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Both species are endemic with very restricted distribution and are classified as Critically Endangered.\n                      <jats:italic>Caspiomyzon hellenicus<\/jats:italic>\n                      is restricted to Tenagi, Philippi and\n                      <jats:italic>C. graecus<\/jats:italic>\n                      to Louros River. No studies have characterized their mitochondrial genomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      The complete mitochondrial genome of\n                      <jats:italic>C. hellenicus<\/jats:italic>\n                      and\n                      <jats:italic>C. graecus<\/jats:italic>\n                      was generated with Oxford Nanopore long-read technology, and it was processed using various bioinformatics approaches. The final assembled contig length was 16,763\u00a0bp and 17,123\u00a0bp, respectively, and composed of 13 protein-coding genes, two ribosomal RNA genes, 22 tRNA genes, and two control regions. Repetitive sequences were detected between and within the control regions. The overall GC composition was approximately 36% for both species. The results of phylogenetics analysis using Bayesian inference and Maximum likelihood methods revealed that the\n                      <jats:italic>Geotria australis<\/jats:italic>\n                      species was sister to northern hemisphere lampreys, whereas\n                      <jats:italic>Mordacia<\/jats:italic>\n                      species constitutes a monophyletic group. Divergence time between the Greek\n                      <jats:italic>Caspiomyzon<\/jats:italic>\n                      species took place at approximately 0.7 Mya.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study enhanced our understanding of the taxonomic and phylogenetic relationships within the\n                      <jats:italic>Caspiomyzon<\/jats:italic>\n                      genus from Greece based on the characterization of the full mitochondrial genomes from long-reads technology. Such efforts can aid their conservation and management locally.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11033-025-10476-5","type":"journal-article","created":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T20:14:59Z","timestamp":1743884099000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterisation and phylogenetic analysis of the complete mitochondrial genome of two endemic lampreys from Greece (Caspiomyzon hellenicus and Caspiomyzon graecus) using Long-Read technology"],"prefix":"10.1007","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0866-6259","authenticated-orcid":false,"given":"Chrysoula","family":"Gubili","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orezia","family":"Seitidou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7030-5227","authenticated-orcid":false,"given":"Romina","family":"Batista","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3221-9125","authenticated-orcid":false,"given":"Paraskevi","family":"Papadopoulou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1676-4390","authenticated-orcid":false,"given":"Aris","family":"Christidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5818-7874","authenticated-orcid":false,"given":"Stelios","family":"Triantafillidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5028-4983","authenticated-orcid":false,"given":"Argyrios","family":"Sapounidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,5]]},"reference":[{"key":"10476_CR1","volume-title":"European red list of freshwater fishes","author":"J Freyhof","year":"2011","unstructured":"Freyhof J, Brooks E (2011) European red list of freshwater fishes. 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