{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T14:47:03Z","timestamp":1772203623612,"version":"3.50.1"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2023A1515110325"],"award-info":[{"award-number":["2023A1515110325"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003453","name":"Guangdong Natural Science Foundation","doi-asserted-by":"crossref","award":["2023A1515012940"],"award-info":[{"award-number":["2023A1515012940"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Fundamental Research Funds for the Central Universities, Sun Yat-sen University","award":["23ptpy106"],"award-info":[{"award-number":["23ptpy106"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Biol Rep"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s11033-025-10392-8","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T01:30:52Z","timestamp":1741224652000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Choline metabolism disorder induced by Prevotella is a risk factor for endometrial cancer in women with polycystic ovary syndrome"],"prefix":"10.1007","volume":"52","author":[{"given":"Peigen","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianru","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"issue":"6","key":"10392_CR1","doi-asserted-by":"publisher","first-page":"2745","DOI":"10.1210\/jc.2003-032046","volume":"89","author":"R Azziz","year":"2004","unstructured":"Azziz R, Woods KS, Reyna R, Key TJ, Knochenhauer ES, Yildiz BO (2004) The prevalence and features of the polycystic ovary syndrome in an unselected population. J Clin Endocrinol Metab 89(6):2745\u20132749","journal-title":"J Clin Endocrinol Metab"},{"issue":"11","key":"10392_CR2","doi-asserted-by":"publisher","first-page":"4237","DOI":"10.1210\/jc.2006-0178","volume":"91","author":"R Azziz","year":"2006","unstructured":"Azziz R, Carmina E, Dewailly D, Diamanti-Kandarakis E, Escobar-Morreale HF, Futterweit W et al (2006) Positions statement: criteria for defining polycystic ovary syndrome as a predominantly hyperandrogenic syndrome: an androgen excess society guideline. J Clin Endocrinol Metab 91(11):4237\u20134245","journal-title":"J Clin Endocrinol Metab"},{"issue":"10","key":"10392_CR3","doi-asserted-by":"publisher","first-page":"1210","DOI":"10.1111\/j.1471-0528.2006.01008.x","volume":"113","author":"FJ Broekmans","year":"2006","unstructured":"Broekmans FJ, Knauff EA, Valkenburg O, Laven JS, Eijkemans MJ, Fauser BC (2006) PCOS according to the Rotterdam consensus criteria: change in prevalence among WHO-II anovulation and association with metabolic factors. BJOG 113(10):1210\u20131217","journal-title":"BJOG"},{"issue":"5","key":"10392_CR4","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1093\/humupd\/dmu012","volume":"20","author":"JA Barry","year":"2014","unstructured":"Barry JA, Azizia MM, Hardiman PJ (2014) Risk of endometrial, ovarian and breast cancer in women with polycystic ovary syndrome: a systematic review and meta-analysis. Hum Reprod Update 20(5):748\u2013758","journal-title":"Hum Reprod Update"},{"issue":"3","key":"10392_CR5","first-page":"181","volume":"4","author":"AR Genazzani","year":"2001","unstructured":"Genazzani AR, Gadducci A, Gambacciani M (2001) Controversial issues in climacteric medicine II. Hormone replacement therapy and cancer. Int Menopause Soc Expert Workshop 4(3):181\u2013193 9\u201312 June 2001, Opera del Duomo, Pisa, Italy. Climacteric","journal-title":"Int Menopause Soc Expert Workshop"},{"issue":"5","key":"10392_CR6","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1093\/humrep\/des042","volume":"27","author":"Z Haoula","year":"2012","unstructured":"Haoula Z, Salman M, Atiomo W (2012) Evaluating the association between endometrial cancer and polycystic ovary syndrome. Hum Reprod 27(5):1327\u20131331","journal-title":"Hum