{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T20:40:00Z","timestamp":1781124000432,"version":"3.54.1"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T00:00:00Z","timestamp":1720828800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T00:00:00Z","timestamp":1720828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"DST-SYST, New Delhi","award":["SP\/YO\/2019\/1046(G) dated 20.5.2020"],"award-info":[{"award-number":["SP\/YO\/2019\/1046(G) dated 20.5.2020"]}]},{"name":"DST-SYST, New Delhi","award":["SP\/YO\/2019\/1046(G) dated 20.5.2020"],"award-info":[{"award-number":["SP\/YO\/2019\/1046(G) dated 20.5.2020"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1007\/s11033-024-09705-0","type":"journal-article","created":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T06:02:21Z","timestamp":1720850541000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Loop-mediated isothermal amplification (LAMP) assay for early on-site detection of Group B Streptococcus infection in neonatal sepsis blood sample"],"prefix":"10.1007","volume":"51","author":[{"given":"Shukla","family":"Banerjee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mithun H.","family":"K.","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8627-9759","authenticated-orcid":false,"given":"Rajesh P.","family":"Shastry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,13]]},"reference":[{"key":"9705_CR1","doi-asserted-by":"publisher","first-page":"11930","DOI":"10.3390\/ijms231911930","volume":"23","author":"L Barcellini","year":"2022","unstructured":"Barcellini L, Ricci G, Bresesti I et al (2022) The role of virulence factors in neonatal Sepsis caused by enterobacterales: a systematic review. Int J Mol Sci 23:11930. https:\/\/doi.org\/10.3390\/ijms231911930","journal-title":"Int J Mol Sci"},{"key":"9705_CR2","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1007\/s12098-019-03106-z","volume":"87","author":"C Wattal","year":"2020","unstructured":"Wattal C, Kler N, Oberoi JK et al (2020) Neonatal Sepsis: mortality and morbidity in neonatal Sepsis due to Multidrug-Resistant (MDR) organisms: part 1. Indian J Pediatr 87:117\u2013121. https:\/\/doi.org\/10.1007\/s12098-019-03106-z","journal-title":"Indian J Pediatr"},{"key":"9705_CR3","volume-title":"Neonatal Sepsis","author":"M Singh","year":"2023","unstructured":"Singh M, Alsaleem M, Gray CP (2023) Neonatal Sepsis. In: StatPearls. StatPearls Publishing, Treasure Island (FL)"},{"key":"9705_CR4","doi-asserted-by":"publisher","first-page":"350","DOI":"10.3345\/cep.2021.00668","volume":"65","author":"WS Song","year":"2021","unstructured":"Song WS, Park HW, Oh MY et al (2021) Neonatal sepsis-causing bacterial pathogens and outcome of trends of their antimicrobial susceptibility a 20-year period at a neonatal intensive care unit. Clin Exp Pediatr 65:350\u2013357. https:\/\/doi.org\/10.3345\/cep.2021.00668","journal-title":"Clin Exp Pediatr"},{"key":"9705_CR5","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1007\/s00203-023-03653-2","volume":"205","author":"S Banerjee","year":"2023","unstructured":"Banerjee S, Bajire SK, Mithun HK, Shastry RP (2023) 3-(Bromoacetyl) coumarin is a potential therapeutic agent against neonatal sepsis-associated Pseudomonas extremorientalis. Arch Microbiol 205:312. https:\/\/doi.org\/10.1007\/s00203-023-03653-2","journal-title":"Arch Microbiol"},{"key":"9705_CR6","doi-asserted-by":"publisher","first-page":"2483","DOI":"10.3390\/microorganisms10122483","volume":"10","author":"Y Liu","year":"2022","unstructured":"Liu Y, Liu J (2022) Group B Streptococcus: virulence factors and pathogenic mechanism. Microorganisms 10:2483. https:\/\/doi.org\/10.3390\/microorganisms10122483","journal-title":"Microorganisms"},{"key":"9705_CR7","doi-asserted-by":"publisher","first-page":"e807","DOI":"10.1016\/S2214-109X(22)00093-6","volume":"10","author":"BP Gon\u00e7alves","year":"2022","unstructured":"Gon\u00e7alves BP, Procter SR, Paul P et al (2022) Group B streptococcus infection during pregnancy and infancy: estimates of regional and global burden. Lancet Glob Health 10:e807\u2013e819. https:\/\/doi.org\/10.1016\/S2214-109X(22)00093-6","journal-title":"Lancet Glob Health"},{"key":"9705_CR8","doi-asserted-by":"publisher","first-page":"235","DOI":"10.3390\/microorganisms11020235","volume":"11","author":"GA Sofouli","year":"2023","unstructured":"Sofouli GA, Tsintoni A, Fouzas S et al (2023) Early diagnosis of late-onset neonatal Sepsis using a Sepsis prediction