{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T20:44:35Z","timestamp":1778532275268,"version":"3.51.4"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2018,10]]},"DOI":"10.1007\/s11033-018-4244-2","type":"journal-article","created":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T15:24:55Z","timestamp":1531841095000},"page":"961-972","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Metabolic engineering of Pichia pastoris GS115 for enhanced pentose phosphate pathway (PPP) flux toward recombinant human interferon gamma (hIFN-\u03b3) production"],"prefix":"10.1007","volume":"45","author":[{"given":"Ashish A.","family":"Prabhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2035-811X","authenticated-orcid":false,"given":"Venkata Dasu","family":"Veeranki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,7,17]]},"reference":[{"issue":"6","key":"4244_CR1","doi-asserted-by":"publisher","first-page":"1094","DOI":"10.1016\/j.copbio.2013.02.024","volume":"24","author":"T Vogl","year":"2013","unstructured":"Vogl T, Hartner FS, Glieder A (2013) New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris. Curr Opin Biotechnol 24(6):1094\u20131101","journal-title":"Curr Opin Biotechnol"},{"key":"4244_CR2","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.copbio.2014.06.007","volume":"30","author":"MA Meehl","year":"2014","unstructured":"Meehl MA, Stadheim TA (2014) Biopharmaceutical discovery and production in yeast. Curr Opin Biotechnol 30:120\u2013127","journal-title":"Curr Opin Biotechnol"},{"key":"4244_CR3","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.bej.2010.07.017","volume":"64","author":"G Potvin","year":"2012","unstructured":"Potvin G, Ahmad A, Zhang Z (2012) Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: a review. Biochem Eng J 64:91\u2013105","journal-title":"Biochem Eng J"},{"issue":"12","key":"4244_CR4","doi-asserted-by":"publisher","first-page":"5301","DOI":"10.1007\/s00253-014-5732-5","volume":"98","author":"M Ahmad","year":"2014","unstructured":"Ahmad M, Hirz M, Pichler H, Schwab H (2014) Protein expression in Pichia pastoris. Appl Microbiol Biotechnol 98(12):5301\u20135317","journal-title":"Appl Microbiol Biotechnol"},{"issue":"6","key":"4244_CR5","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1016\/j.biotechadv.2015.05.008","volume":"33","author":"V Looser","year":"2015","unstructured":"Looser V et al (2015) Cultivation strategies to enhance productivity of Pichia pastoris: a review. Biotechnol Adv 33(6):1177\u20131193","journal-title":"Biotechnol Adv"},{"issue":"4","key":"4244_CR6","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1002\/yea.1208","volume":"22","author":"S Macauley-Patrick","year":"2005","unstructured":"Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast Chichester Engl 22(4):249\u2013270","journal-title":"Yeast Chichester Engl"},{"issue":"1","key":"4244_CR7","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1111\/j.1574-6976.2000.tb00532.x","volume":"24","author":"JL Cereghino","year":"2000","unstructured":"Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24(1):45\u201366","journal-title":"FEMS Microbiol Rev"},{"key":"4244_CR8","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.ces.2018.02.003","volume":"181","author":"AA Prabhu","year":"2018","unstructured":"Prabhu AA, Bharali B, Singh AK, Allaka M, Sukumar P, Veeranki VD (2018) Engineering folding mechanism through Hsp70 and Hsp40 chaperones for enhancing the production of recombinant human interferon gamma (rhIFN-\u03b3) in Pichia pastoris cell factory. Chem Eng Sci 181:58\u201367","journal-title":"Chem Eng Sci"},{"issue":"8","key":"4244_CR9","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1016\/j.tibtech.2016.02.010","volume":"34","author":"G Wu","year":"2016","unstructured":"Wu G, Yan Q, Jones JA, Tang YJ, Fong SS, Koffas MAG (2016) Metabolic burden: cornerstones in synthetic biology and metabolic engineering applications. Trends Biotechnol 34(8):652\u2013664","journal-title":"Trends Biotechnol"},{"issue":"15","key":"4244_CR10","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.2174\/1389201019666180329112827","volume":"18","author":"AA