{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:28:31Z","timestamp":1787668111473,"version":"build-2736575974"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T00:00:00Z","timestamp":1734134400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T00:00:00Z","timestamp":1734134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Dokuz Eylul University Scientific Research Project Coordination Unit","award":["2021.KB.SA\u011e.036"],"award-info":[{"award-number":["2021.KB.SA\u011e.036"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11033-024-10158-8","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T02:37:13Z","timestamp":1734143833000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A simple and efficient \u201ccell in situ collagen zymography\u201d technique to evaluate cellular collagenase activities in thyroid cancer cell lines"],"prefix":"10.1007","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0188-7013","authenticated-orcid":false,"given":"Ege Gokce","family":"Savas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4985-9022","authenticated-orcid":false,"given":"Seniz Inanc","family":"Surer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1993-6741","authenticated-orcid":false,"given":"Murat","family":"Sipahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7350-8049","authenticated-orcid":false,"given":"Didem","family":"Keles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0481-6029","authenticated-orcid":false,"given":"Gulgun","family":"Oktay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,14]]},"reference":[{"key":"10158_CR1","doi-asserted-by":"publisher","first-page":"1251","DOI":"10.3390\/ph17091251","volume":"17","author":"W Andryszkiewicz","year":"2024","unstructured":"Andryszkiewicz W, Misi\u0105g P, Karwowska A, Resler Z, Wojno A, Kulbacka J, Szewczyk A, Rembia\u0142kowska N (2024) Cancer metastases to the liver: mechanisms of tumor cell colonization. Pharmaceuticals 17:1251","journal-title":"Pharmaceuticals"},{"key":"10158_CR2","doi-asserted-by":"publisher","first-page":"896099","DOI":"10.3389\/fmolb.2022.896099","volume":"9","author":"S Mustafa","year":"2022","unstructured":"Mustafa S, Koran S, AlOmair L (2022) Insights into the role of Matrix metalloproteinases in Cancer and its various therapeutic aspects: a review. Front Mol Biosci 9:896099","journal-title":"Front Mol Biosci"},{"key":"10158_CR3","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.biochi.2005.01.007","volume":"87","author":"C Lombard","year":"2005","unstructured":"Lombard C, Saulnier Jl, Wallach J (2005) Assays of matrix metalloproteinases (MMPs) activities: a review. Biochimie 87:265\u2013272","journal-title":"Biochimie"},{"issue":"1","key":"10158_CR4","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1369\/jhc.2009.954354","volume":"58","author":"E Hadler-Olsen","year":"2010","unstructured":"Hadler-Olsen E, Kanapathippillai P, Berg E, Svineng G, Winberg J-O, Uhlin-Hansen L (2010) Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity. J Histochem Cytochemistry 58(1):29\u201339","journal-title":"J Histochem Cytochemistry"},{"issue":"8","key":"10158_CR5","first-page":"1281","volume":"54","author":"S Ricci","year":"2015","unstructured":"Ricci S, D\u2019Esposito V, Oriente F, Formisano P, Di Carlo A (2015) Substrate-zymography: a still worthwhile method for gelatinases analysis in biological samples. Clin Chem Lab Med 54(8):1281\u20131290","journal-title":"Clin Chem Lab Med"},{"key":"10158_CR6","doi-asserted-by":"publisher","first-page":"488","DOI":"10.5152\/balkanmedj.2016.151304","volume":"33","author":"F Ho\u015fg\u00f6rler","year":"2016","unstructured":"Ho\u015fg\u00f6rler F, Kele\u015f D, Tanr\u0131verdi-Akhisaro\u011flu, \u0130nan\u00e7 \u015e, Akhisaro\u011flu M, Cankurt \u00dc, Aydo\u011fdu Z, U\u00e7ar AD, \u00c7etinayak O, Oktay G et al (2016) Anti-inflammatory and anti-apoptotic