":"Lepesheva GI, Virus C, Waterman MR (2003) Conservation in the CYP51 family. Role of the B\u2019 helix\/BC loop and helices F and G in enzymatic function. Biochemistry 42(30):9091\u20139101. https:\/\/doi.org\/10.1021\/bi034663f","journal-title":"Biochemistry"},{"issue":"31","key":"4150_CR15","doi-asserted-by":"crossref","first-page":"18502","DOI":"10.1016\/S0021-9258(18)51495-2","volume":"264","author":"Y Aoyama","year":"1989","unstructured":"Aoyama Y, Yoshida Y, Sonoda Y, Sato Y (1989) Deformylation of 32-oxo-24,25-dihydrolanosterol by the purified cytochrome P-45014DM (lanosterol 14 alpha-demethylase) from yeast evidence confirming the intermediate step of lanosterol 14 alpha-demethylation. J Biol Chem 264(31):18502\u201318505","journal-title":"J Biol Chem"},{"issue":"10","key":"4150_CR16","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1016\/S0022-2275(20)38245-6","volume":"30","author":"RT Fischer","year":"1989","unstructured":"Fischer RT, Stam SH, Johnson PR, Ko SS, Magolda RL, Gaylor JL, Trzaskos JM (1989) Mechanistic studies of lanosterol 14 alpha-methyl demethylase: substrate requirements for the component reactions catalyzed by a single cytochrome P-450 isozyme. J Lipid Res 30(10):1621\u20131632","journal-title":"J Lipid Res"},{"issue":"21","key":"4150_CR17","doi-asserted-by":"crossref","first-page":"12445","DOI":"10.1074\/jbc.271.21.12445","volume":"271","author":"AZ Shyadehi","year":"1996","unstructured":"Shyadehi AZ, Lamb DC, Kelly SL, Kelly DE, Schunck WH, Wright JN, Corina D, Akhtar M (1996) The mechanism of the acyl-carbon bond cleavage reaction catalyzed by recombinant sterol 14 alpha-demethylase of Candida albicans (other names are: lanosterol 14 alpha-demethylase, P-45014DM, and CYP51). J Biol Chem 271(21):12445\u201312450","journal-title":"J Biol Chem"},{"issue":"30","key":"4150_CR18","doi-asserted-by":"publisher","first-page":"26838","DOI":"10.1074\/jbc.M111.237099","volume":"286","author":"TY Hargrove","year":"2011","unstructured":"Hargrove TY, Wawrzak Z, Liu J, Nes WD, Waterman MR, Lepesheva GI (2011) Substrate preferences and catalytic parameters determined by structural characteristics of sterol 14alpha-demethylase (CYP51) from Leishmania infantum. J Biol Chem 286(30):26838\u201326848. https:\/\/doi.org\/10.1074\/jbc.M111.237099","journal-title":"J Biol Chem"},{"issue":"3","key":"4150_CR19","doi-asserted-by":"publisher","first-page":"1773","DOI":"10.1074\/jbc.M109.067470","volume":"285","author":"GI Lepesheva","year":"2010","unstructured":"Lepesheva GI, Park HW, Hargrove TY, Vanhollebeke B, Wawrzak Z, Harp JM, Sundaramoorthy M, Nes WD, Pays E, Chaudhuri M, Villalta F, Waterman MR (2010) Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections. J Biol Chem 285(3):1773\u20131780. https:\/\/doi.org\/10.1074\/jbc.M109.067470","journal-title":"J Biol Chem"},{"issue":"12","key":"4150_CR20","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1007\/s11745-008-3225-y","volume":"43","author":"GI Lepesheva","year":"2008","unstructured":"Lepesheva GI, Hargrove TY, Kleshchenko Y, Nes WD, Villalta F, Waterman MR (2008) CYP51: a major drug target in the cytochrome P450 superfamily. Lipids 43(12):1117\u20131125. https:\/\/doi.org\/10.1007\/s11745-008-3225-y","journal-title":"Lipids"},{"issue":"16","key":"4150_CR21","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.2174\/156802611796575902","volume":"11","author":"GI Lepesheva","year":"2011","unstructured":"Lepesheva GI, Waterman MR (2011) Sterol 14alpha-demethylase (CYP51) as a therapeutic target for human trypanosomiasis and leishmaniasis. Curr Top Med Chem 11(16):2060\u20132071","journal-title":"Curr Top Med Chem"},{"key":"4150_CR22","doi-asserted-by":"publisher","unstructured":"Froger A, Hall JE (2007) Transformation of plasmid DNA into E. coli using the heat shock method. J Vis Exp (6):253. https:\/\/doi.org\/10.3791\/253","DOI":"10.3791\/253"},{"issue":"11","key":"4150_CR23","doi-asserted-by":"publisher","first-page":"2411","DOI":"10.1002\/pro.5560041120","volume":"4","author":"CN Pace","year":"1995","unstructured":"Pace CN, Vajdos F, Fee L, Grimsley G, Gray T (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci 4(11):2411\u20132423. https:\/\/doi.org\/10.1002\/pro.5560041120","journal-title":"Protein Sci"},{"issue":"33","key":"4150_CR24","doi-asserted-by":"publisher","first-page":"10789","DOI":"10.1021\/bi048967t","volume":"43","author":"GI Lepesheva","year":"2004","unstructured":"Lepesheva GI, Nes WD, Zhou W, Hill GC, Waterman MR (2004) CYP51 from Trypanosoma