{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T08:53:05Z","timestamp":1776847985464,"version":"3.51.2"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100012021","name":"Iran University of Medical Sciences","doi-asserted-by":"crossref","award":["29461"],"award-info":[{"award-number":["29461"]}],"id":[{"id":"10.13039\/100012021","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2022,2]]},"DOI":"10.1007\/s11033-021-06833-9","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:04:12Z","timestamp":1642464252000},"page":"875-884","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The effect of Myo-Inositol supplement on molecular regulation of folliculogenesis, steroidogenesis, and assisted reproductive technique outcomes in patients with polycystic ovarian syndrome"],"prefix":"10.1007","volume":"49","author":[{"given":"Maryam","family":"Tabatabaie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadegh","family":"Amiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masoumeh","family":"Golestan Jahromi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azadeh Akbari","family":"Sene","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Zandieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Mehdizadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7489-7186","authenticated-orcid":false,"given":"Fatemehsadat","family":"Amjadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"issue":"11","key":"6833_CR1","doi-asserted-by":"publisher","first-page":"8763","DOI":"10.1007\/s11033-020-05924-3","volume":"47","author":"M Alikhani","year":"2020","unstructured":"Alikhani M, Amjadi F, Mirzaei M, Wu Y, Shekari F, Ashrafi M, Mehdizadeh M, McKay M, Taleahmad S, Aghajanpour S, Gupta V, Baharvand H, Aflatoonian R, Salekdeh GH (2020) Proteome analysis of endometrial tissue from patients with PCOS reveals proteins predicted to impact the disease. Mol Biol Rep 47(11):8763\u20138774","journal-title":"Mol Biol Rep"},{"issue":"4","key":"6833_CR2","first-page":"310","volume":"12","author":"F Hassani","year":"2019","unstructured":"Hassani F, Oryan S, Eftekhari-Yazdi P, Bazrgar M, Moini A, Nasiri N, Ghaheri A (2019) Association between the number of retrieved mature oocytes and insulin resistance or sensitivity in infertile women polycystic ovary syndrome. Int J Fertil Steril 12(4):310","journal-title":"Int J Fertil Steril"},{"issue":"8","key":"6833_CR3","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1002\/cbin.11367","volume":"44","author":"T Artimani","year":"2020","unstructured":"Artimani T, Najafi R (2020) APPL1 as an important regulator of insulin and adiponectin-signaling pathways in the PCOS: a narrative review. Cell Biol Int 44(8):1577\u20131587","journal-title":"Cell Biol Int"},{"key":"6833_CR4","volume-title":"Polycystic ovarian syndrome","author":"MM Orbetzova","year":"2020","unstructured":"Orbetzova MM (2020) Clinical impact of insulin resistance in women with polycystic ovary syndrome. In: Polycystic ovarian syndrome. IntechOpen, London"},{"key":"6833_CR5","doi-asserted-by":"crossref","unstructured":"Calcaterra V, Verduci E, Cena H, Magenes VC, Todisco CF, Tenuta E, Gregorio C, De Giuseppe R, Bosetti A, Di Profio E, Zuccotti G (2021) Polycystic ovary syndrome in insulin-resistant adolescents with obesity: the role of nutrition therapy and food supplements as a strategy to protect fertility. Nutrients 13(6):1848","DOI":"10.3390\/nu13061848"},{"issue":"5","key":"6833_CR6","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1210\/er.2015-1104","volume":"37","author":"RL Rosenfield","year":"2016","unstructured":"Rosenfield RL, Ehrmann DA (2016) The pathogenesis of polycystic ovary syndrome (PCOS): the hypothesis of PCOS as functional