score. Microorganisms 11:235. https:\/\/doi.org\/10.3390\/microorganisms11020235","journal-title":"Microorganisms"},{"key":"9705_CR9","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1038\/s41390-021-01696-z","volume":"91","author":"IH Celik","year":"2022","unstructured":"Celik IH, Hanna M, Canpolat FE, Pammi M (2022) Diagnosis of neonatal Sepsis: the past, Present and Future. Pediatr Res 91:337\u2013350. https:\/\/doi.org\/10.1038\/s41390-021-01696-z","journal-title":"Pediatr Res"},{"key":"9705_CR10","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/j.aca.2017.10.022","volume":"996","author":"Y Wang","year":"2017","unstructured":"Wang Y, Liu D, Deng J et al (2017) Loop-mediated isothermal amplification using self-avoiding molecular recognition systems and antarctic thermal sensitive uracil-DNA-glycosylase for detection of nucleic acid with prevention of carryover contamination. Anal Chim Acta 996:74\u201387. https:\/\/doi.org\/10.1016\/j.aca.2017.10.022","journal-title":"Anal Chim Acta"},{"key":"9705_CR11","doi-asserted-by":"crossref","unstructured":"Oliveira BB, Veigas B, Baptista PV (2021) Isothermal amplification of nucleic acids: the race for the Next Gold Standard. Front Sens 2","DOI":"10.3389\/fsens.2021.752600"},{"key":"9705_CR12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s42003-023-04612-9","volume":"6","author":"A Szobi","year":"2023","unstructured":"Szobi A, Buranovsk\u00e1 K, Vojta\u0161\u0161\u00e1kov\u00e1 N et al (2023) Vivid COVID-19 LAMP is an ultrasensitive, quadruplexed test using LNA-modified primers and a zinc ion and 5-Br-PAPS colorimetric detection system. Commun Biol 6:1\u201324. https:\/\/doi.org\/10.1038\/s42003-023-04612-9","journal-title":"Commun Biol"},{"key":"9705_CR13","doi-asserted-by":"publisher","first-page":"4725","DOI":"10.1128\/IAI.67.9.4725-4731.1999","volume":"67","author":"K Gase","year":"1999","unstructured":"Gase K, Ferretti JJ, Primeaux C, McShan WM (1999) Identification, Cloning, and expression of the CAMP factor gene (cfa) of Group A Streptococci. Infect Immun 67:4725\u20134731","journal-title":"Infect Immun"},{"key":"9705_CR14","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1139\/w00-078","volume":"46","author":"AA Hassan","year":"2000","unstructured":"Hassan AA, Abdulmawjood A, Yildirim AO et al (2000) Identification of Streptococci isolated from various sources by determination of cfb gene and other CAMP-factor genes. Can J Microbiol 46:946\u2013951","journal-title":"Can J Microbiol"},{"key":"9705_CR15","doi-asserted-by":"publisher","first-page":"1514","DOI":"10.1016\/j.jia.2022.10.004","volume":"22","author":"A Leghari","year":"2023","unstructured":"Leghari A, Lakho SA, Khand FM et al (2023) Molecular epidemiology, characterization of virulence factors and antibiotic resistance profile of Streptococcus agalactiae isolated from dairy farms in China and Pakistan. J Integr Agric 22:1514\u20131528. https:\/\/doi.org\/10.1016\/j.jia.2022.10.004","journal-title":"J Integr Agric"},{"key":"9705_CR16","doi-asserted-by":"publisher","unstructured":"Shi H, Zhou M, Zhang Z et al (2023) Molecular epidemiology, drug resistance, and virulence gene analysis of Streptococcus agalactiae isolates from dairy goats in backyard farms in China. Front Cell Infect Microbiol 12. https:\/\/doi.org\/10.3389\/fcimb.2022.1049167","DOI":"10.3389\/fcimb.2022.1049167"},{"key":"9705_CR17","doi-asserted-by":"publisher","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi T, Okayama H, Masubuchi H et al (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63. https:\/\/doi.org\/10.1093\/nar\/28.12.e63","journal-title":"Nucleic Acids Res"},{"key":"9705_CR18","doi-asserted-by":"publisher","first-page":"115","DOI":"10.3389\/fcimb.2020.00115","volume":"10","author":"HD de Paz","year":"2020","unstructured":"de Paz HD, Brotons P, Esteva C, Mu\u00f1oz-Almagro C (2020) Validation of a Loop-mediated isothermal amplification assay for Rapid diagnosis of Invasive Pneumococcal Disease. Front Cell Infect Microbiol 10:115. https:\/\/doi.org\/10.3389\/fcimb.2020.00115","journal-title":"Front Cell Infect Microbiol"},{"key":"9705_CR19","doi-asserted-by":"publisher","first-page":"fnv148","DOI":"10.1093\/femsle\/fnv148","volume":"362","author":"X Zhao","year":"2015","unstructured":"Zhao X, He X, Li H et al (2015) Detection of Streptococcus pyogenes using rapid visual molecular assay. FEMS Microbiol Lett 362:fnv148. https:\/\/doi.org\/10.1093\/femsle\/fnv148","journal-title":"FEMS