Prabhu","year":"2018","unstructured":"Prabhu AA, Boro B, Bharali B, Chakraborty S, Dasu VV (2018) Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichia pastoris. Curr Pharm Biotechnol 18(15):1200\u20131223","journal-title":"Curr Pharm Biotechnol"},{"issue":"2","key":"4244_CR11","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/0734-9750(95)00004-A","volume":"13","author":"BR Glick","year":"1995","unstructured":"Glick BR (1995) Metabolic load and heterologous gene expression. Biotechnol Adv 13(2):247\u2013261","journal-title":"Biotechnol Adv"},{"issue":"7","key":"4244_CR12","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1002\/bit.260350704","volume":"35","author":"WE Bentley","year":"1990","unstructured":"Bentley WE, Mirjalili N, Andersen DC, Davis RH, Kompala DS (1990) Plasmid-encoded protein: the principal factor in the \u2018metabolic burden\u2019 associated with recombinant bacteria. Biotechnol Bioeng 35(7):668\u2013681","journal-title":"Biotechnol Bioeng"},{"issue":"2","key":"4244_CR13","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1002\/bit.22836","volume":"107","author":"J Heyland","year":"2010","unstructured":"Heyland J, Fu J, Blank LM, Schmid A (2010) Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production. Biotechnol Bioeng 107(2):357\u2013368","journal-title":"Biotechnol Bioeng"},{"issue":"3","key":"4244_CR14","doi-asserted-by":"publisher","first-page":"1380","DOI":"10.1021\/pr8007623","volume":"8","author":"M Dragosits","year":"2009","unstructured":"Dragosits M et al (2009) The effect of temperature on the proteome of recombinant Pichia pastoris. J Proteome Res 8(3):1380\u20131392","journal-title":"J Proteome Res"},{"issue":"2","key":"4244_CR15","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1039\/B712395G","volume":"4","author":"HU Kim","year":"2008","unstructured":"Kim HU, Kim TY, Lee SY (2008) Metabolic flux analysis and metabolic engineering of microorganisms. Mol Biosyst 4(2):113\u2013120","journal-title":"Mol Biosyst"},{"issue":"10","key":"4244_CR16","doi-asserted-by":"crossref","first-page":"3724","DOI":"10.1128\/AEM.60.10.3724-3731.1994","volume":"60","author":"A Varma","year":"1994","unstructured":"Varma A, Palsson BO (1994) Stoichiometric flux balance models quantitatively predict growth and metabolic by-product secretion in wild-type Escherichia coli W3110. Appl Environ Microbiol 60(10):3724\u20133731","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"4244_CR17","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1093\/bib\/bbp011","volume":"10","author":"K Raman","year":"2009","unstructured":"Raman K, Chandra N (2009) Flux balance analysis of biological systems: applications and challenges. Brief Bioinform 10(4):435\u2013449","journal-title":"Brief Bioinform"},{"issue":"8","key":"4244_CR18","doi-asserted-by":"publisher","first-page":"3313","DOI":"10.1021\/pr2002885","volume":"10","author":"Y Toya","year":"2011","unstructured":"Toya Y, Kono N, Arakawa K, Tomita M (2011) Metabolic flux analysis and visualization. J Proteome Res 10(8):3313\u20133323","journal-title":"J Proteome Res"},{"issue":"3","key":"4244_CR19","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1007\/s10295-015-1585-x","volume":"42","author":"MR Antoniewicz","year":"2015","unstructured":"Antoniewicz MR (2015) Methods and advances in metabolic flux analysis: a mini-review. J Ind Microbiol Biotechnol 42(3):317\u2013325","journal-title":"J Ind Microbiol Biotechnol"},{"key":"4244_CR20","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.ymben.2014.05.011","volume":"24","author":"J Nocon","year":"2014","unstructured":"Nocon J et al (2014) Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production. Metab Eng 24:129\u2013138","journal-title":"Metab Eng"},{"key":"4244_CR21","doi-asserted-by":"publisher","first-page":"5955","DOI":"10.1007\/s00253-016-7363-5","volume":"100","author":"J Nocon","year":"2016","unstructured":"Nocon J et al (2016) Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris. Appl Microbiol Biotechnol 100:5955\u20135963","journal-title":"Appl Microbiol