{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:48:16Z","timestamp":1787960896300,"version":"build-2784847793"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T00:00:00Z","timestamp":1540425600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/07216-7"],"award-info":[{"award-number":["2016\/07216-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/11297-5"],"award-info":[{"award-number":["2017\/11297-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["138476\/2017-9"],"award-info":[{"award-number":["138476\/2017-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003046","name":"Empresa Brasileira de Pesquisa Agropecu\u00e1ria","doi-asserted-by":"publisher","award":["02.17.00.005.00.00"],"award-info":[{"award-number":["02.17.00.005.00.00"]}],"id":[{"id":"10.13039\/501100003046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2018,12]]},"DOI":"10.1007\/s11033-018-4436-9","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T19:37:38Z","timestamp":1540496258000},"page":"2671-2680","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Comparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring"],"prefix":"10.1007","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3692-3902","authenticated-orcid":false,"given":"Cintia Hiromi","family":"Okino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1700-0547","authenticated-orcid":false,"given":"Rodrigo","family":"Giglioti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3718-3157","authenticated-orcid":false,"given":"Pamella Cristini","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3181-1223","authenticated-orcid":false,"given":"Henrique Nunes","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7038-4607","authenticated-orcid":false,"given":"M\u00e1rcia Cristina","family":"de Sena Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,10,25]]},"reference":[{"issue":"Suppl","key":"4436_CR1","doi-asserted-by":"publisher","first-page":"S247","DOI":"10.1017\/S0031182004005190","volume":"129","author":"R Bock","year":"2004","unstructured":"Bock R, Jackson L, de Vos A, Jorgensen W (2004) Babesiosis of cattle. Parasitology 129(Suppl):S247\u2013S269","journal-title":"Parasitology"},{"key":"4436_CR2","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.vetpar.2005.03.007","volume":"130","author":"MCS Oliveira","year":"2005","unstructured":"Oliveira MCS, Oliveira-Sequeira TCG, Araujo JP Jr, Amarante AFT, Oliveira HN (2005) Babesia spp. infection in Boophilus microplus engorged female and eggs in S\u00e3o Paulo State, Brazil. Vet Parasitol 130:61\u201367. https:\/\/doi.org\/10.1016\/j.vetpar.2005.03.007","journal-title":"Vet Parasitol"},{"key":"4436_CR3","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.vetpar.2011.05.047","volume":"182","author":"MA Terkawi","year":"2011","unstructured":"Terkawi MA, Thekisoe OM, Katsande C, Latif AA, Mans BJ, Matthee O, Mkize N, Mabogoane N, Marais F, Yokoyama N, Xuan X, Igarashi I (2011) Serological survey of Babesia bovis and Babesia bigemina in cattle in South Africa. Vet Parasitol 182:337\u2013342. https:\/\/doi.org\/10.1016\/j.vetpar.2011.05.047","journal-title":"Vet Parasitol"},{"key":"4436_CR4","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1128\/JCM.30.6.1374-1379.1992","volume":"30","author":"Y Fahrimal","year":"1992","unstructured":"Fahrimal Y, Goff W, Jasmer DP (1992) Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA. J Clin Microbiol 30:1374\u20131379","journal-title":"J Clin Microbiol"},{"key":"4436_CR5","doi-asserted-by":"publisher","first-page":"75","DOI":"10.4236\/ojvm.2016.65009","volume":"6","author":"TA N\u00e9o","year":"2016","unstructured":"N\u00e9o TA, Giglioti R, Obreg\u00f3n D, Bilhassi TB, Oliveira HN, Machado RZ, An\u00edbal FF, Brito LG, Malag\u00f3 W Jr, Bressani FA, Oliveira MCS (2016) Detection of Babesia bovis and Babesia bigemina in water buffaloes (Bubalus bubalis) in endemic areas of S\u00e3o Paulo state, Brazil. Open J Vet Med 6:75\u201384","journal-title":"Open J Vet Med"},{"key":"4436_CR6","first-page":"39","volume":"49","author":"A