Reprod"},{"issue":"2","key":"10392_CR7","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.ygyno.2013.06.032","volume":"131","author":"MN Shafiee","year":"2013","unstructured":"Shafiee MN, Chapman C, Barrett D, Abu J, Atiomo W (2013) Reviewing the molecular mechanisms which increase endometrial cancer (EC) risk in women with polycystic ovarian syndrome (PCOS): time for paradigm shift? Gynecol Oncol 131(2):489\u2013492","journal-title":"Gynecol Oncol"},{"issue":"1","key":"10392_CR8","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.ygyno.2015.04.036","volume":"138","author":"D Chase","year":"2015","unstructured":"Chase D, Goulder A, Zenhausern F, Monk B, Herbst-Kralovetz M (2015) The vaginal and Gastrointestinal microbiomes in gynecologic cancers: a review of applications in etiology, symptoms and treatment. Gynecol Oncol 138(1):190\u2013200","journal-title":"Gynecol Oncol"},{"key":"10392_CR9","doi-asserted-by":"crossref","unstructured":"Barczynski B, Fraszczak K, Grywalska E, Kotarski J, Korona-Glowniak I (2023) Vaginal and cervical microbiota composition in patients with endometrial Cancer. Int J Mol Sci. 24(9)","DOI":"10.3390\/ijms24098266"},{"key":"10392_CR10","doi-asserted-by":"publisher","first-page":"727286","DOI":"10.3389\/fcell.2021.727286","volume":"9","author":"P Chen","year":"2021","unstructured":"Chen P, Guo Y, Jia L, Wan J, He T, Fang C et al (2021) Interaction between functionally activate endometrial microbiota and host gene regulation in endometrial Cancer. Front Cell Dev Biol 9:727286","journal-title":"Front Cell Dev Biol"},{"issue":"6230","key":"10392_CR11","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1126\/science.aaa4972","volume":"348","author":"WS Garrett","year":"2015","unstructured":"Garrett WS (2015) Cancer and the microbiota. Science 348(6230):80\u201386","journal-title":"Science"},{"key":"10392_CR12","doi-asserted-by":"publisher","first-page":"708838","DOI":"10.3389\/fendo.2021.708838","volume":"12","author":"Z Lei","year":"2021","unstructured":"Lei Z, Wu H, Yang Y, Hu Q, Lei Y, Liu W et al (2021) Ovariectomy impaired hepatic glucose and lipid homeostasis and altered the gut microbiota in mice with different diets. Front Endocrinol (Lausanne) 12:708838","journal-title":"Front Endocrinol (Lausanne)"},{"issue":"1","key":"10392_CR13","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/s40168-017-0228-z","volume":"5","author":"EA Miller","year":"2017","unstructured":"Miller EA, Livermore JA, Alberts SC, Tung J, Archie EA (2017) Ovarian cycling and reproductive state shape the vaginal microbiota in wild baboons. Microbiome 5(1):8","journal-title":"Microbiome"},{"issue":"1","key":"10392_CR14","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1093\/humrep\/deh098","volume":"19","author":"EA-SP Rotterdam","year":"2004","unstructured":"Rotterdam EA-SP (2004) Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod 19(1):41\u201347","journal-title":"Hum Reprod"},{"issue":"16","key":"10392_CR15","doi-asserted-by":"publisher","first-page":"5116","DOI":"10.1128\/AEM.01403-14","volume":"80","author":"LW Hugerth","year":"2014","unstructured":"Hugerth LW, Wefer HA, Lundin S, Jakobsson HE, Lindberg M, Rodin S et al (2014) DegePrime, a program for degenerate primer design for broad-taxonomic-range PCR in microbial ecology studies. Appl Environ Microbiol 80(16):5116\u20135123","journal-title":"Appl Environ Microbiol"},{"issue":"7","key":"10392_CR16","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","volume":"13","author":"BJ Callahan","year":"2016","unstructured":"Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP (2016) DADA2: High-resolution sample inference from illumina amplicon data. Nat Methods 13(7):581\u2013583","journal-title":"Nat Methods"},{"issue":"1","key":"10392_CR17","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1186\/s40168-018-0470-z","volume":"6","author":"NA Bokulich","year":"2018","unstructured":"Bokulich