Biotechnol"},{"key":"4244_CR22","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1186\/s12864-015-1393-8","volume":"16","author":"R Prielhofer","year":"2015","unstructured":"Prielhofer R et al (2015) Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level. BMC Genom 16:167","journal-title":"BMC Genom"},{"issue":"6","key":"4244_CR23","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1016\/j.procbio.2016.02.007","volume":"51","author":"AA Prabhu","year":"2016","unstructured":"Prabhu AA, Veeranki VD, Dsilva SJ (2016) Improving the production of human interferon gamma (hIFN-\u03b3) in Pichia pastoris cell factory: an approach of cell level. Process Biochem 51(6):709\u2013718","journal-title":"Process Biochem"},{"issue":"4","key":"4244_CR24","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1007\/s11814-016-0358-1","volume":"34","author":"AA Prabhu","year":"2017","unstructured":"Prabhu AA, Mandal B, Dasu VV (2017) Medium optimization for high yield production of extracellular human interferon-\u03b3 from Pichia pastoris: a statistical optimization and neural network-based approach. Korean J Chem Eng 34(4):1109\u20131121","journal-title":"Korean J Chem Eng"},{"issue":"9","key":"4244_CR25","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1007\/s10295-017-1962-8","volume":"44","author":"P Wang","year":"2017","unstructured":"Wang P et al (2017) Accurate analysis of fusion expression of Pichia pastoris glycosylphosphatidylinositol-modified cell wall proteins. J Ind Microbiol Biotechnol 44(9):1355\u20131365.","journal-title":"J Ind Microbiol Biotechnol"},{"issue":"5","key":"4244_CR26","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.copbio.2003.08.001","volume":"14","author":"KJ Kauffman","year":"2003","unstructured":"Kauffman KJ, Prakash P, Edwards JS (2003) Advances in flux balance analysis. Curr Opin Biotechnol 14(5):491\u2013496","journal-title":"Curr Opin Biotechnol"},{"issue":"2","key":"4244_CR27","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1002\/bit.22543","volume":"105","author":"E \u00c7elik","year":"2010","unstructured":"\u00c7elik E, \u00c7al\u0131k P, Oliver SG (2010) Metabolic flux analysis for recombinant protein production by Pichia pastoris using dual carbon sources: effects of methanol feeding rate. Biotechnol Bioeng 105(2):317\u2013329","journal-title":"Biotechnol Bioeng"},{"issue":"2","key":"4244_CR28","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1101\/gr.234503","volume":"13","author":"J F\u00f6rster","year":"2003","unstructured":"F\u00f6rster J, Famili I, Fu P, Palsson B, Nielsen J (2003) Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network. Genome Res 13(2):244\u2013253","journal-title":"Genome Res"},{"issue":"6","key":"4244_CR29","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1038\/nbt.1544","volume":"27","author":"K Schutter De","year":"2009","unstructured":"De Schutter K et al (2009) Genome sequence of the recombinant protein production host Pichia pastoris. Nat Biotechnol 27(6):561\u2013566","journal-title":"Nat Biotechnol"},{"issue":"1","key":"4244_CR30","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1128\/EC.2.1.170-180.2003","volume":"2","author":"J Fiaux","year":"2003","unstructured":"Fiaux J, \u00c7akar ZP, Sonderegger M, W\u00fcthrich K, Szyperski T, Sauer U (2003) Metabolic-flux profiling of the yeasts Saccharomyces cerevisiae and Pichia stipitis. Eukaryot Cell 2(1):170\u2013180","journal-title":"Eukaryot Cell"},{"issue":"12","key":"4244_CR31","doi-asserted-by":"publisher","first-page":"2462","DOI":"10.1111\/j.1432-1033.2004.04176.x","volume":"271","author":"A Sol\u00e0","year":"2004","unstructured":"Sol\u00e0 A, Maaheimo H, Yl\u00f6nen K, Ferrer P, Szyperski T (2004) Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris. Eur J Biochem 271(12):2462\u20132470","journal-title":"Eur J Biochem"},{"key":"4244_CR32","doi-asserted-by":"publisher","first-page":"2512","DOI":"10.1016\/j.ijbiomac.2017.10.130","volume":"107","author":"AA Prabhu","year":"2017","unstructured":"Prabhu AA, Purkayastha A, Mandal B, Kumar JP, Mandal BB, Dasu VV (2017) A novel reverse micellar purification strategy for