{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T04:15:58Z","timestamp":1777090558604,"version":"3.51.4"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Senior Research Support Program of Vietnam Academy of Science and Technology","award":["NVCC40.02\/23-24"],"award-info":[{"award-number":["NVCC40.02\/23-24"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1007\/s11033-024-10015-8","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T15:03:18Z","timestamp":1729522998000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring gene mutations and multidrug resistance in Mycobacterium tuberculosis: a study from the Lung Hospital in Vietnam"],"prefix":"10.1007","volume":"51","author":[{"given":"Thuy Thi Bich","family":"Vo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diem Thi","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuan Chi","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoan Thi","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hop Thi","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minh Ngoc","family":"Nghiem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"10015_CR1","volume-title":"Global tuberculosis report","author":"World Health Organization","year":"2023","unstructured":"World Health Organization (2023) Global tuberculosis report. World Health Organization, WHO"},{"issue":"4","key":"10015_CR2","doi-asserted-by":"publisher","first-page":"e00180","DOI":"10.1128\/cmr.00180-19","volume":"35","author":"N Dookie","year":"2022","unstructured":"Dookie N, Ngema SL, Perumal R, Naicker N, Padayatchi N, Naidoo K (2022) The changing paradigm of drug-resistant tuberculosis treatment: successes, pitfalls, and future perspectives. Clin Microbiol Rev 35(4):e00180\u2013e00119","journal-title":"Clin Microbiol Rev"},{"key":"10015_CR3","doi-asserted-by":"publisher","first-page":"102343","DOI":"10.1016\/j.tube.2023.102343","volume":"140","author":"MNF Noorizhab","year":"2023","unstructured":"Noorizhab MNF, Abidin NZ, Teh LK, Tang TH, Onyejepu N, Kunle-Ope C, Tochukwu NE, Sheshi MA, Nwafor T, Akinwale OP (2023) Exploration of the diversity of multi-drug resistant Mycobacterium tuberculosis complex in Lagos, Nigeria using WGS: distribution of lineages, drug resistance patterns and genetic mutations. Tuberculosis 140:102343","journal-title":"Tuberculosis"},{"issue":"1","key":"10015_CR4","doi-asserted-by":"publisher","first-page":"27","DOI":"10.3390\/pathogens13010027","volume":"13","author":"K Naidoo","year":"2023","unstructured":"Naidoo K, Perumal R, Ngema SL, Shunmugam L, Somboro AM (2023) Rapid diagnosis of drug-resistant tuberculosis\u2013opportunities and challenges. Pathogens 13(1):27","journal-title":"Pathogens"},{"key":"10015_CR5","unstructured":"Vietnam Ministry of Health (2024) Decision to promulgate guidelines for diagnosis, treatment and prevention of tuberculosis. Bo Y Te. (Vietnamese Language) 162\/QD-BYT"},{"key":"10015_CR6","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.jhin.2022.01.024","volume":"122","author":"DW Eyre","year":"2022","unstructured":"Eyre DW (2022) Infection prevention and control insights from a decade of pathogen whole-genome sequencing. J Hosp Infect 122:180\u2013186","journal-title":"J Hosp Infect"},{"issue":"6","key":"10015_CR7","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/S1473-3099(18)30073-2","volume":"18","author":"M Zignol","year":"2018","unstructured":"Zignol M, Cabibbe AM, Dean AS, Glaziou P, Alikhanova N, Ama C, Andres S, Barbova A, Borbe-Reyes A, Chin DP (2018) Genetic sequencing for surveillance of drug resistance in tuberculosis in highly endemic countries: a multi-country population-based surveillance study. Lancet Infect Dis 18(6):675\u2013683","journal-title":"Lancet Infect Dis"},{"issue":"9","key":"10015_CR8","doi-asserted-by":"publisher","first-page":"a017863","DOI":"10.1101\/cshperspect.a017863","volume":"5","author":"KJ Seung","year":"2015","unstructured":"Seung KJ, Keshavjee S, Rich ML (2015) Multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis. Cold Spring Harbor Perspect Med 5(9):a017863","journal-title":"Cold Spring Harbor Perspect Med"},{"key":"10015_CR9","doi-asserted-by":"crossref","unstructured":"Baku\u0142a Z, Napi\u00f3rkowska A, Bielecki J, Augustynowicz-Kope\u0107 E, Zwolska Z, Jagielski T (2013) Mutations in the embB gene and their association with ethambutol resistance in multidrug-resistant Mycobacterium tuberculosis clinical isolates from Poland. Biomed Res Int 2013(1):167954","DOI":"10.1155\/2013\/167954"},{"key":"10015_CR10","unstructured":"Vietnam