effect of valproic acid and doxycycline Independent from MMP Inhibition in Early Radiation damage. Balkan Med J 33:488\u2013495","journal-title":"Balkan Med J"},{"key":"10158_CR7","doi-asserted-by":"publisher","first-page":"110730","DOI":"10.1016\/j.cbi.2023.110730","volume":"385","author":"D Kele\u015f","year":"2023","unstructured":"Kele\u015f D, Sipahi M, \u0130nan\u00e7-S\u00fcrer \u015e, Djamgoz MB, Oktay G (2023) Tetracaine downregulates matrix metalloproteinase activity and inhibits invasiveness of strongly metastatic MDA-MB-231 human breast cancer cells. Chem Biol Interact 385:110730","journal-title":"Chem Biol Interact"},{"key":"10158_CR8","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1136\/jcp.2006.039297","volume":"60","author":"H Zhang","year":"2007","unstructured":"Zhang H, Xu L, Xiao D, Xie J, Zeng H, Wang Z, Zhang X, Niu Y, Shen Z, Shen J et al (2007) Upregulation of neutrophil gelatinase-associated lipocalin in oesophageal squamous cell carcinoma: significant correlation with cell differentiation and tumour invasion. J Clin Pathol 60:555\u2013561","journal-title":"J Clin Pathol"},{"key":"10158_CR9","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s12891-016-0892-3","volume":"17","author":"N Frara","year":"2016","unstructured":"Frara N, Abdelmagid SM, Tytell M, Amin M, Popoff SN, Safadi FF, Barbe MF (2016) Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping. BMC Musculoskelet Disord 17:34","journal-title":"BMC Musculoskelet Disord"},{"issue":"7","key":"10158_CR10","doi-asserted-by":"publisher","first-page":"3286","DOI":"10.1210\/en.2008-0250","volume":"149","author":"CS Samuel","year":"2008","unstructured":"Samuel CS, Hewitson TD, Zhang Y, Kelly DJ (2008) Relaxin ameliorates Fibrosis in Experimental Diabetic Cardiomyopathy. Endocrinology 149(7):3286\u20133293","journal-title":"Endocrinology"},{"key":"10158_CR11","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1186\/1756-0500-5-686","volume":"5","author":"MJ Veidal SSr, Nielsen","year":"2012","unstructured":"Veidal SSr, Nielsen MJ, Leeming DJ, Karsdal MA (2012) Phosphodiesterase inhibition mediates matrix metalloproteinase activity and the level of collagen degradation fragments in a liver fibrosis ex vivo rat model. BMC Res Notes 5:686","journal-title":"BMC Res Notes"},{"key":"10158_CR12","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1186\/s13104-015-1284-8","volume":"8","author":"J Christensen","year":"2015","unstructured":"Christensen J, Shastri VP (2015) Matrix-metalloproteinase-9 is cleaved and activated by Cathepsin K. BMC Res Notes 8:322","journal-title":"BMC Res Notes"},{"issue":"3","key":"10158_CR13","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1038\/nmeth.2371","volume":"10","author":"J Vandooren","year":"2013","unstructured":"Vandooren J, Geurts N, Martens E, Van den Steen PE, Opdenakker G (2013) Zymography methods for visualizing hydrolytic enzymes. Nat Methods 10(3):211\u2013220","journal-title":"Nat Methods"},{"issue":"3","key":"10158_CR14","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1354\/vp.40-3-227","volume":"40","author":"SJ Yan","year":"2003","unstructured":"Yan SJ, Blomme EAG (2003) In situ zymography: a Molecular Pathology technique to localize endogenous protease activity in tissue sections. Vet Pathol 40(3):227\u2013236","journal-title":"Vet Pathol"},{"key":"10158_CR15","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1007\/s11626-012-9529-5","volume":"48","author":"A Chhabra","year":"2012","unstructured":"Chhabra A, Jaiswal A, Malhotra U, Kohli S, Rani V (2012) Cell in situ zymography: an in vitro cytotechnology for localization of enzyme activity in cell culture. Vitro Cell Dev Biology - Anim 48:463\u2013468","journal-title":"Vitro Cell Dev Biology - Anim"},{"key":"10158_CR16","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/978-1-4939-7111-4_12","volume":"1626","author":"V