brucei is obtusifoliol-specific. Biochemistry 43(33):10789\u201310799. https:\/\/doi.org\/10.1021\/bi048967t","journal-title":"Biochemistry"},{"issue":"1","key":"4150_CR25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0167-4838(99)00156-9","volume":"1434","author":"GI Lepesheva","year":"1999","unstructured":"Lepesheva GI, Strushkevich NV, Usanov SA (1999) Conformational dynamics and molecular interaction reactions of recombinant cytochrome p450scc (CYP11A1) detected by fluorescence energy transfer. Biochim et Biophys Acta 1434(1):31\u201343","journal-title":"Biochim et Biophys Acta"},{"issue":"16","key":"4150_CR26","doi-asserted-by":"crossref","first-page":"8937","DOI":"10.1073\/pnas.96.16.8937","volume":"96","author":"A Bellamine","year":"1999","unstructured":"Bellamine A, Mangla AT, Nes WD, Waterman MR (1999) Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis. Proc Natl Acad Sci USA 96(16):8937\u20138942","journal-title":"Proc Natl Acad Sci USA"},{"issue":"13","key":"4150_CR27","doi-asserted-by":"crossref","first-page":"3688","DOI":"10.1093\/nar\/gkg526","volume":"31","author":"T Vincze","year":"2003","unstructured":"Vincze T, Posfai J, Roberts RJ (2003) NEBcutter: a program to cleave DNA with restriction enzymes. Nucl Acids Res 31(13):3688\u20133691","journal-title":"Nucl Acids Res"},{"issue":"1","key":"4150_CR28","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/protein\/12.1.3","volume":"12","author":"H Nielsen","year":"1999","unstructured":"Nielsen H, Brunak S, von Heijne G (1999) Machine learning approaches for the prediction of signal peptides and other protein sorting signals. Protein Eng 12(1):3\u20139","journal-title":"Protein Eng"},{"key":"4150_CR29","unstructured":"Sonnhammer EL, von Heijne G, Krogh A (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. Proceedings \/ International Conference on Intelligent Systems for Molecular Biology; ISMB International Conference on Intelligent Systems for Molecular Biology, vol 6, pp 175\u2013182"},{"issue":"4","key":"4150_CR30","doi-asserted-by":"publisher","first-page":"e651","DOI":"10.1371\/journal.pntd.0000651","volume":"4","author":"CK Chen","year":"2010","unstructured":"Chen CK, Leung SS, Guilbert C, Jacobson MP, McKerrow JH, Podust LM (2010) Structural characterization of CYP51 from Trypanosoma cruzi and Trypanosoma brucei bound to the antifungal drugs posaconazole and fluconazole. PLoS Negl Trop Dis 4(4):e651. https:\/\/doi.org\/10.1371\/journal.pntd.0000651","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"4150_CR31","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.jbiotec.2004.08.004","volume":"115","author":"HP Sorensen","year":"2005","unstructured":"Sorensen HP, Mortensen KK (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115(2):113\u2013128. https:\/\/doi.org\/10.1016\/j.jbiotec.2004.08.004","journal-title":"J Biotechnol"},{"key":"4150_CR32","doi-asserted-by":"publisher","first-page":"172","DOI":"10.3389\/fmicb.2014.00172","volume":"5","author":"GL Rosano","year":"2014","unstructured":"Rosano GL, Ceccarelli EA (2014) Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol 5:172. https:\/\/doi.org\/10.3389\/fmicb.2014.00172","journal-title":"Front Microbiol"},{"issue":"3","key":"4150_CR33","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.bbagen.2006.07.018","volume":"1770","author":"GI Lepesheva","year":"2007","unstructured":"Lepesheva GI, Waterman MR (2007) Sterol 14alpha-demethylase cytochrome P450 (CYP51), a P450 in all biological kingdoms. Biochim et Biophys Acta 1770(3):467\u2013477. https:\/\/doi.org\/10.1016\/j.bbagen.2006.07.018","journal-title":"Biochim et Biophys Acta"},{"issue":"8","key":"4150_CR34","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1128\/AAC.32.8.1237","volume":"32","author":"JA Urbina","year":"1988","unstructured":"Urbina JA, Lazardi K, Aguirre T, Piras MM, Piras R (1988) Antiproliferative synergism of the allylamine SF 86\u2013327 and ketoconazole on epimastigotes and amastigotes of Trypanosoma (Schizotrypanum) cruzi. Antimicrob Agents Chemother 32(8):1237\u20131242","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"4150_CR35","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1128\/AAC.44.9.2498-2502.2000","volume":"44","author":"JA Urbina","year":"2000","unstructured":"Urbina JA, Lira R, Visbal G, Bartroli J (2000) In vitro antiproliferative effects and mechanism of action of the new triazole derivative UR-9825 against the protozoan parasite Trypanosoma (Schizotrypanum) cruzi. Antimicrob Agents Chemother 