ovarian hyperandrogenism revisited. Endocr Rev 37(5):467\u2013520","journal-title":"Endocr Rev"},{"issue":"2","key":"6833_CR7","doi-asserted-by":"publisher","first-page":"131","DOI":"10.3109\/09513590.2014.964640","volume":"31","author":"Z Kamenov","year":"2015","unstructured":"Kamenov Z, Kolarov G, Gateva A, Carlomagno G, Genazzani AD (2015) Ovulation induction with myo-inositol alone and in combination with clomiphene citrate in polycystic ovarian syndrome patients with insulin resistance. Gynecol Endocrinol 31(2):131\u2013135","journal-title":"Gynecol Endocrinol"},{"issue":"1","key":"6833_CR8","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1186\/s12978-021-01207-7","volume":"18","author":"H Zhao","year":"2021","unstructured":"Zhao H, Xing C, Zhang J, He B (2021) Comparative efficacy of oral insulin sensitizers metformin, thiazolidinediones, inositol, and berberine in improving endocrine and metabolic profiles in women with PCOS: a network meta-analysis. Reprod Health 18(1):171","journal-title":"Reprod Health"},{"issue":"5","key":"6833_CR9","first-page":"e8079","volume":"12","author":"D Gupta","year":"2020","unstructured":"Gupta D, Khan S, Islam M, Malik BH, Rutkofsky IH (2020) Myo-Inositol\u2019s role in assisted reproductive technology: evidence for improving the quality of oocytes and embryos in patients with polycystic ovary syndrome. Cureus 12(5):e8079","journal-title":"Cureus"},{"issue":"23","key":"6833_CR10","doi-asserted-by":"publisher","first-page":"5566","DOI":"10.3390\/molecules25235566","volume":"25","author":"Z Kamenov","year":"2020","unstructured":"Kamenov Z, Gateva A (2020) Inositols in PCOS. Molecules 25(23):5566","journal-title":"Molecules"},{"issue":"16","key":"6833_CR11","doi-asserted-by":"publisher","first-page":"8433","DOI":"10.3390\/ijms22168433","volume":"22","author":"M Russo","year":"2021","unstructured":"Russo M, Forte G, Montanino Oliva M, Lagan\u00e0 AS, Unfer V (2021) Melatonin and Myo-Inositol: supporting reproduction from the oocyte to birth. Int J Mol Sci 22(16):8433","journal-title":"Int J Mol Sci"},{"issue":"23","key":"6833_CR12","first-page":"5491","volume":"21","author":"K Ravanos","year":"2017","unstructured":"Ravanos K, Monastra G, Pavlidou T, Goudakou M, Prapas N (2017) Can high levels of d-chiro-inositol in follicular fluid exert detrimental effects on blastocyst quality. Eur Rev Med Pharmacol Sci 21(23):5491\u20135498","journal-title":"Eur Rev Med Pharmacol Sci"},{"issue":"6","key":"6833_CR13","doi-asserted-by":"publisher","first-page":"504","DOI":"10.3390\/ph14060504","volume":"14","author":"R Gambioli","year":"2021","unstructured":"Gambioli R, Forte G, Buzzaccarini G, Unfer V, Lagan\u00e0 AS (2021) Myo-Inositol as a key supporter of fertility and physiological gestation. Pharmaceuticals 14(6):504","journal-title":"Pharmaceuticals"},{"issue":"19","key":"6833_CR14","doi-asserted-by":"publisher","first-page":"7157","DOI":"10.3390\/ijms21197157","volume":"21","author":"V Unfer","year":"2020","unstructured":"Unfer V, Dinicola S, Lagan\u00e0 AS, Bizzarri M (2020) Altered ovarian inositol ratios may account for pathological steroidogenesis in PCOS. Int J Mol Sci 21(19):7157","journal-title":"Int J Mol Sci"},{"issue":"2","key":"6833_CR15","doi-asserted-by":"publisher","first-page":"E237","DOI":"10.1152\/ajpendo.00162.2019","volume":"318","author":"H Cabrera-Cruz","year":"2020","unstructured":"Cabrera-Cruz H, Or\u00f3stica L, Plaza-Parrochia F, Torres-Pinto I, Romero C, Vega M (2020) The insulin-sensitizing mechanism of myo-inositol is associated with AMPK activation and GLUT-4 expression in human endometrial cells exposed to a PCOS environment. Am J Physiol Endocrinol Metab 318(2):E237\u2013E248","journal-title":"Am