Ministry of Health (2018) Guidelines for technical procedures in the field of Microbiology. Bo Y Te (Vietnamese Language) 6769\/QD-BYT"},{"issue":"1","key":"10015_CR11","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1054\/tuld.1998.0002","volume":"79","author":"S Ramaswamy","year":"1998","unstructured":"Ramaswamy S, Musser JM (1998) Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuber Lung Dis 79(1):3\u201329","journal-title":"Tuber Lung Dis"},{"issue":"6","key":"10015_CR12","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1016\/S0092-8674(01)00286-0","volume":"104","author":"EA Campbell","year":"2001","unstructured":"Campbell EA, Korzheva N, Mustaev A, Murakami K, Nair S, Goldfarb A, Darst SA (2001) Structural mechanism for rifampicin inhibition of bacterial RNA polymerase. Cell 104(6):901\u2013912","journal-title":"Cell"},{"issue":"1","key":"10015_CR13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/emi.2014.17","volume":"3","author":"A Koch","year":"2014","unstructured":"Koch A, Mizrahi V, Warner DF (2014) The impact of drug resistance on Mycobacterium tuberculosis physiology: what can we learn from rifampicin? Emerg Microbes Infections 3(1):1\u201311","journal-title":"Emerg Microbes Infections"},{"issue":"4","key":"10015_CR14","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.1128\/jcm.32.4.1095-1098.1994","volume":"32","author":"V Kapur","year":"1994","unstructured":"Kapur V, Li LL, Iordanescu S, Hamrick MR, Wanger A, Kreiswirth BN, Musser JM (1994) Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas. J Clin Microbiol 32(4):1095\u20131098","journal-title":"J Clin Microbiol"},{"issue":"3","key":"10015_CR15","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/S0895-3988(10)60051-7","volume":"23","author":"GL Li","year":"2010","unstructured":"Li GL, Zhao DF, Xie T, Ju H, Mu C, Zhao H, Wang X (2010) Molecular characterization of drug-resistant Beijing family isolates of Mycobacterium tuberculosis from Tianjin, China. Biomed Environ Sci 23(3):188\u2013193","journal-title":"Biomed Environ Sci"},{"issue":"4","key":"10015_CR16","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1128\/JCM.37.4.1197-1199.1999","volume":"37","author":"G Pozzi","year":"1999","unstructured":"Pozzi G, Meloni M, Iona E, Orr\u00f9 G, Thoresen O, Ricci M, Oggioni M, Fattorini L, Orefici G (1999) rpoB mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy. J Clin Microbiol 37(4):1197\u20131199","journal-title":"J Clin Microbiol"},{"issue":"12","key":"10015_CR17","doi-asserted-by":"publisher","first-page":"3844","DOI":"10.1128\/JCM.37.12.3844-3850.1999","volume":"37","author":"LK Yuen","year":"1999","unstructured":"Yuen LK, Leslie D, Coloe PJ (1999) Bacteriological and molecular analysis of rifampin-resistant Mycobacterium tuberculosis strains isolated in Australia. J Clin Microbiol 37(12):3844\u20133850","journal-title":"J Clin Microbiol"},{"issue":"5","key":"10015_CR18","doi-asserted-by":"publisher","first-page":"e0232343","DOI":"10.1371\/journal.pone.0232343","volume":"15","author":"M Rowneki","year":"2020","unstructured":"Rowneki M, Aronson N, Du P, Sachs P, Blakemore R, Chakravorty S, Levy S, Jones AL, Trivedi G, Chebore S (2020) Detection of drug resistant Mycobacterium tuberculosis by high-throughput sequencing of DNA isolated from acid fast bacilli smears. PLoS ONE 15(5):e0232343","journal-title":"PLoS ONE"},{"issue":"1","key":"10015_CR19","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/s40249-020-00652-z","volume":"9","author":"L Wan","year":"2020","unstructured":"Wan L, Guo Q, Wei JH, Liu HC, Li M-C, Jiang Y, Zhao LL, Zhao XQ, Liu ZG, Wan KL (2020) Accuracy of a reverse dot blot hybridization assay for simultaneous detection of the resistance of four anti-tuberculosis drugs in Mycobacterium tuberculosis isolated from China. Infect Dis Poverty 9(1):38","journal-title":"Infect Dis Poverty"},{"key":"10015_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12879-018-3638-z","volume":"19","author":"NM Al-Mutairi","year":"2019","unstructured":"Al-Mutairi NM, Ahmad S, Mokaddas E, Eldeen HS, Joseph S (2019) Occurrence of disputed rpoB mutations among Mycobacterium tuberculosis isolates phenotypically susceptible to rifampicin in a country with a low incidence of multidrug-resistant tuberculosis. BMC Infect Dis 19:1\u20139","journal-title":"BMC Infect Dis"},{"issue":"4","key":"10015_CR21","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1093\/jac\/dkq519","volume":"66","author":"GKH Siu","year":"2011","unstructured":"Siu