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However, other chemicals are used during canola cultivation, including fertilizers and herbicides. There is widespread use of herbicides that target the acetolactate synthase (ALS) enzyme involved in branched chain amino acid synthesis and low levels of these amino acids within leaves of <jats:italic>Brassica<\/jats:italic> species. In <jats:italic>L. maculans<\/jats:italic> the <jats:italic>ilv2<\/jats:italic> gene encodes ALS and thus ALS-inhibiting herbicides may inadvertently impact the fungus.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods and results<\/jats:title>\n            <jats:p>Here, the impact of a commercial herbicide targeting ALS and mutation of the homologous <jats:italic>ilv2<\/jats:italic> gene in <jats:italic>L. maculans<\/jats:italic> was explored. Exposure to herbicide had limited impact on growth in vitro but reduced lesion sizes in plant disease experiments. Furthermore, the mutation of the <jats:italic>ilv2<\/jats:italic> gene via CRISPR-Cas9 gene editing rendered the fungus non-pathogenic.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Herbicide applications can influence disease outcome, but likely to a minor extent.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s11033-024-09620-4","type":"journal-article","created":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T19:01:32Z","timestamp":1716663692000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The ilv2 gene, encoding acetolactate synthase for branched chain amino acid biosynthesis, is required for plant pathogenicity by Leptosphaeria maculans"],"prefix":"10.1007","volume":"51","author":[{"given":"Nicholas F.","family":"Chong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela P.","family":"Van de Wouw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Idnurm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,25]]},"reference":[{"key":"9620_CR1","doi-asserted-by":"publisher","first-page":"4011","DOI":"10.1093\/nar\/13.11.4011","volume":"13","author":"SC Falco","year":"1985","unstructured":"Falco SC, Dumas KS, Livak KJ (1985) Nucleotide sequence of the yeast ILV2 gene which encodes acetolactate synthase. 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