J Physiol Endocrinol Metab"},{"issue":"1","key":"6833_CR16","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1007\/s12020-015-0715-6","volume":"51","author":"PR Manna","year":"2016","unstructured":"Manna PR, Stetson CL, Slominski AT, Pruitt K (2016) Role of the steroidogenic acute regulatory protein in health and disease. Endocrine 51(1):7\u201321","journal-title":"Endocrine"},{"key":"6833_CR17","doi-asserted-by":"publisher","first-page":"692","DOI":"10.3389\/fendo.2019.00692","volume":"10","author":"Z Guo","year":"2019","unstructured":"Guo Z, Yu Q (2019) Role of mTOR signaling in female reproduction. Front Endocrinol (Lausanne) 10:692","journal-title":"Front Endocrinol (Lausanne)"},{"issue":"1","key":"6833_CR18","doi-asserted-by":"publisher","first-page":"107","DOI":"10.15407\/fz62.01.107","volume":"62","author":"E Shepel","year":"2016","unstructured":"Shepel E, Blashkiv T, Yanchii R (2016) Cumulus cell genes as potential biomarkers of oocyte and embryo developmental competence. Fiziol Zh 62(1):107\u2013113","journal-title":"Fiziol Zh"},{"issue":"5","key":"6833_CR19","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/S0015-0282(16)61034-8","volume":"52","author":"GA Hill","year":"1989","unstructured":"Hill GA, Freeman M, Bastias MC, Rogers BJ, Herbert CM III, Osteen KG, Wentz AC (1989) The influence of oocyte maturity and embryo quality on pregnancy rate in a program for in vitro fertilization-embryo transfer. Fertil Steril 52(5):801\u2013806","journal-title":"Fertil Steril"},{"issue":"4","key":"6833_CR20","first-page":"452","volume":"15","author":"V Unfer","year":"2011","unstructured":"Unfer V, Carlomagno G, Rizzo P, Raffone E, Roseff S (2011) Myo-inositol rather than d-chiro-inositol is able to improve oocyte quality in intracytoplasmic sperm injection cycles. A prospective, controlled, randomized trial. Rev Med Pharmacol Sci 15(4):452\u20137","journal-title":"Rev Med Pharmacol Sci"},{"issue":"6","key":"6833_CR21","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1007\/s00404-019-05111-1","volume":"299","author":"AA Sene","year":"2019","unstructured":"Sene AA, Tabatabaie A, Nikniaz H, Alizadeh A, Sheibani K, Alisaraie MM, Tabatabaie M, Ashrafi M, Amjadi F (2019) The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial. Arch Gynecol Obstet 299(6):1701\u20131707","journal-title":"Arch Gynecol Obstet"},{"key":"6833_CR22","doi-asserted-by":"publisher","DOI":"10.1515\/hmbci-2017-0067","author":"P-A Regidor","year":"2018","unstructured":"Regidor P-A, Schindler AE, Lesoine B, Druckman R (2018) Management of women with PCOS using myo-inositol and folic acid. New clinical data and review of the literature. Horm Mol Biol Clin Investig. https:\/\/doi.org\/10.1515\/hmbci-2017-0067","journal-title":"Horm Mol Biol Clin Investig"},{"issue":"10","key":"6833_CR23","first-page":"667","volume":"12","author":"R Davar","year":"2014","unstructured":"Davar R, Tabibnejad N, Kalantar SM, Sheikhha MH (2014) The luteinizing hormone beta-subunit exon 3 (Gly102Ser) gene mutation and ovarian responses to controlled ovarian hyperstimulation. Iran J Reprod Med 12(10):667","journal-title":"Iran J Reprod Med"},{"issue":"4","key":"6833_CR24","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/j.rbmo.2010.12.005","volume":"22","author":"M Gr\u00f8ndahl","year":"2011","unstructured":"Gr\u00f8ndahl M, Nielsen ME, Dal Canto M, Fadini R, Rasmussen I, Westergaard L, Kristensen SG, Yding Andersen C (2011) Anti-M\u00fcllerian hormone remains highly expressed in human cumulus cells during the final stages of folliculogenesis. Reprod Biomed Online 22(4):389\u2013398","journal-title":"Reprod Biomed Online"},{"issue":"2","key":"6833_CR25","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1093\/humrep\/dex357","volume":"33","author":"Owens