Microbiol Lett"},{"key":"9705_CR20","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1038\/s41564-021-00870-7","volume":"6","author":"K Sands","year":"2021","unstructured":"Sands K, Carvalho MJ, Portal E et al (2021) Characterization of antimicrobial-resistant Gram-negative bacteria that cause neonatal sepsis in seven low- and middle-income countries. Nat Microbiol 6:512\u2013523. https:\/\/doi.org\/10.1038\/s41564-021-00870-7","journal-title":"Nat Microbiol"},{"key":"9705_CR21","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.mcna.2020.02.011","volume":"104","author":"MD Font","year":"2020","unstructured":"Font MD, Thyagarajan B, Khanna AK (2020) Sepsis and septic shock - basics of diagnosis, pathophysiology and clinical decision making. Med Clin North Am 104:573\u2013585. https:\/\/doi.org\/10.1016\/j.mcna.2020.02.011","journal-title":"Med Clin North Am"},{"key":"9705_CR22","doi-asserted-by":"publisher","first-page":"8647","DOI":"10.1007\/s00253-021-11637-9","volume":"105","author":"SK Bajire","year":"2021","unstructured":"Bajire SK, Jain S, Johnson RP, Shastry RP (2021) 6-Methylcoumarin attenuates quorum sensing and biofilm formation in Pseudomonas aeruginosa PAO1 and its applications on solid surface coatings with polyurethane. Appl Microbiol Biotechnol 105:8647\u20138661. https:\/\/doi.org\/10.1007\/s00253-021-11637-9","journal-title":"Appl Microbiol Biotechnol"},{"key":"9705_CR23","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1007\/s11274-023-03655-8","volume":"39","author":"SK Bajire","year":"2023","unstructured":"Bajire SK, Prabhu A, Bhandary YP et al (2023) 7-Ethoxycoumarin rescued Caenorhabditis elegans from infection of COPD derived clinical isolate Pseudomonas aeruginosa through virulence and biofilm inhibition via targeting Rhl and Pqs quorum sensing systems. World J Microbiol Biotechnol 39:208. https:\/\/doi.org\/10.1007\/s11274-023-03655-8","journal-title":"World J Microbiol Biotechnol"},{"key":"9705_CR24","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1080\/14787210.2020.1732818","volume":"18","author":"CR Popescu","year":"2020","unstructured":"Popescu CR, Cavanagh MMM, Tembo B et al (2020) Neonatal sepsis in low-income countries: epidemiology, diagnosis and prevention. Expert Rev Anti Infect Ther 18:443\u2013452. https:\/\/doi.org\/10.1080\/14787210.2020.1732818","journal-title":"Expert Rev Anti Infect Ther"},{"key":"9705_CR25","doi-asserted-by":"publisher","first-page":"544","DOI":"10.1111\/j.1469-0691.2009.02736.x","volume":"15","author":"H Westh","year":"2009","unstructured":"Westh H, Lisby G, Breysse F et al (2009) Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 15:544\u2013551. https:\/\/doi.org\/10.1111\/j.1469-0691.2009.02736.x","journal-title":"Clin Microbiol Infect"},{"key":"9705_CR26","doi-asserted-by":"publisher","first-page":"2009","DOI":"10.1007\/s00216-020-02490-2","volume":"412","author":"M Sidstedt","year":"2020","unstructured":"Sidstedt M, R\u00e5dstr\u00f6m P, Hedman J (2020) PCR inhibition in qPCR, dPCR and MPS\u2014mechanisms and solutions. Anal Bioanal Chem 412:2009\u20132023. https:\/\/doi.org\/10.1007\/s00216-020-02490-2","journal-title":"Anal Bioanal Chem"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-024-09705-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-024-09705-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-024-09705-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T03:48:21Z","timestamp":1738727301000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-024-09705-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,13]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["9705"],"URL":"https:\/\/doi.org\/10.1007\/s11033-024-09705-0","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,13]]},"assertion":[{"value":"31 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 June 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All participants were recruited from Yenepoya Medical College Hospital, Mangalore and approved by Yenepoya Ethics Committee-1 (Protocol number YEC-1\/2021\/024).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Informed consent was obtained from the mother\/father of the baby or a Legally Authorized representative (LAR) for the collection of blood. Samples were collected once after obtaining written informed consent. Subsequently, all the data was anonymized to facilitate analysis and the dissemination of results.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"811"}}