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This study aimed to explore potential factors related to no-call cfDNA test results and to discuss whether retest results are reliable.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods and results<\/jats:title>\n                <jats:p>This was a retrospective study of women with singleton pregnancies undergoing cfDNA testing in 2021. Of the 9871 pregnant patients undergoing cfDNA testing, 111 had a no-call result, and their results were compared to those of 170 control patients. The no-call rate was 1.12% (111\/9871), and the primary cause for no-call results was data fluctuation (88.29%, 98\/111). Medical conditions were significantly more frequent in the no-call group than in the reportable results group (P\u2009&lt;\u20090.001). After retesting, 107 (107\/111, 96.40%) patients had a result, and the false-positive rate (FPR) of retesting was 10.09% (10.09%, 11\/109). In addition, placental lesions were more frequent in the no-call group than in the reportable results group (P\u2009=\u20090.037), and 4 patients, all in the no-call group, experienced pregnancy loss.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Pregnant women with medical conditions are more likely to have a no-call result. A retest is suggested for patients with a no-call result, but retests have a high FPR. In addition, pregnant women with a no-call result are at increased risk of adverse pregnancy outcomes. In conclusion, more attention should be given to pregnant women for whom a no-call cfDNA result is obtained.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s11033-023-08591-2","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T13:01:41Z","timestamp":1691067701000},"page":"7649-7657","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of retest reliability for pregnant women undergoing cfDNA testing with a no-call result"],"prefix":"10.1007","volume":"50","author":[{"given":"Shuqiong","family":"He","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4311-967X","authenticated-orcid":false,"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meihuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangpu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"issue":"9076","key":"8591_CR1","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/S0140-6736(97)02174-0","volume":"350","author":"YM Lo","year":"1997","unstructured":"Lo YM, Corbetta N, Chamberlain PF et al (1997) Presence of fetal DNA in maternal plasma and serum. 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