L, Abbara A, Lerner A, O\u2019floinn S, Christopoulos G, Khanjani S, Islam R, Hardy K, Hanyaloglu AC, Lavery SA, Dhillo WS, Franks S","year":"2018","unstructured":"Owens L, Abbara A, Lerner A, O\u2019floinn S, Christopoulos G, Khanjani S, Islam R, Hardy K, Hanyaloglu AC, Lavery SA, Dhillo WS, Franks S (2018) The direct and indirect effects of kisspeptin-54 on granulosa lutein cell function. Hum Reprod 33(2):292\u2013302","journal-title":"Hum Reprod"},{"issue":"1","key":"6833_CR26","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1093\/biolre\/ioy102","volume":"99","author":"V Selvaraj","year":"2018","unstructured":"Selvaraj V, Stocco DM, Clark BJ (2018) Current knowledge on the acute regulation of steroidogenesis. Biol Reprod 99(1):13\u201326","journal-title":"Biol Reprod"},{"issue":"12","key":"6833_CR27","first-page":"771","volume":"13","author":"A-S Nazouri","year":"2015","unstructured":"Nazouri A-S, Khosravifar M, Akhlaghi A-A, Shiva M, Afsharian P (2015) No relationship between most polymorphisms of steroidogenic acute regulatory (StAR) gene with polycystic ovarian syndrome. Int J Reprod Med 13(12):771","journal-title":"Int J Reprod Med"},{"issue":"1","key":"6833_CR28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12884-021-03957-5","volume":"21","author":"N Emami","year":"2021","unstructured":"Emami N, Moini A, Yaghmaei P, Akbarinejad V, Shahhoseini M, Alizadeh A (2021) Differences in expression of genes related to steroidgenesis in abdominal subcutaneous adipose tissue of pregnant women with and without PCOS; a case control study. BMC Pregnancy Childbirth 21(1):1\u201310","journal-title":"BMC Pregnancy Childbirth"},{"issue":"1","key":"6833_CR29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13048-016-0301-4","volume":"8","author":"J Chen","year":"2015","unstructured":"Chen J, Shen S, Tan Y, Xia D, Xia Y, Cao Y, Wang W, Wu X, Wang H, Yi L, Gao Q, Wang Y (2015) The correlation of aromatase activity and obesity in women with or without polycystic ovary syndrome. J Ovarian Res 8(1):1\u20136","journal-title":"J Ovarian Res"},{"key":"6833_CR30","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8218650","author":"K Yu","year":"2019","unstructured":"Yu K, Wang R-X, Li M-H, Sun T-C, Zhou Y-W, Li Y-Y, Sun LH, Zhang BL, Lian ZX, Xue SG, Liu YX, Deng SL (2019) Melatonin reduces androgen production and upregulates heme oxygenase-1 expression in granulosa cells from PCOS patients with hypoestrogenia and hyperandrogenia. Oxid Med Cell Longev. https:\/\/doi.org\/10.1155\/2019\/8218650","journal-title":"Oxid Med Cell Longev"},{"issue":"12","key":"6833_CR31","doi-asserted-by":"publisher","first-page":"3523","DOI":"10.1093\/humrep\/des325","volume":"27","author":"D Haouzi","year":"2012","unstructured":"Haouzi D, Assou S, Monzo C, Vincens C, Dechaud H, Hamamah S (2012) Altered gene expression profile in cumulus cells of mature MII oocytes from patients with polycystic ovary syndrome. Hum Reprod 27(12):3523\u20133530","journal-title":"Hum Reprod"},{"issue":"4","key":"6833_CR32","doi-asserted-by":"publisher","first-page":"887","DOI":"10.5114\/aoms.2019.86060","volume":"15","author":"E Hosseini","year":"2019","unstructured":"Hosseini E, Shahhoseini M, Afsharian P, Karimian L, Ashrafi M, Mehraein F, Afatoonian R (2019) Role of epigenetic modifications in the aberrant CYP19A1 gene expression in polycystic ovary syndrome. Arch Med Sci 15(4):887","journal-title":"Arch Med Sci"},{"issue":"9","key":"6833_CR33","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1002\/mrd.22827","volume":"84","author":"HA LaVoie","year":"2017","unstructured":"LaVoie HA (2017) Transcriptional control of genes mediating