Rani","year":"2017","unstructured":"Rani V, Chhabra A (2017) Cell in situ Zymography: imaging enzyme\u2013substrate interactions. Zymography: Methods Protocols 1626:133\u2013143","journal-title":"Zymography: Methods Protocols"},{"issue":"6","key":"10158_CR17","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1369\/jhc.4R6251.2004","volume":"52","author":"WM Frederiks","year":"2004","unstructured":"Frederiks WM, Mook ORF (2004) Metabolic mapping of proteinase activity with emphasis on in situ zymography of Gelatinases: review and protocols. J Histochem Cytochemistry 52(6):711\u2013722","journal-title":"J Histochem Cytochemistry"},{"issue":"8","key":"10158_CR18","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1177\/42.8.8027531","volume":"42","author":"JH Beckstead","year":"1994","unstructured":"Beckstead JH (1994) A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues. J Histochem Cytochemistry 42(8):1127\u20131134","journal-title":"J Histochem Cytochemistry"},{"issue":"6","key":"10158_CR19","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1097\/01.LAB.0000074892.53211.A5","volume":"83","author":"K Wester","year":"2003","unstructured":"Wester K, Asplund A, B\u00e4ckvall H, Micke P, Derveniece A, Hartmane I, Malmstr\u00f6m P-U, Pont\u00e9n F (2003) Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues. Lab Invest 83(6):889","journal-title":"Lab Invest"},{"issue":"4","key":"10158_CR20","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1016\/j.surg.2010.10.007","volume":"149","author":"EJ Mitmaker","year":"2011","unstructured":"Mitmaker EJ, Griff NJ, Grogan RH, Sarkar R, Kebebew E, Duh Q-Y, Clark OH, Shen WT (2011) Modulation of matrix metalloproteinase activity in human thyroid cancer cell lines using demethylating agents and histone deacetylase inhibitors. Surgery 149(4):504\u2013511","journal-title":"Surgery"},{"issue":"11 Pt A","key":"10158_CR21","doi-asserted-by":"publisher","first-page":"1952","DOI":"10.1016\/j.bbamcr.2017.03.010","volume":"1864","author":"P Cieplak","year":"2017","unstructured":"Cieplak P, Strongin AY (2017) Matrix metalloproteinases \u2013 from the cleavage data to the prediction tools and beyond. Biochim Biophys Acta 1864(11 Pt A):1952\u20131963","journal-title":"Biochim Biophys Acta"},{"key":"10158_CR22","doi-asserted-by":"publisher","first-page":"984","DOI":"10.3390\/cells8090984","volume":"8","author":"GB Fields","year":"2019","unstructured":"Fields GB (2019) The rebirth of Matrix Metalloproteinase inhibitors: moving beyond the Dogma. Cells 8:984","journal-title":"Cells"},{"issue":"5","key":"10158_CR23","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1517\/17460441.2011.560936","volume":"6","author":"N Sela-Passwell","year":"2011","unstructured":"Sela-Passwell N, Trahtenherts A, Kr\u00fcger A, Sagi I (2011) New opportunities in drug design of metalloproteinase inhibitors: combination between structure-function experimental approaches and systems biology. Expert Opin Drug Discov 6(5):527\u2013542","journal-title":"Expert Opin Drug Discov"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-024-10158-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-024-10158-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-024-10158-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T16:31:59Z","timestamp":1766853119000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-024-10158-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,14]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10158"],"URL":"https:\/\/doi.org\/10.1007\/s11033-024-10158-8","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,14]]},"assertion":[{"value":"25 October 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical approval for this study was obtained from the Ethics Review Board of Dokuz Eylul University (No: 2020\/26\u2009\u2212\u200915).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"49"}}