NA, Kaehler BD, Rideout JR, Dillon M, Bolyen E, Knight R et al (2018) Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2\u2019s q2-feature-classifier plugin. Microbiome 6(1):90","journal-title":"Microbiome"},{"issue":"Database issue","key":"10392_CR18","first-page":"D590","volume":"41","author":"C Quast","year":"2013","unstructured":"Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P et al (2013) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41(Database issue):D590\u2013D596","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"10392_CR19","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/s41587-020-0548-6","volume":"38","author":"GM Douglas","year":"2020","unstructured":"Douglas GM, Maffei VJ, Zaneveld JR, Yurgel SN, Brown JR, Taylor CM et al (2020) PICRUSt2 for prediction of metagenome functions. Nat Biotechnol 38(6):685\u2013688","journal-title":"Nat Biotechnol"},{"issue":"11","key":"10392_CR20","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","volume":"13","author":"P Shannon","year":"2003","unstructured":"Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D et al (2003) Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res 13(11):2498\u20132504","journal-title":"Genome Res"},{"issue":"11","key":"10392_CR21","doi-asserted-by":"publisher","first-page":"e1009442","DOI":"10.1371\/journal.pcbi.1009442","volume":"17","author":"H Mallick","year":"2021","unstructured":"Mallick H, Rahnavard A, McIver LJ, Ma S, Zhang Y, Nguyen LH et al (2021) Multivariable association discovery in population-scale meta-omics studies. PLoS Comput Biol 17(11):e1009442","journal-title":"PLoS Comput Biol"},{"issue":"8","key":"10392_CR22","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","volume":"37","author":"D Kim","year":"2019","unstructured":"Kim D, Paggi JM, Park C, Bennett C, Salzberg SL (2019) Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat Biotechnol 37(8):907\u2013915","journal-title":"Nat Biotechnol"},{"issue":"12","key":"10392_CR23","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S (2014) Moderated Estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15(12):550","journal-title":"Genome Biol"},{"issue":"3","key":"10392_CR24","first-page":"100141","volume":"2","author":"T Wu","year":"2021","unstructured":"Wu T, Hu E, Xu S, Chen M, Guo P, Dai Z et al (2021) ClusterProfiler 4.0: A universal enrichment tool for interpreting omics data. Innov (Camb) 2(3):100141","journal-title":"Innov (Camb)"},{"issue":"14","key":"10392_CR25","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1016\/j.vaccine.2013.10.010","volume":"32","author":"RM Brotman","year":"2014","unstructured":"Brotman RM, Ravel J, Bavoil PM, Gravitt PE, Ghanem KG (2014) Microbiome, sex hormones, and immune responses in the reproductive tract: challenges for vaccine development against sexually transmitted infections. Vaccine 32(14):1543\u20131552","journal-title":"Vaccine"},{"issue":"10","key":"10392_CR26","doi-asserted-by":"publisher","first-page":"2519","DOI":"10.1007\/s10815-021-02247-5","volume":"38","author":"P Punzon-Jimenez","year":"2021","unstructured":"Punzon-Jimenez P, Labarta E (2021) The impact of the female genital tract Microbiome in women health and reproduction: a review. J Assist Reprod Genet 38(10):2519\u20132541","journal-title":"J Assist Reprod Genet"},{"issue":"6","key":"10392_CR27","doi-asserted-by":"publisher","first-page":"1486","DOI":"10.1080\/10408398.2022.2117784","volume":"64","author":"N Wang","year":"2024","unstructured":"Wang N, Chen L, Yi K, Zhang B, Li C, Zhou X (2024) The effects of microbiota on reproductive health: A review. Crit Rev Food Sci Nutr 64(6):1486\u20131507","journal-title":"Crit Rev Food Sci Nutr"},{"issue":"9","key":"10392_CR28","doi-asserted-by":"publisher","first-page":"e1011658","DOI":"10.1371\/journal.ppat.1011658","volume":"19","author":"P