ovarian follicular growth, differentiation, and steroidogenesis in pigs. Mol Reprod Dev 84(9):788\u2013801","journal-title":"Mol Reprod Dev"},{"issue":"11","key":"6833_CR34","doi-asserted-by":"publisher","first-page":"1483","DOI":"10.1042\/BCJ20160124","volume":"473","author":"J Dupont","year":"2016","unstructured":"Dupont J, Scaramuzzi RJ (2016) Insulin signalling and glucose transport in the ovary and ovarian function during the ovarian cycle. Biochem J 473(11):1483\u20131501","journal-title":"Biochem J"},{"issue":"23","key":"6833_CR35","doi-asserted-by":"publisher","first-page":"4257","DOI":"10.1128\/MCB.00734-14","volume":"34","author":"HS Abdou","year":"2014","unstructured":"Abdou HS, Bergeron F, Tremblay JJ (2014) A cell-autonomous molecular cascade initiated by AMP-activated protein kinase represses steroidogenesis. Mol Cell Biol 34(23):4257\u20134271","journal-title":"Mol Cell Biol"},{"key":"6833_CR36","doi-asserted-by":"publisher","first-page":"235","DOI":"10.3389\/fnins.2015.00235","volume":"9","author":"M Bertoldo","year":"2015","unstructured":"Bertoldo M, Faure M, Dupont J, Froment P (2015) AMPK: a master energy regulator for gonadal function. Front Neurosci 9:235","journal-title":"Front Neurosci"},{"key":"6833_CR37","doi-asserted-by":"publisher","first-page":"105722","DOI":"10.1016\/j.jsbmb.2020.105722","volume":"202","author":"P Wang","year":"2020","unstructured":"Wang P, Liu S, Zhu C, Duan Q, Jiang Y, Gao K, Bu Q, Cao B, An X (2020) MiR-29 regulates the function of goat granulosa cell by targeting PTX3 via the PI3K\/AKT\/mTOR and Erk1\/2 signaling pathways. J Steroid Biochem Mol Biol 202:105722","journal-title":"J Steroid Biochem Mol Biol"},{"key":"6833_CR38","doi-asserted-by":"publisher","first-page":"111965","DOI":"10.1016\/j.jep.2019.111965","volume":"250","author":"Z Qiu","year":"2020","unstructured":"Qiu Z, Dong J, Xue C, Li X, Liu K, Liu B, Cheng J, Huang F (2020) Liuwei Dihuang pills alleviate the polycystic ovary syndrome with improved insulin sensitivity through PI3K\/Akt signaling pathway. J Ethnopharmacol 250:111965","journal-title":"J Ethnopharmacol"},{"issue":"4","key":"6833_CR39","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1002\/mrd.20173","volume":"69","author":"C Vigneron","year":"2004","unstructured":"Vigneron C, Perreau C, Dupont J, Uzbekova S, Prigent C, Mermillod P (2004) Several signaling pathways are involved in the control of cattle oocyte maturation. Mol Reprod Dev 69(4):466\u2013474","journal-title":"Mol Reprod Dev"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-021-06833-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-021-06833-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-021-06833-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T17:31:35Z","timestamp":1644341495000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-021-06833-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,18]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["6833"],"URL":"https:\/\/doi.org\/10.1007\/s11033-021-06833-9","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,18]]},"assertion":[{"value":"16 June 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 October 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 January 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflicts of interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The research Committee of Iran University of medical Sciences reviewed and approved all aspects of this project with regard to ethical issues (IRCT2016122226668N2). The patients were informed consent.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"All authors consent to publication of this study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}