44(9):2498\u20132502","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"4150_CR36","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1111\/j.1574-6968.2007.00929.x","volume":"277","author":"L Zhao","year":"2007","unstructured":"Zhao L, Liu D, Zhang Q, Zhang S, Wan J, Xiao W (2007) Expression and homology modeling of sterol 14alpha-demethylase from Penicillium digitatum. FEMS Microbiol Lett 277(1):37\u201343. https:\/\/doi.org\/10.1111\/j.1574-6968.2007.00929.x","journal-title":"FEMS Microbiol Lett"},{"issue":"2","key":"4150_CR37","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/j.bbrc.2013.07.057","volume":"438","author":"H Ichinose","year":"2013","unstructured":"Ichinose H, Wariishi H (2013) High-level heterologous expression of fungal cytochrome P450s in Escherichia coli. Biochem Biophys Res Commun 438(2):289\u2013294. https:\/\/doi.org\/10.1016\/j.bbrc.2013.07.057","journal-title":"Biochem Biophys Res Commun"},{"issue":"8","key":"4150_CR38","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1002\/j.1460-2075.1987.tb02530.x","volume":"6","author":"AC Looman","year":"1987","unstructured":"Looman AC, Bodlaender J, Comstock LJ, Eaton D, Jhurani P, de Boer HA, van Knippenberg PH (1987) Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli. EMBO J 6(8):2489\u20132492","journal-title":"EMBO J"},{"key":"4150_CR39","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0076-6879(96)72003-7","volume":"272","author":"HJ Barnes","year":"1996","unstructured":"Barnes HJ (1996) Maximizing expression of eukaryotic cytochrome P450s in Escherichia coli. Methods Enzymol 272:3\u201314","journal-title":"Methods Enzymol"},{"issue":"2","key":"4150_CR40","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/j.pep.2010.06.005","volume":"74","author":"L Bivona","year":"2010","unstructured":"Bivona L, Zou Z, Stutzman N, Sun PD (2010) Influence of the second amino acid on recombinant protein expression. Protein Expr Purif 74(2):248\u2013256. https:\/\/doi.org\/10.1016\/j.pep.2010.06.005","journal-title":"Protein Expr Purif"},{"issue":"6","key":"4150_CR41","doi-asserted-by":"publisher","first-page":"3577","DOI":"10.1074\/jbc.M510317200","volume":"281","author":"GI Lepesheva","year":"2006","unstructured":"Lepesheva GI, Zaitseva NG, Nes WD, Zhou W, Arase M, Liu J, Hill GC, Waterman MR (2006) CYP51 from Trypanosoma cruzi: a phyla-specific residue in the B\u2019 helix defines substrate preferences of sterol 14alpha-demethylase. J Biol Chem 281(6):3577\u20133585. https:\/\/doi.org\/10.1074\/jbc.M510317200","journal-title":"J Biol Chem"},{"issue":"4","key":"4150_CR42","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1073\/pnas.59.4.1259","volume":"59","author":"B M\u00fcller-Hill","year":"1968","unstructured":"M\u00fcller-Hill B, Crapo L, Gilbert W (1968) Mutants that make more lac repressor. Proc Natl Acad Sci USA 59(4):1259\u20131264","journal-title":"Proc Natl Acad Sci USA"},{"key":"4150_CR43","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1385\/1-59259-998-2:11","volume":"320","author":"JB Schenkman","year":"2006","unstructured":"Schenkman JB, Jansson I (2006) Spectral analyses of cytochromes P450. Methods Mol Biol 320:11\u201318. https:\/\/doi.org\/10.1385\/1-59259-998-2:11","journal-title":"Methods Mol Biol"},{"issue":"33","key":"4150_CR44","doi-asserted-by":"publisher","first-page":"25582","DOI":"10.1074\/jbc.M110.133215","volume":"285","author":"GI Lepesheva","year":"2010","unstructured":"Lepesheva GI, Hargrove TY, Anderson S, Kleshchenko Y, Furtak V, Wawrzak Z, Villalta F, Waterman MR (2010) Structural insights into inhibition of sterol 14alpha-demethylase in the human pathogen Trypanosoma cruzi. J Biol Chem 285(33):25582\u201325590. https:\/\/doi.org\/10.1074\/jbc.M110.133215","journal-title":"J Biol Chem"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11033-018-4150-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-018-4150-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-018-4150-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,13]],"date-time":"2022-08-13T01:02:50Z","timestamp":1660352570000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11033-018-4150-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,23]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["4150"],"URL":"https:\/\/doi.org\/10.1007\/s11033-018-4150-7","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"type":"print","value":"0301-4851"},{"type":"electronic","value":"1573-4978"}],"subject":[],"published":{"date-parts":[[2018,1,23]]}}}