{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T21:53:55Z","timestamp":1775771635423,"version":"3.50.1"},"reference-count":9,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T00:00:00Z","timestamp":1596326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T00:00:00Z","timestamp":1596326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"USDA AFRI","award":["2019-67015-29000"],"award-info":[{"award-number":["2019-67015-29000"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1007\/s11033-020-05701-2","type":"journal-article","created":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T08:02:41Z","timestamp":1596355361000},"page":"7277-7282","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A multiplex PCR genotyping assay to distinguish XX and XY tissues in sheep"],"prefix":"10.1007","volume":"47","author":[{"given":"River","family":"Evans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi J.","family":"Engelhorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quinton A.","family":"Winger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam J.","family":"Chicco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2433-5086","authenticated-orcid":false,"given":"Gerrit J.","family":"Bouma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,8,2]]},"reference":[{"issue":"1","key":"5701_CR1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.theriogenology.2007.09.021","volume":"69","author":"RV Anthony","year":"2008","unstructured":"Anthony RV, Barry JS (2008) The pregnant sheep as a model for human pregnancy. Theriogenology 69(1):55\u201367","journal-title":"Theriogenology"},{"key":"5701_CR2","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/1471-2156-6-16","volume":"6","author":"I Pfeiffer","year":"2005","unstructured":"Pfeiffer I, Brenig B (2005) X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis Aries) and European Red Deer (Cervus Elaphus). BMC Genet 6:16","journal-title":"BMC Genet"},{"key":"5701_CR3","unstructured":"Primer3Web version 4.1.0. http:\/\/bioinfo.ut.ee\/primer3-0.4.0\/. Accessed July, Sept 2019"},{"key":"5701_CR4","unstructured":"NCBI National Center for Biotechnology Information. https:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi. Accessed July, Sept 2019"},{"key":"5701_CR5","unstructured":"Genewiz. https:\/\/www.genewiz.com\/. Accessed Sept 2019"},{"key":"5701_CR6","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1186\/s13293-017-0154-6","volume":"8","author":"SJ Tunster","year":"2017","unstructured":"Tunster SJ (2017) Genetic sex determination of mice by simplex PCR. Biol Sex Differ 8:31","journal-title":"Biol Sex Differ"},{"issue":"3","key":"5701_CR7","doi-asserted-by":"publisher","first-page":"339","DOI":"10.2108\/zsj.20.339","volume":"20","author":"H Ito","year":"2017","unstructured":"Ito H et al (2003) Sex identification by alternative polymerase chain reaction methods in falcorniformes. Zool Sci 20(3):339\u2013344","journal-title":"Zool Sci"},{"issue":"5","key":"5701_CR8","doi-asserted-by":"publisher","first-page":"733","DOI":"10.5713\/ajas.2012.12003","volume":"25","author":"P Gokulakrishnan","year":"2012","unstructured":"Gokulakrishnan P (2012) Sex determination of cattle meat by polymerase chain reaction amplification of the DEAD box protein (DDX3X\/DDX3Y) gene. Asian-Aust J Anim Sci 25(5):733\u2013737","journal-title":"Asian-Aust J Anim Sci"},{"issue":"6","key":"5701_CR9","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1111\/j.1365-2052.1994.tb00533.x","volume":"25","author":"S Ennis","year":"1994","unstructured":"Ennis S, Gallagher TFA, PCR-Based (1994) Sex-determination assay in cattle based on the bovine amelogenin locus. Anim Genet 25(6):425\u2013427","journal-title":"Anim Genet"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-020-05701-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-020-05701-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-020-05701-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T23:36:46Z","timestamp":1627861006000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-020-05701-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,2]]},"references-count":9,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["5701"],"URL":"https:\/\/doi.org\/10.1007\/s11033-020-05701-2","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,2]]},"assertion":[{"value":"17 June 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 July 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 July 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 August 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All tissues were collected as part of a separate study, and all animal procedures were approved by Institutional Animal Care and Use Committee at Colorado State University, Fort Collins, Colorado, USA.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}