Criado-Fornelio","year":"2007","unstructured":"Criado-Fornelio A (2007) A review of nucleic acid-based diagnostic tests for Babesia and Theileria, with emphasis on bovine piroplasms. Parassitologia 49:39\u201344","journal-title":"Parassitologia"},{"key":"4436_CR7","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1007\/s12639-016-0785-2","volume":"41","author":"AAT Al-Hosary","year":"2017","unstructured":"Al-Hosary AAT (2017) Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt. J Parasit Dis 41:243\u2013246. https:\/\/doi.org\/10.1007\/s12639-016-0785-2","journal-title":"J Parasit Dis"},{"key":"4436_CR8","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1111\/j.1574-6941.2008.00629.x","volume":"67","author":"CJ Smith","year":"2009","unstructured":"Smith CJ, Osborn AM (2009) Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol 67:6\u201320. https:\/\/doi.org\/10.1111\/j.1574-6941.2008.00629.x","journal-title":"FEMS Microbiol Ecol"},{"key":"4436_CR9","unstructured":"OIE (World Organisation for Animal Health) (2014) Bovine Babesiosis. In: OIE Manual of diagnostic tests and vaccines for terrestrial animals. http:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/2.04.02_BOVINE_BABESIOSIS.pdf . Accessed 8 Dec 2017"},{"key":"4436_CR10","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.vetpar.2007.03.031","volume":"147","author":"A Buling","year":"2007","unstructured":"Buling A, Criado-Fornelio A, Asenzo G, Benitez D, Barba-Carretero JC, Florin-Christensen M (2007) A quantitative PCR assay for the detection and quantification of B. bovis and B. bigemina. Vet Parasitol 147:16\u201325","journal-title":"Vet Parasitol"},{"key":"4436_CR11","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.vetpar.2009.03.040","volume":"162","author":"A Criado-Fornelio","year":"2009","unstructured":"Criado-Fornelio A, Buling A, Asenzo G, Benitez D, Florin-Christensen M, Gonzalez-Oliva A, Henriques G, Silva M, Alongi A, Agnone A, Torina A, Madruga CR (2009) Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids. Vet Parasitol 162:200\u2013206. https:\/\/doi.org\/10.1016\/j.vetpar.2009.03.040","journal-title":"Vet Parasitol"},{"key":"4436_CR12","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.vetpar.2010.10.035","volume":"176","author":"CA Ramos","year":"2011","unstructured":"Ramos CA, Ara\u00fajo FR, Souza II, Bacanelli G, Luiz HL, Russi LS, Oliveira RH, Soares CO, Rosinha GM, Alves LC (2011) Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis. Vet Parasitol 176:79\u201383. https:\/\/doi.org\/10.1016\/j.vetpar.2010.10.035","journal-title":"Vet Parasitol"},{"key":"4436_CR13","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.vetpar.2017.12.010","volume":"250","author":"R Giglioti","year":"2018","unstructured":"Giglioti R, de Oliveira HN, Bilhassi TB, Portilho AI, Okino CH, Marcondes CR, de Sena Oliveira MC (2018) Estimates of repeatability and correlations of hemoparasites infection levels for cattle reared in endemic areas for Rhipicephalus microplus. Vet Parasitol 250:78\u201384. https:\/\/doi.org\/10.1016\/j.vetpar.2017.12.010","journal-title":"Vet Parasitol"},{"key":"4436_CR14","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1016\/j.ttbdis.2016.02.011","volume":"7","author":"R Giglioti","year":"2016","unstructured":"Giglioti R, Oliveira HN, Santana CH, Ibelli AMG, N\u00e9o TA, Bilhassi TB, Rabelo MD, Machado RZ, Brito LG, Oliveira MCS (2016) Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of S\u00e3o Paulo state, Brazil. Ticks Tick Borne Dis 7:657\u2013662. https:\/\/doi.org\/10.1016\/j.ttbdis.2016.02.011","journal-title":"Ticks Tick Borne Dis"},{"key":"4436_CR15","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.ttbdis.2016.10.016","volume":"8","author":"Z Hailemariam","year":"2017","unstructured":"Hailemariam Z, Ahmed JS, Clausen PH, Nijhof AM (2017) A comparison of DNA extraction protocols from blood spotted on FTA cards for the detection of tick-borne pathogens by Reverse Line Blot hybridization. Ticks Tick Borne Dis 8:185\u2013189. https:\/\/doi.org\/10.1016\/j.ttbdis.2016.10.016","journal-title":"Ticks Tick Borne