histidine tagged human interferon gamma (hIFN-\u03b3) protein from Pichia pastoris. Int J Biol Macromol 107:2512\u20132524","journal-title":"Int J Biol Macromol"},{"issue":"10","key":"4244_CR33","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1080\/10826068.2017.1350977","volume":"47","author":"AA Prabhu","year":"2017","unstructured":"Prabhu AA, Dasu VV (2017) Dual-substrate inhibition kinetic studies for recombinant human interferon gamma producing Pichia pastoris. Prep Biochem Biotechnol 47(10):953\u2013962","journal-title":"Prep Biochem Biotechnol"},{"issue":"3","key":"4244_CR34","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1080\/10826068.2016.1201685","volume":"47","author":"AP A","year":"2017","unstructured":"A AP, Chityala S, Garg Y, Dasu VV (2017) Reverse micellar extraction of papain with cationic detergent based system: an optimization approach. Prep Biochem Biotechnol 47(3):236\u2013244","journal-title":"Prep Biochem Biotechnol"},{"issue":"1","key":"4244_CR35","doi-asserted-by":"publisher","first-page":"1600105","DOI":"10.1002\/biot.201600105","volume":"12","author":"E Wells","year":"2017","unstructured":"Wells E, Robinson AS (2017) Cellular engineering for therapeutic protein production: product quality, host modification, and process improvement. Biotechnol J 12(1):1600105","journal-title":"Biotechnol J"},{"key":"4244_CR36","volume-title":"Biochemistry","author":"GL Zubay","year":"1988","unstructured":"Zubay GL, Atkinson DE (1988) Biochemistry. Macmillan, Collier Macmillan, New York"},{"key":"4244_CR37","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1038\/msb.2013.11","volume":"9","author":"GG Zampar","year":"2013","unstructured":"Zampar GG et al (2013) Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast. Mol Syst Biol 9:651","journal-title":"Mol Syst Biol"},{"issue":"18","key":"4244_CR38","doi-asserted-by":"publisher","first-page":"3379","DOI":"10.1091\/mbc.e11-02-0153","volume":"22","author":"LM Castelli","year":"2011","unstructured":"Castelli LM et al (2011) Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated. Mol Biol Cell 22(18):3379\u20133393","journal-title":"Mol Biol Cell"},{"issue":"4","key":"4244_CR39","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1002\/biot.201300334","volume":"9","author":"A Rebnegger","year":"2014","unstructured":"Rebnegger A et al (2014) In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response. Biotechnol J 9(4):511\u2013525","journal-title":"Biotechnol J"},{"key":"4244_CR40","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1385\/0-89603-421-6:107","volume":"103","author":"J Stratton","year":"1998","unstructured":"Stratton J, Chiruvolu V, Meagher M (1998) High cell-density fermentation. Methods Mol Biol Clifton NJ. 103:107\u2013120","journal-title":"Methods Mol Biol Clifton NJ"},{"issue":"4","key":"4244_CR41","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1007\/BF02942184","volume":"5","author":"W Zhang","year":"2000","unstructured":"Zhang W, Inan M, Meagher MM (2000) Fermentation strategies for recombinant protein expression in the methylotrophic yeast Pichia pastoris. Biotechnol Bioprocess Eng 5(4):275\u2013287","journal-title":"Biotechnol Bioprocess Eng"},{"issue":"9","key":"4244_CR42","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1002\/yea.1679","volume":"26","author":"E Celik","year":"2009","unstructured":"Celik E, Calik P, Oliver SG (2009) Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast Chichester Engl 26(9):473\u2013484","journal-title":"Yeast Chichester Engl"},{"issue":"2","key":"4244_CR43","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1002\/jctb.2292","volume":"85","author":"P \u00c7al\u0131k","year":"2010","unstructured":"\u00c7al\u0131k P et al (2010) Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation. J Chem Technol Biotechnol 85(2):226\u2013233","journal-title":"J Chem Technol Biotechnol"},{"issue":"1","key":"4244_CR44","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.bej.2011.03.007","volume":"55","author":"E Soyaslan","year":"2011","unstructured":"Soyaslan E, \u00c7al\u0131k P (2011) Enhanced recombinant human