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However, despite the increasing number of cfDNA applications in liquid biopsies, we still lack a comprehensive understanding of the nature of cfDNA including optimal assessment. In the presented study, we continued testing and validation of common techniques for cfDNA extraction and quantification (qRT-PCR or droplet digital PCR) of nuclear- and mitochondrial cfDNA (ncfDNA and mtcfDNA) in blood, using a piglet model of perinatal asphyxia to determine potential temporal and quantitative changes at the levels of cfDNA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods and Results<\/jats:title>\n                <jats:p>Newborn piglets (n\u2009=\u200919) were either exposed to hypoxia (n\u2009=\u200911) or were part of the sham-operated control group (n\u2009=\u20098). Blood samples were collected at baseline (=\u2009start) and at the end of hypoxia or at 40\u201345\u00a0min for the sham-operated control group. Applying the qRT-PCR method, ncfDNA concentrations in piglets exposed to hypoxia revealed an increasing trend from 7.1\u00a0ng\/ml to 9.5\u00a0ng\/ml for <jats:italic>HK2<\/jats:italic> (hexokinase 2) and from 4.6\u00a0ng\/ml to 7.9\u00a0ng\/ml for \u03b2-globulin, respectively, whereas the control animals showed a more balanced profile. Furthermore, median levels of mtcfDNA were much higher in comparison to ncfDNA, but without significant differences between intervention versus the control group.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Both, qRT-PCR and the droplet digital PCR technique identified overall similar patterns for the concentration changes of cfDNA; but, the more sensitive digital PCR methodology might be required to identify minimal responses.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s11033-022-08135-0","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T12:04:07Z","timestamp":1670933047000},"page":"1533-1544","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia"],"prefix":"10.1007","volume":"50","author":[{"given":"Marie","family":"Bitenc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedicte","family":"Grebstad Tune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Melheim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Atneosen-\u00c5segg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoran","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polona","family":"Rajar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00f8nnaug","family":"Solberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1290-312X","authenticated-orcid":false,"given":"Lars Oliver","family":"Baumbusch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,12,13]]},"reference":[{"key":"8135_CR1","doi-asserted-by":"publisher","first-page":"1649","DOI":"10.1111\/brv.12413","volume":"93","author":"J Aucamp","year":"2018","unstructured":"Aucamp J, Bronkhorst AJ, Badenhorst CPS, Pretorius PJ (2018) The diverse origins of circulating cell-free DNA in the human body: a critical re-evaluation of the literature: the diverse origins of circulating cell-free DNA. 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