Ghorbani","year":"2023","unstructured":"Ghorbani P, Kim SY, Smith TKT, Minarrieta L, Robert-Gostlin V, Kilgour MK et al (2023) Choline metabolism underpins macrophage IL-4 polarization and RELMalpha up-regulation in helminth infection. PLoS Pathog 19(9):e1011658","journal-title":"PLoS Pathog"},{"key":"10392_CR29","doi-asserted-by":"publisher","first-page":"9923204","DOI":"10.1155\/2022\/9923204","volume":"2022","author":"Y Gan","year":"2022","unstructured":"Gan Y, Long J, Zeng Y, Zhang Y, Tao Y, lncRNA (2022) IL-17RA-1 attenuates LPS-Induced Sepsis via miR-7847-3p\/PRKCG-Mediated MAPK signaling pathway. Mediators Inflamm 2022:9923204","journal-title":"Mediators Inflamm"},{"key":"10392_CR30","doi-asserted-by":"publisher","first-page":"115210","DOI":"10.1016\/j.biopha.2023.115210","volume":"165","author":"L Ji","year":"2023","unstructured":"Ji L, Song T, Ge C, Wu Q, Ma L, Chen X et al (2023) Identification of bioactive compounds and potential mechanisms of scutellariae radix-coptidis rhizoma in the treatment of atherosclerosis by integrating network Pharmacology and experimental validation. Biomed Pharmacother 165:115210","journal-title":"Biomed Pharmacother"},{"issue":"1","key":"10392_CR31","doi-asserted-by":"publisher","first-page":"18160","DOI":"10.1038\/s41598-021-97505-y","volume":"11","author":"A Skorupa","year":"2021","unstructured":"Skorupa A, Ponski M, Ciszek M, Cichon B, Klimek M, Witek A et al (2021) Grading of endometrial cancer using (1)H HR-MAS NMR-based metabolomics. Sci Rep 11(1):18160","journal-title":"Sci Rep"},{"issue":"23","key":"10392_CR32","doi-asserted-by":"publisher","first-page":"6867","DOI":"10.1158\/0008-5472.CAN-13-2409","volume":"74","author":"S Trousil","year":"2014","unstructured":"Trousil S, Lee P, Pinato DJ, Ellis JK, Dina R, Aboagye EO et al (2014) Alterations of choline phospholipid metabolism in endometrial cancer are caused by choline kinase alpha overexpression and a hyperactivated deacylation pathway. Cancer Res 74(23):6867\u20136877","journal-title":"Cancer Res"},{"key":"10392_CR33","first-page":"6458877","volume":"2022","author":"S Zhang","year":"2022","unstructured":"Zhang S, Han X (2022) The regulatory role of lipid metabolism in endometrial Cancer. J Oncol 2022:6458877","journal-title":"J Oncol"},{"key":"10392_CR34","doi-asserted-by":"crossref","unstructured":"Gelsomino L, Naimo GD, Catalano S, Mauro L, Ando S (2019) The emerging role of adiponectin in female malignancies. Int J Mol Sci. 20(9)","DOI":"10.3390\/ijms20092127"},{"issue":"11","key":"10392_CR35","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1038\/embor.2010.160","volume":"11","author":"H Gehart","year":"2010","unstructured":"Gehart H, Kumpf S, Ittner A, Ricci R (2010) MAPK signalling in cellular metabolism: stress or wellness? EMBO Rep 11(11):834\u2013840","journal-title":"EMBO Rep"}],"container-title":["Molecular Biology Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10392-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11033-025-10392-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11033-025-10392-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T16:32:09Z","timestamp":1766853129000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11033-025-10392-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,6]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10392"],"URL":"https:\/\/doi.org\/10.1007\/s11033-025-10392-8","relation":{},"ISSN":["0301-4851","1573-4978"],"issn-type":[{"value":"0301-4851","type":"print"},{"value":"1573-4978","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,6]]},"assertion":[{"value":"19 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All study procedures were reviewed and approved by the ethics review board of the Sixth Affiliated Hospital of Sun Yat-Sen University (IRB no. 2022ZSLYEC-370).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"285"}}