Dis"},{"key":"4436_CR16","unstructured":"OIE (World Organisation for Animal Health) (2014) Recommendations for validation of diagnostic tests. In: OIE Manual of diagnostic tests and vaccines for terrestrial animals. http:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/3.6.00_INTRODUCTION.pdf . Accessed 24 Jan 2018"},{"key":"4436_CR17","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1128\/CMR.13.3.451","volume":"13","author":"MJ Homer","year":"2000","unstructured":"Homer MJ, Aguilar-Delfin I, Telford SR 3rd, Krause PJ, Persing DH (2000) Babesiosis. Clin Microbiol Rev 13:451\u2013469","journal-title":"Clin Microbiol Rev"},{"key":"4436_CR18","volume-title":"Essentials of veterinary hematology","author":"NC Jain","year":"1993","unstructured":"Jain NC (1993) Essentials of veterinary hematology. Lea and Febiger, Philadelphia"},{"key":"4436_CR19","volume-title":"Duncan and Prasse\u2019s veterinary laboratory medicine: clinical pathology","author":"KW Prasse","year":"2003","unstructured":"Prasse KW, Latimer K, Mahaffey E (2003) Duncan and Prasse\u2019s veterinary laboratory medicine: clinical pathology, 4th\u00a0edn. Iowa State University Press, Ames (Iowa)","edition":"4"},{"key":"4436_CR20","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1128\/JCM.39.2.485-493.2001","volume":"39","author":"WA Al-Soud","year":"2001","unstructured":"Al-Soud WA, R\u00e5dstr\u00f6m P (2001) Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 39:485\u2013493","journal-title":"J Clin Microbiol"},{"key":"4436_CR21","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1186\/1756-0500-1-70","volume":"1","author":"JF Huggett","year":"2008","unstructured":"Huggett JF, Novak T, Garson JA, Green C, Morris-Jones SD, Miller RF, Zumla A (2008) Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon. BMC Res Notes 1:70. https:\/\/doi.org\/10.1186\/1756-0500-1-70","journal-title":"BMC Res Notes"},{"key":"4436_CR22","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1007\/s00216-009-3418-0","volume":"396","author":"M Buh Gasparic","year":"2010","unstructured":"Buh Gasparic M, Tengs T, La Paz JL, Holst-Jensen A, Pla M, Esteve T, Zel J, Gruden K (2010) Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection. Anal Bioanal Chem 396:2023\u20132029. https:\/\/doi.org\/10.1007\/s00216-009-3418-0","journal-title":"Anal Bioanal Chem"},{"key":"4436_CR23","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.jviromet.2005.10.004","volume":"132","author":"A Varga","year":"2006","unstructured":"Varga A, James D (2006) Real-time RT-PCR and SYBR Green I melting curve analysis for the identification of plum pox virus strains C, EA, and W: effect of amplicon size, melt rate, and dye translocation. J Virol Meth 132:146\u2013153","journal-title":"J Virol Meth"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11033-018-4436-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-018-4436-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-018-4436-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,14]],"date-time":"2020-11-14T02:17:18Z","timestamp":1605320238000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11033-018-4436-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,25]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["4436"],"URL":"https:\/\/doi.org\/10.1007\/s11033-018-4436-9","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,25]]},"assertion":[{"value":"1 August 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 October 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 October 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Animals used in this study were maintained in the experimental farm of Embrapa Pecu\u00e1ria Sudeste\u2014S\u00e3o Carlos, SP\u2014Brazil. All procedures have been approved by the Embrapa Pecu\u00e1ria Sudeste Ethical Committee for Animal Experimentation (CEUA\/CPPSE), following ethical principles and guidelines of animal experimentation adopted by the Brazilian College of Experimentation (process number PRT 02\/2017).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Research involving animals"}}]}}