erythropoietin production by Pichia pastoris in methanol fed-batch\/sorbitol batch fermentation through pH optimization. Biochem Eng J 55(1):59\u201365","journal-title":"Biochem Eng J"},{"issue":"9","key":"4244_CR45","doi-asserted-by":"publisher","first-page":"1631","DOI":"10.1002\/jctb.4011","volume":"88","author":"P \u00c7al\u0131k","year":"2013","unstructured":"\u00c7al\u0131k P et al (2013) Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris. J Chem Technol Biotechnol 88(9):1631\u20131640","journal-title":"J Chem Technol Biotechnol"},{"issue":"4","key":"4244_CR46","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/S1389-1723(01)80236-X","volume":"92","author":"M Inan","year":"2001","unstructured":"Inan M, Meagher MM (2001) The effect of ethanol and acetate on protein expression in Pichia pastoris. J Biosci Bioeng 92(4):337\u2013341","journal-title":"J Biosci Bioeng"},{"key":"4244_CR47","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1099\/mic.0.29263-0","volume":"153","author":"A Sol\u00e0","year":"2007","unstructured":"Sol\u00e0 A, Jouhten P, Maaheimo H, S\u00e1nchez-Ferrando F, Szyperski T, Ferrer P (2007) Metabolic flux profiling of Pichia pastoris grown on glycerol\/methanol mixtures in chemostat cultures at low and high dilution rates. Microbiology 153:281\u201390","journal-title":"Microbiology."},{"key":"4244_CR48","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1002\/bit.22543","volume":"105","author":"Eda \u00c7elik","year":"2010","unstructured":"\u00c7elik Eda, \u00c7al\u0131k P\u0131nar, Oliver Stephen G (2010) Metabolic flux analysis for recombinant protein production by Pichia pastoris using dual carbon sources: effects of methanol feeding rate. Biotechnol Bioeng 105:317\u2013329","journal-title":"Biotechnol Bioeng"},{"issue":"11","key":"4244_CR49","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1002\/(SICI)1097-0061(199908)15:11<1087::AID-YEA436>3.0.CO;2-F","volume":"15","author":"MW Werten","year":"1999","unstructured":"Werten MW, van den Bosch TJ, Wind RD, Mooibroek H, de Wolf FA (1999) High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast Chichester Engl 15(11):1087\u20131096","journal-title":"Yeast Chichester Engl"},{"issue":"5","key":"4244_CR50","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1007\/s002530051659","volume":"53","author":"HA Kang","year":"2000","unstructured":"Kang HA et al (2000) Proteolytic stability of recombinant human serum albumin secreted in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 53(5):575\u2013582","journal-title":"Appl Microbiol Biotechnol"},{"issue":"1\u20132","key":"4244_CR51","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1007\/s11120-013-9943-x","volume":"118","author":"M Muthuraj","year":"2013","unstructured":"Muthuraj M, Palabhanvi B, Misra S, Kumar V, Sivalingavasu K, Das D (2013) Flux balance analysis of Chlorella sp. FC2 IITG under photoautotrophic and heterotrophic growth conditions. Photosynth Res 118(1\u20132):167\u2013179","journal-title":"Photosynth Res"},{"key":"4244_CR52","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.copbio.2016.04.017","volume":"42","author":"T Hirasawa","year":"2016","unstructured":"Hirasawa T, Shimizu H (2016) Recent advances in amino acid production by microbial cells. Curr Opin Biotechnol 42:133\u2013146","journal-title":"Curr Opin Biotechnol"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11033-018-4244-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-018-4244-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-018-4244-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T20:17:58Z","timestamp":1693772278000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11033-018-4244-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,17]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["4244"],"URL":"https:\/\/doi.org\/10.1007\/s11033-018-4244-2","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,17]]},"assertion":[{"value":"10 May 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 July 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 July 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}