GKH, Zhang Y, Lau TC, Lau RW, Ho P-L, Yew W-W, Tsui SK, Cheng VC, Yuen K-Y, Yam W-C (2011) Mutations outside the rifampicin resistance-determining region associated with rifampicin resistance in Mycobacterium tuberculosis. J Antimicrob Chemother 66(4):730\u2013733","journal-title":"J Antimicrob Chemother"},{"key":"10015_CR22","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1016\/j.meegid.2016.09.004","volume":"45","author":"AN Unissa","year":"2016","unstructured":"Unissa AN, Subbian S, Hanna LE, Selvakumar N (2016) Overview on mechanisms of isoniazid action and resistance in Mycobacterium tuberculosis. Infect Genet Evol 45:474\u2013492","journal-title":"Infect Genet Evol"},{"key":"10015_CR23","doi-asserted-by":"crossref","unstructured":"Zhang Y, Vilch\u00e8ze C, Jacobs JRWR (2004) Mechanisms of drug resistance in Mycobacterium tuberculosis. Tuberculosis and the tubercle bacillus:115\u2013140","DOI":"10.1128\/9781555817657.ch8"},{"key":"10015_CR24","doi-asserted-by":"crossref","unstructured":"Nagel R, Thomas JA, Adekunle FA, Mann FM, Peters RJ (2018) Arginine in the FARM and SARM: a role in chain-length determination for Arginine in the Aspartate-Rich motifs of Isoprenyl Diphosphate synthases from Mycobacterium tuberculosis. Molecules 23(10)","DOI":"10.3390\/molecules23102546"},{"issue":"8","key":"10015_CR25","doi-asserted-by":"publisher","first-page":"2640","DOI":"10.1128\/AAC.00112-06","volume":"50","author":"MH Hazb\u00f3n","year":"2006","unstructured":"Hazb\u00f3n MH, Brimacombe M, Bobadilla del Valle M, Cavatore M, Guerrero MI, Varma-Basil M, Billman-Jacobe H, Lavender C, Fyfe J, Garc\u00eda-Garc\u00eda L (2006) Population genetics study of isoniazid resistance mutations and evolution of multidrug-resistant Mycobacterium tuberculosis. Antimicrob Agents Chemother 50(8):2640\u20132649","journal-title":"Antimicrob Agents Chemother"},{"issue":"3","key":"10015_CR26","first-page":"344","volume":"15","author":"B M\u00fcller","year":"2011","unstructured":"M\u00fcller B, Streicher E, Hoek K, Tait M, Trollip A, Bosman M, Coetzee G, Chabula-Nxiweni E, Hoosain E, van Gey N (2011) inhA promoter mutations: a gateway to extensively drug-resistant tuberculosis in South Africa? Int J Tuberc Lung Dis 15(3):344\u2013351","journal-title":"Int J Tuberc Lung Dis"},{"issue":"1","key":"10015_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/emi.2017.73","volume":"6","author":"NQ Huy","year":"2017","unstructured":"Huy NQ, Lucie C, Hoa TTT, Hung NV, Lan NTN, Son NT, Nhung NV, Anh DD, Anne-Laure B, Van Anh NT (2017) Molecular analysis of pyrazinamide resistance in Mycobacterium tuberculosis in Vietnam highlights the high rate of pyrazinamide resistance-associated mutations in clinical isolates: PncA mutations and PZA resistance in M. Tuberculosis in Vietnam. Emerg Microbes Infections 6(1):1\u20137","journal-title":"Emerg Microbes Infections"},{"issue":"4","key":"10015_CR28","doi-asserted-by":"publisher","first-page":"2045","DOI":"10.1128\/AAC.04933-14","volume":"59","author":"LL Zhao","year":"2015","unstructured":"Zhao LL, Sun Q, Liu HC, Wu XC, Xiao TY, Zhao XQ, Li GL, Jiang Y, Zeng CY, Wan KL (2015) Analysis of embCAB mutations associated with ethambutol resistance in multidrug-resistant Mycobacterium tuberculosis isolates from China. Antimicrob Agents Chemother 59(4):2045\u20132050","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-024-10015-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-024-10015-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-024-10015-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T04:40:35Z","timestamp":1738730435000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-024-10015-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,21]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10015"],"URL":"https:\/\/doi.org\/10.1007\/s11033-024-10015-8","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,21]]},"assertion":[{"value":"22 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 October 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Ethical approval for this study was given by the Ethics Committee of Institute of Genome Research, Vietnam Academy of Science and Technology on 28 December 2022 (Ref. No.: 2432\/QD-VHL) and Vietnam Military Medical Academy; Military Hospital 103, Vietnam on 12 December 2022 (Ref. No. 04\/2022\/CNChTY-HDDD).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit .","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Open Access"}}],"article-number":"1084"}}